##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140244_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9713275
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979928705817553	31.0	31.0	34.0	28.0	34.0
2	31.09237862615853	31.0	31.0	34.0	28.0	34.0
3	31.196963125207514	31.0	31.0	34.0	28.0	34.0
4	34.928631074483114	37.0	35.0	37.0	32.0	37.0
5	35.06143128862304	37.0	35.0	37.0	32.0	37.0
6	35.28257595918987	37.0	35.0	37.0	32.0	37.0
7	35.26842995796989	37.0	35.0	37.0	32.0	37.0
8	35.20242904684568	37.0	35.0	37.0	32.0	37.0
9	36.8490553392136	39.0	37.0	39.0	33.0	39.0
10-11	36.845213483608774	39.0	37.0	39.0	32.5	39.0
12-13	36.79301450849482	39.0	37.0	39.0	32.0	39.0
14-15	38.11273478821509	40.0	38.0	41.0	33.0	41.0
16-17	38.105943927254195	40.0	38.0	41.0	32.5	41.0
18-19	38.10746447516414	40.0	38.0	41.0	32.0	41.0
20-21	38.15396865610633	40.0	38.0	41.0	32.0	41.0
22-23	38.13556191505174	40.0	38.0	41.0	32.0	41.0
24-25	38.16212156235939	40.0	38.0	41.0	32.0	41.0
26-27	38.13854841219312	40.0	38.0	41.0	32.0	41.0
28-29	38.076015937621236	40.0	38.0	41.0	32.0	41.0
30-31	38.01055348580414	40.0	38.0	41.0	32.0	41.0
32-33	37.97089537960245	40.0	38.0	41.0	32.0	41.0
34-35	37.90586178367599	40.0	37.0	41.0	32.0	41.0
36-37	37.822413652613385	40.0	37.0	41.0	32.0	41.0
38-39	37.732185008417595	40.0	37.0	41.0	31.0	41.0
40-41	37.680742291382856	40.0	37.0	41.0	31.0	41.0
42-43	37.51956114819974	40.0	37.0	41.0	31.0	41.0
44-45	37.30922046143587	40.0	36.0	41.0	31.0	41.0
46-47	37.14718138262322	39.0	36.0	41.0	30.0	41.0
48-49	37.005265879353544	39.0	36.0	40.0	30.0	41.0
50-51	36.865538020904225	39.0	35.0	40.0	30.0	41.0
52-53	36.70738181440309	39.0	35.0	40.0	30.0	41.0
54-55	36.5214477139955	38.5	35.0	40.0	30.0	41.0
56-57	36.49040331086185	38.5	35.0	40.0	29.5	41.0
58-59	36.54086025149427	38.5	35.0	40.0	30.0	41.0
60-61	36.84805715241926	39.0	35.0	40.0	30.5	41.0
62-63	36.80202877854384	39.0	35.0	40.0	31.0	41.0
64-65	36.58030786541721	38.0	35.0	40.0	30.5	41.0
66-67	36.35496164977642	37.5	35.0	40.0	30.0	41.0
68-69	36.06500897436919	37.0	34.5	40.0	30.0	41.0
70-71	35.81620373548316	36.5	34.0	39.5	30.0	41.0
72-73	35.51730286510159	36.0	34.0	39.0	30.0	41.0
74-75	35.215081832053116	36.0	34.0	39.0	30.0	40.5
76-77	34.92201119481831	35.0	34.0	37.5	30.0	40.0
78-79	34.62829152531627	35.0	34.0	37.0	29.5	39.0
80-81	34.34259506404487	35.0	34.0	37.0	29.0	39.0
82-83	34.1078390792012	35.0	34.0	36.0	29.0	38.5
84-85	33.896973941374114	35.0	33.0	36.0	29.0	37.0
86-87	33.71442512533382	35.0	33.0	35.5	29.0	37.0
88-89	33.5802436389652	35.0	33.0	35.0	29.0	36.5
90-91	33.463681588726175	35.0	33.0	35.0	29.0	36.0
92-93	33.40182454878175	35.0	33.0	35.0	29.0	36.0
94-95	33.38696235692077	35.0	33.0	35.0	29.0	36.0
96-97	33.40206355842536	35.0	33.0	35.0	29.0	36.0
98-99	33.45392426771552	35.0	33.0	35.0	29.5	35.0
100	32.73267785779693	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	44.0
17	301.0
18	1641.0
19	5681.0
20	14491.0
21	28796.0
22	45826.0
23	63497.0
24	83745.0
25	102713.0
26	114429.0
27	119292.0
28	134523.0
29	159760.0
30	185202.0
31	221979.0
32	277399.0
33	357457.0
34	485061.0
35	686702.0
36	1085376.0
37	1849279.0
38	2330019.0
39	1310030.0
40	50025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.284767496029914	20.11219696755214	14.434740085089736	24.16829545132821
2	14.383227221909783	20.535448601427618	41.69718695757197	23.384137219090626
3	18.246876722897085	25.443507305135128	29.89818675797648	26.411429213991312
4	12.633986419304588	17.710024639002775	38.422871106959306	31.23311783473333
5	13.776529640402584	37.711827846276606	35.12722592602126	13.384416587299544
6	30.743542331698436	37.02694064688607	18.851111343127872	13.378405678287617
7	27.317217576108288	32.32237623843935	22.572967785646767	17.7874383998056
8	24.62490278574813	36.70815298378002	20.93133775664614	17.73560647382571
9	25.38274466749797	17.698602653031976	21.04072328313119	35.877929396338864
10-11	24.238359979106924	26.90051796684462	28.586629430995224	20.274492623053224
12-13	24.7968369855986	24.378089476458026	28.584080964372227	22.24099257357115
14-15	22.12719515099752	25.908754279139472	25.90839394680805	26.055656623054958
16-17	22.184401519247775	28.978018430124365	26.583294451648825	22.254285598979042
18-19	22.313396990891178	28.56391528932905	27.602463000505136	21.520224719274637
20-21	23.126650383220355	27.61982876061258	27.411518702038222	21.84200215412884
22-23	22.908601439941307	27.497401429054218	27.190279313643234	22.403717817361237
24-25	22.635672057533725	27.638415122131	27.257265096748682	22.468647723586592
26-27	22.544159280744918	27.75824211016202	27.20952911746521	22.488069491627854
28-29	22.500976002924155	27.791214468359428	27.26214768648122	22.445661842235197
30-31	22.761027990001274	27.634639091032565	27.19005663939585	22.414276279570316
32-33	22.514167895732065	27.72773619541664	27.22056246906106	22.537533439790234
34-35	22.64619227499921	27.737527742569	27.230255181535345	22.386024800896447
36-37	22.65072176843099	27.601767382098046	27.262807620196405	22.48470322927456
38-39	22.511015041593314	27.67416647726418	27.356297626146674	22.458520854995832
40-41	22.6926175788287	27.544168235235013	27.31034101442268	22.452873171513613
42-43	22.575058546667655	27.63061644027051	27.246284298484614	22.54804071457722
44-45	22.597106366701304	27.65529268986622	27.223425810036428	22.524175133396053
46-47	22.598248086146246	27.60369835050353	27.167165414447947	22.63088814890228
48-49	22.530298205390853	27.592154769705846	27.28413127767538	22.593415747227922
50-51	22.404728079506455	27.675233620903562	27.338072637868187	22.5819656617218
52-53	22.60768553564393	27.60156568451065	27.195094413651844	22.59565436619358
54-55	22.632684055633035	27.506691842452973	27.235836857520372	22.624787244393616
56-57	22.54561147922708	27.599414803529836	27.271501810073573	22.58347190716951
58-59	22.553224038876014	27.59899088918904	27.316617000003163	22.531168071931784
60-61	22.578490663126246	27.563468846209467	27.349498701671305	22.508541788992982
62-63	22.58052050103209	27.632394680052542	27.27777842934885	22.50930638956652
64-65	22.560712146124647	27.573928024982962	27.28457750287402	22.580782326018365
66-67	22.575680704850413	27.587156034134342	27.1980356290316	22.639127631983644
68-69	22.512307658236956	27.568440454725934	27.264659337355994	22.654592549681116
70-71	22.4655842367011	27.416134236621552	27.35341993931601	22.76486158736134
72-73	22.52122226222593	27.454285693249226	27.306442468815412	22.718049575709436
74-75	22.393842196592786	27.5837688274275	27.32996872173427	22.692420254245445
76-77	22.541987527725585	27.513621782301474	27.235505649364416	22.70888504060852
78-79	22.49001775893767	27.51969611047707	27.22072657438779	22.76955955619747
80-81	22.49030483319271	27.544254218261944	27.2743533730898	22.691087575455548
82-83	22.48325652132386	27.558103623266746	27.354169574887248	22.604470280522147
84-85	22.55818190195588	27.563620033729563	27.321045231367247	22.55715283294731
86-87	22.437504651314597	27.586747731587064	27.373898197269792	22.601849419828547
88-89	22.472619858318147	27.58377035350172	27.327272878358293	22.61633690982184
90-91	22.477877573580212	27.66672654414813	27.30368246510985	22.551713417161807
92-93	22.44888485156015	27.614241653760164	27.36258414471439	22.574289349965294
94-95	22.31108629798261	27.69696878107977	27.432940397547377	22.55900452339024
96-97	22.388523918894133	27.86015440436763	27.42165562641042	22.329666050327816
98-99	23.489913158209166	29.789684804466148	27.603235848169227	19.117166189155455
100	22.434487519599795	30.799966665051343	21.29957336966865	25.46597244568021
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3340.0
1	2497.5
2	1793.0
3	1899.5
4	1831.5
5	1896.5
6	2203.5
7	2566.0
8	2804.5
9	3107.0
10	3553.0
11	3938.0
12	4356.5
13	4985.0
14	5748.0
15	6751.0
16	8034.5
17	9559.5
18	11652.5
19	14414.0
20	18124.5
21	23086.5
22	29669.0
23	38411.5
24	49446.5
25	63454.5
26	80325.0
27	98650.5
28	118889.0
29	141677.5
30	163630.5
31	184897.0
32	207295.0
33	228414.0
34	249463.5
35	269576.0
36	289337.0
37	308523.0
38	324778.5
39	339975.5
40	356395.0
41	371207.5
42	381250.5
43	391412.5
44	401339.5
45	410824.5
46	417868.0
47	421062.5
48	421890.5
49	419500.5
50	416360.5
51	408634.0
52	396175.0
53	379841.0
54	360435.0
55	338671.5
56	314678.5
57	289750.5
58	260909.0
59	228752.5
60	195012.5
61	162375.5
62	132602.0
63	105895.5
64	82857.5
65	64558.0
66	50173.0
67	39294.5
68	31176.5
69	24533.5
70	19479.5
71	15220.0
72	11700.5
73	8919.5
74	6705.0
75	5144.5
76	3929.0
77	2964.0
78	2289.0
79	1764.5
80	1322.5
81	991.0
82	766.0
83	528.0
84	356.0
85	263.0
86	207.0
87	161.0
88	115.0
89	79.0
90	55.5
91	46.0
92	35.5
93	22.5
94	12.5
95	8.5
96	8.0
97	6.5
98	4.0
99	4.5
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011911533442633921
3	0.02224790299873112
4	0.018974032960046947
5	0.010552568520915963
6	0.013723486671591198
7	0.01497949970530022
8	0.0019869714385724693
9	1.1324707680982984E-4
10-11	8.75091048075958E-5
12-13	0.002697339465834129
14-15	2.0075618161742562E-4
16-17	4.838738736420003E-4
18-19	5.147594400446811E-6
20-21	6.180845035862035E-5
22-23	0.0
24-25	2.0638119297719884E-5
26-27	0.0023139924541119286
28-29	0.003496779677999354
30-31	0.0026178057966514375
32-33	0.0037745315304659544
34-35	0.005995918079164592
36-37	9.597264685165404E-4
38-39	0.0013093018026710073
40-41	5.794875033785716E-4
42-43	7.49602389475411E-5
44-45	5.3967140703237175E-5
46-47	0.0042520108581439895
48-49	7.69050295944287E-4
50-51	3.048262080484317E-4
52-53	0.00441645171870691
54-55	3.1023090768487083E-4
56-57	1.6515502361716836E-4
58-59	6.383824515910562E-4
60-61	5.807777322383505E-6
62-63	0.0
64-65	1.184430658987608E-5
66-67	0.0018792529694142003
68-69	0.0
70-71	3.120713417115718E-4
72-73	0.0016457690093899784
74-75	1.2509539305566701E-4
76-77	6.32570885102671E-6
78-79	3.200614927744838E-5
80-81	0.0031495865001205073
82-83	1.7055700638342395E-4
84-85	3.98347706825117E-4
86-87	0.013228655768934101
88-89	6.947411094933785E-4
90-91	8.497924192021858E-4
92-93	3.506247677549195E-5
94-95	1.4969593547734507E-4
96-97	6.563256962302985E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3982.0
20-21	7571.0
22-23	8487.0
24-25	9970.0
26-27	13323.0
28-29	17784.0
30-31	27543.0
32-33	33091.0
34-35	43424.0
36-37	60806.0
38-39	66080.0
40-41	66097.0
42-43	70584.0
44-45	80271.0
46-47	81834.0
48-49	81097.0
50-51	78190.0
52-53	78157.0
54-55	83815.0
56-57	86070.0
58-59	84707.0
60-61	83515.0
62-63	82742.0
64-65	87169.0
66-67	90714.0
68-69	92083.0
70-71	90832.0
72-73	87701.0
74-75	88361.0
76-77	92288.0
78-79	96098.0
80-81	93906.0
82-83	90918.0
84-85	92106.0
86-87	95850.0
88-89	102512.0
90-91	106344.0
92-93	111708.0
94-95	135346.0
96-97	337032.0
98-99	609456.0
100-101	5963711.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.77075605158961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07876424964113	77.26123713884621
2	6.978881770268433	11.971679316611592
3	1.677545134742026	4.3165294355246795
4	0.6017763520190057	2.0645925074655063
5	0.2554009773003329	1.0952967459682215
6	0.14393279211711713	0.7407134640300848
7	0.07932887310410432	0.4762868196001747
8	0.051719443201733106	0.35488125967839157
9	0.029569858401329634	0.22826062002784425
>10	0.10065356843803393	1.318142098765118
>50	0.002086228924563636	0.12177251633495181
>100	3.215639045365237E-4	0.041079104051828144
>500	1.9187937655147195E-5	0.009528973095368366
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.088556640268087E-5	0.0	0.0	0.0
3	0.0	3.088556640268087E-5	0.0	0.0	0.0
4	0.0	4.118075520357449E-5	0.0	0.0	1.0295188800893622E-5
5	0.0	4.118075520357449E-5	0.0	0.0	2.0590377601787245E-5
6	8.236151040714898E-5	4.118075520357449E-5	0.0	0.0	2.0590377601787245E-5
7	8.236151040714898E-5	5.147594400446811E-5	0.0	0.0	2.0590377601787245E-5
8	9.26566992080426E-5	5.147594400446811E-5	0.0	0.0	3.088556640268087E-5
9	1.0295188800893622E-4	8.236151040714898E-5	0.0	0.0	4.118075520357449E-5
10-11	1.0295188800893622E-4	8.236151040714898E-5	0.0	0.0	4.118075520357449E-5
12-13	1.0295188800893622E-4	8.236151040714898E-5	0.0	0.0	5.662353840491492E-5
14-15	1.0295188800893622E-4	1.389850488120639E-4	0.0	0.0	9.26566992080426E-5
16-17	1.389850488120639E-4	1.441326432125107E-4	0.0	0.0	1.1324707680982984E-4
18-19	1.4928023761295753E-4	1.4928023761295753E-4	0.0	0.0	1.441326432125107E-4
20-21	1.5957542641385113E-4	1.750182096151916E-4	0.0	1.5442783201340434E-5	1.750182096151916E-4
22-23	1.7501820961519156E-4	2.419369368210001E-4	0.0	5.662353840491492E-5	1.9560858721697882E-4
24-25	1.853133984160852E-4	2.6252731442278737E-4	0.0	1.9560858721697882E-4	2.1619896481876608E-4
26-27	2.1619896481876608E-4	2.676749088232342E-4	0.0	5.302022232460215E-4	2.2649415361965968E-4
28-29	2.1619896481876608E-4	2.676749088232342E-4	0.0	0.002707634654635023	2.316417480201065E-4
30-31	2.2649415361965968E-4	2.9856047522591506E-4	0.0	0.010017218703269494	2.367893424205533E-4
32-33	2.367893424205533E-4	2.9856047522591506E-4	0.0	0.02713811767915559	2.6252731442278737E-4
34-35	2.419369368210001E-4	3.345936360290427E-4	0.0	0.04579814738077528	2.779700976241278E-4
36-37	2.4708453122144694E-4	3.757743912326172E-4	0.0	0.06799457443550193	2.882652864250214E-4
38-39	2.5223212562189377E-4	3.9121717443395764E-4	0.0	0.09049985715425539	3.0885566402680866E-4
40-41	2.676749088232342E-4	4.118075520357449E-4	0.0	0.10895913067425765	3.140032584272555E-4
42-43	2.779700976241278E-4	4.221027408366385E-4	0.0	0.12892150175919037	3.2429844722814914E-4
44-45	3.0370806962636183E-4	5.250546288455747E-4	0.0	0.1439988057580991	3.294460416285959E-4
46-47	3.191508528277023E-4	6.177113280536173E-4	0.0	0.1583708893241466	3.397412304294895E-4
48-49	3.294460416285959E-4	6.177113280536173E-4	0.0	0.17010740455716533	3.603316080312768E-4
50-51	3.603316080312768E-4	6.743348664585323E-4	0.0	0.18032023184765178	3.8092198563306404E-4
52-53	3.603316080312768E-4	6.79482460858979E-4	0.0	0.18937485039803775	4.0665995763529807E-4
54-55	3.603316080312768E-4	7.052204328612131E-4	0.0	0.1979919234243857	4.3239792963753216E-4
56-57	3.603316080312768E-4	7.309584048634472E-4	0.0	0.2060839418218881	4.4269311843842576E-4
58-59	3.603316080312768E-4	7.36105999263894E-4	0.0	0.21371782431775072	4.581359016397662E-4
60-61	3.8092198563306404E-4	7.515487824652344E-4	0.0	0.22026556439511905	4.735786848411066E-4
62-63	4.3239792963753216E-4	7.721391600670217E-4	0.0	0.22609264125642484	4.838738736420003E-4
64-65	4.3239792963753216E-4	8.699434536755111E-4	0.0	0.2299481894623595	4.838738736420003E-4
66-67	4.581359016397662E-4	8.750910480759579E-4	0.0	0.23305733648022936	5.044642512437875E-4
68-69	4.63283496040213E-4	8.905338312772983E-4	0.0	0.23605838401568985	5.404974120469152E-4
70-71	4.735786848411067E-4	9.059766144786387E-4	0.0	0.23851893413910344	5.559401952482556E-4
72-73	5.044642512437875E-4	9.214193976799792E-4	0.0	0.24055738152168038	5.919733560513832E-4
74-75	5.044642512437875E-4	9.471573696822133E-4	0.0	0.24238992512823945	6.228589224540642E-4
76-77	5.044642512437875E-4	9.831905304853409E-4	0.0	0.24427394467880298	6.485968944562982E-4
78-79	5.096118456442343E-4	9.986333136866813E-4	0.0	0.2456277620061205	6.485968944562982E-4
80-81	5.147594400446811E-4	0.0010037809080871282	0.0	0.24625062092857455	6.588920832571918E-4
82-83	5.147594400446811E-4	0.001008928502487575	0.0	0.24667787126381163	6.897776496598727E-4
84-85	5.302022232460215E-4	0.001008928502487575	0.0	0.24678082315182057	6.897776496598727E-4
86-87	5.404974120469152E-4	0.0010192236912884686	0.0	0.24685288947342682	7.1036802726166E-4
88	5.456450064473619E-4	0.001039814068890256	0.0	0.24687862744542907	7.412535936643408E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7375	0.0	36.57569	1
GTATCAA	12165	0.0	31.105555	1
TCAACGC	13095	0.0	27.229185	4
ATCAACG	13525	0.0	26.39559	3
CAACGCA	13655	0.0	26.110884	5
AACGCAG	14425	0.0	24.714678	6
TATCAAC	15040	0.0	24.211151	2
ACGCAGA	17255	0.0	20.68467	7
GTACATG	28910	0.0	19.909006	1
CGCAGAG	18085	0.0	19.686039	8
TACATGG	29180	0.0	18.819061	2
ACATGGG	30140	0.0	17.620958	3
CATGGGG	22945	0.0	15.613167	4
GCAGAGT	22695	0.0	15.44672	9
AGAGTAC	19050	0.0	14.737455	10-11
CAGAGTA	21700	0.0	14.126175	10-11
GTGGTAT	5495	0.0	14.047327	1
TATACCG	775	0.0	12.989842	5
TATACTG	5295	0.0	12.675012	5
GAGTACA	22285	0.0	12.593806	1
>>END_MODULE