FastQCFastQC Report
Fri 27 May 2016
SRR2140241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11797576
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93600.040.8124541
GTATCAA161350.031.8467241
TCAACGC174900.027.7591674
ATCAACG180350.026.9683133
CAACGCA184050.026.3313125
TATCAAC194850.025.6340482
AACGCAG190100.025.5572786
GTACATG387250.021.104021
ACGCAGA232000.020.812847
TACATGG389850.020.3237532
CGCAGAG243050.019.8645028
ACATGGG401300.019.1328813
CATGGGG302150.017.0753364
GCAGAGT297650.016.1212129
AGAGTAC252800.015.03805210-11
CAGAGTA285400.014.75595610-11
GTGGTAT69050.013.76952651
ATGGGGG180050.013.4464115
GTATAAG46750.013.0161041
GTATTAG37000.012.90556051
Produced by FastQC (version 0.11.5)