##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6832045
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17442830075036	31.0	31.0	34.0	28.0	34.0
2	31.280746394381186	31.0	31.0	34.0	28.0	34.0
3	31.516539337782465	31.0	31.0	34.0	28.0	34.0
4	35.09418746510013	37.0	35.0	37.0	32.0	37.0
5	35.227864277826036	37.0	35.0	37.0	32.0	37.0
6	35.261367277293985	37.0	35.0	37.0	32.0	37.0
7	35.15296796200845	37.0	35.0	37.0	32.0	37.0
8	35.12182530999137	37.0	35.0	37.0	32.0	37.0
9	36.73226962058944	39.0	37.0	39.0	32.0	39.0
10-11	36.75238109526504	39.0	37.0	39.0	32.0	39.0
12-13	36.69637707304328	39.0	37.0	39.0	32.0	39.0
14-15	38.01749498137088	40.0	38.0	41.0	32.0	41.0
16-17	38.01179624841464	40.0	38.0	41.0	32.0	41.0
18-19	38.028374813105	40.0	38.0	41.0	32.0	41.0
20-21	38.093185522321434	40.0	38.0	41.0	32.0	41.0
22-23	38.06950482716794	40.0	38.0	41.0	32.0	41.0
24-25	38.099462335119185	40.0	38.0	41.0	32.0	41.0
26-27	38.084266080654615	40.0	38.0	41.0	32.0	41.0
28-29	38.018506754333366	40.0	38.0	41.0	32.0	41.0
30-31	37.94846216874524	40.0	38.0	41.0	32.0	41.0
32-33	37.90686677764966	40.0	37.0	41.0	32.0	41.0
34-35	37.825339637211314	40.0	37.0	41.0	31.5	41.0
36-37	37.73910710694473	40.0	37.0	41.0	31.0	41.0
38-39	37.64917772902056	40.0	37.0	41.0	31.0	41.0
40-41	37.60231106159978	40.0	37.0	41.0	31.0	41.0
42-43	37.43548680460594	40.0	36.5	41.0	31.0	41.0
44-45	37.24009748234252	39.5	36.0	41.0	30.0	41.0
46-47	37.08588541128232	39.0	36.0	41.0	30.0	41.0
48-49	36.936797194034035	39.0	35.0	40.0	30.0	41.0
50-51	36.79703374556364	39.0	35.0	40.0	30.0	41.0
52-53	36.6358236201317	39.0	35.0	40.0	30.0	41.0
54-55	36.44988336141881	38.0	35.0	40.0	29.5	41.0
56-57	36.41643021256054	38.0	34.5	40.0	29.5	41.0
58-59	36.47353137736833	38.0	35.0	40.0	30.0	41.0
60-61	36.7717982367795	39.0	35.0	40.0	30.5	41.0
62-63	36.72164354225942	38.5	35.0	40.0	31.0	41.0
64-65	36.485704580582	38.0	35.0	40.0	30.0	41.0
66-67	36.245077678109865	37.0	35.0	40.0	30.0	41.0
68-69	35.93934569028335	37.0	34.0	40.0	30.0	41.0
70-71	35.67267161602308	36.0	34.0	39.0	30.0	41.0
72-73	35.35462153904825	36.0	34.0	39.0	30.0	41.0
74-75	35.0339133612313	35.5	34.0	38.5	29.0	40.0
76-77	34.72269366249482	35.0	34.0	37.0	29.0	39.5
78-79	34.41412791944781	35.0	34.0	37.0	29.0	39.0
80-81	34.11801356795398	35.0	33.0	36.5	29.0	39.0
82-83	33.877804070118096	35.0	33.0	36.0	29.0	37.5
84-85	33.6637331188871	35.0	33.0	36.0	29.0	37.0
86-87	33.48964573313326	35.0	33.0	35.0	29.0	37.0
88-89	33.35372923165889	35.0	33.0	35.0	29.0	36.0
90-91	33.229639283722605	35.0	33.0	35.0	29.0	36.0
92-93	33.158276843868414	35.0	33.0	35.0	29.0	36.0
94-95	33.153430900973305	35.0	33.0	35.0	29.0	36.0
96-97	33.194368164759965	35.0	33.0	35.0	29.0	35.0
98-99	33.31697396176244	35.0	33.0	35.0	29.0	35.0
100	32.58313162331875	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	52.0
17	314.0
18	1231.0
19	4187.0
20	10602.0
21	21215.0
22	34305.0
23	47961.0
24	63256.0
25	77863.0
26	86792.0
27	90166.0
28	101819.0
29	119756.0
30	140715.0
31	165968.0
32	206422.0
33	265493.0
34	359868.0
35	511305.0
36	816430.0
37	1383950.0
38	1619153.0
39	674436.0
40	28785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07375683269065	19.59886095598024	14.154971168954535	24.172411042374577
2	14.545431922016745	20.419749941811187	41.39287183383325	23.641946302338823
3	18.418667815241474	25.341227149895744	29.540367065691004	26.699737969171778
4	12.748246280395744	17.47130300301562	38.0366070164595	31.743843700129137
5	14.005223607708874	37.53123493147857	34.765609560941165	13.697931899871385
6	31.063097098967468	36.71343084931185	18.66166390041858	13.561808151302108
7	27.70851101392782	32.13205880535986	22.195442651950394	17.963987528761933
8	24.973115230165394	36.423970661313376	20.613749984448038	17.989164124073195
9	25.562369045240548	17.61795716795947	20.82890383319172	35.99076995360826
10-11	24.536794884283157	26.794472254165242	28.157395367872002	20.511337493679598
12-13	25.140752628426526	24.284131780526604	28.12762557224667	22.447490018800195
14-15	22.466751663322494	25.655450899062483	25.61227927234256	26.26551816527246
16-17	22.47981801579518	28.764224468555906	26.163376388672077	22.59258112697684
18-19	22.605936085891717	28.292806662252172	27.270689415934672	21.830567835921432
20-21	23.62458354806031	27.33343258925325	26.988253406075767	22.053730456610676
22-23	23.328209650262817	27.209601151692535	26.78484855814867	22.677340639895974
24-25	22.97313568103705	27.35606021196234	26.94127918319592	22.729524923804686
26-27	22.924095266589973	27.448296724159597	26.86142554632049	22.766182462929937
28-29	22.88302949465342	27.46296020182469	26.888217078201183	22.76579322532071
30-31	23.183500360797	27.239691132718413	26.81790304561292	22.758905460871667
32-33	22.910527975240324	27.330614544670784	26.83878873043431	22.92006874965458
34-35	23.04923502326223	27.309120269728616	26.903238082903048	22.73840662410611
36-37	23.08097171749372	27.086331830455467	26.924509273540558	22.908187178510246
38-39	22.81502027709644	27.20706854036456	27.125320243311794	22.85259093922721
40-41	22.985063164765965	27.00460806174166	27.153058135516485	22.857270637975894
42-43	22.71559953471554	27.019779063705627	27.178388597234765	23.086232804344068
44-45	22.657144274639876	27.005655813108316	27.23214685986411	23.1050530523877
46-47	22.61741344437977	26.914777628848675	27.2875587727969	23.180250153974658
48-49	22.493626989378303	26.947408003902623	27.388276211385953	23.17068879533312
50-51	22.254226725858082	26.9919082059412	27.505709424518127	23.24815564368259
52-53	22.423791995202485	26.922064498242364	27.44234770829786	23.211795798257285
54-55	22.409772265705914	26.756062034360333	27.536443465600108	23.297722234333644
56-57	22.34754148655631	26.92984998071536	27.421772784371267	23.300835748357063
58-59	22.27218049124458	26.90661793550194	27.477565010085968	23.34363656316751
60-61	22.278175030566942	26.73287296171958	27.494963981465965	23.493988026247514
62-63	22.27217838024534	26.763019057826504	27.463978213016144	23.500824348912015
64-65	22.401843257095177	26.780919815573146	27.361454768701314	23.45578215863036
66-67	22.310260660808154	26.9510899405785	27.041031369625017	23.697618028988327
68-69	22.28855287165765	27.053461381005008	26.95881210948588	23.699173637851466
70-71	22.37455134746949	26.961248548120555	26.854410090470495	23.809790013939462
72-73	22.50274990262836	27.01212028046219	26.455696101709357	24.029433715200092
74-75	22.489438951357094	26.95161427510493	26.407944679956998	24.151002093580985
76-77	22.791506268328284	26.750521526800725	26.411417243081036	24.046554961789955
78-79	22.79810940133842	26.562733358834194	26.597312661277456	24.04184457854993
80-81	23.011360057623275	26.467847235251575	26.51769363742169	24.003099069703456
82-83	22.99809651994392	26.51878622089823	26.544257285489792	23.938859973668063
84-85	23.058158019745246	26.388705402101543	26.631759549654983	23.92137702849823
86-87	22.726520630381515	26.666927066704922	26.796025170302496	23.810527132611067
88-89	22.853390004173608	26.776768784756698	26.595650202543347	23.77419100852634
90-91	22.83422633468494	26.96487645903018	26.782802548171585	23.418094658113297
92-93	22.62064825798394	26.961634713306587	27.03770236303517	23.3800146656743
94-95	22.389534092614753	27.044586624620486	27.297162958401312	23.26871632436345
96-97	22.444020747286526	27.007482470463934	27.539832869080783	23.008663913168764
98-99	23.85088139579873	29.06753506395247	27.532352223921986	19.54923131632681
100	23.153655545944847	30.2287672917731	21.161636167549016	25.45594099473304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3610.0
1	2600.5
2	1669.0
3	1684.5
4	1590.5
5	1591.0
6	1815.5
7	2145.0
8	2385.0
9	2711.5
10	3034.5
11	3218.0
12	3410.0
13	3722.5
14	4252.5
15	4853.0
16	5547.0
17	6512.5
18	7680.0
19	9060.5
20	10838.0
21	13180.5
22	16346.0
23	20570.0
24	26152.0
25	33606.0
26	42762.0
27	52888.0
28	63938.0
29	76619.0
30	88614.5
31	100274.5
32	114021.0
33	127452.0
34	140670.0
35	153447.0
36	167668.5
37	182710.0
38	194049.0
39	203944.5
40	215955.5
41	228158.0
42	238092.5
43	246779.5
44	255749.5
45	266749.0
46	276459.5
47	285032.5
48	291145.0
49	293762.0
50	294691.0
51	292522.5
52	283966.0
53	269844.5
54	255982.0
55	241210.5
56	224421.5
57	204567.5
58	183513.0
59	161871.5
60	136446.5
61	113841.0
62	93788.5
63	75349.0
64	60727.0
65	47884.5
66	38095.5
67	29238.5
68	21548.5
69	16936.5
70	13181.5
71	9547.0
72	7172.0
73	5442.5
74	4095.0
75	3084.0
76	2328.0
77	1766.0
78	1397.5
79	1148.5
80	917.5
81	719.5
82	565.0
83	380.5
84	241.0
85	171.5
86	127.5
87	92.0
88	54.5
89	35.0
90	27.5
91	26.5
92	22.5
93	12.0
94	6.5
95	5.5
96	6.5
97	7.0
98	5.5
99	5.5
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012221816454663281
3	0.022701841103212876
4	0.018705965783305
5	0.010992316356230089
6	0.013041483186952075
7	0.014153888037915442
8	0.0016100596527101327
9	1.4636905933728482E-4
10-11	2.1955358900592723E-5
12-13	0.0029420180926794247
14-15	1.6832441823787754E-4
16-17	3.439672894426193E-4
18-19	1.4636905933728483E-5
20-21	2.9293425727727423E-5
22-23	1.4660035248588752E-5
24-25	5.8697816815413054E-5
26-27	0.0023138755255435696
28-29	0.0036703947285187753
30-31	0.0025341709750642733
32-33	0.0039474963797400025
34-35	0.005551519020531676
36-37	9.999005284140993E-4
38-39	0.0012322965933012208
40-41	5.729906353238309E-4
42-43	3.7284784765377286E-5
44-45	8.966087566995353E-5
46-47	0.004050421382331703
48-49	6.30225762623816E-4
50-51	2.9320459997419796E-4
52-53	0.004045479018993185
54-55	2.038136554394279E-4
56-57	1.6640962682716825E-4
58-59	5.002033402366447E-4
60-61	2.2781395800933126E-5
62-63	0.0
64-65	1.5325225420656364E-5
66-67	0.001384052009427018
68-69	0.0
70-71	2.917351587228497E-4
72-73	0.0013301307183972292
74-75	8.046966603398733E-5
76-77	8.135508280116939E-6
78-79	1.646633178538837E-5
80-81	0.002486001434372774
82-83	1.014782420078485E-4
84-85	2.914823790757488E-4
86-87	0.011812298916921787
88-89	7.069371655690142E-4
90-91	6.473697143723899E-4
92-93	1.8322928047877077E-5
94-95	8.4167101997622E-5
96-97	6.72365314901815E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3121.0
20-21	5963.0
22-23	6575.0
24-25	7467.0
26-27	8942.0
28-29	10023.0
30-31	10739.0
32-33	11934.0
34-35	13298.0
36-37	14766.0
38-39	15896.0
40-41	15105.0
42-43	12895.0
44-45	13590.0
46-47	14071.0
48-49	13549.0
50-51	13746.0
52-53	13252.0
54-55	13725.0
56-57	12770.0
58-59	12869.0
60-61	14052.0
62-63	34520.0
64-65	57337.0
66-67	61505.0
68-69	62711.0
70-71	64283.0
72-73	63575.0
74-75	66379.0
76-77	70944.0
78-79	78624.0
80-81	81278.0
82-83	79893.0
84-85	82602.0
86-87	88582.0
88-89	95207.0
90-91	102191.0
92-93	108142.0
94-95	125177.0
96-97	270476.0
98-99	484130.0
100-101	4496141.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.52053428049268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.34165281407701	75.51204229348072
2	7.489906286180596	12.661017620376095
3	1.7528417834367747	4.444533721357436
4	0.6607380099491337	2.233837184813211
5	0.28420737623691755	1.2010679643000637
6	0.148665735092002	0.7539184415506921
7	0.08884759831181768	0.5256612535197401
8	0.056852186536146584	0.38441417448394744
9	0.04096594608563378	0.31162172854172626
>10	0.13252707196200217	1.7388648403991434
>50	0.002149883214798109	0.12396789652616501
>100	5.886720074655082E-4	0.08023525574826766
>500	5.6636909694976565E-5	0.028817624902789336
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.8547623734913925E-5	0.0	0.0	0.0
3	0.0	5.8547623734913925E-5	0.0	0.0	0.0
4	0.0	1.1709524746982785E-4	0.0	1.4636905933728481E-5	0.0
5	0.0	1.1709524746982785E-4	0.0	1.4636905933728481E-5	0.0
6	0.0	1.1709524746982785E-4	0.0	5.8547623734913925E-5	0.0
7	0.0	1.1709524746982785E-4	0.0	7.318452966864241E-5	0.0
8	0.0	1.1709524746982785E-4	0.0	7.318452966864241E-5	0.0
9	0.0	3.366488364757551E-4	0.0	8.782143560237089E-5	0.0
10-11	0.0	3.366488364757551E-4	0.0	1.244137004366921E-4	0.0
12-13	0.0	3.5128574240948356E-4	0.0	1.610059652710133E-4	0.0
14-15	0.0	4.3910717801185444E-4	0.0	1.6832441823787754E-4	7.318452966864241E-6
16-17	0.0	4.3910717801185444E-4	0.0	1.975982301053345E-4	2.9273811867456962E-5
18-19	0.0	4.537440839455829E-4	0.0	2.1955358900592722E-4	2.9273811867456962E-5
20-21	0.0	4.683809898793114E-4	0.0	3.000565716414339E-4	2.9273811867456962E-5
22-23	0.0	5.049732547136326E-4	0.0	7.245268437195598E-4	3.6592264834321205E-5
24-25	0.0	5.78157784382275E-4	0.0	0.0014490536874391196	4.3910717801185445E-5
26-27	0.0	6.001131432828678E-4	0.0	0.0037909586368356767	4.3910717801185445E-5
28-29	0.0	6.001131432828678E-4	0.0	0.01853032291210026	4.3910717801185445E-5
30-31	0.0	6.659792199846459E-4	0.0	0.06422674323720057	4.3910717801185445E-5
32-33	0.0	6.732976729515102E-4	0.0	0.16433586137093653	4.3910717801185445E-5
34-35	0.0	7.757560144876095E-4	0.0	0.30495261667626605	5.8547623734913925E-5
36-37	0.0	8.782143560237089E-4	0.0	0.527536045210475	5.8547623734913925E-5
38-39	0.0	8.782143560237089E-4	0.0	0.8362723020706099	5.8547623734913925E-5
40-41	0.0	9.513988856923513E-4	0.0	1.1533296399540696	5.8547623734913925E-5
42-43	0.0	9.806726975598082E-4	0.0	1.5126071330033688	5.8547623734913925E-5
44-45	0.0	0.0010831310390959077	0.0	1.9460791022307378	5.8547623734913925E-5
46-47	0.0	0.001185589380632007	0.0	2.4436460825419033	5.8547623734913925E-5
48-49	0.0	0.001185589380632007	0.0	2.952798173899616	5.8547623734913925E-5
50-51	0.0	0.0012660923632675136	0.0	3.4620819974107313	5.8547623734913925E-5
52-53	0.0	0.0012734108162343778	0.0	3.955448185718917	9.513988856923513E-5
54-55	0.0	0.0012734108162343778	0.0	4.464775919947834	1.3905060637042058E-4
56-57	0.0	0.0012880477221681064	0.0	5.026650146478836	1.4636905933728482E-4
58-59	7.318452966864241E-6	0.0012880477221681064	0.0	5.621911155444673	1.4636905933728482E-4
60-61	1.4636905933728481E-5	0.0013100030810686992	0.0	6.2205093789634	1.610059652710133E-4
62-63	1.4636905933728481E-5	0.0013173215340355634	0.0	6.781842039974854	1.610059652710133E-4
64-65	1.4636905933728481E-5	0.001551512028975219	0.0	6.927874450475663	1.610059652710133E-4
66-67	1.4636905933728481E-5	0.0015588304819420832	0.0	6.931153117404818	1.610059652710133E-4
68-69	1.4636905933728481E-5	0.001610059652710133	0.0	6.9341463646682655	1.610059652710133E-4
70-71	1.4636905933728481E-5	0.0016393334645775897	0.0	6.936510224976563	1.7564287120474178E-4
72-73	1.4636905933728481E-5	0.0016539703705113183	0.0	6.938383748936079	2.0491668307219874E-4
74-75	1.4636905933728481E-5	0.001675925729411911	0.0	6.939971853229889	2.0491668307219874E-4
76-77	1.4636905933728481E-5	0.0017125179942462322	0.0	6.941559957523699	2.0491668307219874E-4
78-79	1.4636905933728481E-5	0.0017417918061136892	0.0	6.942774820716199	2.0491668307219874E-4
80-81	1.4636905933728481E-5	0.0017417918061136892	0.0	6.943389570765415	2.0491668307219874E-4
82-83	1.4636905933728481E-5	0.0017417918061136892	0.0	6.943792085678592	2.341904949396557E-4
84-85	1.4636905933728481E-5	0.0017564287120474178	0.0	6.943967728549797	2.341904949396557E-4
86-87	1.4636905933728481E-5	0.0017710656179811461	0.0	6.944033594626498	2.341904949396557E-4
88	1.4636905933728481E-5	0.0019027977713847025	0.0	6.944040913079466	2.341904949396557E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5610	0.0	44.41681	1
GTATCAA	9510	0.0	34.56593	1
TCAACGC	10825	0.0	29.02445	4
ATCAACG	11035	0.0	28.51443	3
CAACGCA	11370	0.0	27.669432	5
AACGCAG	11720	0.0	26.877468	6
TATCAAC	12145	0.0	26.306753	2
ACGCAGA	14155	0.0	22.253408	7
GTACATG	24520	0.0	21.63286	1
TACATGG	23965	0.0	21.345478	2
CGCAGAG	15280	0.0	20.527382	8
ACATGGG	24410	0.0	20.523954	3
CATGGGG	18725	0.0	18.773878	4
GCAGAGT	18305	0.0	16.894884	9
GTGGTAT	3885	0.0	16.515396	1
TGGTATC	3645	0.0	16.15614	2
GTATAGG	1935	0.0	16.12518	1
AGAGTAC	15460	0.0	15.969053	10-11
CAGAGTA	17410	0.0	15.921459	10-11
GTATAAG	2730	0.0	14.648931	1
>>END_MODULE