FastQCFastQC Report
Fri 27 May 2016
SRR2140242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6954727
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58600.036.1536561
GTATCAA93950.030.7872871
TCAACGC102250.026.9639744
ATCAACG104700.026.252993
CAACGCA105450.026.0642075
TATCAAC111700.025.0999262
AACGCAG109500.024.9825446
ACGCAGA131400.020.8503657
GTACATG223700.020.4348681
CGCAGAG134950.020.206438
TACATGG224600.019.4304122
ACATGGG228750.018.6712063
CATGGGG171750.017.0622434
GCAGAGT166950.016.1052429
AGAGTAC145400.015.03220510-11
GGACCGT7600.014.4645966
CAGAGTA164400.014.42638810-11
TATACCG5900.014.3336145
GTACTAG16950.013.9710941
GTACTGG40900.013.33762551
Produced by FastQC (version 0.11.5)