##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6985697
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50229805272115	31.0	31.0	34.0	28.0	34.0
2	31.617612673438312	33.0	31.0	34.0	28.0	34.0
3	31.656258781335634	33.0	31.0	34.0	28.0	34.0
4	35.24221434167557	37.0	35.0	37.0	32.0	37.0
5	35.340422294296474	37.0	35.0	37.0	33.0	37.0
6	35.32246603309591	37.0	35.0	37.0	32.0	37.0
7	35.29761282231394	37.0	35.0	37.0	32.0	37.0
8	35.27359202667966	37.0	35.0	37.0	32.0	37.0
9	36.89948189851349	39.0	37.0	39.0	33.0	39.0
10-11	36.90464580699678	39.0	37.0	39.0	33.0	39.0
12-13	36.86063366332665	39.0	37.0	39.0	33.0	39.0
14-15	38.20388595440083	40.0	38.0	41.0	33.0	41.0
16-17	38.19787860824768	40.0	38.0	41.0	33.0	41.0
18-19	38.195817754477474	40.0	38.0	41.0	33.0	41.0
20-21	38.261642203538955	40.0	38.0	41.0	33.0	41.0
22-23	38.22841493933711	40.0	38.0	41.0	33.0	41.0
24-25	38.255257723124004	40.0	38.0	41.0	33.0	41.0
26-27	38.24442527332184	40.0	38.0	41.0	33.0	41.0
28-29	38.179530630676766	40.0	38.0	41.0	32.0	41.0
30-31	38.111883540955326	40.0	38.0	41.0	32.0	41.0
32-33	38.07914146942182	40.0	38.0	41.0	32.0	41.0
34-35	38.003687143511506	40.0	38.0	41.0	32.0	41.0
36-37	37.911216155764784	40.0	37.0	41.0	32.0	41.0
38-39	37.81843650430264	40.0	37.0	41.0	32.0	41.0
40-41	37.76799547802189	40.0	37.0	41.0	32.0	41.0
42-43	37.59177160707387	40.0	37.0	41.0	31.0	41.0
44-45	37.37704472703467	40.0	36.0	41.0	31.0	41.0
46-47	37.22795085594045	39.0	36.0	41.0	31.0	41.0
48-49	37.0842429512486	39.0	36.0	40.5	30.0	41.0
50-51	36.94513320837703	39.0	35.0	40.0	30.0	41.0
52-53	36.79360717540811	39.0	35.0	40.0	30.0	41.0
54-55	36.609140792582544	38.5	35.0	40.0	30.0	41.0
56-57	36.58706353053474	38.5	35.0	40.0	30.0	41.0
58-59	36.65138045110493	39.0	35.0	40.0	30.0	41.0
60-61	36.9634286619454	39.0	35.0	40.5	31.0	41.0
62-63	36.92370922892756	39.0	35.0	41.0	31.0	41.0
64-65	36.69637815433853	38.0	35.0	40.0	31.0	41.0
66-67	36.45894993222669	38.0	35.0	40.0	31.0	41.0
68-69	36.15490501973582	37.0	35.0	40.0	30.0	41.0
70-71	35.88064610443337	37.0	34.0	39.5	30.0	41.0
72-73	35.55336638721962	36.0	34.0	39.0	30.0	41.0
74-75	35.221221469147835	36.0	34.0	39.0	30.0	40.5
76-77	34.89246592839258	35.0	34.0	37.5	29.5	40.0
78-79	34.56788064658534	35.0	34.0	37.0	29.0	39.0
80-81	34.25117520097922	35.0	34.0	37.0	29.0	39.0
82-83	33.99637826421734	35.0	33.0	36.0	29.0	38.5
84-85	33.771279864829225	35.0	33.0	36.0	29.0	37.0
86-87	33.58092384438176	35.0	33.0	35.5	29.0	37.0
88-89	33.43310516512392	35.0	33.0	35.0	29.0	36.5
90-91	33.298252836479634	35.0	33.0	35.0	29.0	36.0
92-93	33.220449488175035	35.0	33.0	35.0	29.0	36.0
94-95	33.21445944411638	35.0	33.0	35.0	29.0	36.0
96-97	33.25138798076712	35.0	33.0	35.0	29.0	35.5
98-99	33.37403517714051	35.0	33.0	35.0	29.0	35.0
100	32.63230164866969	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	17.0
17	251.0
18	1148.0
19	3915.0
20	9759.0
21	19327.0
22	30759.0
23	43986.0
24	57243.0
25	70842.0
26	79848.0
27	84627.0
28	96627.0
29	114962.0
30	134434.0
31	159807.0
32	200781.0
33	262023.0
34	355391.0
35	508761.0
36	817239.0
37	1404364.0
38	1738723.0
39	756868.0
40	33993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43104116883398	20.073744967753395	14.27917643722595	24.21603742618668
2	14.340536964464698	20.62063690065523	41.898194909358914	23.14063122552115
3	18.327632575375215	25.583395223390532	29.83599027456276	26.252981926671488
4	12.65332595496955	17.669243605557252	38.52698555420413	31.150444885269067
5	13.775357033183274	37.642804325557854	35.20168589956042	13.380152741698456
6	30.5547482458313	37.15043687784457	18.90738304229619	13.387431834027938
7	27.153211226162842	32.36797827933232	22.697805655918003	17.781004838586828
8	24.546464680589857	36.801208893082936	20.934243820541905	17.718082605785302
9	25.288110408563792	17.780892080810915	21.246591245818237	35.68440626480706
10-11	24.275991555310483	26.99849206062907	28.520946815290454	20.204569568769994
12-13	24.81006714627954	24.4129299355307	28.529459973845807	22.24754294434396
14-15	22.14151030636665	25.893040937162613	25.975652770910557	25.98979598556018
16-17	22.19627144857567	28.999620937858143	26.587966895621346	22.21614071794484
18-19	22.32011274468754	28.526932538566314	27.618304263212178	21.53465045353397
20-21	23.231289240554602	27.621962857616566	27.372449061807647	21.774298840021185
22-23	22.979200287856518	27.47026372631554	27.17573403949928	22.374801946328667
24-25	22.665544031469945	27.62945261561876	27.28284043349158	22.422162919419716
26-27	22.6255166448187	27.70792700566783	27.201918886440268	22.4646374630732
28-29	22.590442021004403	27.739443504964317	27.24565073756461	22.424463736466667
30-31	22.836454676859137	27.536012400110383	27.154939736408355	22.472593186622124
32-33	22.580480210574084	27.621346878481255	27.188274007679876	22.60989890326479
34-35	22.71891337209689	27.607133661439338	27.210835994101373	22.4631169723624
36-37	22.731384784178857	27.351857722041533	27.255262544635556	22.661494949144053
38-39	22.48957974207418	27.44034740987238	27.513521147376967	22.556551700676472
40-41	22.624672225169384	27.219205743708585	27.56968050824585	22.586441522876182
42-43	22.34740411808336	27.263624665487963	27.534213971287457	22.85475724514122
44-45	22.288526146527047	27.24991300255621	27.649517368543357	22.812043482373383
46-47	22.237786097414926	27.10138091043259	27.770290364338734	22.890542627813755
48-49	22.10761574921659	27.180811167966233	27.7993139582435	22.912259124573676
50-51	21.897677512935143	27.20330861356206	27.910022939131558	22.98899093437124
52-53	22.018624430171112	27.17714549783607	27.863142670541997	22.94108740145083
54-55	22.001194949973442	26.995894293340328	27.964606789997486	23.038303966688744
56-57	21.96691571947996	27.112297393193096	27.84214871061938	23.078638176707567
58-59	21.89006326019384	27.075349655248544	27.846788203782687	23.187798880774928
60-61	21.888969542545627	26.94512177056174	27.833143221142453	23.332765465750178
62-63	21.91022094854431	26.920139227036387	27.832261587209594	23.33737823720971
64-65	22.01752330379644	26.970489644148294	27.68994448798548	23.32204256406979
66-67	21.936426100115384	27.151127044812668	27.35129171854689	23.56115513652506
68-69	21.969470396391387	27.245786465591582	27.252187089317122	23.532556048699906
70-71	22.026490083559338	27.189153306264174	27.10132211767008	23.6830344925064
72-73	22.188448311175232	27.184248136914093	26.6955126083405	23.931790943570178
74-75	22.244376225870738	27.067925522088277	26.608700095514738	24.07899815652625
76-77	22.478590934033146	26.90134348404157	26.667427884968998	23.95263769695629
78-79	22.57509899625399	26.689989076275424	26.839127544789847	23.895784382680745
80-81	22.759893301764507	26.61159643410912	26.774283410474702	23.85422685365167
82-83	22.733876936803636	26.69911267284179	26.775695313839403	23.791315076515176
84-85	22.77759958083601	26.54609544377476	26.907422863581935	23.76888211180729
86-87	22.516084062166268	26.802376318667147	27.06440232857061	23.617137290595977
88-89	22.584055402335338	26.916946165830286	26.88946794448775	23.609530487346625
90-91	22.54235636335902	27.152298588792117	27.081378030410562	23.2239670174383
92-93	22.312606160800627	27.15075751739075	27.38995458711864	23.146681734689984
94-95	22.140928400066485	27.242103738046943	27.614310646366018	23.00265721552055
96-97	22.17568434921096	27.179677673453583	27.877040378960523	22.767597598374934
98-99	23.591478407677858	29.224696652512105	27.930044051115168	19.25378088869487
100	22.920072752683783	30.39802058056782	21.537592243450028	25.144314423298376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2922.0
1	2178.5
2	1511.5
3	1532.0
4	1449.0
5	1465.5
6	1650.0
7	1914.0
8	2143.0
9	2434.0
10	2726.0
11	2897.5
12	3148.5
13	3480.5
14	3856.5
15	4438.5
16	5223.5
17	6210.5
18	7373.5
19	8741.0
20	10479.0
21	12980.0
22	16732.5
23	21625.5
24	27771.5
25	36001.5
26	46437.0
27	58091.0
28	70572.0
29	84407.5
30	97272.0
31	110289.0
32	124901.0
33	139107.5
34	153491.0
35	167213.0
36	182844.5
37	198977.0
38	211119.5
39	222387.5
40	235172.5
41	246661.0
42	255624.5
43	264814.5
44	274056.5
45	283880.5
46	290914.5
47	296265.0
48	300417.0
49	301649.5
50	300510.0
51	293990.0
52	280848.5
53	262683.5
54	245263.0
55	228011.5
56	209131.0
57	187584.5
58	165554.0
59	145020.0
60	123056.0
61	103165.0
62	84564.0
63	67979.0
64	55289.0
65	44314.0
66	35599.0
67	27126.5
68	19606.5
69	15305.5
70	11986.0
71	8594.5
72	6557.5
73	5062.5
74	3808.0
75	2847.0
76	2152.5
77	1698.5
78	1288.0
79	999.0
80	793.5
81	610.5
82	490.5
83	329.0
84	209.5
85	160.5
86	129.5
87	102.0
88	68.0
89	43.5
90	28.0
91	21.5
92	19.0
93	11.5
94	4.5
95	3.5
96	5.0
97	4.0
98	2.0
99	4.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012024569631348167
3	0.02201641439644462
4	0.01878123256705809
5	0.010049104620483825
6	0.012983672209086652
7	0.014501058376852017
8	0.0019038901916301266
9	1.002047469279014E-4
10-11	7.157481923421529E-5
12-13	0.002891622697062297
14-15	2.1472445770264584E-4
16-17	5.010237346395071E-4
18-19	2.8629927693686115E-5
20-21	2.148572653053215E-5
22-23	0.0
24-25	2.8697210494828155E-5
26-27	0.002549582741034141
28-29	0.003645765881570999
30-31	0.002578008892834475
32-33	0.0040891680966134115
34-35	0.0057864563546235195
36-37	0.0010928083009429054
38-39	0.0014071674582225402
40-41	5.744024596640414E-4
42-43	7.285259508593218E-5
44-45	3.6492203039406396E-5
46-47	0.004072798531745161
48-49	7.619046502703808E-4
50-51	3.0827138362986474E-4
52-53	0.004243040788049597
54-55	2.0627947125856559E-4
56-57	1.1070641765103124E-4
58-59	5.175477905478177E-4
60-61	2.2220900490141215E-5
62-63	0.0
64-65	7.474854961651752E-6
66-67	0.001667382408798709
68-69	0.0
70-71	2.617453410291596E-4
72-73	0.0014779540977016335
74-75	1.415031152696955E-4
76-77	7.951444029944502E-6
78-79	4.025545467449158E-5
80-81	0.0028892999022290295
82-83	1.0761790742111565E-4
84-85	2.519008014643833E-4
86-87	0.012770975069061732
88-89	6.847837920864651E-4
90-91	8.827474773725339E-4
92-93	9.000483685993285E-5
94-95	2.574101960178643E-4
96-97	1.889436237573178E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2908.0
20-21	5493.0
22-23	6282.0
24-25	7039.0
26-27	8452.0
28-29	9387.0
30-31	10612.0
32-33	11072.0
34-35	12455.0
36-37	14717.0
38-39	16325.0
40-41	14885.0
42-43	12076.0
44-45	12742.0
46-47	13077.0
48-49	12773.0
50-51	12897.0
52-53	12432.0
54-55	12424.0
56-57	11992.0
58-59	11993.0
60-61	13348.0
62-63	36464.0
64-65	60523.0
66-67	65016.0
68-69	66475.0
70-71	67632.0
72-73	66522.0
74-75	70655.0
76-77	76422.0
78-79	83014.0
80-81	86429.0
82-83	84816.0
84-85	88146.0
86-87	94817.0
88-89	102373.0
90-91	107930.0
92-93	113716.0
94-95	129783.0
96-97	277866.0
98-99	493913.0
100-101	4567804.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.63046328076793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85202429700055	76.94070467266974
2	7.258703422163556	12.43132273715122
3	1.6517638611251724	4.243239139757356
4	0.5767572727267488	1.9755196985657488
5	0.2519168952953222	1.0785880226195574
6	0.13980161616086192	0.718276629555282
7	0.07392847825914674	0.44313708900810417
8	0.04928292721690313	0.33760959115326256
9	0.035007163752929026	0.26979116852781165
>10	0.10883063142304167	1.3845476989570238
>50	0.001662789316944664	0.09636214293079623
>100	2.691619216793637E-4	0.043318369933944306
>500	2.5741820281774637E-5	0.015518159334787156
>1k	2.5741816818246318E-5	0.02206487983534931
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4314963846843056E-5
2	0.0	1.4314963846843056E-5	0.0	1.4314963846843056E-5	1.4314963846843056E-5
3	0.0	1.4314963846843056E-5	0.0	1.4314963846843056E-5	1.4314963846843056E-5
4	0.0	8.588978308105833E-5	0.0	5.725985538737222E-5	1.4314963846843056E-5
5	0.0	1.1451971077474445E-4	0.0	5.725985538737222E-5	1.4314963846843056E-5
6	0.0	1.1451971077474445E-4	0.0	7.157481923421529E-5	1.4314963846843056E-5
7	0.0	1.2883467462158752E-4	0.0	8.588978308105833E-5	1.4314963846843056E-5
8	0.0	1.4314963846843057E-4	0.0	8.588978308105833E-5	1.4314963846843056E-5
9	0.0	2.4335438539633197E-4	0.0	1.002047469279014E-4	1.4314963846843056E-5
10-11	0.0	2.4335438539633197E-4	0.0	1.2883467462158752E-4	2.1472445770264584E-5
12-13	0.0	2.4335438539633197E-4	0.0	2.2188193962606738E-4	3.5787409617107636E-5
14-15	0.0	3.721890600179195E-4	0.0	2.7198431309001807E-4	5.725985538737222E-5
16-17	4.294489154052917E-5	3.721890600179195E-4	0.0	3.435591323242334E-4	7.157481923421529E-5
18-19	4.294489154052917E-5	4.151339515584486E-4	0.0	3.7218906001791944E-4	9.304726500447986E-5
20-21	4.294489154052917E-5	4.7239380694582086E-4	0.0	4.938662527160854E-4	1.002047469279014E-4
22-23	4.294489154052917E-5	5.296536623331931E-4	0.0	8.517403488871619E-4	1.1451971077474446E-4
24-25	4.294489154052917E-5	5.439686261800361E-4	0.0	0.0019539925650940773	1.4314963846843057E-4
26-27	4.294489154052917E-5	5.582835900268792E-4	0.0	0.005038867274088756	1.4314963846843057E-4
28-29	4.294489154052917E-5	5.725985538737222E-4	0.0	0.021808847420665398	1.503071203918521E-4
30-31	4.294489154052917E-5	6.871182646484667E-4	0.0	0.07704313542370933	1.5746460231527362E-4
32-33	4.294489154052917E-5	6.942757465718882E-4	0.0	0.2067009204664903	1.5746460231527362E-4
34-35	4.294489154052917E-5	7.944804934997896E-4	0.0	0.39058378856111275	1.8609453000895975E-4
36-37	4.294489154052917E-5	8.875277585042695E-4	0.0	0.6729536079220155	2.075669757792243E-4
38-39	4.294489154052917E-5	9.734175415853279E-4	0.0	1.055671323849288	2.1472445770264584E-4
40-41	4.294489154052917E-5	0.0010593073246663862	0.0	1.4459115532780766	2.1472445770264584E-4
42-43	4.294489154052917E-5	0.0010950947342834938	0.0	1.8820169268721503	2.1472445770264584E-4
44-45	4.294489154052917E-5	0.0012096144450582382	0.0	2.4029814061503094	2.1472445770264584E-4
46-47	4.294489154052917E-5	0.0013026617100627181	0.0	2.9960217283973236	2.2188193962606738E-4
48-49	4.294489154052917E-5	0.001316976673909561	0.0	3.594866768484233	2.290394215494889E-4
50-51	5.725985538737222E-5	0.0015030712039185209	0.0	4.175488859594111	2.290394215494889E-4
52-53	5.725985538737222E-5	0.0015245436496887856	0.0	4.742969813892587	2.3619690347291043E-4
54-55	5.725985538737222E-5	0.0015317011316122071	0.0	5.33052750498626	2.4335438539633197E-4
56-57	5.725985538737222E-5	0.00154601609545905	0.0	5.9694830737720235	2.4335438539633197E-4
58-59	5.725985538737222E-5	0.0015531735773824716	0.0	6.638371231961536	2.4335438539633197E-4
60-61	7.157481923421529E-5	0.0016032759508464224	0.0	7.291577347256831	2.5766934924317504E-4
62-63	7.157481923421529E-5	0.00163906336046353	0.0	7.90157374418043	2.7198431309001807E-4
64-65	7.157481923421529E-5	0.0018466303362427543	0.0	8.059568000158038	2.7198431309001807E-4
66-67	7.157481923421529E-5	0.0018537878181661758	0.0	8.062989276517431	2.7914179501343963E-4
68-69	7.157481923421529E-5	0.001911047673553548	0.0	8.065952474033729	2.9345675886028265E-4
70-71	7.873230115763681E-5	0.0019396776012472342	0.0	8.068164135948066	3.3640165040081183E-4
72-73	8.588978308105833E-5	0.0019468350831706558	0.0	8.070225490742011	3.721890600179195E-4
74-75	8.588978308105833E-5	0.0019754650108643416	0.0	8.072064963596333	3.721890600179195E-4
76-77	8.588978308105833E-5	0.002032724866251714	0.0	8.073868649041033	3.721890600179195E-4
78-79	8.588978308105833E-5	0.0020971422035625078	0.0	8.075164153269172	3.721890600179195E-4
80-81	8.588978308105833E-5	0.0021042996854859293	0.0	8.075858429015746	3.936615057881841E-4
82-83	8.588978308105833E-5	0.0021042996854859293	0.0	8.076302192894996	4.294489154052917E-4
84-85	8.588978308105833E-5	0.0021472445770264583	0.0	8.076466814979234	4.294489154052917E-4
86-87	8.588978308105833E-5	0.0021615595408733014	0.0	8.076524074834623	4.294489154052917E-4
88	8.588978308105833E-5	0.002218819396260674	0.0	8.076531232316546	4.294489154052917E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5555	0.0	39.10607	1
GTATCAA	9035	0.0	32.4151	1
TCAACGC	9690	0.0	27.907938	4
ATCAACG	9975	0.0	27.24379	3
CAACGCA	10060	0.0	26.83549	5
AACGCAG	10405	0.0	25.94254	6
TATCAAC	10870	0.0	25.769808	2
GTACATG	22615	0.0	21.233782	1
ACGCAGA	12795	0.0	21.095482	7
TACATGG	22570	0.0	20.925413	2
ACATGGG	22670	0.0	19.979181	3
CGCAGAG	13855	0.0	19.41641	8
CATGGGG	17375	0.0	17.841885	4
GTGGTAT	4075	0.0	17.266077	1
TGGTATC	3910	0.0	16.307693	2
GCAGAGT	16715	0.0	16.22533	9
AGAGTAC	14520	0.0	15.242197	10-11
GTGTAGC	3770	0.0	14.813705	1
GTATAGG	2220	0.0	14.658179	1
CAGAGTA	16350	0.0	14.557789	10-11
>>END_MODULE