FastQCFastQC Report
Fri 27 May 2016
SRR2140257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23182519
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA391850.050.1811451
GTATCAA707150.037.1294751
TCAACGC889900.028.9141834
ATCAACG892200.028.8301453
CAACGCA901900.028.5153775
TATCAAC919150.028.2706242
AACGCAG930450.027.7463476
ACGCAGA1048850.024.5414947
CGCAGAG1067300.024.1251938
GTACATG828700.021.043931
GCAGAGT1221950.020.995659
TACATGG841700.020.426952
ACATGGG837100.020.1191023
GAGTACT728450.019.2930912-13
AGAGTAC1118100.019.06083910-11
CAGAGTA1195000.018.67252310-11
CATGGGG585600.018.1602364
AGTACTT782100.017.50929312-13
GTACTTT815500.017.20858414-15
ACTTTTT919150.015.46260816-17
Produced by FastQC (version 0.11.5)