FastQCFastQC Report
Fri 27 May 2016
SRR2140244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9713275
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73750.036.575691
GTATCAA121650.031.1055551
TCAACGC130950.027.2291854
ATCAACG135250.026.395593
CAACGCA136550.026.1108845
AACGCAG144250.024.7146786
TATCAAC150400.024.2111512
ACGCAGA172550.020.684677
GTACATG289100.019.9090061
CGCAGAG180850.019.6860398
TACATGG291800.018.8190612
ACATGGG301400.017.6209583
CATGGGG229450.015.6131674
GCAGAGT226950.015.446729
AGAGTAC190500.014.73745510-11
CAGAGTA217000.014.12617510-11
GTGGTAT54950.014.0473271
TATACCG7750.012.9898425
TATACTG52950.012.6750125
GAGTACA222850.012.5938061
Produced by FastQC (version 0.11.5)