FastQCFastQC Report
Fri 27 May 2016
SRR2140245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7328972
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59800.039.0416371
GTATCAA98600.029.218251
TCAACGC102550.026.4163044
ATCAACG105000.025.8848863
CAACGCA105300.025.7241365
AACGCAG111750.024.3162256
TATCAAC115350.024.1734962
GTACATG226400.021.4416661
TACATGG227700.020.3906442
ACGCAGA133800.020.2097937
CGCAGAG139000.019.3241088
ACATGGG231500.019.294673
CATGGGG173650.016.6149524
TCGGTGG24650.015.97088294
GTGGTAT46750.015.7357771
GCAGAGT176850.015.262589
GTATAGG24250.014.71386051
CAGAGTA170550.014.53557510-11
AGAGTAC149200.014.50541810-11
GTGTAGG33050.014.3948011
Produced by FastQC (version 0.11.5)