FastQCFastQC Report
Fri 27 May 2016
SRR2140250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8251620
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63000.036.7598761
GTATCAA103750.031.7701971
TCAACGC109500.028.7318924
ATCAACG111550.028.2431033
CAACGCA113800.027.6815055
AACGCAG120200.026.4535056
TATCAAC128150.025.3202462
ACGCAGA143600.021.9944937
CGCAGAG150100.021.0110998
GTACATG242650.019.6703411
TACATGG241700.019.0215072
ACATGGG247050.017.7773363
GCAGAGT189800.016.5033329
CATGGGG186650.015.8009144
AGAGTAC160100.015.67593410-11
CCGTCGA3751.3082172E-814.8343619
CAGAGTA183600.014.57845110-11
TATACCG5801.2732926E-1113.2830755
GTGTAGC45250.013.2442311
ATGGGGG114800.012.7508285
Produced by FastQC (version 0.11.5)