##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7789717
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.412134356100484	31.0	31.0	34.0	30.0	34.0
2	31.53028036833687	31.0	31.0	34.0	30.0	34.0
3	31.642128590807598	31.0	31.0	34.0	30.0	34.0
4	35.30366392001147	37.0	35.0	37.0	33.0	37.0
5	35.425898912630586	37.0	35.0	37.0	33.0	37.0
6	35.57740441661745	37.0	35.0	37.0	33.0	37.0
7	35.550060034273386	37.0	35.0	37.0	33.0	37.0
8	35.49458292258884	37.0	35.0	37.0	33.0	37.0
9	37.17295211109723	39.0	37.0	39.0	34.0	39.0
10-11	37.175543732846776	39.0	37.0	39.0	34.0	39.0
12-13	37.128244260991764	39.0	37.0	39.0	33.0	39.0
14-15	38.519222328616046	40.0	38.0	41.0	34.0	41.0
16-17	38.5180962928435	40.0	38.0	41.0	33.5	41.0
18-19	38.53083873265229	40.0	38.0	41.0	33.5	41.0
20-21	38.586504908220654	40.0	38.0	41.0	34.0	41.0
22-23	38.56482814192161	40.0	38.0	41.0	34.0	41.0
24-25	38.59464097099726	40.0	38.0	41.0	34.0	41.0
26-27	38.58401359363695	40.0	38.0	41.0	34.0	41.0
28-29	38.521571269605474	40.0	38.0	41.0	33.0	41.0
30-31	38.454427257072936	40.0	38.0	41.0	33.0	41.0
32-33	38.41919000263071	40.0	38.0	41.0	33.0	41.0
34-35	38.350406504909785	40.0	38.0	41.0	33.0	41.0
36-37	38.266095121257244	40.0	38.0	41.0	33.0	41.0
38-39	38.17765993579181	40.0	38.0	41.0	33.0	41.0
40-41	38.13219914310089	40.0	38.0	41.0	33.0	41.0
42-43	37.97574303148423	40.0	37.5	41.0	32.5	41.0
44-45	37.787975860976914	40.0	37.0	41.0	32.0	41.0
46-47	37.64124742745152	40.0	37.0	41.0	32.0	41.0
48-49	37.50776449060184	40.0	36.5	41.0	31.0	41.0
50-51	37.37615150302419	39.5	36.0	41.0	31.0	41.0
52-53	37.22658441437362	39.0	36.0	41.0	31.0	41.0
54-55	37.048408336836246	39.0	35.5	40.0	31.0	41.0
56-57	37.01703030163324	39.0	35.5	40.0	30.5	41.0
58-59	37.065077456840285	39.0	35.0	41.0	31.0	41.0
60-61	37.347745562091575	39.0	35.5	41.0	31.5	41.0
62-63	37.2932210230382	39.0	35.0	41.0	32.0	41.0
64-65	37.071424475560036	39.0	35.0	41.0	31.0	41.0
66-67	36.84276941362851	38.0	35.0	40.5	31.0	41.0
68-69	36.54912769957055	37.5	35.0	40.0	31.0	41.0
70-71	36.291437915292576	37.0	35.0	40.0	31.0	41.0
72-73	35.984105634124475	36.5	35.0	39.0	31.0	41.0
74-75	35.67184721710661	36.0	34.5	39.0	31.0	41.0
76-77	35.364746407177805	36.0	34.0	38.5	30.5	40.0
78-79	35.05958329064302	35.0	34.0	37.0	30.0	39.5
80-81	34.76203296067992	35.0	34.0	37.0	30.0	39.0
82-83	34.50940752036219	35.0	34.0	36.5	30.0	39.0
84-85	34.28208526829552	35.0	34.0	36.0	30.0	37.5
86-87	34.0890738390101	35.0	34.0	36.0	30.0	37.0
88-89	33.93656093003881	35.0	34.0	35.5	30.0	37.0
90-91	33.811001488912254	35.0	34.0	35.0	30.0	36.0
92-93	33.73418261136254	35.0	34.0	35.0	30.0	36.0
94-95	33.702825774748646	35.0	34.0	35.0	30.0	36.0
96-97	33.696279368087716	35.0	34.0	35.0	30.0	36.0
98-99	33.7251059856827	35.0	34.0	35.0	30.0	36.0
100	33.03802511655766	34.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	28.0
17	231.0
18	1094.0
19	3404.0
20	8669.0
21	16764.0
22	26875.0
23	38040.0
24	50355.0
25	63101.0
26	70796.0
27	73473.0
28	84942.0
29	104210.0
30	122641.0
31	147326.0
32	187925.0
33	247171.0
34	342291.0
35	495175.0
36	796217.0
37	1464772.0
38	2086034.0
39	1299635.0
40	58547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.292719619981064	20.30053209891964	14.448162879344654	23.958585401754647
2	14.233529175451231	20.67534010882609	42.13361644226415	22.957514273458525
3	18.112221837579753	25.826795838054334	30.11641398395664	25.94456834040927
4	12.451649343997559	17.827131630756803	38.93451300480974	30.7867060204359
5	13.559462609158077	37.89955832841924	35.40133619743892	13.139642864983767
6	30.298907687912667	37.46149623966027	19.070671276011826	13.168924796415238
7	26.88674206376312	32.585987562098346	23.026745237692932	17.5005251364456
8	24.344455239531104	37.055276092899824	21.114474876090437	17.48579379147864
9	25.192282585211483	17.92808124875557	21.401921792437122	35.47771437359582
10-11	24.04927319837198	27.107133556871776	28.905467166531135	19.938126078225103
12-13	24.60961992230527	24.590151307022566	28.892028458968916	21.90820031170325
14-15	21.94768889806339	26.156764108109886	26.200084111026555	25.695462882800168
16-17	21.96525669975042	29.191348110887045	26.890905390205972	21.952489799156563
18-19	22.04267563336243	28.812750454461455	27.920426351954237	21.22414756022188
20-21	22.907456511586197	27.86717097620318	27.703923870416652	21.52144864179397
22-23	22.68586787689112	27.74322233312348	27.46803171596118	22.10287807402421
24-25	22.359545049559475	27.907356696995965	27.545426599463873	22.187671653980683
26-27	22.33328015475032	28.00963804434013	27.454821747599254	22.202260053310297
28-29	22.2788761592574	28.062030068768877	27.50032748157743	22.158766290396294
30-31	22.567102558895286	27.86934052230378	27.438984792466503	22.124572126334424
32-33	22.297530352866005	27.96483773735411	27.460263553214304	22.277368356565585
34-35	22.45662103955974	27.943284815280716	27.489607240760332	22.110486904399217
36-37	22.447983247850622	27.833720198759533	27.490832470285696	22.22746408310415
38-39	22.30899769806266	27.952131888627253	27.573279301511512	22.165591111798573
40-41	22.48829457166585	27.78608228106854	27.55532444818305	22.17029869908256
42-43	22.329499493152316	27.863004164087858	27.5136095771293	22.29388676563053
44-45	22.372468634306696	27.866522918060948	27.48135083026885	22.27965761736351
46-47	22.344795674276813	27.815835981125826	27.452463932311318	22.386904412286047
48-49	22.289477940861214	27.871787413974715	27.52811910617339	22.31061553899069
50-51	22.210864629507682	27.918616280469184	27.564673613050406	22.30584547697273
52-53	22.3731839242799	27.8725119799014	27.4564982363972	22.2978058594215
54-55	22.404604198471787	27.740339115506885	27.505416769849887	22.349639916171434
56-57	22.31910939917415	27.835992973186336	27.524300452833778	22.32059717480574
58-59	22.31790944202575	27.869632068196186	27.534091404049715	22.27836708572834
60-61	22.377668188395567	27.82019248416048	27.534824318786583	22.26731500865737
62-63	22.36134451710653	27.895597371408375	27.50395125163678	22.239106859848313
64-65	22.3543367488196	27.87964450503026	27.487192383684345	22.2788263624658
66-67	22.384520450167717	27.851183610256918	27.413602634542872	22.350693305032497
68-69	22.328597754735274	27.803560270096867	27.492137946454527	22.375704028713333
70-71	22.304347216267097	27.684168170927236	27.536135056030282	22.475349556775388
72-73	22.363223968073815	27.739518573235195	27.486530636065126	22.410726822625865
74-75	22.217117429918638	27.814005211916825	27.545504596660646	22.42337276150389
76-77	22.340312329553075	27.807394079049104	27.45718933271769	22.395104258680135
78-79	22.244826115188307	27.791061693998977	27.45285271510978	22.51125947570294
80-81	22.30265756743371	27.81153369547273	27.504154127374676	22.381654609718886
82-83	22.30079189897798	27.800029112927177	27.607721250401028	22.29145773769381
84-85	22.366670105471506	27.822011258234824	27.51755499023195	22.293763646061716
86-87	22.206309136582746	27.87352923628299	27.59767681568211	22.322484811452153
88-89	22.25881120932015	27.800002631342725	27.574623130934782	22.366563028402346
90-91	22.258693870618064	27.91629554281128	27.53752360524831	22.287486981322346
92-93	22.30417764388215	27.868771192322185	27.594215449036202	22.232835714759464
94-95	22.155885533512382	27.9691728938612	27.61221741313508	22.262724159491338
96-97	22.209617627960696	28.096788179655768	27.674458553860877	22.01913563852266
98-99	23.288209675795294	30.021573805975255	27.855526791334967	18.834689726894485
100	22.239020106196982	31.0402366768115	21.58022647801593	25.140516738975588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3036.0
1	2248.0
2	1542.0
3	1586.5
4	1507.0
5	1508.5
6	1738.5
7	2093.5
8	2377.0
9	2714.5
10	3088.0
11	3386.5
12	3724.5
13	4179.5
14	4769.5
15	5567.0
16	6694.5
17	8066.5
18	9793.0
19	12187.5
20	15285.5
21	19436.0
22	25008.0
23	32157.0
24	41586.0
25	53205.5
26	67254.5
27	82991.5
28	99811.5
29	119558.0
30	139193.0
31	158020.0
32	177673.5
33	196124.5
34	213871.0
35	230468.0
36	246704.0
37	261620.5
38	274370.5
39	285442.5
40	295305.5
41	303826.5
42	309035.5
43	314132.5
44	319342.0
45	324237.5
46	327554.0
47	328264.0
48	326860.0
49	323410.5
50	319139.0
51	311597.5
52	299523.0
53	283905.0
54	267827.0
55	251858.5
56	234364.0
57	214781.5
58	192686.5
59	168655.0
60	143289.0
61	119138.5
62	97192.0
63	77635.0
64	61449.5
65	48591.0
66	38181.5
67	29785.0
68	23196.0
69	18205.5
70	14319.0
71	10904.5
72	8239.0
73	6213.5
74	4735.5
75	3590.0
76	2634.0
77	1921.5
78	1444.0
79	1096.0
80	829.5
81	613.5
82	472.0
83	340.0
84	245.0
85	190.5
86	151.5
87	121.5
88	83.5
89	53.5
90	39.0
91	29.0
92	21.0
93	11.0
94	4.5
95	3.0
96	2.5
97	4.0
98	3.5
99	3.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012323939367758803
3	0.02337697248821748
4	0.020026401472608056
5	0.011528018283591047
6	0.015302224714967179
7	0.016239357604390506
8	0.0021181770788335445
9	1.283743684141542E-4
10-11	7.06059026277848E-5
12-13	0.0032992212682437633
14-15	1.6688667893840047E-4
16-17	5.455910657601554E-4
18-19	3.209359210353855E-5
20-21	9.632224955348216E-5
22-23	1.285066575444074E-5
24-25	3.2148692589829235E-5
26-27	0.002509777352502798
28-29	0.003898054722502961
30-31	0.0027821584760071754
32-33	0.004213453148148508
34-35	0.006318808775312252
36-37	0.0012330653319115024
38-39	0.0013327449997278707
40-41	6.741075806966254E-4
42-43	5.322280376266595E-5
44-45	1.0053902321647125E-4
46-47	0.004595927102918826
48-49	9.542842626051237E-4
50-51	2.4056166612735033E-4
52-53	0.004580126753795321
54-55	2.445646040810006E-4
56-57	1.8332746685439399E-4
58-59	7.615190611423654E-4
60-61	7.90134751017047E-5
62-63	0.0
64-65	2.1944309730765262E-5
66-67	0.001972410920714769
68-69	0.0
70-71	3.6949701125932804E-4
72-73	0.001851319960657546
74-75	1.1545420762853671E-4
76-77	7.77852425682229E-6
78-79	5.505468463850819E-5
80-81	0.0031585089355570294
82-83	1.1265354376264286E-4
84-85	6.264660424081713E-4
86-87	0.013766037268851119
88-89	6.994659577412645E-4
90-91	8.602437104166657E-4
92-93	3.4188045876255E-5
94-95	1.9945482924802146E-4
96-97	6.195108024098617E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2303.0
20-21	4442.0
22-23	5133.0
24-25	6215.0
26-27	8606.0
28-29	12423.0
30-31	19858.0
32-33	24095.0
34-35	32288.0
36-37	45602.0
38-39	50777.0
40-41	49853.0
42-43	53172.0
44-45	61337.0
46-47	62760.0
48-49	61528.0
50-51	58748.0
52-53	59259.0
54-55	63737.0
56-57	65859.0
58-59	64842.0
60-61	63079.0
62-63	62134.0
64-65	66029.0
66-67	68700.0
68-69	68971.0
70-71	68453.0
72-73	66776.0
74-75	67297.0
76-77	70137.0
78-79	72619.0
80-81	71685.0
82-83	68419.0
84-85	68452.0
86-87	71142.0
88-89	74852.0
90-91	78198.0
92-93	81620.0
94-95	98551.0
96-97	259805.0
98-99	488201.0
100-101	4941760.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.13809530269239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.26272508221774	77.75059215412794
2	6.91533285960456	11.913472018209157
3	1.630747830874239	4.214085362115122
4	0.5396860988289869	1.8595013045787812
5	0.2535839212014679	1.0921617985841239
6	0.13865264826837057	0.7165965018306953
7	0.07401337747387	0.4462759953762852
8	0.050561895248301694	0.3484244281266368
9	0.031647593801989365	0.24534571059149912
>10	0.1015443917938627	1.3176674344221013
>50	0.0013539071738516274	0.07834625148651404
>100	1.5039351276397274E-4	0.01753104055116821
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.283743684141542E-5	0.0	0.0	0.0
3	0.0	1.283743684141542E-5	0.0	1.283743684141542E-5	0.0
4	0.0	3.8512310524246256E-5	0.0	1.283743684141542E-5	1.283743684141542E-5
5	1.283743684141542E-5	3.8512310524246256E-5	0.0	3.8512310524246256E-5	1.283743684141542E-5
6	2.567487368283084E-5	3.8512310524246256E-5	0.0	3.8512310524246256E-5	1.283743684141542E-5
7	2.567487368283084E-5	3.8512310524246256E-5	0.0	3.8512310524246256E-5	1.283743684141542E-5
8	2.567487368283084E-5	3.8512310524246256E-5	0.0	3.8512310524246256E-5	1.283743684141542E-5
9	2.567487368283084E-5	1.283743684141542E-4	0.0	5.134974736566168E-5	1.283743684141542E-5
10-11	2.567487368283084E-5	1.283743684141542E-4	0.0	9.628077631061564E-5	1.283743684141542E-5
12-13	2.567487368283084E-5	1.283743684141542E-4	0.0	1.1553693157273878E-4	1.283743684141542E-5
14-15	3.8512310524246256E-5	2.4391129998689297E-4	0.0	1.283743684141542E-4	2.567487368283084E-5
16-17	8.986205788990794E-5	2.567487368283084E-4	0.0	1.283743684141542E-4	3.8512310524246256E-5
18-19	9.628077631061565E-5	2.824236105111392E-4	0.0	1.283743684141542E-4	5.134974736566168E-5
20-21	1.0269949473132336E-4	3.0809848419397005E-4	0.0	1.6046796051769275E-4	5.134974736566168E-5
22-23	1.0269949473132336E-4	3.3377335787680094E-4	0.0	2.3749258156618525E-4	5.776846578636939E-5
24-25	1.0269949473132336E-4	3.7870438682175486E-4	0.0	4.300541341874166E-4	6.41871842070771E-5
26-27	1.0269949473132336E-4	4.0437926050458574E-4	0.0	8.85783142057664E-4	8.344333946920022E-5
28-29	1.0269949473132336E-4	4.1079797892529344E-4	0.0	0.0036394133445412716	1.0269949473132336E-4
30-31	1.0269949473132336E-4	4.3647285260812427E-4	0.0	0.013671870236107423	1.0269949473132336E-4
32-33	1.0269949473132336E-4	4.3647285260812427E-4	0.0	0.038608591300556876	1.0269949473132336E-4
34-35	1.0269949473132336E-4	5.134974736566168E-4	0.0	0.064431095507064	1.1553693157273878E-4
36-37	1.0269949473132336E-4	6.033595315465247E-4	0.0	0.097327027413191	1.1553693157273878E-4
38-39	1.0269949473132336E-4	6.41871842070771E-4	0.0	0.12926015155621187	1.1553693157273878E-4
40-41	1.0269949473132336E-4	7.06059026277848E-4	0.0	0.15350365103122487	1.1553693157273878E-4
42-43	1.0269949473132336E-4	7.188964631192635E-4	0.0	0.177503239206251	1.1553693157273878E-4
44-45	1.0269949473132336E-4	8.151772394298792E-4	0.0	0.19404427657641476	1.1553693157273878E-4
46-47	1.0269949473132336E-4	8.986205788990794E-4	0.0	0.20684961982572667	1.1553693157273878E-4
48-49	1.0269949473132336E-4	8.986205788990794E-4	0.0	0.21634290436995338	1.5404924209698503E-4
50-51	1.0911821315203107E-4	0.0010205762288925258	0.0	0.22388489851428495	1.5404924209698503E-4
52-53	1.1553693157273878E-4	0.0010269949473132335	0.0	0.22948843969556276	1.6046796051769275E-4
54-55	1.1553693157273878E-4	0.0010719259762581876	0.0	0.23400079874532026	1.6688667893840047E-4
56-57	1.1553693157273878E-4	0.001104019568361726	0.0	0.23845538932929142	1.6688667893840047E-4
58-59	1.1553693157273878E-4	0.0011296944420445569	0.0	0.24313463505798733	1.6688667893840047E-4
60-61	1.1553693157273878E-4	0.0011553693157273879	0.0	0.24670344249990084	1.6688667893840047E-4
62-63	1.1553693157273878E-4	0.0011810441894102186	0.0	0.2501759691655037	1.6688667893840047E-4
64-65	1.1553693157273878E-4	0.0013094185578243728	0.0	0.2530387175811394	1.6688667893840047E-4
66-67	1.1553693157273878E-4	0.0013350934315072038	0.0	0.25595281574414064	1.6688667893840047E-4
68-69	1.1553693157273878E-4	0.00137360574203145	0.0	0.2588476577518798	1.6688667893840047E-4
70-71	1.1553693157273878E-4	0.001392861897293573	0.0	0.26138305152805935	1.6688667893840047E-4
72-73	1.283743684141542E-4	0.0013992806157142807	0.0	0.2634755537332101	1.7972411577981589E-4
74-75	1.283743684141542E-4	0.0014570490815006502	0.0	0.2652407012989047	1.7972411577981589E-4
76-77	1.412118052555696E-4	0.0015276549841284348	0.0	0.2670892922040685	1.7972411577981589E-4
78-79	1.5404924209698503E-4	0.001572586013073389	0.0	0.2684693166645207	1.7972411577981589E-4
80-81	1.5404924209698503E-4	0.0015854234499148043	0.0	0.26916895697237786	1.7972411577981589E-4
82-83	1.5404924209698503E-4	0.001617517042018343	0.0	0.26959901110656526	1.925615526212313E-4
84-85	1.5404924209698503E-4	0.001617517042018343	0.0	0.26972738547497943	1.925615526212313E-4
86-87	1.6046796051769275E-4	0.001636773197280466	0.0	0.26976589778550364	1.925615526212313E-4
88	1.6688667893840047E-4	0.0017844037209567433	0.0	0.26976589778550364	1.925615526212313E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6170	0.0	34.810844	1
GTATCAA	10140	0.0	31.939402	1
TCAACGC	10415	0.0	29.10577	4
ATCAACG	10750	0.0	28.160149	3
CAACGCA	11030	0.0	27.403795	5
TATCAAC	11985	0.0	25.999525	2
AACGCAG	11660	0.0	25.956236	6
ACGCAGA	13955	0.0	21.626549	7
CGCAGAG	14500	0.0	20.637062	8
GTACATG	23125	0.0	20.367579	1
TACATGG	23845	0.0	19.362494	2
ACATGGG	23865	0.0	18.708237	3
CATGGGG	17935	0.0	16.83124	4
GCAGAGT	18105	0.0	16.293816	9
GTGGTAT	4520	0.0	15.247026	1
AGAGTAC	15345	0.0	14.91421	10-11
CAGAGTA	17655	0.0	14.267477	10-11
GTATAAG	3090	0.0	13.682879	1
TGGTATC	4560	0.0	13.444329	2
ATGGGGG	10800	0.0	13.347835	5
>>END_MODULE