FastQCFastQC Report
Fri 27 May 2016
SRR2140240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8117850
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78800.044.4209631
GTATCAA138950.035.330051
TCAACGC166550.028.6500914
ATCAACG169450.028.159773
CAACGCA170700.027.9535625
TATCAAC176800.027.377782
AACGCAG175900.027.2491076
TGTAGAT121000.025.60151794
GTACATG373150.022.5825061
ACGCAGA215300.022.1827137
TACATGG372950.022.1800382
CGCAGAG221300.021.6006938
ACATGGG379300.021.3784263
GTGTAGA121300.021.0663294
CATGGGG280400.020.0654934
GGTGTAG140750.018.94388292-93
GTGGTAT48350.017.8548491
GCAGAGT268250.017.7240039
ACCGAAC11850.017.3970998
ATGGGGG162300.017.1215215
Produced by FastQC (version 0.11.5)