FastQCFastQC Report
Fri 27 May 2016
SRR2140243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789717
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61700.034.8108441
GTATCAA101400.031.9394021
TCAACGC104150.029.105774
ATCAACG107500.028.1601493
CAACGCA110300.027.4037955
TATCAAC119850.025.9995252
AACGCAG116600.025.9562366
ACGCAGA139550.021.6265497
CGCAGAG145000.020.6370628
GTACATG231250.020.3675791
TACATGG238450.019.3624942
ACATGGG238650.018.7082373
CATGGGG179350.016.831244
GCAGAGT181050.016.2938169
GTGGTAT45200.015.2470261
AGAGTAC153450.014.9142110-11
CAGAGTA176550.014.26747710-11
GTATAAG30900.013.6828791
TGGTATC45600.013.4443292
ATGGGGG108000.013.3478355
Produced by FastQC (version 0.11.5)