FastQCFastQC Report
Fri 27 May 2016
SRR2140248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822706
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52550.038.9554671
GTATCAA90800.032.1461031
TCAACGC95050.028.836034
ATCAACG97900.028.0868193
CAACGCA99750.027.4330565
AACGCAG104950.026.2771516
TATCAAC108750.026.002642
ACGCAGA127750.021.4849197
CGCAGAG131850.020.6513358
GTACATG217850.020.3267211
TACATGG215600.019.935362
TGTAGAT97150.019.8696194
ACATGGG221300.019.1669673
GTGTAGA98850.018.6760194
GGTGTAG106250.018.65556594
CATGGGG158400.017.1665424
GCAGAGT164950.016.2438459
AGAGTAC137750.015.81601810-11
TTAGGTG158450.015.28956894
AGGTGTA125400.014.928594
Produced by FastQC (version 0.11.5)