FastQCFastQC Report
Fri 27 May 2016
SRR2140249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6985697
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55550.039.106071
GTATCAA90350.032.41511
TCAACGC96900.027.9079384
ATCAACG99750.027.243793
CAACGCA100600.026.835495
AACGCAG104050.025.942546
TATCAAC108700.025.7698082
GTACATG226150.021.2337821
ACGCAGA127950.021.0954827
TACATGG225700.020.9254132
ACATGGG226700.019.9791813
CGCAGAG138550.019.416418
CATGGGG173750.017.8418854
GTGGTAT40750.017.2660771
TGGTATC39100.016.3076932
GCAGAGT167150.016.225339
AGAGTAC145200.015.24219710-11
GTGTAGC37700.014.8137051
GTATAGG22200.014.6581791
CAGAGTA163500.014.55778910-11
Produced by FastQC (version 0.11.5)