##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11797576
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.479861795338298	31.0	31.0	34.0	28.0	34.0
2	31.6203561646901	33.0	31.0	34.0	28.0	34.0
3	31.651723201444092	33.0	31.0	34.0	28.0	34.0
4	35.25819337802952	37.0	35.0	37.0	32.0	37.0
5	35.35604330923573	37.0	35.0	37.0	33.0	37.0
6	35.32893223150247	37.0	35.0	37.0	32.0	37.0
7	35.30733694786115	37.0	35.0	37.0	32.0	37.0
8	35.28401410594854	37.0	35.0	37.0	32.0	37.0
9	36.912468798675256	39.0	37.0	39.0	33.0	39.0
10-11	36.92174803535913	39.0	37.0	39.0	33.0	39.0
12-13	36.879520632034925	39.0	37.0	39.0	33.0	39.0
14-15	38.22853508212195	40.0	38.0	41.0	33.0	41.0
16-17	38.21771620712593	40.0	38.0	41.0	33.0	41.0
18-19	38.20802862384612	40.0	38.0	41.0	33.0	41.0
20-21	38.27701363729099	40.0	38.0	41.0	33.0	41.0
22-23	38.24277193556031	40.0	38.0	41.0	33.0	41.0
24-25	38.26947705518077	40.0	38.0	41.0	33.0	41.0
26-27	38.26190014681055	40.0	38.0	41.0	33.0	41.0
28-29	38.202481311572356	40.0	38.0	41.0	33.0	41.0
30-31	38.133403666902645	40.0	38.0	41.0	32.0	41.0
32-33	38.10521285669826	40.0	38.0	41.0	32.0	41.0
34-35	38.03121122611919	40.0	38.0	41.0	32.0	41.0
36-37	37.93653698606157	40.0	37.5	41.0	32.0	41.0
38-39	37.84247911424608	40.0	37.0	41.0	32.0	41.0
40-41	37.791590786129596	40.0	37.0	41.0	32.0	41.0
42-43	37.60876088734324	40.0	37.0	41.0	31.0	41.0
44-45	37.371492449809935	40.0	36.0	41.0	31.0	41.0
46-47	37.206848289601425	39.0	36.0	41.0	31.0	41.0
48-49	37.05760832137035	39.0	36.0	40.0	30.0	41.0
50-51	36.9112647414395	39.0	35.0	40.0	30.0	41.0
52-53	36.75238131586316	39.0	35.0	40.0	30.0	41.0
54-55	36.5651258902792	38.5	35.0	40.0	30.0	41.0
56-57	36.5283069229305	38.5	35.0	40.0	30.0	41.0
58-59	36.57728117847023	38.5	35.0	40.0	30.0	41.0
60-61	36.88182672327886	39.0	35.0	40.0	31.0	41.0
62-63	36.83225258823417	38.5	35.0	40.0	31.0	41.0
64-65	36.60350775872355	38.0	35.0	40.0	31.0	41.0
66-67	36.37378675427621	37.5	35.0	40.0	30.5	41.0
68-69	36.08055237823814	37.0	34.5	40.0	30.0	41.0
70-71	35.82820559339288	36.5	34.0	39.0	30.0	41.0
72-73	35.52983506357102	36.0	34.0	39.0	30.0	41.0
74-75	35.22769083031579	35.5	34.0	39.0	30.0	40.0
76-77	34.936099685206635	35.0	34.0	37.5	30.0	39.5
78-79	34.64666520448733	35.0	34.0	37.0	30.0	39.0
80-81	34.36680763743617	35.0	34.0	37.0	30.0	39.0
82-83	34.140137829097476	35.0	34.0	36.0	29.0	38.5
84-85	33.93117096117561	35.0	34.0	36.0	29.0	37.0
86-87	33.753753578942636	35.0	33.0	35.5	29.0	37.0
88-89	33.622414880766996	35.0	33.0	35.0	29.0	36.5
90-91	33.510928582048535	35.0	33.0	35.0	29.0	36.0
92-93	33.44870204997049	35.0	33.0	35.0	29.0	36.0
94-95	33.432548159369816	35.0	33.0	35.0	29.0	36.0
96-97	33.44549122974316	35.0	33.0	35.0	29.5	35.5
98-99	33.4916044282499	35.0	33.0	35.0	30.0	35.0
100	32.76593209238664	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	3.0
16	57.0
17	434.0
18	2032.0
19	6548.0
20	16431.0
21	32476.0
22	51513.0
23	72063.0
24	95253.0
25	118678.0
26	132935.0
27	138781.0
28	155534.0
29	186044.0
30	217638.0
31	257784.0
32	325120.0
33	422378.0
34	578889.0
35	826296.0
36	1316174.0
37	2292467.0
38	2887980.0
39	1599869.0
40	64197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49147248553432	20.004939997843625	14.324849443648425	24.178738072973633
2	14.401067131904979	20.446198130654146	41.85690954768545	23.29582518975543
3	18.383968606111097	25.474766354664773	29.738225150906235	26.4030398883179
4	12.789460289060553	17.493955650523592	38.17522789134736	31.541356169068496
5	13.880779921242889	37.617337958072845	34.99789933562039	13.503982785063886
6	30.85939549832904	36.88933073616656	18.726516796342736	13.524756969161656
7	27.43172955799159	32.295947420478065	22.443145089880094	17.82917793165025
8	24.758072992601736	36.83015522116813	20.69703730326107	17.71473448296907
9	25.504250668888595	17.74793166889241	21.032129114888637	35.71568854733036
10-11	24.500227419791507	26.877539824740122	28.302520197810292	20.31971255765808
12-13	24.98520416639824	24.29138086535495	28.411891255920878	22.311523712325933
14-15	22.3008698708813	25.8165812400713	25.749821647374322	26.132727241673077
16-17	22.352742979408184	28.91853822760744	26.389936365903786	22.338782427080588
18-19	22.469573933995836	28.449532439869945	27.466440450657075	21.614453175477145
20-21	23.32686561796777	27.511599310487316	27.262717936591567	21.898817134953347
22-23	23.140800433860377	27.36627897624671	27.032632943484025	22.460287646408887
24-25	22.79370092187071	27.53822648482018	27.07401792763567	22.59405466567345
26-27	22.73563816141792	27.628022188516653	27.06611338272129	22.570226267344136
28-29	22.69086550259203	27.635753793281964	27.1060413731119	22.56733933101411
30-31	22.943474392653812	27.449427410846173	27.05448816647944	22.55261003002057
32-33	22.68509663071979	27.585119942549113	27.077427744773246	22.652355681957857
34-35	22.83277658556787	27.589585367070207	27.07163867560794	22.50599937175398
36-37	22.839259558071404	27.428531662995166	27.081630597556135	22.6505781813773
38-39	22.68335709239416	27.53961021952817	27.228950847244555	22.54808184083312
40-41	22.851132206941337	27.379759130768395	27.176031608263333	22.593077054026935
42-43	22.695421423758262	27.47916969333749	27.099617481540932	22.725791401363313
44-45	22.722611068487446	27.499805670318768	27.115117007715995	22.662466253477792
46-47	22.745179112973123	27.411572717553785	27.083518837467853	22.759729332005247
48-49	22.688227038760008	27.45404934558354	27.13056995257064	22.727153663085815
50-51	22.570289501563668	27.530194904938405	27.163671105353888	22.73584448814404
52-53	22.76988354606921	27.47646544326094	27.06844542547518	22.685205585194673
54-55	22.77813756499701	27.345203112548045	27.12271280834708	22.753946514107863
56-57	22.715475497478568	27.469400394246087	27.110817938881382	22.704306169393966
58-59	22.667803570627182	27.451780461620917	27.18873553425597	22.691680433495932
60-61	22.728337989101732	27.414214137203786	27.17568258845387	22.68176528524062
62-63	22.744833128407592	27.489053154990017	27.149554242230938	22.616559474371453
64-65	22.71874633707164	27.46754905171405	27.129160905430954	22.68454370578335
66-67	22.7584345407956	27.43739718943964	27.025559581275658	22.7786086884891
68-69	22.694714385764488	27.4195593165797	27.13243150740152	22.75329479025429
70-71	22.624985590992093	27.26037277878882	27.185847122332362	22.928794507886717
72-73	22.689095989004908	27.36164202278932	27.101658506158294	22.847603482047475
74-75	22.554005651550675	27.485667566523375	27.16369161635082	22.796635165575125
76-77	22.69187280664037	27.403869694327597	27.07210122669726	22.832156272334775
78-79	22.56295358633987	27.404604476768828	27.10987543586213	22.922566501029173
80-81	22.602898361270686	27.4578347865767	27.11633877713474	22.82292807501787
82-83	22.628821981160876	27.41872696577567	27.232539502355625	22.71991155070783
84-85	22.691042338472617	27.431207142423364	27.183994755768076	22.693755763335947
86-87	22.513443847666466	27.52964750294612	27.237361759024886	22.71954689036253
88-89	22.59560310112404	27.44099360384894	27.207129849918214	22.756273445108803
90-91	22.60310135198431	27.530401257926034	27.19424967900126	22.672247711088392
92-93	22.615348972125187	27.518815805860804	27.2123526717115	22.65348255030251
94-95	22.457694786245735	27.60959725291433	27.237974102610657	22.694733858229277
96-97	22.504714518304333	27.754171440505587	27.286264139510326	22.454849901679754
98-99	23.61362858908274	29.676543588803916	27.44672769052477	19.263100131588576
100	22.634759104269857	30.65540100035508	21.11624964495404	25.593590250421023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5422.0
1	3957.0
2	2641.5
3	2715.5
4	2574.0
5	2581.5
6	2911.5
7	3407.5
8	3801.5
9	4306.0
10	4874.0
11	5271.5
12	5697.5
13	6358.0
14	7258.5
15	8385.0
16	9771.5
17	11501.0
18	13781.0
19	16886.0
20	20981.0
21	26780.5
22	34604.0
23	44304.0
24	56358.5
25	72536.0
26	93024.0
27	115106.0
28	138694.0
29	164928.5
30	190210.0
31	214489.0
32	239935.0
33	265403.0
34	291908.0
35	315635.0
36	339797.5
37	363569.5
38	382458.5
39	400057.5
40	418691.0
41	434983.5
42	446320.0
43	457672.5
44	470475.0
45	485229.5
46	495632.5
47	503667.5
48	509481.5
49	510181.0
50	508967.0
51	502485.0
52	488253.5
53	467740.5
54	447192.0
55	423356.5
56	393793.0
57	359520.5
58	322859.0
59	285888.0
60	244561.5
61	204052.0
62	167656.0
63	134480.0
64	106710.5
65	84680.0
66	66422.5
67	51181.0
68	39212.5
69	30338.5
70	23348.5
71	17480.0
72	13273.0
73	10108.0
74	7675.5
75	5772.5
76	4248.0
77	3170.0
78	2431.0
79	1860.5
80	1412.0
81	1066.0
82	829.0
83	574.0
84	395.0
85	283.5
86	219.5
87	178.0
88	117.0
89	72.5
90	50.5
91	37.0
92	28.5
93	16.0
94	7.5
95	4.5
96	4.0
97	4.5
98	3.0
99	6.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01246866305417316
3	0.022623291428679925
4	0.019173430202950165
5	0.011087023300379672
6	0.013943542300553943
7	0.015299753101823629
8	0.0018308845817140741
9	1.7800266766664609E-4
10-11	8.476317507935528E-5
12-13	0.0028480426826663374
14-15	1.7376450891267833E-4
16-17	4.111013991348732E-4
18-19	2.119079376983882E-5
20-21	9.753724809680444E-5
22-23	1.2732635867835238E-5
24-25	3.398522229075437E-5
26-27	0.0022412226681938732
28-29	0.0035006019161494266
30-31	0.002513136149854938
32-33	0.0038459402742159698
34-35	0.005695784256912405
36-37	0.0010908312785391967
38-39	0.0013404147139014929
40-41	5.763245105182061E-4
42-43	6.591656255145611E-5
44-45	7.525289510060714E-5
46-47	0.004155119955769173
48-49	6.706010092770223E-4
50-51	3.040778105163178E-4
52-53	0.004204894295637653
54-55	2.3531197638759665E-4
56-57	1.8623271836030658E-4
58-59	6.390932094419819E-4
60-61	3.320183509388436E-5
62-63	0.0
64-65	4.831562316113758E-6
66-67	0.0017177498803919045
68-69	0.0
70-71	3.388877534487934E-4
72-73	0.0015815241712548915
74-75	9.668938103783838E-5
76-77	5.143298202720733E-6
78-79	5.201528812542467E-6
80-81	0.0030206467520443175
82-83	1.6501706382902613E-4
84-85	3.9839809509719455E-4
86-87	0.01331786608893818
88-89	5.845732444355521E-4
90-91	7.88097954651055E-4
92-93	2.267493356953056E-5
94-95	2.130036911236929E-4
96-97	5.883032950573405E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4934.0
20-21	9240.0
22-23	10614.0
24-25	12239.0
26-27	15276.0
28-29	19695.0
30-31	29623.0
32-33	36026.0
34-35	47609.0
36-37	67027.0
38-39	75109.0
40-41	73281.0
42-43	79343.0
44-45	90919.0
46-47	94075.0
48-49	92979.0
50-51	89608.0
52-53	89751.0
54-55	96108.0
56-57	99436.0
58-59	98637.0
60-61	97040.0
62-63	95702.0
64-65	100914.0
66-67	105832.0
68-69	106607.0
70-71	106980.0
72-73	102413.0
74-75	102509.0
76-77	107748.0
78-79	111470.0
80-81	109192.0
82-83	105924.0
84-85	107105.0
86-87	110857.0
88-89	119332.0
90-91	124379.0
92-93	130460.0
94-95	158485.0
96-97	408726.0
98-99	750401.0
100-101	7403971.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.39104100780156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03287682538932	74.24544282388587
2	7.386672155540894	12.319645612677931
3	1.8743390956840391	4.689092651721402
4	0.7726669890895811	2.577340182901753
5	0.3543836287039387	1.4776209856871836
6	0.1860887238173834	0.9310879439366928
7	0.11675831514494459	0.6815618212377724
8	0.06904536660897073	0.46062119986298966
9	0.048206395664114214	0.361798336589801
>10	0.15538458238154432	1.9770010366924218
>50	0.002770500833199044	0.158491763070567
>100	7.891777904981228E-4	0.10912906428198994
>500	1.8243351561062825E-5	0.011166577453608746
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6952635015871056E-5	0.0	1.6952635015871056E-5	1.6952635015871056E-5
3	0.0	1.6952635015871056E-5	0.0	1.6952635015871056E-5	1.6952635015871056E-5
4	0.0	5.085790504761317E-5	0.0	5.085790504761317E-5	1.6952635015871056E-5
5	0.0	5.085790504761317E-5	0.0	5.93342225555487E-5	1.6952635015871056E-5
6	1.1019212760316187E-4	5.085790504761317E-5	0.0	6.781054006348423E-5	2.5428952523806586E-5
7	1.1019212760316187E-4	6.781054006348423E-5	0.0	6.781054006348423E-5	2.5428952523806586E-5
8	1.1019212760316187E-4	8.476317507935528E-5	0.0	8.476317507935528E-5	2.5428952523806586E-5
9	1.1019212760316187E-4	2.5428952523806587E-4	0.0	1.1019212760316187E-4	2.5428952523806586E-5
10-11	1.1019212760316187E-4	2.5428952523806587E-4	0.0	1.186684451110974E-4	2.5428952523806586E-5
12-13	1.1019212760316187E-4	2.5428952523806587E-4	0.0	1.525737151428395E-4	2.5428952523806586E-5
14-15	1.1019212760316187E-4	3.1362374779361454E-4	0.0	1.9495530268251715E-4	4.661974629364541E-5
16-17	1.6952635015871057E-4	3.1362374779361454E-4	0.0	2.0766977894442045E-4	5.93342225555487E-5
18-19	1.6952635015871057E-4	3.517671765793244E-4	0.0	2.2038425520632375E-4	6.781054006348423E-5
20-21	1.6952635015871057E-4	3.899106053650343E-4	0.0	2.415750489761626E-4	6.781054006348423E-5
22-23	1.7376450891267833E-4	4.32292192904712E-4	0.0	2.966711127777435E-4	6.781054006348423E-5
24-25	1.7800266766664609E-4	4.7043562169042186E-4	0.0	5.467224792618416E-4	7.628685757141975E-5
26-27	1.7800266766664609E-4	4.916264154602607E-4	0.0	0.0010595396884919412	1.0171581009522634E-4
28-29	1.7800266766664609E-4	4.916264154602607E-4	0.0	0.0046534983118566055	1.0171581009522634E-4
30-31	1.7800266766664609E-4	5.340080029999383E-4	0.0	0.01656696256925999	1.1019212760316187E-4
32-33	1.7800266766664609E-4	5.382461617539061E-4	0.0	0.0422290138245348	1.313829213730007E-4
34-35	1.7800266766664609E-4	6.314856543411969E-4	0.0	0.06637804240464312	1.44097397634904E-4
36-37	1.7800266766664609E-4	7.289633056824555E-4	0.0	0.09829985413952833	1.44097397634904E-4
38-39	1.7800266766664609E-4	7.501540994522943E-4	0.0	0.12956475126754852	1.525737151428395E-4
40-41	1.7800266766664609E-4	8.222027982697463E-4	0.0	0.15399773648417267	1.525737151428395E-4
42-43	1.991934614364849E-4	8.433935920395851E-4	0.0	0.17844343617705877	1.5681187389680727E-4
44-45	2.034316201904527E-4	9.705383546586181E-4	0.0	0.19668023329538203	1.652881914047428E-4
46-47	2.0766977894442045E-4	0.0010680160059998766	0.0	0.21207746404854694	1.7376450891267833E-4
48-49	2.119079376983882E-4	0.0010764923235078122	0.0	0.222850863601133	1.8647898517458163E-4
50-51	2.2886057271425926E-4	0.001220589721142716	0.0	0.23167471012689386	1.8647898517458163E-4
52-53	2.2886057271425926E-4	0.0012333041974046193	0.0	0.23859138521336926	2.034316201904527E-4
54-55	2.2886057271425926E-4	0.0012460186736665227	0.0	0.24420694556237654	2.2038425520632375E-4
56-57	2.3309873146822702E-4	0.0012629713086823938	0.0	0.2491147333994712	2.2038425520632375E-4
58-59	2.4581320773013035E-4	0.0012629713086823938	0.0	0.25348427507481197	2.246224139602915E-4
60-61	2.585276839920336E-4	0.0012799239436982648	0.0	0.2575613837961289	2.4581320773013035E-4
62-63	2.627658427460014E-4	0.0012884002612062004	0.0	0.26124434375332695	2.4581320773013035E-4
64-65	2.627658427460014E-4	0.0014664029288728464	0.0	0.26453315494640595	2.500513664840981E-4
66-67	2.627658427460014E-4	0.0014664029288728464	0.0	0.2677075358531278	2.585276839920336E-4
68-69	2.712421602539369E-4	0.001508784516412524	0.0	0.2705216732657624	2.627658427460014E-4
70-71	2.712421602539369E-4	0.0015511661039522018	0.0	0.2730942356294208	2.966711127777435E-4
72-73	2.7548031900790466E-4	0.0015681187389680728	0.0	0.2751878860538809	2.966711127777435E-4
74-75	2.88194795269808E-4	0.0016062621677537827	0.0	0.27701029431808705	2.966711127777435E-4
76-77	2.966711127777435E-4	0.0016571200728013958	0.0	0.27873522493095193	3.05147430285679E-4
78-79	2.966711127777435E-4	0.0016825490253252024	0.0	0.27996429096960257	3.1362374779361454E-4
80-81	2.966711127777435E-4	0.0016952635015871059	0.0	0.28066782532276124	3.3057638280948557E-4
82-83	2.966711127777435E-4	0.0017037398190950413	0.0	0.2811721662144834	3.814342878570988E-4
84-85	3.1362374779361454E-4	0.0017206924541109123	0.0	0.2812908346595945	3.814342878570988E-4
86-87	3.1362374779361454E-4	0.0017376450891267833	0.0	0.2813289780883802	3.899106053650343E-4
88	3.1362374779361454E-4	0.0017885029941743964	0.0	0.2813289780883802	4.068632403809054E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9360	0.0	40.812454	1
GTATCAA	16135	0.0	31.846724	1
TCAACGC	17490	0.0	27.759167	4
ATCAACG	18035	0.0	26.968313	3
CAACGCA	18405	0.0	26.331312	5
TATCAAC	19485	0.0	25.634048	2
AACGCAG	19010	0.0	25.557278	6
GTACATG	38725	0.0	21.10402	1
ACGCAGA	23200	0.0	20.81284	7
TACATGG	38985	0.0	20.323753	2
CGCAGAG	24305	0.0	19.864502	8
ACATGGG	40130	0.0	19.132881	3
CATGGGG	30215	0.0	17.075336	4
GCAGAGT	29765	0.0	16.121212	9
AGAGTAC	25280	0.0	15.038052	10-11
CAGAGTA	28540	0.0	14.755956	10-11
GTGGTAT	6905	0.0	13.7695265	1
ATGGGGG	18005	0.0	13.446411	5
GTATAAG	4675	0.0	13.016104	1
GTATTAG	3700	0.0	12.9055605	1
>>END_MODULE