##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7657917
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444177835826636	31.0	31.0	34.0	30.0	34.0
2	31.547145261563948	31.0	31.0	34.0	30.0	34.0
3	31.657844816025037	31.0	31.0	34.0	30.0	34.0
4	35.301565948024766	37.0	35.0	37.0	33.0	37.0
5	35.4211466381785	37.0	35.0	37.0	33.0	37.0
6	35.56126581157774	37.0	35.0	37.0	33.0	37.0
7	35.52975828805666	37.0	35.0	37.0	33.0	37.0
8	35.477908157009274	37.0	35.0	37.0	33.0	37.0
9	37.14182198631821	39.0	37.0	39.0	33.0	39.0
10-11	37.144375748653324	39.0	37.0	39.0	33.5	39.0
12-13	37.094223533631926	39.0	37.0	39.0	33.0	39.0
14-15	38.477580718098665	40.0	38.0	41.0	33.0	41.0
16-17	38.478934872237446	40.0	38.0	41.0	33.0	41.0
18-19	38.4969976822679	40.0	38.0	41.0	33.5	41.0
20-21	38.55435118249288	40.0	38.0	41.0	34.0	41.0
22-23	38.53760445530607	40.0	38.0	41.0	34.0	41.0
24-25	38.570292135151476	40.0	38.0	41.0	34.0	41.0
26-27	38.55728531036308	40.0	38.0	41.0	33.5	41.0
28-29	38.49316504552629	40.0	38.0	41.0	33.0	41.0
30-31	38.42464055923307	40.0	38.0	41.0	33.0	41.0
32-33	38.387744737516215	40.0	38.0	41.0	33.0	41.0
34-35	38.31760642588722	40.0	38.0	41.0	33.0	41.0
36-37	38.23565352526756	40.0	38.0	41.0	33.0	41.0
38-39	38.1504922767363	40.0	38.0	41.0	33.0	41.0
40-41	38.10489517343882	40.0	38.0	41.0	33.0	41.0
42-43	37.95425665639538	40.0	37.5	41.0	32.0	41.0
44-45	37.77920998785088	40.0	37.0	41.0	32.0	41.0
46-47	37.63464851615371	40.0	37.0	41.0	32.0	41.0
48-49	37.49854568044009	40.0	36.5	41.0	31.0	41.0
50-51	37.366682891314554	39.0	36.0	41.0	31.0	41.0
52-53	37.21554176392114	39.0	36.0	41.0	31.0	41.0
54-55	37.035063215972905	39.0	35.5	40.0	30.5	41.0
56-57	37.00362979293814	39.0	35.5	40.0	30.5	41.0
58-59	37.057971676704696	39.0	35.0	41.0	31.0	41.0
60-61	37.348728014604404	39.0	36.0	41.0	31.5	41.0
62-63	37.29652282778642	39.0	35.0	41.0	31.5	41.0
64-65	37.07377277224765	39.0	35.0	41.0	31.0	41.0
66-67	36.846324334032786	38.0	35.0	40.5	31.0	41.0
68-69	36.544263011331	37.5	35.0	40.0	31.0	41.0
70-71	36.2783718838919	37.0	35.0	40.0	31.0	41.0
72-73	35.9621321143115	36.5	35.0	39.0	31.0	41.0
74-75	35.64075046691881	36.0	34.0	39.0	31.0	41.0
76-77	35.32864329351263	36.0	34.0	38.5	30.0	40.0
78-79	35.01926354213451	35.0	34.0	37.0	30.0	39.0
80-81	34.719364753459594	35.0	34.0	37.0	30.0	39.0
82-83	34.47106036310476	35.0	34.0	36.5	30.0	39.0
84-85	34.2424360736011	35.0	34.0	36.0	30.0	37.5
86-87	34.04997923103478	35.0	34.0	36.0	30.0	37.0
88-89	33.90733764956293	35.0	34.0	35.5	30.0	37.0
90-91	33.78622288333241	35.0	34.0	35.0	30.0	36.0
92-93	33.721314218578044	35.0	34.0	35.0	30.0	36.0
94-95	33.6972903274156	35.0	34.0	35.0	30.0	36.0
96-97	33.69318839004151	35.0	34.0	35.0	30.0	36.0
98-99	33.722698056301	35.0	34.0	35.0	30.0	36.0
100	33.03583567024151	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	4.0
16	40.0
17	258.0
18	1072.0
19	3666.0
20	9058.0
21	17383.0
22	27068.0
23	38006.0
24	50608.0
25	63412.0
26	71324.0
27	74512.0
28	85548.0
29	104466.0
30	122705.0
31	147094.0
32	187428.0
33	246365.0
34	338622.0
35	482923.0
36	771592.0
37	1419906.0
38	2057761.0
39	1277390.0
40	59704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.20032901897474	20.391106354378092	14.452938050908623	23.955626575738545
2	14.129657432772696	20.85898303880576	42.103913173396194	22.907446355025357
3	17.90150232471692	25.981044646032675	30.33991662444599	25.77753640480442
4	12.344422104540822	18.083828362320205	39.308196718742124	30.26355281439685
5	13.403927103891478	38.05431894711658	35.563690587508866	12.97806336148308
6	29.924707646255232	37.761564200281576	19.280667431126762	13.033060722336426
7	26.677753256038045	32.77376226671079	23.141398550654603	17.407085926596558
8	24.1797529667315	36.96105933933109	21.411826496385235	17.44736119755218
9	25.22541098402813	17.80485279190734	21.515295118074786	35.45444110598975
10-11	23.77463677114117	27.171769906500636	29.21284167109237	19.840751651265823
12-13	24.423582623948295	24.73402876507521	29.059446002573363	21.782942608403136
14-15	21.823552505951863	26.357864367097577	26.402210737057953	25.416372389892604
16-17	21.77013689575079	29.32695000999625	27.08375474071057	21.819158353542388
18-19	21.819292730508714	28.9682551341811	28.145576952370394	21.066875182939796
20-21	22.744290020447057	28.036467784632514	27.881699043842197	21.337543151078236
22-23	22.459877074970986	27.931168990411077	27.662857101026344	21.946096833591593
24-25	22.15406619995954	28.114082297699216	27.702861312400078	22.028990189941165
26-27	22.14085301110707	28.23823459537006	27.604188454392403	22.01672393913047
28-29	22.05048759477951	28.27325494248819	27.69133042711888	21.984927035613417
30-31	22.33409483907062	28.071749449974405	27.626635200811116	21.96752051014386
32-33	22.071984307226124	28.131615769551736	27.64146317454095	22.154936748681195
34-35	22.230405919358855	28.075946759562353	27.735073844705525	21.958573476373257
36-37	22.207968157813436	27.89741883463961	27.762135525914854	22.132477481632097
38-39	22.048886125274635	27.92981967416988	27.953883016208597	22.067411184346888
40-41	22.186836447331853	27.748621835497424	27.975413795740423	22.0891279214303
42-43	21.973558619442	27.80429813665759	27.91583273113477	22.30631051276564
44-45	22.025776526166087	27.873025024282455	27.940683281879057	22.1605151676724
46-47	21.89016978598833	27.84737123865032	27.891960665026787	22.370498310334565
48-49	21.79386424080324	27.908478417715305	27.97583476875749	22.32182257272396
50-51	21.686219173092	28.011834978024414	27.98874858887958	22.313197260004
52-53	21.855892189387202	27.95269478600287	27.848051941692702	22.34336108291723
54-55	21.904460267590782	27.852522789954463	27.8393111215311	22.403705820923644
56-57	21.914173976419466	27.90056959085464	27.770022173960466	22.415234258765423
58-59	21.949148535087946	27.84718306230949	27.809029642652224	22.394638759950343
60-61	22.056274995205094	27.771154686690203	27.783903118188004	22.3886671999167
62-63	22.087221996236988	27.91527182670015	27.58441985916728	22.413086317895587
64-65	22.179392845709565	27.92164139232906	27.456887524500484	22.44207823746089
66-67	22.174230514772375	27.92981557211864	27.365220085540866	22.530733827568124
68-69	22.191006326292108	27.845209511212097	27.42810526736768	22.535678895128118
70-71	22.213147150699985	27.756399491453255	27.50418504381128	22.526268314035473
72-73	22.354033511767398	27.713785867313558	27.46788572972153	22.464294891197518
74-75	22.284884977527955	27.874818885617508	27.533828314211302	22.30646782264324
76-77	22.376817780446974	27.79944431837799	27.510117931504404	22.313619969670633
78-79	22.331601646782175	27.755805621080647	27.54154498812843	22.371047744008745
80-81	22.330984794664747	27.87287655233513	27.49957019468685	22.296568458313274
82-83	22.325011836785897	27.871936093496448	27.692025041378166	22.111027028339493
84-85	22.42398546673844	27.930130546707353	27.615685645775894	22.030198340778306
86-87	22.168471446863744	28.00469322072272	27.742213093735575	22.08462223867796
88-89	22.234954371245593	27.958868047613834	27.723915877404643	22.082261703735927
90-91	22.230864737057814	28.038760804946655	27.6958534814886	22.034520976506926
92-93	22.208471763626907	27.995066956250337	27.736892802649688	22.05956847747307
94-95	22.064618992743767	28.095761712376827	27.801481602072435	22.038137692806966
96-97	22.15968187491454	28.233783170457087	27.826407698775448	21.78012725585293
98-99	23.192711715799224	30.162348477884173	27.975428285050874	18.669511521265733
100	22.143287274214885	31.191209739756992	21.753232077842735	24.912270908185388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2448.0
1	1808.5
2	1300.0
3	1422.0
4	1379.5
5	1472.5
6	1754.0
7	2075.5
8	2350.5
9	2657.0
10	3049.5
11	3367.5
12	3695.5
13	4150.5
14	4727.0
15	5578.5
16	6700.0
17	8002.5
18	9678.5
19	11981.5
20	15070.5
21	19185.0
22	24696.5
23	31764.0
24	40528.5
25	51994.0
26	66462.0
27	82605.5
28	99947.5
29	119544.5
30	138303.5
31	155597.0
32	174231.0
33	191703.5
34	208196.0
35	223954.5
36	240545.0
37	257208.5
38	270092.5
39	280094.5
40	290458.5
41	299177.5
42	304874.5
43	310842.5
44	315314.5
45	320923.5
46	325417.5
47	325910.5
48	324068.0
49	319322.5
50	313650.0
51	303821.5
52	288943.0
53	272221.0
54	254843.5
55	236125.0
56	216045.0
57	194712.5
58	172936.5
59	150069.0
60	125332.5
61	102152.5
62	81728.0
63	63910.5
64	49169.0
65	37699.5
66	28830.5
67	22556.0
68	17792.5
69	13816.0
70	10763.0
71	8229.5
72	6313.5
73	4870.0
74	3787.5
75	2979.0
76	2248.5
77	1692.0
78	1279.0
79	941.5
80	699.0
81	529.0
82	421.5
83	293.0
84	191.0
85	135.5
86	109.0
87	88.5
88	63.5
89	41.5
90	29.0
91	26.5
92	23.5
93	13.0
94	5.0
95	4.5
96	4.0
97	4.0
98	3.5
99	2.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011870068583924323
3	0.022408182277243276
4	0.019104411813290742
5	0.010094128729783831
6	0.014325044264647946
7	0.015918166780862213
8	0.0021285161487125024
9	2.089341004871168E-4
10-11	5.22335251217792E-5
12-13	0.0029773109319414146
14-15	2.4158005368822878E-4
16-17	6.006855389004608E-4
18-19	1.95875719206672E-5
20-21	8.491567155297569E-5
22-23	6.5360617731041445E-6
24-25	1.9621814382083556E-5
26-27	0.0027429278286590524
28-29	0.003925745603296001
30-31	0.0027896811466652876
32-33	0.004293747002431984
34-35	0.006338906541257353
36-37	0.0012024451126750532
38-39	0.001386209694129183
40-41	6.461457439678798E-4
42-43	6.685519385632858E-5
44-45	7.391402159740833E-5
46-47	0.0041038166915002795
48-49	9.258519233280237E-4
50-51	3.015132263573543E-4
52-53	0.004434348199089131
54-55	2.707586302751331E-4
56-57	2.1689057003179614E-4
58-59	6.563530778689529E-4
60-61	2.1366081744064824E-5
62-63	0.0
64-65	2.1762889833204858E-5
66-67	0.001839349729047663
68-69	0.0
70-71	4.41730836181499E-4
72-73	0.001786680899548409
74-75	1.6083712506855682E-4
76-77	1.5510565952632593E-5
78-79	3.144993806720946E-5
80-81	0.003310687697644067
82-83	6.47481886289555E-5
84-85	3.86032109822357E-4
86-87	0.014799633802863876
88-89	8.550106757818141E-4
90-91	0.0010231058570660935
92-93	2.6206132898987062E-5
94-95	2.8436214372089285E-4
96-97	8.182597016279641E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2163.0
20-21	4558.0
22-23	5178.0
24-25	6264.0
26-27	8235.0
28-29	10491.0
30-31	13545.0
32-33	14907.0
34-35	18900.0
36-37	27036.0
38-39	32312.0
40-41	28560.0
42-43	33775.0
44-45	43865.0
46-47	50776.0
48-49	48067.0
50-51	47449.0
52-53	46947.0
54-55	53254.0
56-57	60995.0
58-59	64027.0
60-61	63560.0
62-63	63869.0
64-65	68267.0
66-67	71543.0
68-69	72709.0
70-71	73804.0
72-73	75074.0
74-75	79582.0
76-77	86084.0
78-79	91783.0
80-81	90353.0
82-83	89590.0
84-85	91125.0
86-87	90451.0
88-89	90393.0
90-91	91727.0
92-93	94540.0
94-95	109987.0
96-97	262851.0
98-99	478462.0
100-101	4800859.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.93722764602157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.95493881448608	77.30478054784432
2	7.095121918691273	12.194702150056987
3	1.7069205451595764	4.400640583891493
4	0.6186397685189742	2.126567464723887
5	0.2584624899083176	1.1105774916604323
6	0.13488805983599628	0.6955143542913714
7	0.06872443959626792	0.41341914673008157
8	0.04182683281264074	0.28755856425055937
9	0.03114799612287092	0.24090951901757
>10	0.08814334746734584	1.1325050878539928
>50	9.91806212316342E-4	0.054898383533638365
>100	1.7095517881476237E-4	0.023936650811068008
>500	2.302600949000288E-5	0.013990055334605343
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.3058381280444799E-5	0.0
3	0.0	0.0	0.0	2.6116762560889598E-5	0.0
4	0.0	2.6116762560889598E-5	0.0	1.0446705024355839E-4	0.0
5	0.0	2.6116762560889598E-5	0.0	1.436421940848928E-4	0.0
6	0.0	2.6116762560889598E-5	0.0	1.567005753653376E-4	0.0
7	1.3058381280444799E-5	2.6116762560889598E-5	0.0	1.697589566457824E-4	0.0
8	2.6116762560889598E-5	3.91751438413344E-5	0.0	1.828173379262272E-4	0.0
9	3.91751438413344E-5	9.14086689631136E-5	0.0	2.0893410048711679E-4	0.0
10-11	3.91751438413344E-5	9.14086689631136E-5	0.0	3.7216386649267677E-4	6.5291906402223995E-6
12-13	3.91751438413344E-5	9.7937859603336E-5	0.0	4.048098196937888E-4	1.3058381280444799E-5
14-15	3.91751438413344E-5	1.371130034446704E-4	0.0	4.24397391614456E-4	1.9587571920667198E-5
16-17	5.2233525121779196E-5	1.436421940848928E-4	0.0	4.7663091673623523E-4	2.6116762560889598E-5
18-19	7.83502876826688E-5	1.501713847251152E-4	0.0	5.22335251217792E-4	2.6116762560889598E-5
20-21	1.044670502435584E-4	1.6322976600556E-4	0.0	6.463898733820176E-4	2.6116762560889598E-5
22-23	1.240546221642256E-4	1.95875719206672E-4	0.0	0.0010707872649964737	3.9175143841334396E-5
24-25	1.30583812804448E-4	2.481092443284512E-4	0.0	0.0022264540083158386	5.2233525121779196E-5
26-27	1.436421940848928E-4	2.742260068893408E-4	0.0	0.00459655021071657	5.2233525121779196E-5
28-29	1.436421940848928E-4	2.872843881697856E-4	0.0	0.01892812366600474	5.2233525121779196E-5
30-31	1.501713847251152E-4	3.786930571328992E-4	0.0	0.06787746589575207	5.2233525121779196E-5
32-33	1.567005753653376E-4	3.786930571328992E-4	0.0	0.1889351895561156	5.2233525121779196E-5
34-35	1.567005753653376E-4	4.57043344815568E-4	0.0	0.3512182229188433	5.2233525121779196E-5
36-37	1.697589566457824E-4	5.484520137786816E-4	0.0	0.5862952027294106	5.2233525121779196E-5
38-39	1.697589566457824E-4	5.810979669797936E-4	0.0	0.8536198551120363	5.2233525121779196E-5
40-41	1.697589566457824E-4	6.268023014613504E-4	0.0	1.1019119167784137	5.2233525121779196E-5
42-43	1.697589566457824E-4	6.333314921015728E-4	0.0	1.4249697404659778	5.2233525121779196E-5
44-45	1.762881472860048E-4	6.85565017223352E-4	0.0	1.6745154067352779	5.2233525121779196E-5
46-47	1.828173379262272E-4	7.312693517049088E-4	0.0	1.9026518568952888	5.2233525121779196E-5
48-49	2.0240490984689438E-4	7.443277329853535E-4	0.0	2.062564794055616	5.2233525121779196E-5
50-51	2.350508630480064E-4	8.292072113082448E-4	0.0	2.246296218671474	5.2233525121779196E-5
52-53	2.5463843496867356E-4	8.357364019484671E-4	0.0	2.426867515017465	5.2233525121779196E-5
54-55	2.61167625608896E-4	8.683823551495792E-4	0.0	2.5960453736962674	5.2233525121779196E-5
56-57	2.676968162491184E-4	8.879699270702464E-4	0.0	2.728901344843513	5.2233525121779196E-5
58-59	2.8075519752956317E-4	8.879699270702464E-4	0.0	2.7890417198306015	5.2233525121779196E-5
60-61	2.872843881697856E-4	9.206158802713584E-4	0.0	2.8112997307231193	5.2233525121779196E-5
62-63	2.872843881697856E-4	9.402034521920255E-4	0.0	2.8248739180641422	5.2233525121779196E-5
64-65	2.9381357881000803E-4	0.0010838456462769184	0.0	2.829352942843334	5.2233525121779196E-5
66-67	3.003427694502304E-4	0.0010838456462769184	0.0	2.8329897020299386	5.2233525121779196E-5
68-69	3.068719600904528E-4	0.0010969040275573632	0.0	2.836430585497336	5.2233525121779196E-5
70-71	3.134011507306752E-4	0.0011295499807584752	0.0	2.8393099585696735	5.2233525121779196E-5
72-73	3.2645953201111997E-4	0.0011360791713986977	0.0	2.8420065143040856	7.83502876826688E-5
74-75	3.2645953201111997E-4	0.001155666743319365	0.0	2.8444092564596875	7.83502876826688E-5
76-77	3.2645953201111997E-4	0.001207900268441144	0.0	2.8466552980399236	7.83502876826688E-5
78-79	3.2645953201111997E-4	0.0012274878403618112	0.0	2.8480264280743706	7.83502876826688E-5
80-81	3.329887226513424E-4	0.0012274878403618112	0.0	2.848803401760557	8.48794783228912E-5
82-83	3.395179132915648E-4	0.0012536046029227007	0.0	2.8493126786304943	9.14086689631136E-5
84-85	3.395179132915648E-4	0.0012862505561238129	0.0	2.8494889667777805	9.14086689631136E-5
86-87	3.395179132915648E-4	0.0013188965093249249	0.0	2.849534671112262	9.14086689631136E-5
88	3.395179132915648E-4	0.0013450132718858144	0.0	2.849534671112262	9.14086689631136E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6175	0.0	37.538036	1
GTATCAA	10285	0.0	30.71398	1
TCAACGC	10665	0.0	27.455858	4
ATCAACG	10960	0.0	26.757551	3
CAACGCA	11160	0.0	26.274935	5
AACGCAG	11630	0.0	25.247154	6
TATCAAC	12315	0.0	24.81917	2
ACGCAGA	13850	0.0	21.1076	7
CGCAGAG	14145	0.0	20.635332	8
GTACATG	23345	0.0	19.602415	1
TACATGG	23295	0.0	19.149712	2
ACATGGG	23885	0.0	18.015047	3
CATGGGG	17020	0.0	16.401743	4
GCAGAGT	18345	0.0	15.747934	9
AGAGTAC	15575	0.0	15.548674	10-11
CAGAGTA	17515	0.0	14.496472	10-11
GTCTAGG	2195	0.0	13.613574	1
GTGTAGC	4360	0.0	13.31561	1
GTGGTAT	4530	0.0	13.287081	1
GTATACG	840	0.0	13.213039	1
>>END_MODULE