FastQCFastQC Report
Fri 27 May 2016
SRR2140252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8969947
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123850.023.9199031
TGTAGAT119900.022.91002794
TCAACGC159600.022.856494
GTATCAA171550.022.74871
ATCAACG160700.022.727883
CAACGCA164650.022.1279095
AACGCAG171050.021.4994726
GTACATG276000.020.1704641
TACATGG274700.019.6850992
TATCAAC196850.019.32112
GGTGTAG140000.019.1973394
GTGTAGA127900.019.00485294
ACGCAGA197200.018.603747
ACATGGG281050.018.5956123
CGCAGAG201250.018.077398
AGGTGTA169000.017.3063394
CATGGGG211450.017.0071074
TAGGTGT190950.015.42043494
AGAGTAC183350.015.257668510-11
GGACCGA16600.015.0633556
Produced by FastQC (version 0.11.5)