FastQCFastQC Report
Fri 27 May 2016
SRR2140251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8530039
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66850.038.9820561
GTATCAA106150.031.4401171
TCAACGC113050.027.7760034
ATCAACG114800.027.3541933
CAACGCA116300.026.9597765
AACGCAG124250.025.4083236
TATCAAC131150.024.5786882
GTACATG265450.022.059611
TACATGG266550.021.4263442
ACGCAGA151000.020.847847
ACATGGG275450.020.1297113
CGCAGAG160450.019.5907298
CATGGGG206300.018.1504764
GTATAGG27750.015.7828771
GCAGAGT202600.015.3851359
AGAGTAC169350.015.12286210-11
GTATTAG26750.014.8993321
GTATAAG36950.014.6979381
CAGAGTA193550.014.32902710-11
ATGGGGG133400.014.2139585
Produced by FastQC (version 0.11.5)