##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23182519
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40315666300112	34.0	31.0	34.0	31.0	34.0
2	32.44718626133769	34.0	31.0	34.0	31.0	34.0
3	32.53080487068726	34.0	31.0	34.0	31.0	34.0
4	36.087301686240394	37.0	35.0	37.0	35.0	37.0
5	36.02149408353769	37.0	35.0	37.0	35.0	37.0
6	36.098089297371004	37.0	35.0	37.0	35.0	37.0
7	36.03830317145432	37.0	35.0	37.0	35.0	37.0
8	36.03941564762656	37.0	35.0	37.0	35.0	37.0
9	37.73887617648453	39.0	38.0	39.0	35.0	39.0
10-11	37.67763373773144	39.0	37.5	39.0	35.0	39.0
12-13	37.665263813652004	39.0	37.0	39.0	35.0	39.0
14-15	39.046677563383	40.0	38.0	41.0	36.0	41.0
16-17	39.04481150214953	40.0	38.0	41.0	36.0	41.0
18-19	39.08413030956645	40.0	38.5	41.0	36.0	41.0
20-21	39.09855429165789	40.0	39.0	41.0	35.0	41.0
22-23	39.05770286711769	40.0	39.0	41.0	35.0	41.0
24-25	39.02024152236969	40.0	38.5	41.0	35.0	41.0
26-27	38.91635698434888	40.0	38.0	41.0	35.0	41.0
28-29	38.85995136026716	40.0	38.0	41.0	35.0	41.0
30-31	38.75524989645309	40.0	38.0	41.0	35.0	41.0
32-33	38.651411104129565	40.0	38.0	41.0	34.0	41.0
34-35	38.60052002503518	40.0	38.0	41.0	34.0	41.0
36-37	38.53553401492264	40.0	38.0	41.0	34.0	41.0
38-39	38.48590354317834	40.0	38.0	41.0	34.0	41.0
40-41	38.43756313947763	40.0	38.0	41.0	34.0	41.0
42-43	38.372907994662526	40.0	38.0	41.0	34.0	41.0
44-45	38.32240145554875	40.0	38.0	41.0	33.5	41.0
46-47	38.24985059212757	40.0	38.0	41.0	33.0	41.0
48-49	38.16348400499857	40.0	37.5	41.0	33.0	41.0
50-51	38.06450888742884	40.0	37.0	41.0	33.0	41.0
52-53	37.97085353894667	40.0	37.0	41.0	33.0	41.0
54-55	37.83487742101873	40.0	37.0	41.0	33.0	41.0
56-57	37.68233397602657	39.5	36.0	41.0	33.0	41.0
58-59	37.50607778555856	39.0	36.0	41.0	32.0	41.0
60-61	37.312168801262494	39.0	35.5	41.0	32.0	41.0
62-63	37.06721961161011	39.0	35.0	40.5	32.0	41.0
64-65	36.81888632959759	38.0	35.0	40.0	31.0	41.0
66-67	36.56231935761994	37.5	35.0	40.0	31.0	41.0
68-69	36.253397883078875	37.0	35.0	40.0	31.0	41.0
70-71	35.94196240446893	36.5	34.5	39.0	31.0	41.0
72-73	35.62402581290806	36.0	34.0	39.0	30.5	41.0
74-75	35.311061478607826	36.0	34.0	39.0	30.0	40.0
76-77	34.98483092696134	35.0	34.0	37.5	30.0	40.0
78-79	34.66238719073773	35.0	34.0	37.0	30.0	39.0
80-81	34.378932213278524	35.0	34.0	37.0	30.0	39.0
82-83	34.0931080651996	35.0	34.0	36.0	29.5	38.5
84-85	33.86682832509295	35.0	33.0	36.0	29.0	37.0
86-87	33.65531770463106	35.0	33.0	35.5	29.0	37.0
88-89	33.514801423800485	35.0	33.0	35.0	29.0	36.5
90-91	33.378600224128355	35.0	33.0	35.0	29.0	36.0
92-93	33.25329380887019	35.0	33.0	35.0	29.0	36.0
94-95	33.474016181471576	35.0	33.0	35.0	30.0	36.0
96-97	33.52476476734145	35.0	34.0	35.0	30.0	35.5
98-99	33.84255379965766	35.0	34.0	35.0	31.0	35.0
100	33.65533187072199	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	5.0
19	22.0
20	92.0
21	652.0
22	2887.0
23	10047.0
24	26430.0
25	56689.0
26	102142.0
27	162926.0
28	234299.0
29	314201.0
30	403160.0
31	504474.0
32	633458.0
33	816618.0
34	1112069.0
35	1576934.0
36	2525628.0
37	4707942.0
38	6249574.0
39	3570323.0
40	171946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.591601844476	20.421663409399123	13.963586096920702	24.02314864920417
2	14.596599718882716	21.593754252939558	41.110803647320964	22.698842380856764
3	17.351009180667553	27.663016258069277	30.90465276875218	24.081321792510987
4	12.125015404926446	18.629248184806837	39.50465434752798	29.741082062738737
5	12.439952560095335	38.67921564850631	35.92617456093364	12.954657230464717
6	28.678328701035465	39.18494793426029	19.06858784414239	13.068135520561853
7	25.301508434005815	33.29934939339422	24.27240542755513	17.12673674504483
8	24.39923806381869	36.67369365684549	21.314318776143352	17.61274950319247
9	25.909069674438744	16.06321124982147	21.263474430884756	36.76424464485503
10-11	23.96508334577446	26.725331272240087	29.388264493603995	19.92132088838146
12-13	25.175719687752657	24.56585283074717	29.3633232868266	20.895104194673582
14-15	22.27839865029335	25.501648461929438	27.160253810209323	25.05969907756789
16-17	21.452573812190128	28.516266933718466	27.7594660873566	22.271693166734817
18-19	21.534275459884235	27.652650689081714	29.70453512838704	21.10853872264701
20-21	22.521123562216033	26.713451705634956	29.521882718880033	21.24354201326898
22-23	22.275632154081563	26.63190628417704	29.38418962941843	21.70827193232297
24-25	21.913811917770317	26.861061692546322	29.46283124845987	21.76229514122349
26-27	21.895544240940364	26.917547405672366	29.38599432296458	21.80091403042269
28-29	21.88751769742964	26.880844904944308	29.468447316699137	21.76319008092692
30-31	22.165857179843915	26.82294228940134	29.264013808971352	21.747186721783397
32-33	21.92712339626682	27.067821480752595	29.196913317023498	21.808141805957085
34-35	22.06810692264613	27.05974259840238	29.206722229729543	21.665428249221947
36-37	22.13145365384768	26.977220829769603	29.048816742991075	21.84250877339164
38-39	22.031950307295727	27.10244368005074	29.087480909866848	21.778125102786678
40-41	22.221905708595436	26.932731716054615	28.972538391009262	21.87282418434069
42-43	22.1205264569345	27.13000062285358	28.6288057180282	22.12066720218372
44-45	22.18830710693297	27.299545435120177	28.40514852543519	22.10699893251167
46-47	22.378043657016633	27.334629409472626	27.9621637851104	22.325163148400335
48-49	22.39949005571068	27.458468094221505	27.754640315335006	22.38740153473281
50-51	22.388918682005258	27.58565850482596	27.580296906916264	22.44512590625252
52-53	22.508021033521135	27.52704233823591	27.500837426990977	22.464099201251983
54-55	22.471748193318366	27.41862607375006	27.581509861544646	22.528115871386927
56-57	22.469601320302505	27.52843346202672	27.571073090980292	22.430892126690477
58-59	22.44450158385847	27.557685150904383	27.598709831290652	22.399103433946497
60-61	22.56461636223491	27.605917155321997	27.49097878721148	22.33848769523161
62-63	22.525863295044545	27.629395109329735	27.504068948729387	22.340672646896326
64-65	22.464200872397203	27.5212124061469	27.511174496916873	22.50341222453902
66-67	22.54729433996863	27.596006799029084	27.367841898994378	22.48885696200791
68-69	22.421050849501896	27.54105510096832	27.480924499606207	22.556969549923576
70-71	22.468621716124964	27.29458346970422	27.57427190082745	22.662522913343363
72-73	22.535426032963375	27.40505872181074	27.44143978644052	22.618075458785366
74-75	22.362852374092654	27.631285102694868	27.466943843467128	22.538918679745347
76-77	22.55147951878375	27.499111761114552	27.43707668951873	22.512332030582968
78-79	22.447168750249475	27.465400367726435	27.408462893685986	22.678967988338105
80-81	22.511589207431562	27.56640329158209	27.359332106048367	22.562675394937976
82-83	22.561321226863477	27.454639550675353	27.52363891034049	22.46040031212068
84-85	22.57331890543731	27.556594196948364	27.350743920440934	22.51934297717339
86-87	22.397306312165036	27.639426864471094	27.49750229450651	22.465764528857363
88-89	22.510610508053375	27.516634015473716	27.41028686590986	22.56246861056305
90-91	22.52693219494032	27.563857563812483	27.44765451319258	22.46155572805462
92-93	22.648173987610587	27.532013946614757	27.38713623197319	22.43267583380146
94-95	22.354415571594973	27.530062261799422	27.554783634503032	22.560738532102572
96-97	22.426620146256262	27.679835533019066	27.576030336280922	22.317513984443746
98-99	23.52400383617691	29.63902507215624	27.713165116925143	19.12380597474171
100	22.52013521508634	30.543174128274003	21.53698300211542	25.399707654524235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5855.0
1	6096.5
2	9830.5
3	13489.0
4	17502.0
5	30988.5
6	42200.5
7	48075.0
8	49708.5
9	44412.5
10	35810.0
11	26482.5
12	26116.0
13	37342.5
14	69475.0
15	116420.5
16	154167.0
17	173698.5
18	175360.0
19	162514.0
20	140929.0
21	118224.5
22	104228.0
23	105727.0
24	122537.0
25	153511.0
26	195814.5
27	240781.5
28	287941.5
29	341939.0
30	395883.5
31	447466.5
32	500163.0
33	549614.5
34	599401.5
35	645168.5
36	693283.5
37	744805.5
38	779307.5
39	802229.5
40	824103.5
41	837380.0
42	841421.5
43	857013.5
44	880364.0
45	904862.5
46	920121.0
47	925505.5
48	933189.0
49	932414.0
50	920018.0
51	905425.5
52	883083.0
53	841545.0
54	795759.0
55	747650.5
56	696493.0
57	637200.5
58	571697.5
59	502558.5
60	420385.5
61	340614.5
62	272046.5
63	212377.0
64	161415.0
65	120270.5
66	88919.5
67	67101.5
68	51163.5
69	38311.0
70	28650.0
71	21139.5
72	15100.0
73	10542.5
74	7356.5
75	4957.5
76	3229.5
77	2092.0
78	1446.5
79	1042.5
80	753.5
81	523.5
82	370.5
83	276.5
84	215.0
85	173.5
86	134.0
87	91.0
88	53.5
89	30.0
90	20.0
91	17.5
92	15.5
93	11.0
94	5.5
95	2.5
96	2.0
97	2.0
98	1.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.0352628202310544E-4
3	0.0
4	0.0
5	0.03059632993291195
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.1569401284971198E-6
22-23	2.1789553600435723E-4
24-25	8.200354337310914E-5
26-27	1.489659335419792E-4
28-29	1.1666840661031093E-4
30-31	2.163872861563464E-6
32-33	1.301720303654031E-5
34-35	1.52371469278244E-5
36-37	5.81577009027715E-4
38-39	5.0602646720834086E-5
40-41	0.0
42-43	8.936205501271086E-6
44-45	2.9317300648782844E-5
46-47	1.368483969282279E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	9.266264843022336E-5
70-71	7.332669060943254E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	1.10660115809131E-5
90-91	0.0
92-93	0.0
94-95	0.0012508622322766623
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	927.0
20-21	3921.0
22-23	6234.0
24-25	8892.0
26-27	14189.0
28-29	28150.0
30-31	57115.0
32-33	71352.0
34-35	93299.0
36-37	130199.0
38-39	171309.0
40-41	170600.0
42-43	196270.0
44-45	245755.0
46-47	237264.0
48-49	205622.0
50-51	177987.0
52-53	155719.0
54-55	162505.0
56-57	171373.0
58-59	176991.0
60-61	180809.0
62-63	162773.0
64-65	164921.0
66-67	177426.0
68-69	186514.0
70-71	194144.0
72-73	175840.0
74-75	172538.0
76-77	180558.0
78-79	197011.0
80-81	207003.0
82-83	194881.0
84-85	185460.0
86-87	191934.0
88-89	211782.0
90-91	229417.0
92-93	233145.0
94-95	268696.0
96-97	724232.0
98-99	1374238.0
100-101	1.5083524E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.88834631832833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.62780139457372	65.8378004810302
2	9.00243012621547	13.843639305020144
3	2.5861052157682933	5.965240603368831
4	1.1368242377093358	3.4963414276826006
5	0.567167954742284	2.180430306244135
6	0.3133164317557483	1.4454229387235313
7	0.2068061372869082	1.1130687333130087
8	0.13333394257688866	0.8201461082271935
9	0.09692140786980216	0.670691409655797
>10	0.31800707668622863	3.736541768485856
>50	0.008177953686762487	0.4264549225610754
>100	0.0030079439328049556	0.3978490414759285
>500	5.7283225861426655E-5	0.02897310900960363
>1k	4.2893969877202846E-5	0.037399845202104966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.3135950842960594E-5	0.0	1.7254380337184237E-5	0.0
3	0.0	4.7449545927256654E-5	0.0	2.5881570505776357E-5	0.0
4	0.0	8.195830660162513E-5	0.0	4.3135950842960594E-5	0.0
5	1.2940785252888179E-5	8.195830660162513E-5	0.0	4.7449545927256654E-5	0.0
6	1.2940785252888179E-5	8.627190168592119E-5	0.0	4.7449545927256654E-5	0.0
7	1.2940785252888179E-5	9.058549677021725E-5	0.0	5.1763141011552714E-5	0.0
8	1.7254380337184237E-5	1.0783987710740149E-4	0.0	5.6076736095848774E-5	0.0
9	1.7254380337184237E-5	1.811709935404345E-4	0.0	6.0390331180144834E-5	0.0
10-11	2.5881570505776357E-5	1.811709935404345E-4	0.0	1.1215347219169755E-4	0.0
12-13	3.8822355758664534E-5	1.811709935404345E-4	0.0	1.1862386481814164E-4	0.0
14-15	4.3135950842960594E-5	2.394045271784313E-4	0.0	1.2509425744458572E-4	0.0
16-17	5.6076736095848774E-5	2.4371812226272737E-4	0.0	1.2940785252888178E-4	0.0
18-19	6.470392626444089E-5	2.545021099734675E-4	0.0	1.380350426974739E-4	0.0
20-21	6.901752134873695E-5	2.631293001420596E-4	0.0	1.4666223286606602E-4	0.0
22-23	7.548791397518104E-5	3.041084534428722E-4	0.0	1.5097582795036208E-4	0.0
24-25	8.411510414377316E-5	3.386172141172407E-4	0.0	1.8332779108258253E-4	0.0
26-27	9.489909185451331E-5	3.5802839199657293E-4	0.0	2.394045271784313E-4	0.0
28-29	9.489909185451331E-5	3.5802839199657293E-4	0.0	4.572410789353823E-4	0.0
30-31	9.921268693880937E-5	3.7959636741805323E-4	0.0	0.001235844991650821	0.0
32-33	1.0352628202310543E-4	3.839099625023493E-4	0.0	0.0027434464736122936	0.0
34-35	1.0783987710740149E-4	4.33516305971754E-4	0.0	0.004565940396727379	0.0
36-37	1.1215347219169755E-4	4.8743624452545473E-4	0.0	0.007315857262966116	0.0
38-39	1.1215347219169755E-4	5.025338273204909E-4	0.0	0.012034930285186006	0.0
40-41	1.1215347219169755E-4	5.391993855370075E-4	0.0	0.01754770480291637	0.0
42-43	1.1215347219169755E-4	5.607673609584877E-4	0.0	0.02244794881867669	0.0
44-45	1.1215347219169755E-4	6.276280847650767E-4	0.0	0.02785072666175751	0.0
46-47	1.1215347219169755E-4	6.772344282344814E-4	0.0	0.03383799663876044	0.0
48-49	1.1215347219169755E-4	6.793912257766294E-4	0.0	0.039732524321451	0.0
50-51	1.1215347219169755E-4	7.635063299204025E-4	0.0	0.04601096196664392	0.0
52-53	1.1646706727599361E-4	7.635063299204025E-4	0.0	0.052200970912608766	2.1567975421480296E-6
54-55	1.1646706727599361E-4	7.721335200889946E-4	0.0	0.05761884633848462	4.313595084296059E-6
56-57	1.1646706727599361E-4	7.850743053418828E-4	0.0	0.06312946405867283	4.313595084296059E-6
58-59	1.1646706727599361E-4	7.850743053418828E-4	0.0	0.06900458056348406	4.313595084296059E-6
60-61	1.2509425744458572E-4	7.95858293052623E-4	0.0	0.0746640173140805	4.313595084296059E-6
62-63	1.2509425744458572E-4	8.260534586426954E-4	0.0	0.08012287189325715	4.313595084296059E-6
64-65	1.2509425744458572E-4	8.950709799914324E-4	0.0	0.08516977814188355	4.313595084296059E-6
66-67	1.2509425744458572E-4	9.015413726178764E-4	0.0	0.0901519804642455	4.313595084296059E-6
68-69	1.3372144761317784E-4	9.209525504972086E-4	0.0	0.09526790423422063	4.313595084296059E-6
70-71	1.3372144761317784E-4	9.317365382079488E-4	0.0	0.0998295310358637	4.313595084296059E-6
72-73	1.3372144761317784E-4	9.360501332922449E-4	0.0	0.10344001012141951	4.313595084296059E-6
74-75	1.3372144761317784E-4	9.511477160872811E-4	0.0	0.10609718469334588	4.313595084296059E-6
76-77	1.3372144761317784E-4	0.0010029108570988338	0.0	0.10876082965789868	4.313595084296059E-6
78-79	1.3372144761317784E-4	0.0010266356300624622	0.0	0.110557442010508	4.313595084296059E-6
80-81	1.3372144761317784E-4	0.0010266356300624622	0.0	0.1112756555920433	4.313595084296059E-6
82-83	1.3372144761317784E-4	0.0010352628202310544	0.0	0.11174583745623157	4.313595084296059E-6
84-85	1.3372144761317784E-4	0.0010395764153153504	0.0	0.1119183812596034	4.313595084296059E-6
86-87	1.380350426974739E-4	0.0010525172005682384	0.0	0.11199818276866289	4.313595084296059E-6
88	1.380350426974739E-4	0.0011172211268326794	0.0	0.11200680995883149	4.313595084296059E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	39185	0.0	50.181145	1
GTATCAA	70715	0.0	37.129475	1
TCAACGC	88990	0.0	28.914183	4
ATCAACG	89220	0.0	28.830145	3
CAACGCA	90190	0.0	28.515377	5
TATCAAC	91915	0.0	28.270624	2
AACGCAG	93045	0.0	27.746347	6
ACGCAGA	104885	0.0	24.541494	7
CGCAGAG	106730	0.0	24.125193	8
GTACATG	82870	0.0	21.04393	1
GCAGAGT	122195	0.0	20.99565	9
TACATGG	84170	0.0	20.42695	2
ACATGGG	83710	0.0	20.119102	3
GAGTACT	72845	0.0	19.29309	12-13
AGAGTAC	111810	0.0	19.060839	10-11
CAGAGTA	119500	0.0	18.672523	10-11
CATGGGG	58560	0.0	18.160236	4
AGTACTT	78210	0.0	17.509293	12-13
GTACTTT	81550	0.0	17.208584	14-15
ACTTTTT	91915	0.0	15.462608	16-17
>>END_MODULE