##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140256_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8593894
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319397818963093	31.0	31.0	34.0	28.0	34.0
2	31.439721504593845	31.0	31.0	34.0	28.0	34.0
3	31.662227856196505	33.0	31.0	34.0	28.0	34.0
4	35.25213564421437	37.0	35.0	37.0	32.0	37.0
5	35.37521652000827	37.0	35.0	37.0	33.0	37.0
6	35.41042651910764	37.0	35.0	37.0	33.0	37.0
7	35.31692303861323	37.0	35.0	37.0	32.0	37.0
8	35.2875737122194	37.0	35.0	37.0	32.0	37.0
9	36.92501583100746	39.0	37.0	39.0	33.0	39.0
10-11	36.946548095659544	39.0	37.0	39.0	33.0	39.0
12-13	36.899480200709945	39.0	37.0	39.0	33.0	39.0
14-15	38.24040813163393	40.0	38.0	41.0	33.0	41.0
16-17	38.2326065459965	40.0	38.0	41.0	33.0	41.0
18-19	38.245251395932975	40.0	38.0	41.0	33.0	41.0
20-21	38.30692701941638	40.0	38.0	41.0	33.0	41.0
22-23	38.274927242803464	40.0	38.0	41.0	33.0	41.0
24-25	38.303648586709905	40.0	38.0	41.0	33.0	41.0
26-27	38.28998914845757	40.0	38.0	41.0	33.0	41.0
28-29	38.22260996473966	40.0	38.0	41.0	33.0	41.0
30-31	38.154047493295245	40.0	38.0	41.0	32.5	41.0
32-33	38.114671691982494	40.0	38.0	41.0	32.0	41.0
34-35	38.04037834806269	40.0	38.0	41.0	32.0	41.0
36-37	37.95745090646721	40.0	38.0	41.0	32.0	41.0
38-39	37.87378393244073	40.0	37.0	41.0	32.0	41.0
40-41	37.83301110658366	40.0	37.0	41.0	32.0	41.0
42-43	37.674686850874394	40.0	37.0	41.0	31.0	41.0
44-45	37.49255439845642	40.0	37.0	41.0	31.0	41.0
46-47	37.34912744879739	40.0	36.0	41.0	31.0	41.0
48-49	37.21523399633992	39.0	36.0	41.0	31.0	41.0
50-51	37.09151374561285	39.0	36.0	40.5	30.0	41.0
52-53	36.951886863289786	39.0	35.5	40.0	30.0	41.0
54-55	36.78190747498248	39.0	35.0	40.0	30.0	41.0
56-57	36.76409113528014	39.0	35.0	40.0	30.0	41.0
58-59	36.8399130003269	39.0	35.0	40.0	30.0	41.0
60-61	37.14197820188366	39.0	35.0	41.0	31.0	41.0
62-63	37.11168623652262	39.0	35.0	41.0	31.0	41.0
64-65	36.9080439227409	39.0	35.0	41.0	31.0	41.0
66-67	36.696006826674704	38.0	35.0	40.0	31.0	41.0
68-69	36.417548354317724	37.5	35.0	40.0	31.0	41.0
70-71	36.16449934795509	37.0	35.0	40.0	30.5	41.0
72-73	35.861535613657594	36.5	34.5	39.0	30.0	41.0
74-75	35.52752377225397	36.0	34.0	39.0	30.0	41.0
76-77	35.20144737882995	36.0	34.0	38.5	30.0	40.0
78-79	34.87057830698425	35.0	34.0	37.0	30.0	39.0
80-81	34.546693110922945	35.0	34.0	37.0	30.0	39.0
82-83	34.27224479940477	35.0	34.0	36.5	29.0	39.0
84-85	34.02424274124859	35.0	34.0	36.0	29.0	37.0
86-87	33.83228057157798	35.0	33.5	36.0	29.0	37.0
88-89	33.70788653138993	35.0	33.0	35.0	29.0	37.0
90-91	33.58396573155492	35.0	33.0	35.0	29.0	36.0
92-93	33.506880149047916	35.0	33.0	35.0	29.0	36.0
94-95	33.47391031416231	35.0	33.0	35.0	29.0	36.0
96-97	33.472537610691276	35.0	33.0	35.0	29.5	36.0
98-99	33.51086315167294	35.0	33.0	35.0	30.0	35.0
100	32.774444981831024	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	55.0
17	286.0
18	1299.0
19	4644.0
20	11566.0
21	23216.0
22	37150.0
23	51110.0
24	66476.0
25	82084.0
26	93201.0
27	99025.0
28	112836.0
29	134935.0
30	158799.0
31	189129.0
32	235633.0
33	302758.0
34	408656.0
35	573729.0
36	898555.0
37	1609815.0
38	2268199.0
39	1181962.0
40	48772.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.466650624268816	20.34517763425986	14.188120076882493	24.000051664588835
2	13.965876722537962	20.957508363936316	42.15550753627088	22.92110737725484
3	17.92874243621631	26.26718327546896	30.225094943743724	25.578979344571014
4	12.322071180838435	18.0966341565606	39.65818998626661	29.92310467633435
5	13.313296736636099	38.10938813396063	35.7111294698452	12.866185659558083
6	29.579746771803677	38.13464740634053	19.38722807808094	12.89837774377485
7	26.34779578769883	32.75164712492732	23.50187627697861	17.39868081039524
8	23.838450201041827	37.28338577852189	21.6418032021936	17.236360818242687
9	24.96454455284712	17.914321394144952	21.713465064224742	35.407668988783186
10-11	23.62398443824443	27.31296040709858	29.353549946944856	19.70950520771213
12-13	24.289313374472655	24.80556122673695	29.24048455116855	21.66464084762185
14-15	21.66737560168569	26.356912389885984	26.514739884847078	25.46097212358125
16-17	21.690681011487683	29.389494693098495	27.194414987031724	21.725409308382098
18-19	21.759659925969526	29.013554799114043	28.198336411778897	21.028448863137527
20-21	22.581192923254928	28.144188698299484	27.962169481848314	21.312448896597274
22-23	22.283695447076298	28.03832098344266	27.76683835167248	21.911145217808567
24-25	22.048258436183474	28.170834136462876	27.82374224666612	21.95716518068753
26-27	22.061167511223914	28.2832083981558	27.71612838312892	21.939495707491368
28-29	21.991334093660488	28.33044534068019	27.76449500488202	21.913725560777312
30-31	22.24581761762335	28.12963542831057	27.7237446735473	21.90080228051877
32-33	22.021130193793983	28.24370726435792	27.73063896877932	22.00452357306878
34-35	22.15514176674585	28.229569602610493	27.75457507645728	21.860713554186376
36-37	22.1382628324411	28.116289171454607	27.78095781894518	21.964490177159117
38-39	22.00783001583694	28.184397627411222	27.8506450290841	21.95712732766774
40-41	22.194164524698063	28.026520976952778	27.8573741675599	21.921940330789262
42-43	22.02628072079155	28.09308590264018	27.79551333835365	22.08512003821462
44-45	22.07234494382498	28.09403174760491	27.819347945506813	22.014275363063298
46-47	22.037384141916448	28.01471336212456	27.78827204449028	22.159630451468708
48-49	21.97147564273507	27.997248106679134	27.916984551487168	22.11429169909863
50-51	21.792156559267838	28.000583139881975	28.020585478105442	22.186674822744745
52-53	21.96053675526985	27.856232566774786	27.967763209661427	22.215467468293934
54-55	21.968995072980338	27.674203570333695	28.080176131595074	22.276625225090896
56-57	21.81758083633999	27.565152888856492	28.197213819783112	22.420052455020404
58-59	21.73168599489134	27.523124951105117	28.330210269725903	22.414978784277643
60-61	21.62539040246698	27.382339057898896	28.664119931936412	22.328150607697715
62-63	21.488876495282227	27.598563243232782	28.680880892065037	22.23167936941995
64-65	21.37521867800265	27.87178219919657	28.374186704843396	22.378812417957377
66-67	21.22573592709517	28.061263085171966	28.126090603162908	22.586910384569958
68-69	21.340253779662955	28.24190613414288	28.14444970958268	22.273390376611484
70-71	21.351887819349884	28.271934006619816	27.807433934383276	22.56874423964703
72-73	21.692650316557852	28.124282885412487	27.444242773861	22.738824024168657
74-75	21.89773225988178	27.975784833652	27.454650362365385	22.67183254410083
76-77	22.12305138835673	27.55375640760212	27.575577351666762	22.747614852374387
78-79	22.371497607651943	27.362986301049148	27.403599142190753	22.861916949108156
80-81	22.74572318966391	27.3837449714788	27.123877568345446	22.746654270511847
82-83	22.45470884111517	27.65132807286429	27.02483195005542	22.86913113596512
84-85	22.807058240774474	27.65714589270764	27.009858757533323	22.525937108984564
86-87	22.719724473386453	27.889742540705377	27.006066794605683	22.384466191302494
88-89	22.572393981150768	28.141494352714286	26.938046268542443	22.3480653975925
90-91	22.779229259542365	28.213842024578963	27.263198731194105	21.743729984684563
92-93	22.887921000057553	28.01254290539974	27.518863451946874	21.58067264259583
94-95	22.57526059215849	28.041206692333247	27.93118452346315	21.45234819204511
96-97	22.837537714708684	27.760798084170368	28.161903025486488	21.23976117563446
98-99	23.56083512176876	29.83740648545617	28.623288080107436	17.978470312667632
100	22.76592304549729	31.059083156586436	22.395772814896144	23.77922098302013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3000.0
1	2209.0
2	1541.0
3	1618.0
4	1539.5
5	1597.0
6	1860.5
7	2255.5
8	2601.5
9	2956.5
10	3368.0
11	3604.5
12	3831.5
13	4286.0
14	4964.5
15	5872.5
16	7032.0
17	8394.5
18	10098.5
19	12484.5
20	15679.5
21	19840.5
22	25559.0
23	33327.5
24	43032.5
25	55551.0
26	71398.0
27	89494.0
28	108794.5
29	129209.5
30	149928.5
31	170214.5
32	190927.0
33	210194.5
34	228788.0
35	247093.0
36	266852.5
37	286563.0
38	302187.0
39	315418.0
40	328865.5
41	340405.5
42	347964.0
43	355396.0
44	361176.0
45	366839.5
46	369245.0
47	367984.0
48	365378.0
49	359214.0
50	352841.0
51	341354.5
52	323693.0
53	303084.5
54	281248.5
55	258590.5
56	233827.5
57	207941.0
58	182698.0
59	158415.0
60	132401.0
61	107473.5
62	85951.5
63	67229.0
64	51727.0
65	39870.0
66	31028.0
67	24220.5
68	18977.5
69	14890.5
70	11669.5
71	9080.5
72	6986.0
73	5278.0
74	3974.0
75	2972.5
76	2236.5
77	1719.0
78	1367.0
79	1051.0
80	782.5
81	584.0
82	448.0
83	320.5
84	226.0
85	176.0
86	146.5
87	121.0
88	82.5
89	48.5
90	33.5
91	27.5
92	20.0
93	8.0
94	2.5
95	3.0
96	2.5
97	3.5
98	7.5
99	9.0
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01221797708931481
3	0.022853435241346937
4	0.019711669704094558
5	0.01106599639232227
6	0.013614317328093645
7	0.015662283011635938
8	0.0018617869850384473
9	1.1636168656490296E-4
10-11	5.818084328245148E-5
12-13	0.002914860248450819
14-15	2.0363295148858018E-4
16-17	4.3635632461838604E-4
18-19	5.818084328245147E-6
20-21	1.1642510204369131E-4
22-23	1.1651113692091707E-5
24-25	2.3321360535523737E-5
26-27	0.0025503272355806715
28-29	0.0035878269356165626
30-31	0.002651777375737888
32-33	0.003966653820028456
34-35	0.005967902571812878
36-37	0.0011817913605260768
38-39	0.0013960123471648653
40-41	6.034519482864384E-4
42-43	6.0070732085616174E-5
44-45	8.460541605196803E-5
46-47	0.004124798642296367
48-49	7.660431810574313E-4
50-51	2.6472707500469123E-4
52-53	0.004341712137815207
54-55	2.1035765193591835E-4
56-57	1.5495007539560767E-4
58-59	5.401691176374717E-4
60-61	6.219938647769167E-5
62-63	0.0
64-65	6.296742292220727E-6
66-67	0.0017163296176704141
68-69	0.0
70-71	3.4382786809206026E-4
72-73	0.0015599712939063917
74-75	1.5229308021958544E-4
76-77	2.007446153100825E-5
78-79	0.0
80-81	0.003217602338580764
82-83	1.1775708223995307E-4
84-85	3.6442663426072906E-4
86-87	0.013538725755556993
88-89	6.535751963760045E-4
90-91	8.154961748132551E-4
92-93	7.388082918818784E-5
94-95	1.8771234959547988E-4
96-97	5.3760183042671224E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3206.0
20-21	6108.0
22-23	6845.0
24-25	7813.0
26-27	10223.0
28-29	13334.0
30-31	20097.0
32-33	23598.0
34-35	31118.0
36-37	42671.0
38-39	48178.0
40-41	46293.0
42-43	47344.0
44-45	53609.0
46-47	59656.0
48-49	37373.0
50-51	36912.0
52-53	14415.0
54-55	14472.0
56-57	14058.0
58-59	14169.0
60-61	15118.0
62-63	68683.0
64-65	73543.0
66-67	78265.0
68-69	79336.0
70-71	79697.0
72-73	77314.0
74-75	78153.0
76-77	81652.0
78-79	85711.0
80-81	85771.0
82-83	83645.0
84-85	83843.0
86-87	87127.0
88-89	93106.0
90-91	98598.0
92-93	104444.0
94-95	127632.0
96-97	308420.0
98-99	580863.0
100-101	5671481.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.51862068985105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.72530756735526	76.73184544132877
2	7.226184316596732	12.35946631211973
3	1.751634904289991	4.493922029012379
4	0.6221428575227927	2.1281919618956704
5	0.2865940084673326	1.2254562151050885
6	0.1324192382301219	0.679458636374449
7	0.08123951213977648	0.4863243716597066
8	0.05239238878582375	0.3584419858888054
9	0.02799411990565181	0.21546166695818816
>10	0.09243558976259142	1.181491661252751
>50	0.0012308810451514979	0.0665169972101703
>100	4.039383604932779E-4	0.058533512673880614
>500	2.067753825826779E-5	0.014889208520335142
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3272337312980587E-5	0.0	0.0	0.0
3	0.0	2.3272337312980587E-5	0.0	1.1636168656490293E-5	0.0
4	0.0	6.981701193894176E-5	0.0	3.490850596947088E-5	0.0
5	0.0	6.981701193894176E-5	0.0	5.818084328245147E-5	0.0
6	0.0	6.981701193894176E-5	0.0	5.818084328245147E-5	0.0
7	0.0	8.145318059543206E-5	0.0	6.981701193894176E-5	0.0
8	0.0	1.2799785522139324E-4	0.0	6.981701193894176E-5	0.0
9	0.0	2.559957104427865E-4	0.0	6.981701193894176E-5	0.0
10-11	0.0	2.559957104427865E-4	0.0	1.0472551790841265E-4	0.0
12-13	0.0	2.618137947710316E-4	0.0	1.1636168656490294E-4	0.0
14-15	0.0	3.1417655372523797E-4	0.0	1.3381593954963839E-4	0.0
16-17	0.0	3.2581272238172825E-4	0.0	1.8036061417559955E-4	0.0
18-19	0.0	3.4326697536646365E-4	0.0	2.094510358168253E-4	0.0
20-21	0.0	3.607212283511991E-4	0.0	2.3854145745805103E-4	0.0
22-23	0.0	4.479924932748763E-4	0.0	3.025403850687476E-4	0.0
24-25	0.0	5.11991420885573E-4	0.0	4.596286619313666E-4	0.0
26-27	0.0	5.236275895420632E-4	0.0	9.250754081909784E-4	0.0
28-29	0.0	5.236275895420632E-4	0.0	0.003758482476046365	0.0
30-31	0.0	5.468999268550438E-4	0.0	0.012683423835574421	0.0
32-33	0.0	5.585360955115341E-4	0.0	0.040121509527578536	0.0
34-35	0.0	6.516254447634565E-4	0.0	0.07735724922834747	0.0
36-37	0.0	7.330786253588886E-4	0.0	0.130674174012386	0.0
38-39	0.0	7.621690470001143E-4	0.0	0.19501055051412083	0.0
40-41	0.0	8.261679746108108E-4	0.0	0.25519863288981687	0.0
42-43	0.0	8.436222275955463E-4	0.0	0.35941797746167226	0.0
44-45	0.0	9.134392395344881E-4	0.0	0.4623224349753441	0.0
46-47	0.0	9.774381671451848E-4	0.0	0.5831116837140417	5.818084328245147E-6
48-49	0.0	9.774381671451848E-4	0.0	0.6660484758131762	1.1636168656490293E-5
50-51	0.0	0.0010937998537100875	0.0	1.006639132388647	1.1636168656490293E-5
52-53	0.0	0.0010937998537100875	0.0	1.4601820781126693	1.745425298473544E-5
54-55	0.0	0.0011054360223665778	0.0	2.0557619165421404	2.3272337312980587E-5
56-57	0.0	0.0011287083596795585	0.0	2.7065728294996427	2.3272337312980587E-5
58-59	0.0	0.001146162612664294	0.0	3.3788350193753844	2.3272337312980587E-5
60-61	0.0	0.0011927072872902553	0.0	4.047978716051187	2.3272337312980587E-5
62-63	0.0	0.0012334338775879712	0.0	4.534620743518596	2.3272337312980587E-5
64-65	0.0	0.0013497955641528741	0.0	4.542591519048292	2.3272337312980587E-5
66-67	0.0	0.0013614317328093644	0.0	4.545774011175842	2.3272337312980587E-5
68-69	0.0	0.0013788859857940998	0.0	4.548613236328025	2.3272337312980587E-5
70-71	0.0	0.001384704070122345	0.0	4.550992832818277	2.3272337312980587E-5
72-73	0.0	0.001384704070122345	0.0	4.553343338886888	2.3272337312980587E-5
74-75	0.0	0.0014021583231070803	0.0	4.555391304570431	2.3272337312980587E-5
76-77	0.0	0.001466157250717777	0.0	4.557206546880844	2.3272337312980587E-5
78-79	0.0	0.001501065756687248	0.0	4.558567978613652	2.3272337312980587E-5
80-81	0.0	0.001506883841015493	0.0	4.55930687532334	2.9090421641225733E-5
82-83	0.0	0.0015127019253437382	0.0	4.559749049732286	4.6544674625961174E-5
84-85	0.0	0.0015243380940002285	0.0	4.559847957165867	4.6544674625961174E-5
86-87	0.0	0.0015534285156414544	0.0	4.559900319924822	4.6544674625961174E-5
88	0.0	0.0016406997805651314	0.0	4.559911956093478	4.6544674625961174E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5925	0.0	33.6551	1
GTATCAA	9930	0.0	30.797009	1
TCAACGC	9910	0.0	29.068071	4
CAACGCA	10230	0.0	28.198294	5
ATCAACG	10205	0.0	28.05907	3
AACGCAG	10830	0.0	26.513485	6
TATCAAC	11740	0.0	25.054121	2
ACGCAGA	12990	0.0	22.10439	7
CGCAGAG	13695	0.0	20.933083	8
TGTAGAT	11865	0.0	20.692715	94
GTACATG	23555	0.0	20.640566	1
TACATGG	23695	0.0	19.715393	2
GGTGTAG	12960	0.0	19.602867	94
GTGTAGA	12170	0.0	19.311058	94
ACATGGG	23640	0.0	18.827652	3
AGGTGTA	15985	0.0	16.83778	94
CATGGGG	16970	0.0	15.853463	4
GCAGAGT	18150	0.0	15.58046	9
AGAGTAC	15135	0.0	15.341613	10-11
TTAGGTG	20040	0.0	15.330691	94
>>END_MODULE