##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24866247
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.348618832588606	33.0	31.0	34.0	31.0	34.0
2	32.39143892522261	34.0	31.0	34.0	30.0	34.0
3	32.48153217491968	34.0	31.0	34.0	31.0	34.0
4	36.04873184119823	37.0	35.0	37.0	35.0	37.0
5	35.978608311901674	37.0	35.0	37.0	35.0	37.0
6	36.058287324178835	37.0	35.0	37.0	35.0	37.0
7	35.995883737501686	37.0	35.0	37.0	35.0	37.0
8	35.998907474859394	37.0	35.0	37.0	35.0	37.0
9	37.6945864408087	39.0	37.0	39.0	35.0	39.0
10-11	37.63192105346657	39.0	37.0	39.0	35.0	39.0
12-13	37.61927045122651	39.0	37.0	39.0	35.0	39.0
14-15	39.001287226817944	40.0	38.0	41.0	35.0	41.0
16-17	38.99685493351691	40.0	38.0	41.0	35.0	41.0
18-19	39.02530767912022	40.0	38.5	41.0	35.0	41.0
20-21	39.036764485118354	40.0	39.0	41.0	35.0	41.0
22-23	38.99569022341261	40.0	38.5	41.0	35.0	41.0
24-25	38.95995169021032	40.0	38.0	41.0	35.0	41.0
26-27	38.85621861974985	40.0	38.0	41.0	35.0	41.0
28-29	38.79917443516449	40.0	38.0	41.0	35.0	41.0
30-31	38.69981748378501	40.0	38.0	41.0	34.5	41.0
32-33	38.603673386171494	40.0	38.0	41.0	34.0	41.0
34-35	38.55219092940279	40.0	38.0	41.0	34.0	41.0
36-37	38.482702310019704	40.0	38.0	41.0	34.0	41.0
38-39	38.427503920893216	40.0	38.0	41.0	34.0	41.0
40-41	38.375918807272534	40.0	38.0	41.0	34.0	41.0
42-43	38.3040817364316	40.0	38.0	41.0	33.0	41.0
44-45	38.24986701335855	40.0	38.0	41.0	33.0	41.0
46-47	38.16839972340568	40.0	37.5	41.0	33.0	41.0
48-49	38.07476393736591	40.0	37.0	41.0	33.0	41.0
50-51	37.9629430808217	40.0	37.0	41.0	33.0	41.0
52-53	37.85791927843512	40.0	37.0	41.0	33.0	41.0
54-55	37.70771567746071	40.0	36.5	41.0	33.0	41.0
56-57	37.54244920681664	39.0	36.0	41.0	32.0	41.0
58-59	37.35564847279532	39.0	35.5	41.0	32.0	41.0
60-61	37.147323932992265	39.0	35.0	41.0	32.0	41.0
62-63	36.88963499776514	38.5	35.0	40.0	31.0	41.0
64-65	36.6305171672119	38.0	35.0	40.0	31.0	41.0
66-67	36.358017108500235	37.0	35.0	40.0	31.0	41.0
68-69	36.038608328117675	37.0	34.5	39.5	31.0	41.0
70-71	35.717390749421256	36.0	34.0	39.0	30.5	41.0
72-73	35.39622450579816	36.0	34.0	39.0	30.0	40.5
74-75	35.078177306643305	35.0	34.0	38.5	30.0	40.0
76-77	34.7545684185484	35.0	34.0	37.0	30.0	39.5
78-79	34.440147649598636	35.0	34.0	37.0	29.5	39.0
80-81	34.16669606917009	35.0	34.0	36.0	29.0	39.0
82-83	33.893751830119875	35.0	33.0	36.0	29.0	37.5
84-85	33.681222129732184	35.0	33.0	35.5	29.0	37.0
86-87	33.48569781019192	35.0	33.0	35.0	29.0	37.0
88-89	33.360644245130715	35.0	33.0	35.0	29.0	36.0
90-91	33.237089011437774	35.0	33.0	35.0	29.0	36.0
92-93	33.12257662125282	35.0	33.0	35.0	29.0	36.0
94-95	33.35952790055366	35.0	33.0	35.0	29.5	36.0
96-97	33.42354359759818	35.0	33.0	35.0	30.0	35.0
98-99	33.76006642348372	35.0	34.0	35.0	31.0	35.0
100	33.57183380394164	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	17.0
20	119.0
21	731.0
22	3485.0
23	11921.0
24	30735.0
25	65947.0
26	118147.0
27	187302.0
28	267214.0
29	356261.0
30	453495.0
31	565301.0
32	708513.0
33	910538.0
34	1235962.0
35	1754652.0
36	2825953.0
37	5195150.0
38	6584873.0
39	3432998.0
40	156932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79711156251283	19.947955958130713	13.58206970275812	24.672862776598333
2	14.94019759493842	21.34350193258132	40.57599577643956	23.1403046960407
3	17.87029220774651	26.779549805002738	30.408786657672948	24.941371329577798
4	12.567095468809589	18.09064311152383	38.45929383714398	30.882967582522607
5	13.00753579532952	38.151748945740906	35.47426674535142	13.366448513578149
6	29.794757528146487	38.2503117579424	18.64964584321872	13.305284870692388
7	26.05912745900095	32.679076983350164	23.713401543867878	17.54839401378101
8	24.64083542643166	36.59977719999323	21.053333862564784	17.706053511010328
9	26.026975441850958	15.9571727892834	20.838142563290713	37.177709205574935
10-11	24.483027133125475	26.398543374880816	28.97122352239162	20.14720596960209
12-13	25.39979796709974	24.10299994204996	28.99396318230089	21.503238908549406
14-15	22.5279210811346	25.064363351654954	26.525928902741136	25.88178666446931
16-17	22.05817588798181	28.111302843569437	27.13093978355479	22.69958148489396
18-19	22.214299166255365	27.30698967158172	28.812029816964333	21.666681345198572
20-21	23.06300725088893	26.467802738000294	28.596438877888534	21.872751133222238
22-23	22.815749553641673	26.390391432655512	28.478702167442304	22.31515684626051
24-25	22.525304726953838	26.58770775964414	28.557969820327845	22.329017693074178
26-27	22.480265010133277	26.620776624413622	28.525015661029073	22.373942704424028
28-29	22.497797889288993	26.552406730316612	28.548392265319894	22.4014031150745
30-31	22.72531476309501	26.501429110315343	28.374660270386308	22.39859585620334
32-33	22.537125924413797	26.725127420585725	28.333902482289474	22.403844172711004
34-35	22.648337393421546	26.701910234876376	28.339554303746574	22.310198067955504
36-37	22.76332362602417	26.63262468917371	28.19195983231857	22.412091852483552
38-39	22.62473723187039	26.76376663467482	28.209482246837876	22.402013886616917
40-41	22.814145541665155	26.580080484362554	28.13918420131888	22.466589772653407
42-43	22.647231473545666	26.744377991342716	27.932653140219156	22.67573739489247
44-45	22.72512468594064	26.853465230755173	27.70002822840658	22.721381854897608
46-47	22.936976936506635	26.90924308186977	27.338776078981926	22.81500390264167
48-49	22.907216534509093	27.028320579261838	27.188673050755945	22.875789835473118
50-51	22.870341470500914	27.159383852850777	27.079328679201097	22.89094599744721
52-53	23.029556120654533	27.032676390205097	27.010909997246515	22.926857491893855
54-55	22.989004808407426	26.941129339845617	27.093482803906504	22.976383047840454
56-57	22.928350544964655	27.074749031798216	27.081254506809955	22.91564591642717
58-59	22.92659827939551	27.07899785063287	27.07497014767874	22.91943372229288
60-61	23.027233516397818	27.055390213478027	27.054917373580988	22.86245889654317
62-63	23.004835628651726	27.210591097795668	26.9849296327538	22.799643640798802
64-65	22.978163511548242	27.130651641404967	26.930415172712102	22.96076967433469
66-67	23.005846658669366	27.182007783348837	26.849071649078766	22.963073908903027
68-69	22.925311816944447	27.10252161855423	26.928192937658647	23.04397362684267
70-71	22.987993116434488	26.895114645661767	26.99105211447559	23.12584012342816
72-73	23.027305874332516	26.952119035577542	26.839587627695426	23.180987462394516
74-75	22.857597562652973	27.096811509070466	26.920993181865814	23.124597746410743
76-77	23.084954804948815	27.013430110742114	26.84569465835845	23.055920425950628
78-79	22.916950313618237	27.06233028018107	26.812799629717254	23.207919776483447
80-81	23.019598154657693	27.099176230033105	26.772843626539515	23.10838198876969
82-83	23.013231242271747	27.034265269835238	26.93668880534909	23.015814682543926
84-85	23.01236326813423	27.073912631062512	26.877339117641704	23.036384983161557
86-87	22.923343921985463	27.09457465190171	26.952056294408454	23.030025131704374
88-89	22.94434606436941	27.05537923931507	26.899498065650164	23.10077663066536
90-91	22.99973848585938	27.08196616317375	26.916901120076425	23.001394230890448
92-93	23.053280942245085	27.054431178136433	26.8867376173737	23.00555026224477
94-95	22.84613517282619	27.09588801837819	27.03309060589766	23.024886202897967
96-97	22.965758229762294	27.185016290617913	27.018235930488427	22.830989549131367
98-99	24.07427293397325	29.150813322900365	27.14995283285624	19.624960910270147
100	23.00037097831053	30.096080477882854	20.94655520714068	25.95699333666594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8860.0
1	8871.5
2	10496.0
3	12111.5
4	15510.5
5	27589.0
6	37690.5
7	43081.0
8	44666.5
9	39756.0
10	31676.5
11	23150.5
12	22942.5
13	32809.0
14	60573.0
15	101334.5
16	133728.5
17	149387.5
18	150307.5
19	140443.0
20	123421.5
21	105078.5
22	94087.5
23	97612.0
24	114629.5
25	144011.0
26	182794.5
27	223925.0
28	267573.5
29	316832.0
30	363790.0
31	410315.5
32	460612.5
33	507923.5
34	556374.0
35	603444.5
36	653382.5
37	704452.5
38	749017.5
39	788409.0
40	824979.5
41	860788.0
42	891879.5
43	920760.0
44	952984.0
45	991873.5
46	1018602.5
47	1034865.5
48	1048489.5
49	1051671.0
50	1050419.5
51	1042257.5
52	1016555.5
53	970307.0
54	922275.5
55	871961.5
56	817022.0
57	756105.0
58	683055.5
59	601667.5
60	508926.5
61	419416.5
62	339396.0
63	266212.5
64	203497.0
65	154249.5
66	118234.5
67	89889.0
68	67959.0
69	51948.5
70	38862.5
71	28503.0
72	20821.0
73	14908.0
74	10464.0
75	7258.5
76	5077.0
77	3412.5
78	2371.5
79	1710.5
80	1189.5
81	817.0
82	600.5
83	413.5
84	295.5
85	225.0
86	167.5
87	124.5
88	90.5
89	66.5
90	46.0
91	32.5
92	25.5
93	14.0
94	6.5
95	5.5
96	5.0
97	4.5
98	2.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	9.24948585928548E-5
3	0.0
4	0.0
5	0.030390593321139293
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.1923397156298053E-4
24-25	9.254733046521108E-5
26-27	1.32839807952282E-4
28-29	1.2689072211294404E-4
30-31	2.017007447134386E-6
32-33	1.213177804208429E-5
34-35	1.8254117023412284E-5
36-37	5.804473913339856E-4
38-39	4.302089090365054E-5
40-41	0.0
42-43	6.233072792275743E-6
44-45	3.143474131984629E-5
46-47	6.3514385606082E-6
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	9.974884632340136E-5
70-71	9.136224123665681E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	5.134510306861955E-6
90-91	0.0
92-93	0.0
94-95	0.0012582298517196154
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	977.0
20-21	4344.0
22-23	6983.0
24-25	9036.0
26-27	14564.0
28-29	27379.0
30-31	57778.0
32-33	71682.0
34-35	93434.0
36-37	133113.0
38-39	166725.0
40-41	169845.0
42-43	194306.0
44-45	238866.0
46-47	232138.0
48-49	206972.0
50-51	176746.0
52-53	158737.0
54-55	169225.0
56-57	180609.0
58-59	190529.0
60-61	194130.0
62-63	178668.0
64-65	184595.0
66-67	199852.0
68-69	206694.0
70-71	212159.0
72-73	192764.0
74-75	190200.0
76-77	198656.0
78-79	217201.0
80-81	224058.0
82-83	213799.0
84-85	203925.0
86-87	212558.0
88-89	233689.0
90-91	254167.0
92-93	258653.0
94-95	298539.0
96-97	809822.0
98-99	1496591.0
100-101	1.6181539E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.08451566428947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.32885777151976	67.40947046940452
2	8.714609185980942	13.609520749817793
3	2.4319099572583123	5.696835334550347
4	1.015870423317504	3.1729499992969603
5	0.5201434975704156	2.0307576541857713
6	0.3052234452265286	1.4299934933939562
7	0.17818951924401427	0.9739689614635011
8	0.12699383396124908	0.7933001613772264
9	0.08602120303870507	0.6045231578523111
>10	0.2822341512681997	3.390096892257589
>50	0.006259909384002713	0.3390950194839487
>100	0.0035938907095666565	0.4920385017306928
>500	6.66105807149686E-5	0.03404045671681038
>1k	2.6600940061765115E-5	0.0234091484685909
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.2279702682917934E-5	0.0	0.0	4.021515590993687E-6
3	8.043031181987374E-6	6.836576504689269E-5	0.0	0.0	4.021515590993687E-6
4	8.043031181987374E-6	1.7694668600372223E-4	0.0	0.0	4.021515590993687E-6
5	8.043031181987374E-6	1.890112327767033E-4	0.0	0.0	4.021515590993687E-6
6	2.815060913695581E-5	1.890112327767033E-4	0.0	8.043031181987374E-6	2.0107577954968435E-5
7	2.815060913695581E-5	1.9303274836769699E-4	0.0	8.043031181987374E-6	2.0107577954968435E-5
8	2.815060913695581E-5	2.0107577954968435E-4	0.0	8.043031181987374E-6	2.4129093545962123E-5
9	2.815060913695581E-5	3.820439811444003E-4	0.0	1.6086062363974748E-5	3.2172124727949495E-5
10-11	2.815060913695581E-5	3.820439811444003E-4	0.0	1.809682015947159E-5	3.2172124727949495E-5
12-13	3.0161366932452655E-5	3.9008701232638764E-4	0.0	2.0107577954968435E-5	3.2172124727949495E-5
14-15	3.2172124727949495E-5	4.88614144305733E-4	0.0	2.0107577954968435E-5	5.429046047841478E-5
16-17	3.820439811444003E-5	5.107324800561983E-4	0.0	2.0107577954968435E-5	7.238728063788637E-5
18-19	4.021515590993687E-5	5.449153625796446E-4	0.0	2.0107577954968435E-5	8.445182741086744E-5
20-21	4.021515590993687E-5	5.811090028985878E-4	0.0	2.0107577954968435E-5	8.445182741086744E-5
22-23	4.021515590993687E-5	6.353994633770026E-4	0.0	3.619364031894318E-5	1.0455940536583587E-4
24-25	4.222591370543371E-5	7.077867440148889E-4	0.0	7.037652284238953E-5	1.3472077229828854E-4
26-27	4.423667150093056E-5	7.439803843338321E-4	0.0	1.9102199057220015E-4	1.3673153009378537E-4
28-29	4.423667150093056E-5	7.480018999248258E-4	0.0	5.52958393761632E-4	1.3673153009378537E-4
30-31	4.423667150093056E-5	8.445182741086743E-4	0.0	0.002095209622907711	1.387422878892822E-4
32-33	4.423667150093056E-5	8.525613052906616E-4	0.0	0.005336551189248623	1.4477456127577273E-4
34-35	4.423667150093056E-5	9.973358665664343E-4	0.0	0.009341980717878335	1.5281759245776012E-4
36-37	4.423667150093056E-5	0.0011441211856377039	0.0	0.016331374815025362	1.5281759245776012E-4
38-39	4.423667150093056E-5	0.0011863470993431378	0.0	0.035499928879496774	1.5482835025325696E-4
40-41	4.423667150093056E-5	0.0013311216606189105	0.0	0.05700096198674452	1.5884986584425065E-4
42-43	4.423667150093056E-5	0.001377369089915338	0.0	0.0693791869758231	1.6086062363974748E-4
44-45	4.423667150093056E-5	0.0015502942603280664	0.0	0.08323934046018283	1.6488213923074118E-4
46-47	4.423667150093056E-5	0.0016809935170353611	0.0	0.09770674279878262	1.6890365482173487E-4
48-49	4.423667150093056E-5	0.0016890365482173486	0.0	0.11089932469503741	1.890112327767033E-4
50-51	4.423667150093056E-5	0.0019343489992679635	0.0	0.12376013155503522	1.890112327767033E-4
52-53	4.423667150093056E-5	0.001954456577222932	0.0	0.15324186235260995	2.0509729514067804E-4
54-55	4.423667150093056E-5	0.001998693248723863	0.0	0.18412308057585047	2.2520487309564646E-4
56-57	4.423667150093056E-5	0.0020389084046337994	0.0	0.2135284025771963	2.352586620731307E-4
58-59	4.423667150093056E-5	0.0020489621936112838	0.0	0.23502943568444407	2.4129093545962124E-4
60-61	4.6247429296427406E-5	0.002085155833930227	0.0	0.2456381938134854	2.4330169325511807E-4
62-63	4.8258187091924247E-5	0.0021253709898401635	0.0	0.25854926961837066	2.533554822326023E-4
64-65	4.8258187091924247E-5	0.002370683440890779	0.0	0.2646961561992045	2.553662400280991E-4
66-67	4.8258187091924247E-5	0.00238878026105025	0.0	0.2699784169279747	2.6139851341458963E-4
68-69	4.8258187091924247E-5	0.002443070721528665	0.0	0.2752325270476079	2.6542002900558335E-4
70-71	4.8258187091924247E-5	0.002487307393029595	0.0	0.2798291193681137	2.714523023920739E-4
72-73	4.8258187091924247E-5	0.0025114364865755578	0.0	0.28361537629703426	3.136782160975076E-4
74-75	4.8258187091924247E-5	0.0025838237672134437	0.0	0.28644049099970736	3.1568897389300444E-4
76-77	4.8258187091924247E-5	0.002724576812898223	0.0	0.2892535411556074	3.176997316885013E-4
78-79	4.8258187091924247E-5	0.0027828887889676315	0.0	0.2911597395457384	3.176997316885013E-4
80-81	4.8258187091924247E-5	0.0027969640935361093	0.0	0.29187556932093534	3.337857940524761E-4
82-83	4.8258187091924247E-5	0.0028110393981045875	0.0	0.2923139145203536	3.619364031894318E-4
84-85	4.8258187091924247E-5	0.0028512545540145245	0.0	0.29249287196415286	3.659579187804255E-4
86-87	4.8258187091924247E-5	0.002905545014492939	0.0	0.2925632484869952	3.6997943437141925E-4
88	4.8258187091924247E-5	0.0030362442712002337	0.0	0.2925733022759727	3.8606549673539397E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	33830	0.0	50.385193	1
GTATCAA	60535	0.0	36.754715	1
TCAACGC	76035	0.0	28.591116	4
ATCAACG	76890	0.0	28.26213	3
CAACGCA	77920	0.0	27.883085	5
TATCAAC	79240	0.0	27.76737	2
AACGCAG	79980	0.0	27.241152	6
ACGCAGA	92755	0.0	23.448029	7
CGCAGAG	95590	0.0	22.770395	8
GTACATG	79365	0.0	22.211054	1
TACATGG	80920	0.0	21.369144	2
ACATGGG	81525	0.0	20.673391	3
GCAGAGT	109625	0.0	19.727215	9
CATGGGG	59760	0.0	18.718847	4
AGAGTAC	98600	0.0	18.665483	10-11
GAGTACT	63850	0.0	18.316263	12-13
CAGAGTA	106510	0.0	17.788086	10-11
GTGGTAT	15085	0.0	17.221252	1
AGTACTT	70010	0.0	16.677343	12-13
GTACTTT	72290	0.0	16.116941	14-15
>>END_MODULE