FastQCFastQC Report
Fri 27 May 2016
SRR2140246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7657917
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.037.5380361
GTATCAA102850.030.713981
TCAACGC106650.027.4558584
ATCAACG109600.026.7575513
CAACGCA111600.026.2749355
AACGCAG116300.025.2471546
TATCAAC123150.024.819172
ACGCAGA138500.021.10767
CGCAGAG141450.020.6353328
GTACATG233450.019.6024151
TACATGG232950.019.1497122
ACATGGG238850.018.0150473
CATGGGG170200.016.4017434
GCAGAGT183450.015.7479349
AGAGTAC155750.015.54867410-11
CAGAGTA175150.014.49647210-11
GTCTAGG21950.013.6135741
GTGTAGC43600.013.315611
GTGGTAT45300.013.2870811
GTATACG8400.013.2130391
Produced by FastQC (version 0.11.5)