FastQCFastQC Report
Fri 27 May 2016
SRR2140255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7518582
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.038.105761
GTATCAA99550.031.8878691
TCAACGC107500.027.9147054
ATCAACG109200.027.5601083
CAACGCA111950.026.7247835
TATCAAC121150.025.8445782
AACGCAG116850.025.8246
ACGCAGA141900.021.1117487
CGCAGAG149250.019.9247138
GTACATG231250.019.7658061
TACATGG232950.019.230622
ACATGGG239100.017.9737283
ACCGAAC11350.017.568898
AGGACCG15950.016.3109685
GCAGAGT180700.016.2646689
CATGGGG179150.016.0000044
GGACCGA14200.015.5712986
AGAGTAC157400.015.34005810-11
GTATATA69250.014.5286031
GTGGTAT46000.014.51839351
Produced by FastQC (version 0.11.5)