##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140245_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7328972
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199255502681687	31.0	31.0	34.0	28.0	34.0
2	31.312894359536372	31.0	31.0	34.0	28.0	34.0
3	31.426075853475766	31.0	31.0	34.0	28.0	34.0
4	35.10290433637896	37.0	35.0	37.0	32.0	37.0
5	35.22927635690244	37.0	35.0	37.0	32.0	37.0
6	35.39762206759693	37.0	35.0	37.0	33.0	37.0
7	35.36936899199506	37.0	35.0	37.0	33.0	37.0
8	35.31256293515653	37.0	35.0	37.0	32.0	37.0
9	36.957188948190826	39.0	37.0	39.0	33.0	39.0
10-11	36.95592294526436	39.0	37.0	39.0	33.0	39.0
12-13	36.904592076487674	39.0	37.0	39.0	33.0	39.0
14-15	38.258877779857805	40.0	38.0	41.0	33.0	41.0
16-17	38.26162004712258	40.0	38.0	41.0	33.0	41.0
18-19	38.27648195408578	40.0	38.0	41.0	33.0	41.0
20-21	38.339130818480626	40.0	38.0	41.0	33.0	41.0
22-23	38.323771098164364	40.0	38.0	41.0	33.0	41.0
24-25	38.35413706735736	40.0	38.0	41.0	33.0	41.0
26-27	38.340156780329515	40.0	38.0	41.0	33.0	41.0
28-29	38.28090064670539	40.0	38.0	41.0	33.0	41.0
30-31	38.21189800268147	40.0	38.0	41.0	33.0	41.0
32-33	38.17563696820168	40.0	38.0	41.0	33.0	41.0
34-35	38.10816938712597	40.0	38.0	41.0	32.5	41.0
36-37	38.0233629275336	40.0	38.0	41.0	32.0	41.0
38-39	37.937396244855506	40.0	37.5	41.0	32.0	41.0
40-41	37.89254079217583	40.0	37.0	41.0	32.0	41.0
42-43	37.73300269635624	40.0	37.0	41.0	31.5	41.0
44-45	37.5486674240818	40.0	37.0	41.0	31.0	41.0
46-47	37.40451429163686	40.0	36.0	41.0	31.0	41.0
48-49	37.26978523333781	39.0	36.0	41.0	31.0	41.0
50-51	37.13612201904962	39.0	36.0	40.5	31.0	41.0
52-53	36.98879026244411	39.0	35.5	40.0	30.0	41.0
54-55	36.81349341632759	39.0	35.0	40.0	30.0	41.0
56-57	36.79056930670695	39.0	35.0	40.0	30.0	41.0
58-59	36.85642192076487	39.0	35.0	40.0	30.0	41.0
60-61	37.15623254299645	39.0	35.0	41.0	31.0	41.0
62-63	37.111701203155334	39.0	35.0	41.0	31.0	41.0
64-65	36.88495809322322	38.5	35.0	41.0	31.0	41.0
66-67	36.65027006372098	38.0	35.0	40.0	31.0	41.0
68-69	36.344629910286656	37.0	35.0	40.0	31.0	41.0
70-71	36.06443287457195	37.0	35.0	39.5	30.5	41.0
72-73	35.734530777426954	36.0	34.0	39.0	30.0	41.0
74-75	35.39835735035368	36.0	34.0	39.0	30.0	41.0
76-77	35.068817654364	35.0	34.0	38.5	30.0	40.0
78-79	34.73518697122108	35.0	34.0	37.0	30.0	39.0
80-81	34.41330388409827	35.0	34.0	37.0	29.0	39.0
82-83	34.14902819080504	35.0	34.0	36.0	29.0	39.0
84-85	33.91064306203716	35.0	33.5	36.0	29.0	37.0
86-87	33.71143518338644	35.0	33.0	36.0	29.0	37.0
88-89	33.55327089696244	35.0	33.0	35.0	29.0	36.5
90-91	33.41257915395852	35.0	33.0	35.0	29.0	36.0
92-93	33.32947609631432	35.0	33.0	35.0	29.0	36.0
94-95	33.31337433933539	35.0	33.0	35.0	29.0	36.0
96-97	33.34182217770869	35.0	33.0	35.0	29.0	36.0
98-99	33.461293189911295	35.0	33.0	35.0	29.5	35.0
100	32.73790871482264	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	54.0
17	308.0
18	1294.0
19	4183.0
20	10505.0
21	20326.0
22	31592.0
23	44030.0
24	57048.0
25	70021.0
26	77825.0
27	81555.0
28	93337.0
29	113041.0
30	133605.0
31	159539.0
32	201106.0
33	264553.0
34	362811.0
35	516570.0
36	832898.0
37	1466360.0
38	1882292.0
39	865428.0
40	38688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3310488837998	20.26738538501716	14.472971106998362	23.928594624184672
2	14.260414571434577	20.59560494564382	42.08682523519591	23.057155247725685
3	18.271248487865805	25.628272651442696	29.9347025517401	26.1657763089514
4	12.467190723481991	17.79305765057126	38.81738806702407	30.922363558922683
5	13.606933934695723	37.90649034987757	35.25075362477448	13.235822090652213
6	30.424069502239327	37.28815664123275	19.04705304222409	13.240720814303838
7	27.08952555631068	32.433110345072286	22.769339523509295	17.708024575107743
8	24.399674108119314	37.059162802806625	20.987770048920623	17.553393040153445
9	25.149993914556003	17.90794715324021	21.344926786377993	35.597132145825796
10-11	24.13923549500069	27.093131642266787	28.68512325308712	20.082509609645403
12-13	24.696992862285523	24.569638204841716	28.71770090560507	22.015668027267694
14-15	22.011982334182388	26.15828229832462	26.090960295027017	25.73877507246598
16-17	21.97212663074601	29.166334080645594	26.76162458071989	22.099914707888505
18-19	22.009296259814988	28.78140158595295	27.890232582509217	21.319069571722842
20-21	22.933010024715035	27.789690401327167	27.645125743061932	21.63217383089587
22-23	22.66808439306469	27.727266455282805	27.436592013799473	22.168057137853033
24-25	22.373622680448737	27.885252619277683	27.48973748498206	22.251387215291523
26-27	22.340381485076925	27.998293195949525	27.438531272530547	22.222794046443
28-29	22.25340767135554	28.06950116749578	27.480747813990313	22.19634334715837
30-31	22.521585523770995	27.857589718355293	27.40238043070794	22.218444327165773
32-33	22.250702273251434	27.90062424594449	27.483747861705428	22.364925619098646
34-35	22.396294584267952	27.863379832303576	27.542442973680796	22.197882609747676
36-37	22.371903750152576	27.67178286202133	27.584361365173876	22.371952022652213
38-39	22.220376862534664	27.624129121545206	27.80422793329944	22.351266082620693
40-41	22.37201963959439	27.454178609468222	27.854001957031894	22.31979979390549
42-43	22.123551868970928	27.46989390118335	27.86269515911352	22.543859070732196
44-45	22.06410335471354	27.460572502496426	27.91930477817268	22.556019364617352
46-47	21.928704098585534	27.412408960196537	28.0021917741049	22.656695167113025
48-49	21.794610929616525	27.410448366290545	28.12890455840993	22.666036145683005
50-51	21.579964508453973	27.476000213913988	28.14641084138453	22.797624436247517
52-53	21.665564527526886	27.43238092730126	28.099645517874894	22.802409027296957
54-55	21.618715228913967	27.308076794608805	28.20633094661823	22.866877029858994
56-57	21.598798542834697	27.40072509674446	28.10577787660761	22.894698483813233
58-59	21.598252157891256	27.371853436795995	28.101097230933565	22.928797174379184
60-61	21.628590918977906	27.18068973066362	28.089296933561776	23.101422416796698
62-63	21.647521533636645	27.167157559962895	28.007781262423254	23.17753964397721
64-65	21.7276005308595	27.251568094316447	27.890298904869216	23.130532469954836
66-67	21.60175865952059	27.371501883795794	27.59738212216237	23.42935733452125
68-69	21.69447734675211	27.465269318493945	27.446542141334483	23.393711193419463
70-71	21.71430250223309	27.473616128888565	27.29493229224057	23.517149076637775
72-73	21.919279494644314	27.385091071692795	26.967188609260013	23.728440824402874
74-75	21.950250840028946	27.33430440741731	26.828181592100268	23.887263160453475
76-77	22.245424928328898	27.13116149650723	26.94262280636667	23.6807907687972
78-79	22.310269433808838	26.872126041286613	27.10429003002642	23.713314494878126
80-81	22.474123899465283	26.825756335036026	27.04356964833341	23.656550117165274
82-83	22.426641666303883	26.97549955525375	27.030881268298696	23.566977510143673
84-85	22.554868017072774	26.809805913026146	27.110346585992268	23.52497948390881
86-87	22.24108057238439	27.01915774167208	27.358478328688424	23.381283357255107
88-89	22.323422241835907	27.19657403321223	27.17765259632029	23.30235112863157
90-91	22.34076805515662	27.388820066742237	27.354550245943027	22.915861632158116
92-93	22.04675130389169	27.39076322264522	27.649381559824782	22.91310391363831
94-95	21.815030037801115	27.463778844914977	27.990714596122057	22.730476521161854
96-97	21.94509920606969	27.45789847106568	28.131418846133794	22.465583476730842
98-99	23.30743768340519	29.478448958658447	28.18138900956883	19.032724348367527
100	22.718700612242188	30.606216051104298	21.887595953358037	24.787487383295485
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3059.0
1	2235.5
2	1526.0
3	1609.5
4	1523.0
5	1532.0
6	1773.5
7	2135.5
8	2409.0
9	2622.5
10	2896.0
11	3110.0
12	3363.0
13	3790.0
14	4310.5
15	5005.0
16	5955.5
17	7089.0
18	8393.0
19	10146.5
20	12502.0
21	15818.0
22	20436.0
23	26226.5
24	33393.0
25	42298.5
26	53377.0
27	65403.5
28	79213.0
29	95515.5
30	111176.0
31	126288.5
32	141674.0
33	156352.5
34	171267.0
35	184946.0
36	198955.0
37	214065.5
38	226939.0
39	238305.5
40	250868.5
41	262271.0
42	270763.0
43	279126.5
44	287426.5
45	296327.0
46	302166.0
47	306222.0
48	308742.5
49	307938.0
50	305919.5
51	299699.0
52	288148.5
53	273116.0
54	258093.0
55	239371.5
56	216793.5
57	193217.5
58	170119.0
59	146937.5
60	121230.0
61	97970.0
62	77738.5
63	60005.0
64	45911.0
65	35184.5
66	27453.0
67	21739.0
68	17047.0
69	13379.0
70	10489.0
71	7979.5
72	6183.5
73	4893.0
74	3886.5
75	3014.0
76	2263.5
77	1709.5
78	1276.5
79	967.5
80	742.5
81	567.5
82	451.5
83	316.5
84	219.5
85	167.0
86	132.5
87	104.5
88	66.0
89	42.0
90	32.0
91	24.5
92	20.5
93	14.5
94	8.0
95	6.5
96	7.5
97	6.5
98	4.5
99	6.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011270339141696816
3	0.021558275840049598
4	0.019034047339790628
5	0.009864957868579658
6	0.014913414869097603
7	0.01680999736388678
8	0.002196761019144295
9	1.0915582703822582E-4
10-11	1.0233358784833671E-4
12-13	0.0030768298746399908
14-15	2.1831165407645164E-4
16-17	5.594236135709074E-4
18-19	4.093343513933468E-5
20-21	8.1910122207172E-5
22-23	0.0
24-25	3.418216537317935E-5
26-27	0.0026005159286733226
28-29	0.003542412406144914
30-31	0.003108375252941463
32-33	0.004363676045913019
34-35	0.006594252413802693
36-37	9.861284823351633E-4
38-39	0.0013891840617046532
40-41	6.442576759145583E-4
42-43	4.858478760327217E-5
44-45	4.171429338177012E-5
46-47	0.00440174228202896
48-49	6.559161543775425E-4
50-51	3.145784020256053E-4
52-53	0.004026929583848693
54-55	2.3151730690088547E-4
56-57	9.136441152358238E-5
58-59	6.829001657687351E-4
60-61	4.2317862158027596E-5
62-63	7.067449476217185E-6
64-65	1.4232919304827216E-5
66-67	0.0016878962111901486
68-69	0.0
70-71	3.736455623105626E-4
72-73	0.0014803793116695118
74-75	2.243811847401348E-4
76-77	1.5127264542047406E-5
78-79	3.0627475034970835E-5
80-81	0.0030347951292081492
82-83	1.1808453656227889E-4
84-85	3.5134955359841433E-4
86-87	0.012613280389980582
88-89	5.356649868762077E-4
90-91	7.048591225762601E-4
92-93	4.277226557333912E-5
94-95	2.445820062939685E-4
96-97	7.178469401370388E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2606.0
20-21	5179.0
22-23	5769.0
24-25	7032.0
26-27	8743.0
28-29	10025.0
30-31	11012.0
32-33	11406.0
34-35	13307.0
36-37	15266.0
38-39	16589.0
40-41	15264.0
42-43	11893.0
44-45	12631.0
46-47	13388.0
48-49	13231.0
50-51	12890.0
52-53	12717.0
54-55	12603.0
56-57	12223.0
58-59	12647.0
60-61	13524.0
62-63	37729.0
64-65	63210.0
66-67	67305.0
68-69	68530.0
70-71	70126.0
72-73	69341.0
74-75	72968.0
76-77	78649.0
78-79	86634.0
80-81	90342.0
82-83	89888.0
84-85	92193.0
86-87	98668.0
88-89	107071.0
90-91	112266.0
92-93	118388.0
94-95	134863.0
96-97	285776.0
98-99	516072.0
100-101	4819008.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.65041019461655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.62082551762093	72.41897170622295
2	8.509074220340402	14.065569493751426
3	2.1827410324675043	5.412133250461801
4	0.7917134972782659	2.6174178122665244
5	0.3607952051932058	1.4909935852734653
6	0.190722876702357	0.9457994395768251
7	0.10630019521654245	0.6150028316890533
8	0.06778412559225556	0.4481908627906663
9	0.0402278540529091	0.29923637748498944
>10	0.1281049107053187	1.569093732972855
>50	0.0013985207691687772	0.07332837887277742
>100	3.120440611384724E-4	0.04426252863660664
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.093343513933468E-5
2	0.0	4.093343513933468E-5	0.0	0.0	4.093343513933468E-5
3	0.0	4.093343513933468E-5	0.0	1.3644478379778228E-5	4.093343513933468E-5
4	0.0	1.2280030541800404E-4	0.0	2.7288956759556455E-5	4.093343513933468E-5
5	0.0	1.364447837977823E-4	0.0	4.093343513933468E-5	4.093343513933468E-5
6	0.0	1.500892621775605E-4	0.0	6.822239189889114E-5	5.457791351911291E-5
7	0.0	1.6373374055733873E-4	0.0	6.822239189889114E-5	5.457791351911291E-5
8	0.0	1.7737821893711695E-4	0.0	8.186687027866937E-5	6.822239189889114E-5
9	0.0	3.547564378742339E-4	0.0	1.0915582703822582E-4	6.822239189889114E-5
10-11	1.3644478379778228E-5	3.547564378742339E-4	0.0	1.910226973168952E-4	6.822239189889114E-5
12-13	1.3644478379778228E-5	3.547564378742339E-4	0.0	2.38778371646119E-4	6.822239189889114E-5
14-15	1.3644478379778228E-5	4.912012216720162E-4	0.0	2.933562851652319E-4	7.504463108878026E-5
16-17	1.3644478379778228E-5	5.184901784315727E-4	0.0	3.342897203045666E-4	8.186687027866937E-5
18-19	1.3644478379778228E-5	5.594236135709074E-4	0.0	3.956898730135686E-4	1.0915582703822582E-4
20-21	1.3644478379778228E-5	6.00357048710242E-4	0.0	5.253124176214618E-4	1.0915582703822582E-4
22-23	1.3644478379778228E-5	6.412904838495767E-4	0.0	0.001071091552812591	1.1597806622811493E-4
24-25	2.046671756966734E-5	6.958683973686897E-4	0.0	0.0024832950651196375	1.2280030541800404E-4
26-27	2.7288956759556455E-5	7.231573541282461E-4	0.0	0.006017214965482198	1.2280030541800404E-4
28-29	2.7288956759556455E-5	7.231573541282461E-4	0.0	0.026688599710846216	1.2280030541800404E-4
30-31	2.7288956759556455E-5	8.050242244069155E-4	0.0	0.0905584030065881	1.2962254460789315E-4
32-33	2.7288956759556455E-5	8.118464635968046E-4	0.0	0.23164230945349495	1.500892621775605E-4
34-35	2.7288956759556455E-5	9.073578122552521E-4	0.0	0.4257827700801695	1.500892621775605E-4
36-37	2.7288956759556455E-5	0.0010096914001035888	0.0	0.7232733321944742	1.500892621775605E-4
38-39	2.7288956759556455E-5	0.0010506248352429237	0.0	1.1357663803327398	1.500892621775605E-4
40-41	3.4111195949445566E-5	0.0011870696190407059	0.0	1.5613922389115418	1.500892621775605E-4
42-43	4.093343513933468E-5	0.001262114250129486	0.0	2.022091229165564	1.5691150136744962E-4
44-45	4.093343513933468E-5	0.0014531369474463812	0.0	2.566758339368741	1.6373374055733873E-4
46-47	4.093343513933468E-5	0.0015964039704340527	0.0	3.182588226561651	1.6373374055733873E-4
48-49	4.093343513933468E-5	0.001610048448813831	0.0	3.796016685559721	1.7737821893711695E-4
50-51	4.093343513933468E-5	0.0018215378637003936	0.0	4.4015245248583295	1.7737821893711695E-4
52-53	4.093343513933468E-5	0.001842004581270061	0.0	4.982560992182805	1.910226973168952E-4
54-55	4.093343513933468E-5	0.0018556490596498391	0.0	5.579240853969697	2.0466717569667342E-4
56-57	4.093343513933468E-5	0.0018692935380296173	0.0	6.232033633093427	2.1148941488656253E-4
58-59	4.093343513933468E-5	0.0018761157772195064	0.0	6.905477330244951	2.1831165407645164E-4
60-61	4.093343513933468E-5	0.0019579826474981758	0.0	7.5675688213845	2.1831165407645164E-4
62-63	4.093343513933468E-5	0.0020057383218273993	0.0	8.183207685880094	2.1831165407645164E-4
64-65	4.093343513933468E-5	0.002333205802942077	0.0	8.342493326485624	2.1831165407645164E-4
66-67	4.093343513933468E-5	0.0023468502813218553	0.0	8.345638378752163	2.3195613245622986E-4
68-69	4.093343513933468E-5	0.0023946059556510793	0.0	8.34827858531865	2.456006108360081E-4
70-71	4.093343513933468E-5	0.002442361629980303	0.0	8.350577679925642	2.456006108360081E-4
72-73	4.093343513933468E-5	0.002449183869170192	0.0	8.352706218552889	2.456006108360081E-4
74-75	4.093343513933468E-5	0.002503761782689305	0.0	8.354834757180134	2.456006108360081E-4
76-77	4.093343513933468E-5	0.0026060953705376415	0.0	8.356608539369503	2.456006108360081E-4
78-79	4.093343513933468E-5	0.0026606732840567544	0.0	8.357870653619635	2.456006108360081E-4
80-81	4.093343513933468E-5	0.002687962240816311	0.0	8.358552877538624	2.660673284056754E-4
82-83	4.093343513933468E-5	0.002708428958385978	0.0	8.359030434281916	3.00178524355121E-4
84-85	4.093343513933468E-5	0.0027288956759556455	0.0	8.359187345783283	3.00178524355121E-4
86-87	4.093343513933468E-5	0.00276982911109498	0.0	8.359221456979233	3.00178524355121E-4
88	4.093343513933468E-5	0.0028789849381332063	0.0	8.359221456979233	3.00178524355121E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5980	0.0	39.041637	1
GTATCAA	9860	0.0	29.21825	1
TCAACGC	10255	0.0	26.416304	4
ATCAACG	10500	0.0	25.884886	3
CAACGCA	10530	0.0	25.724136	5
AACGCAG	11175	0.0	24.316225	6
TATCAAC	11535	0.0	24.173496	2
GTACATG	22640	0.0	21.441666	1
TACATGG	22770	0.0	20.390644	2
ACGCAGA	13380	0.0	20.209793	7
CGCAGAG	13900	0.0	19.324108	8
ACATGGG	23150	0.0	19.29467	3
CATGGGG	17365	0.0	16.614952	4
TCGGTGG	2465	0.0	15.970882	94
GTGGTAT	4675	0.0	15.735777	1
GCAGAGT	17685	0.0	15.26258	9
GTATAGG	2425	0.0	14.7138605	1
CAGAGTA	17055	0.0	14.535575	10-11
AGAGTAC	14920	0.0	14.505418	10-11
GTGTAGG	3305	0.0	14.394801	1
>>END_MODULE