##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8251620
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.631977599550147	31.0	31.0	34.0	30.0	34.0
2	31.73737120710842	33.0	31.0	34.0	30.0	34.0
3	31.777153577115765	34.0	31.0	34.0	30.0	34.0
4	35.358727983111194	37.0	35.0	37.0	33.0	37.0
5	35.450121915454176	37.0	35.0	37.0	33.0	37.0
6	35.43432332075398	37.0	35.0	37.0	33.0	37.0
7	35.40691149131928	37.0	35.0	37.0	33.0	37.0
8	35.386024562449556	37.0	35.0	37.0	33.0	37.0
9	37.023318451407114	39.0	37.0	39.0	33.0	39.0
10-11	37.027218594651714	39.0	37.0	39.0	33.0	39.0
12-13	36.98594930450021	39.0	37.0	39.0	33.0	39.0
14-15	38.35166179489603	40.0	38.0	41.0	33.0	41.0
16-17	38.345655095605466	40.0	38.0	41.0	33.0	41.0
18-19	38.347149771802385	40.0	38.0	41.0	33.0	41.0
20-21	38.40871654110823	40.0	38.0	41.0	33.0	41.0
22-23	38.381345141099416	40.0	38.0	41.0	33.0	41.0
24-25	38.407815822912134	40.0	38.0	41.0	33.0	41.0
26-27	38.39805441261868	40.0	38.0	41.0	33.0	41.0
28-29	38.333373626919666	40.0	38.0	41.0	33.0	41.0
30-31	38.26438660385965	40.0	38.0	41.0	33.0	41.0
32-33	38.23148545224643	40.0	38.0	41.0	33.0	41.0
34-35	38.15603250827584	40.0	38.0	41.0	33.0	41.0
36-37	38.06429403265529	40.0	38.0	41.0	32.0	41.0
38-39	37.97744207285136	40.0	38.0	41.0	32.0	41.0
40-41	37.929525018999534	40.0	37.0	41.0	32.0	41.0
42-43	37.75692405586854	40.0	37.0	41.0	32.0	41.0
44-45	37.546122025778956	40.0	37.0	41.0	31.0	41.0
46-47	37.3934073284824	40.0	36.0	41.0	31.0	41.0
48-49	37.253348714698575	39.0	36.0	41.0	31.0	41.0
50-51	37.111473892971915	39.0	36.0	40.0	30.5	41.0
52-53	36.956465276997704	39.0	35.5	40.0	30.0	41.0
54-55	36.77426731347181	39.0	35.0	40.0	30.0	41.0
56-57	36.73968975373921	39.0	35.0	40.0	30.0	41.0
58-59	36.797748608165556	39.0	35.0	40.0	30.0	41.0
60-61	37.0990286015834	39.0	35.0	41.0	31.0	41.0
62-63	37.051277528505466	39.0	35.0	41.0	31.0	41.0
64-65	36.82961886308618	38.5	35.0	40.5	31.0	41.0
66-67	36.60306891991364	38.0	35.0	40.0	31.0	41.0
68-69	36.30122717836556	37.0	35.0	40.0	31.0	41.0
70-71	36.04213354211579	37.0	34.5	39.5	30.5	41.0
72-73	35.73339049379844	36.0	34.0	39.0	30.0	41.0
74-75	35.41925481277533	36.0	34.0	39.0	30.0	40.5
76-77	35.116722402218336	35.5	34.0	38.5	30.0	40.0
78-79	34.81712764542626	35.0	34.0	37.0	30.0	39.0
80-81	34.527329523166316	35.0	34.0	37.0	30.0	39.0
82-83	34.29016159261597	35.0	34.0	36.5	30.0	39.0
84-85	34.07233298671576	35.0	34.0	36.0	30.0	37.0
86-87	33.88918103596944	35.0	34.0	36.0	29.5	37.0
88-89	33.75148319746333	35.0	34.0	35.0	29.5	37.0
90-91	33.63815914337238	35.0	34.0	35.0	29.5	36.0
92-93	33.580365450033426	35.0	34.0	35.0	29.5	36.0
94-95	33.561409079611124	35.0	34.0	35.0	30.0	36.0
96-97	33.56875351002502	35.0	34.0	35.0	30.0	36.0
98-99	33.60715314609689	35.0	34.0	35.0	30.0	35.5
100	32.896267322756536	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	22.0
17	228.0
18	1190.0
19	3984.0
20	10309.0
21	20432.0
22	32467.0
23	45809.0
24	60226.0
25	75337.0
26	86235.0
27	91175.0
28	103051.0
29	124373.0
30	147097.0
31	172852.0
32	217946.0
33	282460.0
34	384427.0
35	546279.0
36	865916.0
37	1555211.0
38	2128470.0
39	1242460.0
40	53662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30096877946391	20.232039284407183	14.321890731759341	24.145101204369563
2	14.152223116161124	20.625004696613324	42.22541416857339	22.99735801865216
3	18.10335746858968	25.879465298189995	30.05615801774525	25.961019215475073
4	12.485083371302935	17.810912238890058	39.051718777443114	30.652285612363894
5	13.588829270261455	37.80019748443086	35.40367167843707	13.20730156687061
6	30.163016964687312	37.60698605695104	19.07652526376915	13.153471714592502
7	26.799063024748683	32.538484903358366	23.020993704773286	17.641458367119665
8	24.290410596328265	36.995734816448284	21.191008332826353	17.522846254397102
9	25.141245128830587	17.800461291684584	21.38900859613855	35.66928498334628
10-11	23.988397660966125	27.030694123766363	28.980422249616193	20.00048596565132
12-13	24.58152512164205	24.510567134890408	28.927850270528083	21.980057472939464
14-15	21.95724938452849	26.07866801113311	26.230650647713738	25.73343195662466
16-17	21.98781784231279	29.115456247388376	26.88033776820331	22.016388142095522
18-19	22.040056624039476	28.722690662280943	27.934015130848866	21.303237582830715
20-21	22.838180366499607	27.859614173290403	27.68643410688228	21.615771353327712
22-23	22.646023876433976	27.722371753439194	27.464050728473783	22.167553641653047
24-25	22.358165782549495	27.856311889897068	27.542313735987882	22.24320859156555
26-27	22.329963089503487	27.978945203586296	27.497608845975403	22.19348286093481
28-29	22.272438007721416	28.00971775540246	27.52172473121656	22.196119505659574
30-31	22.514096949720802	27.82900423601647	27.454328685993968	22.20257012826876
32-33	22.29500683464684	27.90079680878488	27.496608619730967	22.307587736837313
34-35	22.431805401346327	27.85571142284569	27.531360758818085	22.181122416989897
36-37	22.409792816296324	27.657884074095445	27.5886218929451	22.343701216663128
38-39	22.24221247536655	27.70674938806865	27.75424504768802	22.296793088876775
40-41	22.430296467167576	27.542055762826266	27.770945673646292	22.256702096359867
42-43	22.194617359948058	27.627310911259656	27.737367818658075	22.44070391013421
44-45	22.252752373097614	27.68640584290571	27.693306294202703	22.367535489793973
46-47	22.11740180604755	27.6392125861913	27.688845882282937	22.554539725478215
48-49	22.008152927791418	27.660000923916023	27.801958348553125	22.529887799739434
50-51	21.90742959650733	27.780678542769692	27.826154437433782	22.485737423289194
52-53	22.047303104345286	27.750575709321673	27.669768124142124	22.532353062190918
54-55	22.088931796941583	27.64671905129541	27.669382509802187	22.594966641960816
56-57	22.086125973566663	27.66152368173813	27.649069851738545	22.603280492956657
58-59	22.14618979248632	27.668997856225047	27.621047143523867	22.563765207764764
60-61	22.234871230866965	27.56566644788171	27.636571745964577	22.56289057528675
62-63	22.22547808138997	27.72894321256687	27.463889697870226	22.581689008172937
64-65	22.30825995041411	27.73604501011234	27.35336707308431	22.60232796638924
66-67	22.323815625280506	27.7492598694565	27.23393378216151	22.692990723101484
68-69	22.356299416550037	27.695658165273322	27.277138903354796	22.670903514821845
70-71	22.34710434896258	27.548908230574437	27.410117802186072	22.693869618276903
72-73	22.465318883496938	27.54987742344415	27.353045074412297	22.631758618646618
74-75	22.415998401759197	27.72266792054347	27.373158709889623	22.488174967807712
76-77	22.497144360146144	27.595901205048918	27.40082330794089	22.506131126864048
78-79	22.47320233217227	27.535911338043274	27.401647705209715	22.589238624574744
80-81	22.479317834765286	27.671611186681126	27.390338882250237	22.458732096303347
82-83	22.462669012006135	27.66835872967301	27.50860023190197	22.360372026418887
84-85	22.500842700745974	27.74958786607623	27.502511482411464	22.247057950766333
86-87	22.300104777372994	27.830571845315795	27.57077454775955	22.298548829551663
88-89	22.330452172644428	27.81087365068562	27.546917171542475	22.311757005127483
90-91	22.35328718657696	27.80961344261803	27.577390036943193	22.259709333861807
92-93	22.368153628659623	27.77194233952487	27.589665991384905	22.270238040430606
94-95	22.18412179995206	27.89509770759468	27.626542761156365	22.294237731296896
96-97	22.23523543370685	28.030488148483627	27.677236364547092	22.057040053262426
98-99	23.28333150533166	29.96849264193736	27.876746828250504	18.87142902448047
100	22.268378966658457	30.977856966913404	21.582398287061043	25.1713657793671
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2796.0
1	2075.0
2	1476.5
3	1550.0
4	1475.5
5	1536.5
6	1813.5
7	2154.0
8	2387.0
9	2636.5
10	2973.0
11	3241.5
12	3507.0
13	3947.0
14	4589.5
15	5386.5
16	6356.5
17	7628.5
18	9221.0
19	11450.5
20	14591.0
21	18758.0
22	24450.0
23	31717.5
24	40823.5
25	52743.5
26	67144.0
27	83005.0
28	100998.0
29	120787.0
30	140223.0
31	159573.0
32	178960.0
33	196195.0
34	213756.0
35	231446.0
36	249219.5
37	266479.5
38	280457.0
39	293052.0
40	306177.5
41	316560.0
42	324256.5
43	331497.0
44	337264.0
45	343898.0
46	348014.0
47	348744.5
48	348009.0
49	343676.5
50	337243.0
51	327862.0
52	315398.5
53	300523.5
54	284304.0
55	266854.5
56	246280.5
57	223258.5
58	198294.0
59	171759.0
60	144098.0
61	118409.0
62	96121.5
63	76020.0
64	57939.0
65	44040.5
66	34232.0
67	27214.5
68	21714.0
69	16968.0
70	13361.0
71	10375.0
72	7937.5
73	6121.0
74	4711.0
75	3613.5
76	2733.5
77	2015.0
78	1498.0
79	1120.0
80	837.0
81	642.5
82	502.0
83	343.0
84	234.5
85	180.0
86	147.0
87	118.0
88	79.0
89	49.0
90	34.5
91	31.0
92	28.5
93	20.0
94	14.0
95	11.5
96	10.5
97	10.0
98	6.0
99	5.5
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012046119428669766
3	0.0225773848044384
4	0.01937801304471122
5	0.010216175732765202
6	0.013682161805802983
7	0.0150515898696256
8	0.001914775522867025
9	9.695065938567213E-5
10-11	9.695065938567213E-5
12-13	0.003132718181399531
14-15	2.3631723225257586E-4
16-17	5.332286266211968E-4
18-19	1.2118832423209017E-5
20-21	8.487428663162086E-5
22-23	0.0
24-25	4.857172711407987E-5
26-27	0.002236434112767085
28-29	0.003681016831920999
30-31	0.0026323972419057895
32-33	0.004028656443686433
34-35	0.005908724880655685
36-37	0.0011713229718880033
38-39	0.001360341885308283
40-41	6.92271138870085E-4
42-43	4.342227580117511E-5
44-45	6.856812305360032E-5
46-47	0.004257146708548463
48-49	6.878835621387625E-4
50-51	3.0482283062036785E-4
52-53	0.004350693494793255
54-55	1.7359214679414845E-4
56-57	2.5257313740911254E-4
58-59	6.530083014986345E-4
60-61	1.3174524106546592E-5
62-63	6.64461010491042E-6
64-65	0.0
66-67	0.0016584714484663167
68-69	0.0
70-71	3.801326898078547E-4
72-73	0.0014252884741951521
74-75	1.836730224596785E-4
76-77	2.8597499277376936E-5
78-79	1.4489139737755263E-5
80-81	0.003056176116850543
82-83	9.683678182769891E-5
84-85	3.248410073244093E-4
86-87	0.013534147589343
88-89	5.36588867243015E-4
90-91	9.632457021871678E-4
92-93	4.812362767455082E-5
94-95	2.1212181535807631E-4
96-97	9.183168821038071E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2798.0
20-21	5506.0
22-23	6308.0
24-25	7458.0
26-27	9191.0
28-29	11053.0
30-31	14431.0
32-33	15675.0
34-35	20011.0
36-37	28066.0
38-39	33133.0
40-41	30241.0
42-43	35254.0
44-45	44953.0
46-47	51647.0
48-49	49337.0
50-51	48198.0
52-53	48486.0
54-55	53816.0
56-57	62556.0
58-59	66386.0
60-61	65882.0
62-63	66232.0
64-65	70025.0
66-67	74833.0
68-69	76408.0
70-71	78010.0
72-73	77963.0
74-75	82122.0
76-77	89929.0
78-79	96140.0
80-81	94242.0
82-83	93358.0
84-85	94083.0
86-87	94496.0
88-89	95985.0
90-91	98972.0
92-93	101992.0
94-95	120798.0
96-97	290127.0
98-99	528754.0
100-101	5216765.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.28673926052518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.08446683917394	77.73094901575229
2	7.122014739621181	12.290708576946201
3	1.6693774539729689	4.321354112950948
4	0.5526028596192267	1.9072919545033924
5	0.23719136331043036	1.0233234660407802
6	0.11743618247904239	0.6079911154392352
7	0.06296821575892063	0.3803325410423333
8	0.03824530707118188	0.2640050271351832
9	0.02948393200709138	0.2289665118123852
>10	0.08442783773576469	1.1215167986047376
>50	0.0015317906782183835	0.08576036437739343
>100	2.534785721072699E-4	0.037800515395162465
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2118832423209018E-5
2	0.0	0.0	0.0	3.6356497269627055E-5	1.2118832423209018E-5
3	0.0	0.0	0.0	3.6356497269627055E-5	1.2118832423209018E-5
4	0.0	3.6356497269627055E-5	0.0	6.059416211604509E-5	1.2118832423209018E-5
5	0.0	6.059416211604509E-5	0.0	8.483182696246312E-5	1.2118832423209018E-5
6	0.0	6.059416211604509E-5	0.0	8.483182696246312E-5	1.2118832423209018E-5
7	0.0	6.059416211604509E-5	0.0	9.695065938567215E-5	1.2118832423209018E-5
8	1.2118832423209018E-5	8.483182696246312E-5	0.0	1.0906949180888116E-4	1.2118832423209018E-5
9	1.2118832423209018E-5	1.6966365392492624E-4	0.0	1.4542598907850822E-4	1.2118832423209018E-5
10-11	1.2118832423209018E-5	1.6966365392492624E-4	0.0	1.878419025597398E-4	1.2118832423209018E-5
12-13	1.2118832423209018E-5	1.6966365392492624E-4	0.0	2.2419839982936682E-4	1.2118832423209018E-5
14-15	1.2118832423209018E-5	2.3025781604097136E-4	0.0	2.666143133105984E-4	1.2118832423209018E-5
16-17	1.2118832423209018E-5	2.3025781604097136E-4	0.0	3.5144614027306155E-4	1.2118832423209018E-5
18-19	1.2118832423209018E-5	2.544954808873894E-4	0.0	4.0598088617750216E-4	2.4237664846418037E-5
20-21	1.2118832423209018E-5	2.666143133105984E-4	0.0	5.211097941979878E-4	3.6356497269627055E-5
22-23	1.2118832423209018E-5	3.2114905921503897E-4	0.0	7.695458588737726E-4	3.6356497269627055E-5
24-25	1.2118832423209018E-5	3.45386724061457E-4	0.0	0.001320952734129783	3.6356497269627055E-5
26-27	1.2118832423209018E-5	3.6356497269627057E-4	0.0	0.002914579197781769	3.6356497269627055E-5
28-29	1.2118832423209018E-5	3.6356497269627057E-4	0.0	0.01463349015102489	3.6356497269627055E-5
30-31	1.2118832423209018E-5	3.878026375426886E-4	0.0	0.053456169818774976	4.8475329692836074E-5
32-33	1.2118832423209018E-5	3.878026375426886E-4	0.0	0.156399591837724	6.059416211604509E-5
34-35	1.2118832423209018E-5	4.483967996587337E-4	0.0	0.29689927553619777	6.059416211604509E-5
36-37	1.2118832423209018E-5	5.150503779863833E-4	0.0	0.5002108676841639	6.059416211604509E-5
38-39	1.2118832423209018E-5	5.211097941979878E-4	0.0	0.7432540519316206	6.059416211604509E-5
40-41	1.2118832423209018E-5	6.059416211604509E-4	0.0	0.9638713367799293	6.059416211604509E-5
42-43	1.2118832423209018E-5	6.241198697952645E-4	0.0	1.2449858330848973	6.059416211604509E-5
44-45	1.2118832423209018E-5	6.90773448122914E-4	0.0	1.4689176186009534	6.059416211604509E-5
46-47	1.2118832423209018E-5	7.513676102389592E-4	0.0	1.682518099476224	6.059416211604509E-5
48-49	1.2118832423209018E-5	7.574270264505637E-4	0.0	1.8356759036407397	6.66535783276496E-5
50-51	1.2118832423209018E-5	8.604371020478403E-4	0.0	2.010362813605086	7.271299453925411E-5
52-53	1.2118832423209018E-5	8.725559344710493E-4	0.0	2.1823472239390567	7.271299453925411E-5
54-55	1.2118832423209018E-5	8.967935993174674E-4	0.0	2.345866629825416	7.271299453925411E-5
56-57	1.2118832423209018E-5	9.089124317406764E-4	0.0	2.4754775425916367	8.483182696246314E-5
58-59	1.2118832423209018E-5	9.270906803754899E-4	0.0	2.5345932071520503	9.695065938567215E-5
60-61	1.2118832423209018E-5	9.452689290103034E-4	0.0	2.5565464720866933	9.695065938567215E-5
62-63	1.2118832423209018E-5	9.573877614335125E-4	0.0	2.568738017504441	9.695065938567215E-5
64-65	1.2118832423209018E-5	0.0010543384208191846	0.0	2.572828123447275	9.695065938567215E-5
66-67	1.2118832423209018E-5	0.0011028137505120206	0.0	2.5762213965257734	9.695065938567215E-5
68-69	1.2118832423209018E-5	0.0011270514153584386	0.0	2.5792208075505174	1.0301007559727665E-4
70-71	1.2118832423209018E-5	0.0011391702477816477	0.0	2.581771821775603	1.0906949180888116E-4
72-73	1.2118832423209018E-5	0.0011391702477816477	0.0	2.5841471129305518	1.0906949180888116E-4
74-75	1.2118832423209018E-5	0.001145229663993252	0.0	2.5864496910909613	1.0906949180888116E-4
76-77	1.2118832423209018E-5	0.0011573484964164612	0.0	2.5884856549380606	1.0906949180888116E-4
78-79	1.2118832423209018E-5	0.0011634079126280658	0.0	2.5899459742450572	1.0906949180888116E-4
80-81	1.2118832423209018E-5	0.0011634079126280658	0.0	2.5907094606877195	1.0906949180888116E-4
82-83	1.2118832423209018E-5	0.0011634079126280658	0.0	2.591145738654955	1.0906949180888116E-4
84-85	1.2118832423209018E-5	0.0011634079126280658	0.0	2.5912487487305524	1.0906949180888116E-4
86-87	1.2118832423209018E-5	0.0011755267450512749	0.0	2.5913335805575146	1.0906949180888116E-4
88	1.2118832423209018E-5	0.001272477404436947	0.0	2.591345699389938	1.0906949180888116E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6300	0.0	36.759876	1
GTATCAA	10375	0.0	31.770197	1
TCAACGC	10950	0.0	28.731892	4
ATCAACG	11155	0.0	28.243103	3
CAACGCA	11380	0.0	27.681505	5
AACGCAG	12020	0.0	26.453505	6
TATCAAC	12815	0.0	25.320246	2
ACGCAGA	14360	0.0	21.994493	7
CGCAGAG	15010	0.0	21.011099	8
GTACATG	24265	0.0	19.670341	1
TACATGG	24170	0.0	19.021507	2
ACATGGG	24705	0.0	17.777336	3
GCAGAGT	18980	0.0	16.503332	9
CATGGGG	18665	0.0	15.800914	4
AGAGTAC	16010	0.0	15.675934	10-11
CCGTCGA	375	1.3082172E-8	14.834361	9
CAGAGTA	18360	0.0	14.578451	10-11
TATACCG	580	1.2732926E-11	13.283075	5
GTGTAGC	4525	0.0	13.244231	1
ATGGGGG	11480	0.0	12.750828	5
>>END_MODULE