FastQCFastQC Report
Fri 27 May 2016
SRR2140259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24866247
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA338300.050.3851931
GTATCAA605350.036.7547151
TCAACGC760350.028.5911164
ATCAACG768900.028.262133
CAACGCA779200.027.8830855
TATCAAC792400.027.767372
AACGCAG799800.027.2411526
ACGCAGA927550.023.4480297
CGCAGAG955900.022.7703958
GTACATG793650.022.2110541
TACATGG809200.021.3691442
ACATGGG815250.020.6733913
GCAGAGT1096250.019.7272159
CATGGGG597600.018.7188474
AGAGTAC986000.018.66548310-11
GAGTACT638500.018.31626312-13
CAGAGTA1065100.017.78808610-11
GTGGTAT150850.017.2212521
AGTACTT700100.016.67734312-13
GTACTTT722900.016.11694114-15
Produced by FastQC (version 0.11.5)