##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6954727
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37430973207144	31.0	31.0	34.0	30.0	34.0
2	31.4863551078281	31.0	31.0	34.0	29.0	34.0
3	31.599900758146223	31.0	31.0	34.0	29.0	34.0
4	35.25539809111127	37.0	35.0	37.0	33.0	37.0
5	35.37966551382966	37.0	35.0	37.0	33.0	37.0
6	35.53406481663479	37.0	35.0	37.0	33.0	37.0
7	35.50616106139033	37.0	35.0	37.0	33.0	37.0
8	35.44692149670289	37.0	35.0	37.0	33.0	37.0
9	37.113960476090575	39.0	37.0	39.0	33.0	39.0
10-11	37.11566075850281	39.0	37.0	39.0	33.0	39.0
12-13	37.06573500296992	39.0	37.0	39.0	33.0	39.0
14-15	38.44818099977181	40.0	38.0	41.0	33.0	41.0
16-17	38.44825519391344	40.0	38.0	41.0	33.0	41.0
18-19	38.46021339155369	40.0	38.0	41.0	33.5	41.0
20-21	38.518394940336805	40.0	38.0	41.0	34.0	41.0
22-23	38.49988001922152	40.0	38.0	41.0	33.0	41.0
24-25	38.527887769124035	40.0	38.0	41.0	33.0	41.0
26-27	38.51495418568062	40.0	38.0	41.0	33.0	41.0
28-29	38.45616102376347	40.0	38.0	41.0	33.0	41.0
30-31	38.39025175218501	40.0	38.0	41.0	33.0	41.0
32-33	38.35683004423086	40.0	38.0	41.0	33.0	41.0
34-35	38.2886025881341	40.0	38.0	41.0	33.0	41.0
36-37	38.20435917716047	40.0	38.0	41.0	33.0	41.0
38-39	38.11607667200987	40.0	38.0	41.0	33.0	41.0
40-41	38.0655219461231	40.0	38.0	41.0	33.0	41.0
42-43	37.91068428631173	40.0	37.0	41.0	32.0	41.0
44-45	37.72064204443233	40.0	37.0	41.0	32.0	41.0
46-47	37.56808903343374	40.0	37.0	41.0	31.0	41.0
48-49	37.431478465207285	40.0	36.0	41.0	31.0	41.0
50-51	37.295062583706944	39.0	36.0	41.0	31.0	41.0
52-53	37.143104128812524	39.0	36.0	40.5	31.0	41.0
54-55	36.962000584650525	39.0	35.0	40.0	30.5	41.0
56-57	36.92122005722361	39.0	35.0	40.0	30.5	41.0
58-59	36.96158386743733	39.0	35.0	40.0	31.0	41.0
60-61	37.23802095651207	39.0	35.0	41.0	31.0	41.0
62-63	37.174098297704006	39.0	35.0	41.0	31.0	41.0
64-65	36.93923638337438	38.5	35.0	41.0	31.0	41.0
66-67	36.70577235063952	38.0	35.0	40.0	31.0	41.0
68-69	36.406926195164075	37.0	35.0	40.0	31.0	41.0
70-71	36.14909651597259	37.0	35.0	39.5	31.0	41.0
72-73	35.84126715289699	36.0	35.0	39.0	31.0	41.0
74-75	35.53172395943065	36.0	34.0	39.0	30.0	41.0
76-77	35.23297719169378	35.5	34.0	38.5	30.0	40.0
78-79	34.93502016365851	35.0	34.0	37.0	30.0	39.0
80-81	34.648182725457566	35.0	34.0	37.0	30.0	39.0
82-83	34.40919170192349	35.0	34.0	36.0	30.0	39.0
84-85	34.19250001324967	35.0	34.0	36.0	30.0	37.0
86-87	34.00964516086236	35.0	34.0	36.0	30.0	37.0
88-89	33.86630860063708	35.0	34.0	35.0	30.0	37.0
90-91	33.74857990136357	35.0	34.0	35.0	30.0	36.0
92-93	33.68003556378746	35.0	34.0	35.0	30.0	36.0
94-95	33.65311576074692	35.0	34.0	35.0	30.0	36.0
96-97	33.651639691819454	35.0	34.0	35.0	30.0	36.0
98-99	33.68297914702575	35.0	34.0	35.0	30.0	35.5
100	32.994327987614625	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	24.0
17	221.0
18	990.0
19	3305.0
20	8265.0
21	16412.0
22	25800.0
23	36465.0
24	48258.0
25	60169.0
26	66066.0
27	69533.0
28	79459.0
29	97018.0
30	113132.0
31	134837.0
32	172079.0
33	224668.0
34	311994.0
35	449409.0
36	726091.0
37	1333673.0
38	1825155.0
39	1102394.0
40	49307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40789422791146	20.204272576047916	14.354725929572792	24.03310726646783
2	14.274320850276515	20.676459857872132	41.97974971954502	23.069469572306332
3	18.229877525942932	25.81483259520223	29.990482006274572	25.96480787258027
4	12.54294507158339	17.766693766279	38.79020197288821	30.9001591892494
5	13.644432301066301	37.88363448112224	35.1999223462924	13.272010871519067
6	30.28521868481936	37.3689484468241	19.023744303794558	13.322088564561978
7	27.008215558018854	32.501082886708474	22.84189163769861	17.648809917574056
8	24.418710487375154	36.902726418744706	21.06486357697178	17.61369951690835
9	25.277552511100374	17.86694503876504	21.30748613890998	35.54801631122461
10-11	24.22140276264217	27.00892731970911	28.670288476069274	20.09938144157945
12-13	24.761660247935318	24.451422963972114	28.76211146556507	22.0248053225275
14-15	22.10828912471611	26.052613227141585	26.03015364396672	25.808944004175583
16-17	22.124192451424864	29.067205008418778	26.670548938917477	22.138053601238877
18-19	22.177197673322645	28.6494885598991	27.78591732857283	21.387396438205425
20-21	23.105666966460493	27.747155842728503	27.461841021807842	21.68533616900316
22-23	22.895632322427677	27.577487704622765	27.274847213298536	22.252032759651026
24-25	22.53645387071365	27.772747151942312	27.323195990045413	22.36760298729862
26-27	22.49922498504041	27.860720799959626	27.283814082922998	22.35624013207697
28-29	22.454001449616722	27.90493424787724	27.305713137149752	22.335351165356283
30-31	22.683150098381635	27.748430261855404	27.255473472828328	22.31294616693463
32-33	22.44473434210081	27.83771044879333	27.283123487822138	22.43443172128373
34-35	22.580345533582324	27.833205462457606	27.264856515493907	22.321592488466163
36-37	22.611254010742847	27.675447810889004	27.3130820708801	22.400216107488056
38-39	22.46314860938606	27.72352414767863	27.478614898871324	22.334712344063988
40-41	22.605656951242192	27.5791181539424	27.460006929112662	22.355217965702742
42-43	22.47610032282983	27.72942276121458	27.334212966477196	22.460263949478396
44-45	22.53921477726993	27.742894463673135	27.313263920156285	22.40462683890065
46-47	22.517824193567034	27.694890280712674	27.25590431253026	22.531381213190027
48-49	22.45078416105233	27.684320382960408	27.35951020859733	22.505385247389935
50-51	22.352007204733724	27.76476163322561	27.403695191883582	22.479535970157087
52-53	22.49985671332907	27.69728251019659	27.25987864780756	22.542982128666782
54-55	22.5298545201077	27.627394970781015	27.28390544490371	22.55884506420757
56-57	22.494127753139058	27.69806740917769	27.311888122910332	22.495916714772925
58-59	22.504988396534273	27.712655632602655	27.319358796659955	22.46299717420312
60-61	22.542486480132993	27.649543080761767	27.345088152310804	22.46288228679444
62-63	22.511757547925676	27.760014849507357	27.29057985592381	22.437647746643165
64-65	22.53992978686261	27.74270119023819	27.25130734866706	22.466061674232137
66-67	22.5436394274194	27.707487634401414	27.18131900709227	22.567553931086923
68-69	22.480762764822245	27.665042253920657	27.304033833171893	22.550161148085206
70-71	22.455347389983032	27.528830594996297	27.359885369859523	22.655936645161148
72-73	22.55819888401635	27.58317580299996	27.302582219330795	22.556043093652903
74-75	22.38270390049778	27.779843747268952	27.31766546844263	22.519786883790637
76-77	22.527293045346724	27.641323310035016	27.24673188036764	22.58465176425062
78-79	22.418728729087373	27.637483127859948	27.27926073419349	22.664527408859193
80-81	22.435955905695852	27.69031693271019	27.304051740509315	22.569675421084643
82-83	22.462494236346167	27.68729993914879	27.388369390355233	22.46183643414981
84-85	22.524823623183625	27.719307007300042	27.329124507168352	22.42674486234798
86-87	22.384339051448283	27.69904743569956	27.42705480291967	22.48955870993249
88-89	22.411867116232937	27.676320123459337	27.38647473410621	22.525338026201517
90-91	22.456491656430096	27.75788550026914	27.357548426057416	22.428074417243344
92-93	22.459179711232657	27.738898878532115	27.37458568322068	22.427335727014547
94-95	22.31269360503144	27.803953213685496	27.446670669320323	22.43668251196274
96-97	22.380954319127095	27.978247836830384	27.434731647443733	22.20606619659879
98-99	23.462401338236983	29.887493885537257	27.572484960794107	19.077619815431653
100	22.472395850489495	30.782502814084168	21.364595129675042	25.380506205751296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2729.0
1	1990.0
2	1364.0
3	1435.5
4	1360.0
5	1414.0
6	1640.0
7	1942.5
8	2173.5
9	2431.5
10	2785.5
11	3057.5
12	3285.0
13	3658.0
14	4229.5
15	4985.0
16	5972.5
17	7155.0
18	8626.5
19	10602.5
20	13301.0
21	16929.0
22	21950.0
23	28549.0
24	36986.5
25	47823.5
26	60583.0
27	73823.5
28	88936.5
29	105737.5
30	120963.5
31	136317.0
32	152275.5
33	166242.5
34	179875.0
35	192748.5
36	207363.0
37	223254.5
38	234633.0
39	243148.5
40	251825.5
41	259618.0
42	265980.0
43	273437.5
44	280398.5
45	287521.5
46	291719.0
47	293255.5
48	294568.5
49	293453.0
50	291151.5
51	285816.5
52	277494.0
53	267294.5
54	255927.5
55	241230.5
56	222925.0
57	203117.5
58	181774.5
59	159129.5
60	134420.0
61	110698.0
62	90481.0
63	71504.5
64	54617.5
65	41786.0
66	32194.5
67	25190.0
68	19656.5
69	15308.0
70	12077.5
71	9394.0
72	7171.5
73	5387.5
74	4181.5
75	3244.0
76	2409.5
77	1828.0
78	1385.0
79	1012.5
80	760.0
81	559.0
82	427.5
83	301.0
84	199.5
85	140.0
86	108.0
87	86.0
88	57.0
89	37.5
90	30.0
91	25.0
92	20.0
93	11.0
94	4.5
95	3.0
96	2.5
97	2.0
98	2.0
99	6.0
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012193145755397731
3	0.022732739904815818
4	0.01967007475634917
5	0.011172257372575517
6	0.014019241876783948
7	0.01568717219238081
8	0.0020992916041132886
9	1.0065096732050016E-4
10-11	1.1502967693771445E-4
12-13	0.003041097084040826
14-15	1.7254451540657168E-4
16-17	5.320122558369293E-4
18-19	3.594677404303577E-5
20-21	5.7541968234531854E-5
22-23	7.1973285819448965E-6
24-25	2.881044828193213E-5
26-27	0.00256649529783457
28-29	0.003796780431249293
30-31	0.0027769405173555892
32-33	0.004291970267375973
34-35	0.0062040438001128365
36-37	0.0011614619899145654
38-39	0.0014108831114205257
40-41	5.768064783945671E-4
42-43	5.953520271885364E-5
44-45	6.745882313435879E-5
46-47	0.004020091692850788
48-49	7.924180828282621E-4
50-51	3.1499866932269447E-4
52-53	0.004592707755937159
54-55	2.342980294364234E-4
56-57	1.7337924100400138E-4
58-59	6.443014006953364E-4
60-61	1.6057323359808648E-5
62-63	0.0
64-65	1.6353354117901304E-5
66-67	0.0020396277373847995
68-69	0.0
70-71	3.6256018077419243E-4
72-73	0.001959771013538183
74-75	1.635935193546632E-4
76-77	2.6111701332271227E-5
78-79	8.80304627655791E-6
80-81	0.0034649006896221237
82-83	1.4417561599257205E-4
84-85	4.192161387277337E-4
86-87	0.013975701822685956
88-89	6.811940790983901E-4
90-91	0.0010493281605551495
92-93	2.874869852246064E-5
94-95	2.236657632190599E-4
96-97	8.934504719701754E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2232.0
20-21	4234.0
22-23	4947.0
24-25	5940.0
26-27	8016.0
28-29	11039.0
30-31	17079.0
32-33	20422.0
34-35	27203.0
36-37	38634.0
38-39	43037.0
40-41	42440.0
42-43	45789.0
44-45	53075.0
46-47	54828.0
48-49	54534.0
50-51	52414.0
52-53	52897.0
54-55	56863.0
56-57	58656.0
58-59	58138.0
60-61	57180.0
62-63	55857.0
64-65	58551.0
66-67	62276.0
68-69	62563.0
70-71	62638.0
72-73	61024.0
74-75	61793.0
76-77	63893.0
78-79	66516.0
80-81	65606.0
82-83	62537.0
84-85	62278.0
86-87	64452.0
88-89	68340.0
90-91	71122.0
92-93	73692.0
94-95	88945.0
96-97	234747.0
98-99	436335.0
100-101	4401965.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.78304620779271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.93950053431881	77.15284330241268
2	7.103065263237121	12.186451513864744
3	1.6761859814072708	4.313650184877429
4	0.6201039201195265	2.12777612932987
5	0.27141094629313195	1.1641228873582241
6	0.13955162827031517	0.7182698265771096
7	0.07833490591024586	0.47038647973672004
8	0.04380448218944152	0.3006145535812235
9	0.03205715681152196	0.24749645076479207
>10	0.09448795763028169	1.223643486732409
>50	0.0013322636594711688	0.07379659320993359
>100	1.649601529012727E-4	0.020948591554939274
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.751483846885722E-5	0.0	0.0	0.0
3	0.0	5.751483846885722E-5	0.0	0.0	0.0
4	0.0	1.1502967693771445E-4	0.0	0.0	1.4378709617214306E-5
5	0.0	1.1502967693771445E-4	0.0	1.4378709617214306E-5	1.4378709617214306E-5
6	0.0	1.1502967693771445E-4	0.0	2.875741923442861E-5	1.4378709617214306E-5
7	0.0	1.1502967693771445E-4	0.0	2.875741923442861E-5	1.4378709617214306E-5
8	0.0	1.4378709617214307E-4	0.0	2.875741923442861E-5	1.4378709617214306E-5
9	0.0	2.731954827270718E-4	0.0	2.875741923442861E-5	2.875741923442861E-5
10-11	0.0	2.731954827270718E-4	0.0	2.875741923442861E-5	2.875741923442861E-5
12-13	0.0	2.731954827270718E-4	0.0	3.594677404303577E-5	2.875741923442861E-5
14-15	0.0	3.666570952389648E-4	0.0	6.470419327746438E-5	5.751483846885723E-5
16-17	0.0	3.9541451447339343E-4	0.0	1.1502967693771445E-4	7.189354808607153E-5
18-19	0.0	4.6011870775085784E-4	0.0	1.5097645098075023E-4	8.627225770328584E-5
20-21	0.0	5.176335462197151E-4	0.0	1.7973387021517884E-4	8.627225770328584E-5
22-23	0.0	5.895270943057866E-4	0.0	3.1633161157871474E-4	9.346161251189299E-5
24-25	0.0	6.039058039230009E-4	0.0	5.967164491143936E-4	1.0065096732050014E-4
26-27	0.0	6.182845135402152E-4	0.0	0.0011215393501427158	1.078403221291073E-4
28-29	0.0	6.182845135402152E-4	0.0	0.005593318041096365	1.1502967693771445E-4
30-31	0.0	7.405035452865368E-4	0.0	0.018958328630297065	1.1502967693771445E-4
32-33	0.0	7.476929000951439E-4	0.0	0.0527626749403679	1.1502967693771445E-4
34-35	0.0	8.986693510758941E-4	0.0	0.08905453801421681	1.1502967693771445E-4
36-37	0.0	0.0010568351568652516	0.0	0.13735981297324829	1.1502967693771445E-4
38-39	0.0	0.0011359180597599302	0.0	0.18269588439632498	1.2940838655492876E-4
40-41	0.0	0.0012797051559320731	0.0	0.21885833908361896	1.4378709617214307E-4
42-43	0.0	0.0013012732203578946	0.0	0.2554665337690466	1.5097645098075023E-4
44-45	0.0	0.0014450603165300376	0.0	0.2812116133386688	1.5816580578935737E-4
46-47	0.0	0.0015672793482763593	0.0	0.3023483164759738	1.653551605979645E-4
48-49	0.0	0.0015672793482763593	0.0	0.31705773641438406	1.7254451540657168E-4
50-51	0.0	0.0017973387021517884	0.0	0.3274823008868644	1.7254451540657168E-4
52-53	0.0	0.0018117174117690026	0.0	0.33535464440228924	1.7254451540657168E-4
54-55	7.189354808607153E-6	0.00181890676657761	0.0	0.34117083244245244	1.7254451540657168E-4
56-57	1.4378709617214306E-5	0.0018404748310034311	0.0	0.3473392988682374	1.7254451540657168E-4
58-59	1.4378709617214306E-5	0.0018404748310034311	0.0	0.35299732110261123	1.7254451540657168E-4
60-61	1.4378709617214306E-5	0.001883610959855074	0.0	0.35825273946770303	1.7254451540657168E-4
62-63	1.4378709617214306E-5	0.001926747088706717	0.0	0.36234348235380054	1.7254451540657168E-4
64-65	1.4378709617214306E-5	0.002113670313730503	0.0	0.3654996091147791	1.7254451540657168E-4
66-67	1.4378709617214306E-5	0.002135238378156324	0.0	0.36854070619881985	1.7254451540657168E-4
68-69	1.4378709617214306E-5	0.0021855638618165744	0.0	0.37133017586455946	1.7254451540657168E-4
70-71	1.4378709617214306E-5	0.002214321281051003	0.0	0.3736092013388879	1.7254451540657168E-4
72-73	1.4378709617214306E-5	0.002214321281051003	0.0	0.376096718102666	1.7254451540657168E-4
74-75	1.4378709617214306E-5	0.0022574574099026457	0.0	0.37828947131929114	1.7254451540657168E-4
76-77	1.4378709617214306E-5	0.0023149722483715033	0.0	0.3800364845377827	1.7254451540657168E-4
78-79	1.4378709617214306E-5	0.0023293509579887174	0.0	0.38137370453218367	1.7254451540657168E-4
80-81	1.4378709617214306E-5	0.0023293509579887174	0.0	0.38199198904572385	1.8692322502378598E-4
82-83	1.4378709617214306E-5	0.0023293509579887174	0.0	0.3824233503342403	2.156806442582146E-4
84-85	1.4378709617214306E-5	0.0023365403127973247	0.0	0.3826390309784985	2.156806442582146E-4
86-87	1.4378709617214306E-5	0.002358108377223146	0.0	0.38265340968811573	2.156806442582146E-4
88	1.4378709617214306E-5	0.002444380634926432	0.0	0.3826605990429243	2.300593538754289E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5860	0.0	36.153656	1
GTATCAA	9395	0.0	30.787287	1
TCAACGC	10225	0.0	26.963974	4
ATCAACG	10470	0.0	26.25299	3
CAACGCA	10545	0.0	26.064207	5
TATCAAC	11170	0.0	25.099926	2
AACGCAG	10950	0.0	24.982544	6
ACGCAGA	13140	0.0	20.850365	7
GTACATG	22370	0.0	20.434868	1
CGCAGAG	13495	0.0	20.20643	8
TACATGG	22460	0.0	19.430412	2
ACATGGG	22875	0.0	18.671206	3
CATGGGG	17175	0.0	17.062243	4
GCAGAGT	16695	0.0	16.105242	9
AGAGTAC	14540	0.0	15.032205	10-11
GGACCGT	760	0.0	14.464596	6
CAGAGTA	16440	0.0	14.426388	10-11
TATACCG	590	0.0	14.333614	5
GTACTAG	1695	0.0	13.971094	1
GTACTGG	4090	0.0	13.3376255	1
>>END_MODULE