FastQCFastQC Report
Fri 27 May 2016
SRR2140258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24963512
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA375900.054.5965271
GTATCAA710600.038.464181
TCAACGC907700.029.4897924
ATCAACG917650.029.21633
TATCAAC928550.029.0563432
CAACGCA925950.028.8764695
AACGCAG949150.028.230166
ACGCAGA1081750.024.750117
CGCAGAG1165600.022.9660158
GTACATG942200.022.2279131
TACATGG958750.021.5652392
ACATGGG962350.020.8845043
GCAGAGT1278850.020.826039
TATATAC302550.020.0663243
ATACAAC233000.020.0559736
GAGTACT717250.019.44802512-13
GTGGTAT153450.019.256311
AGAGTAC1153900.019.16565310-11
TATACAA280650.018.8335975
CAGAGTA1233350.018.78448910-11
Produced by FastQC (version 0.11.5)