FastQCFastQC Report
Fri 27 May 2016
SRR2140254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8532876
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67750.040.4155771
GTATCAA117500.032.624831
TCAACGC126850.028.7009854
CAACGCA128900.028.2091925
ATCAACG128550.028.1894683
AACGCAG135950.026.5548956
TATCAAC141850.026.3005772
ACGCAGA161650.022.2256457
CGCAGAG169100.021.2949228
GTACATG284900.020.768631
TACATGG281150.020.2695562
ACATGGG284500.019.489393
CATGGGG212600.017.3863414
GCAGAGT213900.016.4948439
AGAGTAC181200.015.64814710-11
CAGAGTA206750.014.87273810-11
GTGGTAT53950.014.3537451
GTACTAG21900.014.2612271
TGGTATC52050.013.4803522
GTGTAGC43050.013.3171031
Produced by FastQC (version 0.11.5)