FastQCFastQC Report
Fri 27 May 2016
SRR2140256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8593894
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.033.65511
GTATCAA99300.030.7970091
TCAACGC99100.029.0680714
CAACGCA102300.028.1982945
ATCAACG102050.028.059073
AACGCAG108300.026.5134856
TATCAAC117400.025.0541212
ACGCAGA129900.022.104397
CGCAGAG136950.020.9330838
TGTAGAT118650.020.69271594
GTACATG235550.020.6405661
TACATGG236950.019.7153932
GGTGTAG129600.019.60286794
GTGTAGA121700.019.31105894
ACATGGG236400.018.8276523
AGGTGTA159850.016.8377894
CATGGGG169700.015.8534634
GCAGAGT181500.015.580469
AGAGTAC151350.015.34161310-11
TTAGGTG200400.015.33069194
Produced by FastQC (version 0.11.5)