##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140252_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8969947
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624772253392354	31.0	31.0	34.0	30.0	34.0
2	31.7259554599375	33.0	31.0	34.0	30.0	34.0
3	31.76459247752523	34.0	31.0	34.0	29.0	34.0
4	35.32632533949197	37.0	35.0	37.0	33.0	37.0
5	35.42019936126713	37.0	35.0	37.0	33.0	37.0
6	35.40590273275862	37.0	35.0	37.0	33.0	37.0
7	35.37784504189378	37.0	35.0	37.0	33.0	37.0
8	35.35942843363512	37.0	35.0	37.0	33.0	37.0
9	36.976386259584366	39.0	37.0	39.0	33.0	39.0
10-11	36.97684256105415	39.0	37.0	39.0	33.0	39.0
12-13	36.92994523824946	39.0	37.0	39.0	33.0	39.0
14-15	38.28874044629249	40.0	38.0	41.0	33.0	41.0
16-17	38.287985592334046	40.0	38.0	41.0	33.0	41.0
18-19	38.294433568002134	40.0	38.0	41.0	33.0	41.0
20-21	38.36155587248258	40.0	38.0	41.0	33.0	41.0
22-23	38.33332225193307	40.0	38.0	41.0	33.0	41.0
24-25	38.36044383827143	40.0	38.0	41.0	33.0	41.0
26-27	38.34972197218751	40.0	38.0	41.0	33.0	41.0
28-29	38.28640101093838	40.0	38.0	41.0	33.0	41.0
30-31	38.21739275020576	40.0	38.0	41.0	33.0	41.0
32-33	38.18211860099221	40.0	38.0	41.0	33.0	41.0
34-35	38.106865688193366	40.0	38.0	41.0	32.5	41.0
36-37	38.01873789178238	40.0	38.0	41.0	32.0	41.0
38-39	37.92746680876735	40.0	37.0	41.0	32.0	41.0
40-41	37.87825061640223	40.0	37.0	41.0	32.0	41.0
42-43	37.70962066470171	40.0	37.0	41.0	31.5	41.0
44-45	37.50451665639557	40.0	36.5	41.0	31.0	41.0
46-47	37.349770633052074	40.0	36.0	41.0	31.0	41.0
48-49	37.202738147210766	39.0	36.0	41.0	31.0	41.0
50-51	37.068697671190144	39.0	36.0	40.0	30.0	41.0
52-53	36.91871342188915	39.0	35.0	40.0	30.0	41.0
54-55	36.73746563859259	39.0	35.0	40.0	30.0	41.0
56-57	36.702179950852454	38.5	35.0	40.0	30.0	41.0
58-59	36.766984628443986	39.0	35.0	40.0	30.0	41.0
60-61	37.070429668846415	39.0	35.0	41.0	31.0	41.0
62-63	37.0297287879354	39.0	35.0	41.0	31.0	41.0
64-65	36.813038670932414	38.5	35.0	40.5	31.0	41.0
66-67	36.59344346816091	38.0	35.0	40.0	31.0	41.0
68-69	36.30909242597653	37.0	35.0	40.0	31.0	41.0
70-71	36.054263599191884	37.0	35.0	39.5	30.5	41.0
72-73	35.75211262472584	36.0	34.0	39.0	30.0	41.0
74-75	35.420055816302025	36.0	34.0	39.0	30.0	40.5
76-77	35.1011364942493	35.5	34.0	38.5	30.0	40.0
78-79	34.781137205692	35.0	34.0	37.0	30.0	39.0
80-81	34.47260945132836	35.0	34.0	37.0	30.0	39.0
82-83	34.212430366123314	35.0	34.0	36.0	29.0	38.5
84-85	33.97816057038483	35.0	34.0	36.0	29.0	37.0
86-87	33.79677701594344	35.0	33.0	35.5	29.0	37.0
88-89	33.683798832466685	35.0	33.0	35.0	29.0	36.5
90-91	33.56824275868706	35.0	33.0	35.0	29.0	36.0
92-93	33.496467672644755	35.0	33.0	35.0	29.0	36.0
94-95	33.46449614312034	35.0	33.0	35.0	29.0	36.0
96-97	33.46275553708637	35.0	33.0	35.0	29.5	36.0
98-99	33.50201359782958	35.0	33.0	35.0	30.0	35.0
100	32.77092688195211	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	2.0
16	82.0
17	432.0
18	1478.0
19	5059.0
20	12354.0
21	23988.0
22	37401.0
23	52259.0
24	68510.0
25	84001.0
26	94901.0
27	100912.0
28	114801.0
29	138077.0
30	163605.0
31	194042.0
32	243554.0
33	317005.0
34	433893.0
35	610620.0
36	967025.0
37	1721563.0
38	2325149.0
39	1208084.0
40	51149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40389012331957	20.13845789724287	14.408535524234425	24.049116455203134
2	14.216269120078351	20.589342394627447	41.97752798465444	23.216860500639772
3	18.187458511668808	25.72045152685422	30.110584627386416	25.981505334090553
4	12.576304475499663	17.782699126456272	38.80179519084301	30.839201207201057
5	13.573040317414092	37.916850727028205	35.292839384821775	13.21726957073593
6	30.33014379567692	37.37764388989349	18.96517197925496	13.327040335174626
7	26.954334598335443	32.62806752604746	22.775713297507714	17.641884578109384
8	24.521086465646547	36.80802944820732	20.99534860490478	17.67553548124135
9	25.286194970888303	17.82596170718759	21.218416991696927	35.669426330227175
10-11	24.219042948951884	26.92667548092158	28.713421088291618	20.14086048183491
12-13	24.82117573608928	24.490768922037525	28.71795600744729	21.9700993344259
14-15	22.13299944748745	26.03587184384452	26.085638107401483	25.74549060126654
16-17	22.0777428350149	29.026198376415717	26.73998738225491	22.15607140631447
18-19	22.139993930844177	28.730011306645547	27.7621905416153	21.367804220894975
20-21	23.037103138638297	27.7089195331781	27.51475620128557	21.739221126898034
22-23	22.84692911158379	27.56678029174195	27.300187721670905	22.286102875003355
24-25	22.54704548877742	27.75504500965179	27.352956030837454	22.344953470733333
26-27	22.47829397421418	27.841878365271523	27.31923464677799	22.36059301373631
28-29	22.383235077062878	27.93824484742684	27.365997475522562	22.312522599987723
30-31	22.683076635728856	27.7396729799431	27.275493610097413	22.301756774230626
32-33	22.414591008667653	27.832073861030565	27.341720936802044	22.411614193499737
34-35	22.570291754125684	27.845881229482256	27.307605318080185	22.276221698311875
36-37	22.571247390235083	27.688178353185748	27.36686442535616	22.373709831223014
38-39	22.455123626227927	27.733758093851442	27.49034235139664	22.320775928523993
40-41	22.607457871006666	27.566261921953604	27.482914747148584	22.343365459891142
42-43	22.42007123626097	27.7202867566731	27.366972903161784	22.49266910390415
44-45	22.483867959900596	27.690658007020286	27.37614549357813	22.449328539500986
46-47	22.44122086887557	27.617486781642565	27.37432408435682	22.56696826512505
48-49	22.373432937568932	27.63825489778623	27.457452803590048	22.530859361054787
50-51	22.165367221499608	27.63634230728619	27.58625771629417	22.612032754920033
52-53	22.34176339079326	27.45902805131802	27.531483182849826	22.667725375038888
54-55	22.354264055335097	27.25018652042569	27.673646337045817	22.7219030871934
56-57	22.227411158194943	27.128500244736202	27.739235227519448	22.904853369549404
58-59	22.08967848624875	27.152855435977102	27.924503722688055	22.832962355086092
60-61	22.031083936557	26.969692982429912	28.303009170514876	22.696213910498216
62-63	21.888942516533618	27.194682015710907	28.285710804352448	22.63066466340303
64-65	21.75713405768291	27.502805925518782	27.93889093011866	22.801169086679646
66-67	21.594664213701467	27.700257077079343	27.696539474942504	23.008539234276686
68-69	21.682168586817184	27.844849655719333	27.714474796345257	22.758506961118226
70-71	21.69517132279639	27.932784488824197	27.358997040483136	23.01304714789628
72-73	22.066225636549504	27.774645656641876	27.0236469331266	23.13548177368202
74-75	22.23932708810752	27.617092251497876	27.078776240795634	23.064804419598968
76-77	22.481949799500146	27.144558600045844	27.17632471288358	23.197166887570425
78-79	22.731304036603532	26.95679441265735	27.010864378646517	23.301037172092606
80-81	23.093170014319135	26.992008345121025	26.737270311228063	23.177551329331774
82-83	22.758846969329298	27.295207466596782	26.648660295745515	23.297285268328405
84-85	23.2150284152976	27.3058332951226	26.583272870471014	22.895865419108784
86-87	23.073679011887297	27.53748940048082	26.622914017355637	22.765917570276244
88-89	22.924096588645906	27.740306822328048	26.601122195694394	22.73447439333166
90-91	23.17331092623511	27.83779723575408	26.870594243083566	22.118297594927235
92-93	23.266876802316805	27.612224004991724	27.1641434912754	21.95675570141607
94-95	22.93157599044734	27.652101025538194	27.607126921416352	21.809196062598115
96-97	23.146840479355614	27.43063287526234	27.797941566589046	21.624585078792997
98-99	23.950125877862085	29.452138789801786	28.218723108669742	18.37901222366639
100	23.15687485280589	30.67481086937387	21.87420880510497	24.294105472715266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3589.0
1	2729.0
2	1944.5
3	1984.0
4	1930.5
5	2057.5
6	2395.5
7	2806.5
8	3078.0
9	3347.5
10	3772.5
11	4084.0
12	4421.5
13	4942.5
14	5637.0
15	6568.0
16	7705.0
17	9080.5
18	10905.0
19	13393.0
20	16597.0
21	20882.0
22	26623.0
23	33842.5
24	43115.0
25	54652.0
26	68655.0
27	84757.5
28	102912.5
29	123127.0
30	143142.0
31	163316.0
32	183084.5
33	201402.5
34	219989.0
35	237446.0
36	256572.5
37	276609.5
38	291812.5
39	303266.5
40	316964.0
41	330631.5
42	340897.5
43	351405.0
44	360671.0
45	371573.0
46	382119.5
47	387591.0
48	389233.0
49	385448.5
50	377820.0
51	369218.5
52	356781.0
53	339921.0
54	322916.0
55	302599.5
56	278166.0
57	251248.5
58	223696.0
59	194690.5
60	162758.0
61	132975.0
62	106485.0
63	83559.0
64	64456.5
65	49682.5
66	38624.5
67	30445.5
68	24180.5
69	19114.5
70	15279.0
71	12001.0
72	9437.0
73	7379.5
74	5719.0
75	4459.0
76	3368.0
77	2519.0
78	1904.0
79	1455.0
80	1085.0
81	769.0
82	586.0
83	427.0
84	316.0
85	248.5
86	195.5
87	145.5
88	88.5
89	56.5
90	44.5
91	38.0
92	29.5
93	17.0
94	7.0
95	4.0
96	4.0
97	3.0
98	3.0
99	8.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012296616691269191
3	0.023010169402338722
4	0.01966566803571972
5	0.01080273941417937
6	0.01392427402302377
7	0.01470465767523487
8	0.0018394757516404499
9	1.4492839255349002E-4
10-11	8.361253416547501E-5
12-13	0.00275363945851631
14-15	1.6722506833095001E-4
16-17	4.4593351554920004E-4
18-19	1.1148337888730002E-5
20-21	7.250514131168383E-5
22-23	1.6745411031742825E-5
24-25	2.7934089181367495E-5
26-27	0.002449596476518325
28-29	0.0034892939444507763
30-31	0.0026264719186377366
32-33	0.004028948558092205
34-35	0.005905216643542606
36-37	0.001065950952421506
38-39	0.0013001772917192963
40-41	5.737364673496616E-4
42-43	3.4617106816448736E-5
44-45	9.87005492104972E-5
46-47	0.0044611263414367185
48-49	6.774657056550536E-4
50-51	2.7818118982593135E-4
52-53	0.004257693420420682
54-55	2.0227036587437165E-4
56-57	1.4899296252600524E-4
58-59	6.80598112008897E-4
60-61	4.186628661647856E-5
62-63	0.0
64-65	0.0
66-67	0.001730367105024295
68-69	0.0
70-71	4.243464269375611E-4
72-73	0.0015951075969426643
74-75	1.2101984056591425E-4
76-77	1.2873802937479985E-5
78-79	2.6038984786853332E-5
80-81	0.003115622051727362
82-83	1.3329477559791204E-4
84-85	3.438953948428233E-4
86-87	0.01330734829042885
88-89	7.533684555089618E-4
90-91	8.968738062119162E-4
92-93	2.1329714297008876E-5
94-95	1.7342169457708193E-4
96-97	7.390660171458882E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3347.0
20-21	6925.0
22-23	7925.0
24-25	8798.0
26-27	12047.0
28-29	15934.0
30-31	23099.0
32-33	27012.0
34-35	35289.0
36-37	48063.0
38-39	53575.0
40-41	50067.0
42-43	51274.0
44-45	58729.0
46-47	64496.0
48-49	39729.0
50-51	39646.0
52-53	15612.0
54-55	15107.0
56-57	14582.0
58-59	14847.0
60-61	16083.0
62-63	72622.0
64-65	77395.0
66-67	81561.0
68-69	82833.0
70-71	82932.0
72-73	80434.0
74-75	81293.0
76-77	85207.0
78-79	90153.0
80-81	89103.0
82-83	87475.0
84-85	87713.0
86-87	91248.0
88-89	96505.0
90-91	102542.0
92-93	109058.0
94-95	132186.0
96-97	323684.0
98-99	608731.0
100-101	5885086.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.73096060579878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23440605862723	72.99717171712342
2	7.89751621908654	13.06738206409811
3	2.1112312156232527	5.2399255958838005
4	0.8188936278647656	2.7099142586887828
5	0.36107299233094364	1.4935957752174591
6	0.1923147126166685	0.9546228548043068
7	0.11438338666353787	0.6624133219213301
8	0.0688505059541543	0.4556854796625963
9	0.05057225794267775	0.37655023316417713
>10	0.14828462101977852	1.8646681657169744
>50	0.001982315297324857	0.11306218693194241
>100	4.920869731730195E-4	0.0650083467870578
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.344501366619E-5	0.0	0.0	0.0
3	0.0	3.344501366619E-5	0.0	0.0	0.0
4	0.0	1.0033504099857E-4	0.0	0.0	1.114833788873E-5
5	0.0	1.1148337888730001E-4	0.0	0.0	1.114833788873E-5
6	0.0	1.1148337888730001E-4	0.0	0.0	3.344501366619E-5
7	0.0	1.4492839255349E-4	0.0	0.0	3.344501366619E-5
8	2.229667577746E-5	1.6722506833095001E-4	0.0	1.114833788873E-5	3.344501366619E-5
9	2.229667577746E-5	2.2296675777460002E-4	0.0	2.229667577746E-5	3.344501366619E-5
10-11	2.229667577746E-5	2.2296675777460002E-4	0.0	7.803836522111E-5	3.344501366619E-5
12-13	2.229667577746E-5	2.2296675777460002E-4	0.0	1.83947575164045E-4	3.344501366619E-5
14-15	2.229667577746E-5	2.6756010932952E-4	0.0	3.1215346088444E-4	4.459335155492E-5
16-17	2.229667577746E-5	3.0100512299571E-4	0.0	5.2397188077031E-4	4.459335155492E-5
18-19	2.229667577746E-5	3.6789515032809E-4	0.0	8.1382866587729E-4	4.459335155492E-5
20-21	2.229667577746E-5	4.2363683977174E-4	0.0	0.001321078039814505	4.459335155492E-5
22-23	2.229667577746E-5	4.4035934660483503E-4	0.0	0.002079165016248145	4.459335155492E-5
24-25	2.229667577746E-5	4.73804360271025E-4	0.0	0.0029320128647359897	4.459335155492E-5
26-27	2.229667577746E-5	4.9052686710412E-4	0.0	0.003929789105777325	4.459335155492E-5
28-29	2.229667577746E-5	5.0167520499285E-4	0.0	0.007909745732053934	4.459335155492E-5
30-31	2.229667577746E-5	5.4626855654777E-4	0.0	0.018355738333793947	5.0167520499285E-5
32-33	2.229667577746E-5	5.51842725492135E-4	0.0	0.04775947951531932	1.1148337888730001E-4
34-35	2.229667577746E-5	6.2430692176888E-4	0.0	0.08738624654081012	1.2263171677603E-4
36-37	2.229667577746E-5	6.800486112125301E-4	0.0	0.14378568791989518	1.2263171677603E-4
38-39	2.229667577746E-5	6.9119694910126E-4	0.0	0.21169021400014962	1.2263171677603E-4
40-41	2.229667577746E-5	7.30216131711815E-4	0.0	0.27330707751116035	1.28205885720395E-4
42-43	2.229667577746E-5	7.52512807489275E-4	0.0	0.38168007012750466	1.3378005466476E-4
44-45	2.229667577746E-5	7.803836522111E-4	0.0	0.48882674557608874	1.3935422360912498E-4
46-47	2.229667577746E-5	7.9153199009983E-4	0.0	0.609524225728424	1.5607673044222E-4
48-49	2.229667577746E-5	7.97106159044195E-4	0.0	0.6902660628875511	1.6722506833095001E-4
50-51	2.229667577746E-5	9.2531204476459E-4	0.0	1.035251378854301	1.6722506833095001E-4
52-53	2.229667577746E-5	9.4760872054205E-4	0.0	1.4921771555617886	1.83947575164045E-4
54-55	2.229667577746E-5	9.4760872054205E-4	0.0	2.0990480768726947	2.0067008199714E-4
56-57	2.229667577746E-5	9.6990539631951E-4	0.0	2.7572961133438136	2.06244250941505E-4
58-59	2.229667577746E-5	0.0010144987478744299	0.0	3.4384762808520497	2.1181841988587E-4
60-61	2.229667577746E-5	0.001042369592596255	0.0	4.111356510802126	2.2296675777460002E-4
62-63	2.229667577746E-5	0.00105909209942935	0.0	4.599213350981895	2.5641177144079E-4
64-65	2.229667577746E-5	0.0011371304646504601	0.0	4.607730681128885	2.5641177144079E-4
66-67	2.229667577746E-5	0.001142704633594825	0.0	4.610874512413507	2.5641177144079E-4
68-69	2.229667577746E-5	0.00114827880253919	0.0	4.613834396122964	2.5641177144079E-4
70-71	2.229667577746E-5	0.001153852971483555	0.0	4.6163539204858175	2.6756010932952E-4
72-73	2.229667577746E-5	0.001176149647261015	0.0	4.618789832314505	3.1215346088444E-4
74-75	2.229667577746E-5	0.001198446323038475	0.0	4.620919164851253	3.1215346088444E-4
76-77	2.229667577746E-5	0.001254188012482125	0.0	4.622892420657559	3.1215346088444E-4
78-79	2.229667577746E-5	0.00128205885720395	0.0	4.624180053683706	3.1215346088444E-4
80-81	2.229667577746E-5	0.00128205885720395	0.0	4.62480993477442	3.3445013666190003E-4
82-83	2.229667577746E-5	0.00129320719509268	0.0	4.625294887472579	3.5674681243936E-4
84-85	2.229667577746E-5	0.001298781364037045	0.0	4.625411945020411	3.73469319272455E-4
86-87	2.229667577746E-5	0.001354523053480695	0.0	4.625478835047743	3.7904348821682E-4
88	2.229667577746E-5	0.00142698724975744	0.0	4.625478835047743	3.7904348821682E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12385	0.0	23.919903	1
TGTAGAT	11990	0.0	22.910027	94
TCAACGC	15960	0.0	22.85649	4
GTATCAA	17155	0.0	22.7487	1
ATCAACG	16070	0.0	22.72788	3
CAACGCA	16465	0.0	22.127909	5
AACGCAG	17105	0.0	21.499472	6
GTACATG	27600	0.0	20.170464	1
TACATGG	27470	0.0	19.685099	2
TATCAAC	19685	0.0	19.3211	2
GGTGTAG	14000	0.0	19.19733	94
GTGTAGA	12790	0.0	19.004852	94
ACGCAGA	19720	0.0	18.60374	7
ACATGGG	28105	0.0	18.595612	3
CGCAGAG	20125	0.0	18.07739	8
AGGTGTA	16900	0.0	17.30633	94
CATGGGG	21145	0.0	17.007107	4
TAGGTGT	19095	0.0	15.420434	94
AGAGTAC	18335	0.0	15.2576685	10-11
GGACCGA	1660	0.0	15.063355	6
>>END_MODULE