FastQCFastQC Report
Fri 27 May 2016
SRR2140239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10438181
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86600.037.4603921
GTATCAA142000.030.8583351
TCAACGC146550.028.1815174
ATCAACG152400.027.0997473
CAACGCA155150.026.5354375
AACGCAG158350.026.1088986
TATCAAC168150.025.232912
ACGCAGA187500.021.8877037
GTACATG319050.021.6697521
CGCAGAG195800.021.004248
TACATGG322950.020.8567282
ACATGGG328300.020.0141773
CATGGGG242450.018.1449994
GCAGAGT250100.016.357129
GTGTAGA146700.015.76432494
AGAGTAC211700.014.8931110-11
ATGGGGG142250.014.7762015
CAGAGTA242100.014.71815810-11
GTGGTAT57800.013.5994211
GTATAGG34600.013.0534981
Produced by FastQC (version 0.11.5)