FastQCFastQC Report
Fri 27 May 2016
SRR2140247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7922510
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.037.742461
GTATCAA99150.031.3436831
TCAACGC99100.029.6869454
ATCAACG101700.028.8879283
CAACGCA104050.028.1884925
AACGCAG109350.026.9390836
TATCAAC116150.026.2315142
ACGCAGA132050.022.3072877
CGCAGAG135400.021.6570518
GTACATG219000.019.7554451
TACATGG220450.019.0134412
ACATGGG224850.018.0989063
ACCGAAC9250.016.4620698
AGGACCG15800.016.2498365
GCAGAGT176900.016.231989
CATGGGG169250.015.8138964
AGAGTAC149500.015.23454510-11
CAGAGTA171050.014.48520510-11
CCGAACC10400.013.3866539
GTGTAGA103150.013.15422594
Produced by FastQC (version 0.11.5)