FastQCFastQC Report
Fri 27 May 2016
SRR2140253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832045
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.044.416811
GTATCAA95100.034.565931
TCAACGC108250.029.024454
ATCAACG110350.028.514433
CAACGCA113700.027.6694325
AACGCAG117200.026.8774686
TATCAAC121450.026.3067532
ACGCAGA141550.022.2534087
GTACATG245200.021.632861
TACATGG239650.021.3454782
CGCAGAG152800.020.5273828
ACATGGG244100.020.5239543
CATGGGG187250.018.7738784
GCAGAGT183050.016.8948849
GTGGTAT38850.016.5153961
TGGTATC36450.016.156142
GTATAGG19350.016.125181
AGAGTAC154600.015.96905310-11
CAGAGTA174100.015.92145910-11
GTATAAG27300.014.6489311
Produced by FastQC (version 0.11.5)