##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140258_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24963512
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34326259862795	33.0	31.0	34.0	31.0	34.0
2	32.3862516219673	34.0	31.0	34.0	30.0	34.0
3	32.4717506094495	34.0	31.0	34.0	31.0	34.0
4	36.04622242255016	37.0	35.0	37.0	35.0	37.0
5	35.9771706801511	37.0	35.0	37.0	35.0	37.0
6	36.057371775253415	37.0	35.0	37.0	35.0	37.0
7	35.99715933399115	37.0	35.0	37.0	35.0	37.0
8	35.99990349915509	37.0	35.0	37.0	35.0	37.0
9	37.694114634190896	39.0	37.0	39.0	35.0	39.0
10-11	37.631550140060426	39.0	37.0	39.0	35.0	39.0
12-13	37.61925393350102	39.0	37.0	39.0	35.0	39.0
14-15	38.995010537780104	40.0	38.0	41.0	35.0	41.0
16-17	38.992623173374	40.0	38.0	41.0	35.0	41.0
18-19	39.02790799227288	40.0	38.5	41.0	35.0	41.0
20-21	39.0395802733209	40.0	39.0	41.0	35.0	41.0
22-23	38.99816859220872	40.0	38.5	41.0	35.0	41.0
24-25	38.96023444281059	40.0	38.0	41.0	35.0	41.0
26-27	38.85617605658407	40.0	38.0	41.0	35.0	41.0
28-29	38.7988065499342	40.0	38.0	41.0	35.0	41.0
30-31	38.69444843873981	40.0	38.0	41.0	34.5	41.0
32-33	38.59107809492406	40.0	38.0	41.0	34.0	41.0
34-35	38.53766912121719	40.0	38.0	41.0	34.0	41.0
36-37	38.46561535379825	40.0	38.0	41.0	34.0	41.0
38-39	38.40740111241627	40.0	38.0	41.0	34.0	41.0
40-41	38.34605960074663	40.0	38.0	41.0	34.0	41.0
42-43	38.27189556174857	40.0	38.0	41.0	33.0	41.0
44-45	38.21141584150169	40.0	38.0	41.0	33.0	41.0
46-47	38.12800965242061	40.0	37.0	41.0	33.0	41.0
48-49	38.02736463980247	40.0	37.0	41.0	33.0	41.0
50-51	37.9093096722099	40.0	37.0	41.0	33.0	41.0
52-53	37.80073039116461	40.0	37.0	41.0	33.0	41.0
54-55	37.648489917337436	40.0	36.0	41.0	32.5	41.0
56-57	37.48132456164582	39.0	36.0	41.0	32.0	41.0
58-59	37.28674669109799	39.0	35.0	41.0	32.0	41.0
60-61	37.07216056143747	39.0	35.0	40.5	32.0	41.0
62-63	36.80956579223485	38.0	35.0	40.0	31.0	41.0
64-65	36.54489248540914	38.0	35.0	40.0	31.0	41.0
66-67	36.25855842834697	37.0	35.0	40.0	31.0	41.0
68-69	35.94840903710015	37.0	34.5	39.5	31.0	41.0
70-71	35.62930699404407	36.0	34.0	39.0	30.0	41.0
72-73	35.311448302794204	36.0	34.0	39.0	30.0	40.0
74-75	35.005187043024755	35.0	34.0	38.0	30.0	40.0
76-77	34.68545039622687	35.0	34.0	37.0	30.0	39.0
78-79	34.3847123356632	35.0	34.0	37.0	29.5	39.0
80-81	34.12511760149869	35.0	33.5	36.0	29.0	39.0
82-83	33.8568758545905	35.0	33.0	36.0	29.0	37.0
84-85	33.65156120808166	35.0	33.0	35.5	29.0	37.0
86-87	33.46534983806791	35.0	33.0	35.0	29.0	36.5
88-89	33.34234840076589	35.0	33.0	35.0	29.0	36.0
90-91	33.22231896957623	35.0	33.0	35.0	29.0	36.0
92-93	33.09848789777753	35.0	33.0	35.0	29.0	36.0
94-95	33.34233149086823	35.0	33.0	35.0	29.5	35.5
96-97	33.41656522486822	35.0	33.0	35.0	30.0	35.0
98-99	33.75766793602779	35.0	34.0	35.0	31.0	35.0
100	33.56719428274895	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	23.0
20	130.0
21	732.0
22	3456.0
23	11571.0
24	30855.0
25	66132.0
26	118482.0
27	187695.0
28	268931.0
29	358384.0
30	457169.0
31	573009.0
32	718711.0
33	922741.0
34	1258600.0
35	1799230.0
36	2945362.0
37	5317354.0
38	6443943.0
39	3324212.0
40	156790.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.01327120959583	19.86260186467353	13.663422037732511	24.46070488799813
2	15.104016697663933	21.66693666094471	40.048451554774466	23.18059508661689
3	17.789355920753458	26.963469723330597	30.54749668235783	24.699677673558114
4	12.601676398737485	18.420060446623054	38.28247403650576	30.6957891181337
5	13.115709952053239	38.08117990984463	35.32764020234971	13.475469935752423
6	29.601584104031513	38.16585983574747	18.743364315085152	13.489191745135862
7	26.099644953802976	32.809818586423255	23.615751661865524	17.474784797908242
8	25.087928333160814	35.967643495033876	20.88081997436899	18.063608197436324
9	26.265839518093447	15.827212132651846	20.765026972166417	37.14192137708829
10-11	24.53513151514899	26.416865944182856	28.686678781415047	20.361323759253107
12-13	25.62212400242402	24.301450452965113	28.57191528179208	21.50451026281879
14-15	22.74715793194483	25.052094032281996	26.44256545313015	25.75818258264302
16-17	22.065697326562063	28.154087453720457	26.926577878945878	22.853637340771606
18-19	22.254961561498238	27.197190843980607	28.680053111116738	21.867794483404417
20-21	23.337172469040322	26.21028471064856	28.600430622309545	21.85211219800157
22-23	23.098750620630618	26.186552978584192	28.39118359322518	22.32351280756001
24-25	22.736310670894966	26.38609968351894	28.565165599438224	22.31242404614788
26-27	22.572180140548863	26.405948240280242	28.566266520920962	22.455605098249933
28-29	22.691478383118536	26.387990423229375	28.52931550302192	22.391215690630176
30-31	23.023512685734087	26.14002516979	28.417994033074496	22.418468111401417
32-33	22.822188096329004	26.361865153490992	28.311146915326944	22.504799834853053
34-35	22.960327323044037	26.38148019194381	28.26752095338786	22.390671531624296
36-37	23.113410378298298	26.253256608871787	28.102494396971395	22.530838615858517
38-39	22.818262824113482	26.53998594854723	28.118163686957885	22.5235875403814
40-41	22.94009790740043	26.324135628385935	28.112832709466	22.622933754747635
42-43	22.778835786969452	26.563667557298597	27.78074103143167	22.87675562430028
44-45	22.850033997951247	26.60061738316135	27.596388186320258	22.952960432567142
46-47	23.05820138726116	26.659012319892582	27.261266997687926	23.02151929515833
48-49	23.217283087514257	26.898829277083685	26.924499116172022	22.959388519230036
50-51	23.165432640231014	26.943382700629247	26.884097716815	23.00708694232474
52-53	23.39055981022283	26.78067213095277	26.797459491143737	23.03130856768066
54-55	23.36379341005402	26.757023562192632	26.800472329798797	23.078710697954556
56-57	23.304405457315575	26.98710796134451	26.69571891458158	23.012767666758336
58-59	23.19570102254343	26.903110101261817	26.859329677906942	23.04185919828781
60-61	23.298263005186058	26.8860456674304	26.73439400619873	23.081297321184813
62-63	23.290019127343488	26.92166088621379	26.864138198584403	22.924181787858323
64-65	23.444793880261336	26.766331895965827	26.74456790070629	23.04430632306655
66-67	23.411953001391275	26.930569752916107	26.57868259483106	23.078794650861557
68-69	23.34764082334003	26.809649822351833	26.651844852095312	23.190864502212825
70-71	23.37749457483084	26.67021463118967	26.674425402271922	23.277865391707564
72-73	23.349139051181254	26.78910104811458	26.502790841351626	23.358969059352535
74-75	23.15808333836394	26.85857188441578	26.622650898022393	23.360693879197882
76-77	23.35950325471004	26.874332255465365	26.543570887852397	23.222593601972196
78-79	23.165856208075887	26.914553273291343	26.569772573665457	23.34981794496732
80-81	23.292569117222197	26.830802658256413	26.634780046958724	23.241848177562662
82-83	23.38053014237914	26.723380170281974	26.822165574403474	23.073924112935412
84-85	23.282984367457637	26.774284931262798	26.759022146536378	23.18370855474319
86-87	23.186133163772656	26.981718276217215	26.644335298034598	23.187813261975535
88-89	23.371430403173925	26.774781055640045	26.525803521143885	23.327985020042146
90-91	23.392144255871035	26.82463850536393	26.59148947000123	23.19172776876381
92-93	23.415661019041657	26.86862011876433	26.674042926128443	23.04167593606557
94-95	23.06273198975716	27.00196755172504	26.75965784514098	23.17564261337682
96-97	23.19712402343352	27.0735072294645	26.755298877354306	22.974069869747673
98-99	24.410182575933188	29.00837263989003	26.835483111822484	19.745961672354298
100	23.231179085354945	29.99814697490109	20.767949801815053	26.00272413792891
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5971.0
1	6105.5
2	9564.0
3	12945.0
4	16099.5
5	27536.0
6	37518.0
7	43696.5
8	46601.5
9	42828.5
10	34864.5
11	25904.0
12	25514.0
13	35861.0
14	66159.5
15	111142.0
16	147578.0
17	167086.0
18	170058.0
19	158110.5
20	136707.0
21	113422.0
22	98063.5
23	99252.0
24	115306.0
25	144994.0
26	184294.5
27	224417.0
28	267207.0
29	315946.5
30	360359.5
31	402380.0
32	449817.5
33	496905.0
34	542191.5
35	584807.5
36	632950.0
37	679340.5
38	713138.0
39	746114.5
40	782206.5
41	812855.0
42	832466.0
43	854638.0
44	887574.0
45	933670.5
46	978390.0
47	1028280.0
48	1065125.0
49	1071330.0
50	1073610.0
51	1080959.5
52	1046034.0
53	978136.5
54	935397.0
55	897421.5
56	851397.0
57	790863.0
58	720572.5
59	637379.5
60	540446.5
61	459397.5
62	379285.0
63	304242.0
64	249084.5
65	192424.0
66	147213.0
67	110161.5
68	76263.0
69	56589.5
70	42997.5
71	31117.5
72	22888.5
73	16836.5
74	12307.5
75	8723.0
76	5852.0
77	3805.5
78	2724.0
79	2160.0
80	1627.0
81	1191.0
82	928.0
83	582.5
84	319.0
85	204.0
86	140.5
87	102.0
88	66.5
89	46.5
90	30.5
91	22.5
92	18.5
93	8.5
94	1.0
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.011693226497938E-5
3	0.0
4	0.0
5	0.030873059848309806
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	4.006124162006459E-6
22-23	1.9834495758332978E-4
24-25	8.016231265065505E-5
26-27	1.5838702820664463E-4
28-29	1.1837228557301856E-4
30-31	2.009146963012629E-6
32-33	2.8195812019649096E-5
34-35	1.818146777534833E-5
36-37	5.497211970282518E-4
38-39	5.917170870836975E-5
40-41	0.0
42-43	2.069792963370398E-6
44-45	3.341834054086874E-5
46-47	4.222155910917999E-6
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	9.267100685149189E-5
70-71	8.421535072266478E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	5.1247868953459325E-6
90-91	0.0
92-93	0.0
94-95	0.0011676412072098485
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1074.0
20-21	4206.0
22-23	6915.0
24-25	9467.0
26-27	14496.0
28-29	27694.0
30-31	56507.0
32-33	71368.0
34-35	93021.0
36-37	131776.0
38-39	171789.0
40-41	171080.0
42-43	204310.0
44-45	252346.0
46-47	243434.0
48-49	213555.0
50-51	185352.0
52-53	169373.0
54-55	175601.0
56-57	184489.0
58-59	192418.0
60-61	192397.0
62-63	178575.0
64-65	184424.0
66-67	195603.0
68-69	205296.0
70-71	208772.0
72-73	191379.0
74-75	190265.0
76-77	198214.0
78-79	213936.0
80-81	223274.0
82-83	212440.0
84-85	205528.0
86-87	213463.0
88-89	233783.0
90-91	252475.0
92-93	258023.0
94-95	297465.0
96-97	803509.0
98-99	1496901.0
100-101	1.6227519E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.11519058482351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.72444014393557	68.61220610349582
2	8.62982189636675	13.65500008088277
3	2.348008249695888	5.572893605082842
4	0.9749880249747584	3.0854545365519472
5	0.4569552991839911	1.8076052791843253
6	0.27509989529205847	1.3058748387537722
7	0.15792363861344896	0.8745911136726267
8	0.1064632509172787	0.6738288309280314
9	0.06702226984261832	0.47722316868235964
>10	0.25013049428516243	3.1045412188742896
>50	0.005892955077471638	0.3198647468412857
>100	0.003165850479890328	0.44852114464625953
>500	6.293720850061887E-5	0.03159299069740041
>1k	2.5094126598186443E-5	0.030802341706156644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0058466132489695E-6
2	0.0	6.809939242523247E-5	0.0	0.0	4.0058466132489695E-6
3	0.0	6.809939242523247E-5	0.0	0.0	4.0058466132489695E-6
4	0.0	1.161695517842201E-4	0.0	0.0	8.011693226497939E-6
5	0.0	1.161695517842201E-4	0.0	4.0058466132489695E-6	8.011693226497939E-6
6	0.0	1.161695517842201E-4	0.0	4.0058466132489695E-6	1.2017539839746907E-5
7	0.0	1.161695517842201E-4	0.0	4.0058466132489695E-6	1.2017539839746907E-5
8	0.0	1.3619878485046495E-4	0.0	1.2017539839746907E-5	1.2017539839746907E-5
9	4.0058466132489695E-6	2.6038002986118297E-4	0.0	1.2017539839746907E-5	1.6023386452995878E-5
10-11	4.0058466132489695E-6	2.6038002986118297E-4	0.0	1.6023386452995878E-5	1.6023386452995878E-5
12-13	4.0058466132489695E-6	2.6038002986118297E-4	0.0	1.6023386452995878E-5	2.2032156372869333E-5
14-15	8.011693226497939E-6	3.605261951924072E-4	0.0	1.6023386452995878E-5	3.004384959936727E-5
16-17	1.6023386452995878E-5	3.645320418056562E-4	0.0	1.6023386452995878E-5	3.6052619519240724E-5
18-19	1.6023386452995878E-5	3.9457589140502345E-4	0.0	2.60380029861183E-5	3.8055542825865204E-5
20-21	2.4035079679493814E-5	4.3062851092426417E-4	0.0	3.404969621261624E-5	4.406431274573866E-5
22-23	2.4035079679493814E-5	4.887132868163742E-4	0.0	3.6052619519240724E-5	5.808477589211005E-5
24-25	2.4035079679493814E-5	5.608185258548557E-4	0.0	3.8055542825865204E-5	6.6096469118608E-5
26-27	2.4035079679493814E-5	5.968711453740965E-4	0.0	6.209062250535902E-5	7.210523903848143E-5
28-29	2.8040926292742785E-5	5.968711453740965E-4	0.0	2.0429817727569743E-4	8.211985557160387E-5
30-31	3.8055542825865204E-5	6.409354581198351E-4	0.0	7.791371662769245E-4	8.412277887822836E-5
32-33	4.606723605236314E-5	6.429383814264596E-4	0.0	0.0020109349998509826	8.412277887822836E-5
34-35	4.807015935898763E-5	7.771342429703E-4	0.0	0.003547177176031962	8.412277887822836E-5
36-37	4.807015935898763E-5	9.113301045141405E-4	0.0	0.00582850682227725	8.412277887822836E-5
38-39	4.807015935898763E-5	9.794294969393729E-4	0.0	0.00986640020843221	9.01315487981018E-5
40-41	4.807015935898763E-5	0.0010976019720302175	0.0	0.014354951338577681	9.614031871797525E-5
42-43	4.807015935898763E-5	0.0011556867479223275	0.0	0.018342771642067032	9.614031871797525E-5
44-45	4.807015935898763E-5	0.0012918855327727925	0.0	0.022915445551090728	1.041520119444732E-4
46-47	4.807015935898763E-5	0.0013780112349576453	0.0	0.02797082397701093	1.0815785855772216E-4
48-49	4.807015935898763E-5	0.00138001415826427	0.0	0.03324652396665982	1.2418124501071805E-4
50-51	4.807015935898763E-5	0.0015522655626339756	0.0	0.038624373044946564	1.2418124501071805E-4
52-53	4.807015935898763E-5	0.001558274332553849	0.0	0.0439201022676617	1.3820170815708943E-4
54-55	4.807015935898763E-5	0.0015722947957002202	0.0	0.04872311235694721	1.5823094122333428E-4
56-57	5.0073082665612114E-5	0.0016143561851393346	0.0	0.05368835923406931	1.702484810630812E-4
58-59	5.20760059722366E-5	0.0016223678783658325	0.0	0.05877778735620212	1.7625725098295464E-4
60-61	5.20760059722366E-5	0.001662426344498322	0.0	0.06429984691256582	1.802630975962036E-4
62-63	5.20760059722366E-5	0.0016964760407109382	0.0	0.06948341243011	1.922806374359505E-4
64-65	5.20760059722366E-5	0.0019147946811330072	0.0	0.07428041374947564	1.922806374359505E-4
66-67	5.20760059722366E-5	0.0019288151442793788	0.0	0.07891117243439144	1.94283560742575E-4
68-69	5.20760059722366E-5	0.0020109349998509826	0.0	0.08369014744399747	1.9828940735582397E-4
70-71	5.20760059722366E-5	0.0020509934659834724	0.0	0.08812662256817069	2.103069471955709E-4
72-73	5.407892927886109E-5	0.0020630110058232195	0.0	0.09161571496831054	2.2833325695519125E-4
74-75	5.608185258548557E-5	0.00211708993510208	0.0	0.09426157665636149	2.303361802618157E-4
76-77	5.608185258548557E-5	0.002229253640273051	0.0	0.09674720447988247	2.323391035684402E-4
78-79	6.209062250535902E-5	0.0022753208763254145	0.0	0.09845369513712654	2.3634495018168917E-4
80-81	6.409354581198351E-5	0.002277323799632039	0.0	0.09913468906137887	2.523683366346851E-4
82-83	6.409354581198351E-5	0.0022813296462452877	0.0	0.09960537603843561	2.8040926292742784E-4
84-85	6.409354581198351E-5	0.0023013588793115328	0.0	0.0997836362127252	2.884209561539258E-4
86-87	6.409354581198351E-5	0.0023694582717367655	0.0	0.09984372391192392	2.9242680276717476E-4
88	6.409354581198351E-5	0.0025397067527998464	0.0	0.0998497326818438	3.0043849599367266E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	37590	0.0	54.596527	1
GTATCAA	71060	0.0	38.46418	1
TCAACGC	90770	0.0	29.489792	4
ATCAACG	91765	0.0	29.2163	3
TATCAAC	92855	0.0	29.056343	2
CAACGCA	92595	0.0	28.876469	5
AACGCAG	94915	0.0	28.23016	6
ACGCAGA	108175	0.0	24.75011	7
CGCAGAG	116560	0.0	22.966015	8
GTACATG	94220	0.0	22.227913	1
TACATGG	95875	0.0	21.565239	2
ACATGGG	96235	0.0	20.884504	3
GCAGAGT	127885	0.0	20.82603	9
TATATAC	30255	0.0	20.066324	3
ATACAAC	23300	0.0	20.055973	6
GAGTACT	71725	0.0	19.448025	12-13
GTGGTAT	15345	0.0	19.25631	1
AGAGTAC	115390	0.0	19.165653	10-11
TATACAA	28065	0.0	18.833597	5
CAGAGTA	123335	0.0	18.784489	10-11
>>END_MODULE