##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10438181
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78692120782347	33.0	31.0	34.0	30.0	34.0
2	31.875744729852837	33.0	31.0	34.0	30.0	34.0
3	32.00536348239219	34.0	31.0	34.0	30.0	34.0
4	35.48276706449141	37.0	35.0	37.0	33.0	37.0
5	35.54660309109413	37.0	35.0	37.0	33.0	37.0
6	35.56673399321203	37.0	35.0	37.0	33.0	37.0
7	35.507077717851416	37.0	35.0	37.0	33.0	37.0
8	35.48865142307841	37.0	35.0	37.0	33.0	37.0
9	37.08169277769757	39.0	37.0	39.0	33.0	39.0
10-11	37.06276649159466	39.0	37.0	39.0	33.0	39.0
12-13	37.00823357058093	39.0	37.0	39.0	33.0	39.0
14-15	38.37007353101082	40.0	38.0	41.0	33.0	41.0
16-17	38.375859021797	40.0	38.0	41.0	33.0	41.0
18-19	38.377434392065055	40.0	38.0	41.0	33.0	41.0
20-21	38.44396505694469	40.0	38.0	41.0	33.0	41.0
22-23	38.420465407084166	40.0	38.0	41.0	33.0	41.0
24-25	38.430621366822805	40.0	38.0	41.0	33.0	41.0
26-27	38.41284517983826	40.0	38.0	41.0	33.0	41.0
28-29	38.386236887403726	40.0	38.0	41.0	33.0	41.0
30-31	38.33319224470296	40.0	38.0	41.0	33.0	41.0
32-33	38.29336836999357	40.0	38.0	41.0	33.0	41.0
34-35	38.232779178206705	40.0	38.0	41.0	33.0	41.0
36-37	38.16839491914166	40.0	38.0	41.0	33.0	41.0
38-39	38.08658231937345	40.0	38.0	41.0	33.0	41.0
40-41	38.013032067754736	40.0	38.0	41.0	32.5	41.0
42-43	37.91755984435109	40.0	37.0	41.0	32.0	41.0
44-45	37.78105900272722	40.0	37.0	41.0	32.0	41.0
46-47	37.67463554903877	40.0	37.0	41.0	32.0	41.0
48-49	37.56589885407003	40.0	36.5	41.0	31.0	41.0
50-51	37.42625005321969	40.0	36.0	41.0	31.0	41.0
52-53	37.30316521728276	39.0	36.0	41.0	31.0	41.0
54-55	37.16107062519443	39.0	36.0	41.0	31.0	41.0
56-57	36.98203556159267	39.0	35.0	40.5	31.0	41.0
58-59	37.13851350215249	39.0	35.0	41.0	31.0	41.0
60-61	37.21094464349392	39.0	35.0	41.0	31.0	41.0
62-63	37.05470121600817	39.0	35.0	41.0	31.0	41.0
64-65	36.84948126487433	38.5	35.0	40.0	31.0	41.0
66-67	36.579485788242465	38.0	35.0	40.0	31.0	41.0
68-69	36.30198135569663	37.0	35.0	40.0	31.0	41.0
70-71	36.06476634799421	37.0	35.0	39.5	30.5	41.0
72-73	35.82701142721562	36.5	34.0	39.0	30.0	41.0
74-75	35.556885331566946	36.0	34.0	39.0	30.0	41.0
76-77	35.308453582507184	35.5	34.0	38.5	30.0	40.0
78-79	34.98743665986544	35.0	34.0	37.0	30.0	39.5
80-81	34.694188064615744	35.0	34.0	37.0	30.0	39.0
82-83	34.421267779129394	35.0	34.0	36.5	30.0	39.0
84-85	34.198321393213135	35.0	34.0	36.0	30.0	37.0
86-87	33.97708644466275	35.0	34.0	36.0	30.0	37.0
88-89	33.82108129116081	35.0	34.0	35.0	30.0	37.0
90-91	33.69759127273507	35.0	34.0	35.0	30.0	36.0
92-93	33.603310615691214	35.0	34.0	35.0	30.0	36.0
94-95	33.567210173000944	35.0	34.0	35.0	30.0	36.0
96-97	33.573143215711205	35.0	34.0	35.0	30.0	36.0
98-99	33.61870322098612	35.0	34.0	35.0	30.0	36.0
100	33.26922186649089	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	2.0
16	50.0
17	348.0
18	1412.0
19	4282.0
20	10858.0
21	22477.0
22	37149.0
23	52428.0
24	69595.0
25	86133.0
26	101636.0
27	112565.0
28	129078.0
29	157574.0
30	188712.0
31	220027.0
32	274781.0
33	359876.0
34	486345.0
35	679914.0
36	1056713.0
37	1909057.0
38	2786069.0
39	1604908.0
40	86190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.125747867372674	20.378215323148734	14.472291676107169	24.023745133371417
2	14.101422460484256	20.752131046587525	42.14671119422053	22.999735298707698
3	17.959671325875647	25.948793185326064	30.2357182731359	25.855817215662384
4	12.4504930504654	17.82704285353933	39.109563246699786	30.61290084929549
5	13.480829912313114	37.89374624634622	35.513739342832636	13.111684498508023
6	30.10703684866166	37.4662980072869	19.22827358521566	13.198391558835779
7	26.81659063170016	32.49669961621662	23.1839266998653	17.502783052217914
8	24.259102232467516	36.931290997923874	21.351258423282754	17.45834834632586
9	25.32360762857053	17.742334607916842	21.539749119123343	35.39430864438929
10-11	23.963825292481626	26.97252115958563	29.06865825325629	19.994995294676453
12-13	24.54566731358746	24.539799424534923	29.040829586346874	21.873703675530738
14-15	21.943992524365843	26.122791736258954	26.311330387353042	25.62188535202216
16-17	21.89134311336868	29.186764978284746	27.014377377182846	21.907514531163724
18-19	21.941163694967244	28.843635883575896	28.042248003679603	21.172952417777253
20-21	22.808672216858618	27.92373889608163	27.785139853478956	21.482449033580796
22-23	22.60718107405299	27.782436839263912	27.578075967304372	22.032306119378728
24-25	22.28684866943883	27.948208635032962	27.612688107875687	22.15225458765252
26-27	22.201061370914772	28.11015077342733	27.562417399385225	22.12637045627267
28-29	22.16198389362481	28.120668501660262	27.606679861423792	22.11066774329114
30-31	22.418948692978162	27.94589646614866	27.542873701486965	22.092281139386216
32-33	22.197415744121468	28.040123175263947	27.55940837804532	22.203052702569266
34-35	22.357788130251556	28.010454223856545	27.576377172809828	22.05538047308207
36-37	22.28901536818527	27.818930009212682	27.630184463238905	22.261870159363138
38-39	22.110978474709967	27.872901525851233	27.827898033915428	22.18822196552337
40-41	22.33806699756488	27.697664665063808	27.793179136154695	22.171089201216613
42-43	22.055173743794935	27.72292319151176	27.793471217834693	22.428431846858615
44-45	22.136805905856715	27.77629143977392	27.80447181609496	22.2824308382744
46-47	22.02051624316301	27.719079560298876	27.815599440759314	22.4448047557788
48-49	21.87201455179108	27.738602453202034	27.896437675467865	22.49294531953902
50-51	21.802042103767782	27.819746128542537	27.926652891318284	22.451558876371397
52-53	21.95380475607465	27.70519191851904	27.844826380422983	22.49617694498333
54-55	21.970942873996123	27.48292995030937	27.907185622857156	22.638941552837355
56-57	21.868497109169873	27.385834288561306	27.948407641157246	22.79726096111158
58-59	21.802658575563793	27.28767405443143	28.09092009958353	22.818747270421245
60-61	21.796895147830426	27.12812399138116	28.36855571584503	22.706425144943392
62-63	21.730386806759576	27.322537526721607	28.30267523019453	22.644400436324286
64-65	21.679559071295326	27.540741854387107	27.981896329006332	22.797802745311234
66-67	21.456174561658585	27.786269578825145	27.773458054276002	22.98409780524027
68-69	21.536911384301796	27.918433421114347	27.733697122381113	22.810958072202748
70-71	21.55694078566946	27.975487204994277	27.500408893828105	22.967163115508157
72-73	21.813511057980573	27.805150799436568	27.148803870882315	23.232534271700548
74-75	21.99039709331431	27.6990772417808	27.251647242499043	23.058878422405847
76-77	22.150830125176153	27.29309809547552	27.39925603701957	23.156815742328753
78-79	22.38704022851586	27.05392483045988	27.35669972436015	23.20233521666411
80-81	22.757312610216687	27.05948232742708	27.00224159285109	23.180963469505144
82-83	22.50581438712347	27.110531066736204	27.15247462754884	23.23117991859149
84-85	22.592530745319948	27.31134505725217	27.117970386529834	22.978153810898053
86-87	22.385025168525633	27.592940101711033	27.250975076841932	22.7710596529214
88-89	22.110478458991185	27.68078258904383	27.28628842758815	22.922450524376835
90-91	21.997439171318288	27.86956610700817	27.46867083102845	22.66432389064509
92-93	21.972574234570036	27.474409451939547	27.988122761463448	22.56489355202697
94-95	21.646233832523087	27.463838079202702	28.383632150395457	22.506295937878757
96-97	22.01288400411864	27.38161940288596	28.25512445558051	22.35037213741489
98-99	23.36803666384873	29.301080512252476	28.587625909551384	18.743256914347405
100	22.503429850530722	30.539601671484373	22.291160535639	24.665807942345904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3300.0
1	2492.5
2	1805.0
3	1891.5
4	1869.0
5	2029.5
6	2326.0
7	2684.0
8	3049.5
9	3414.0
10	3876.0
11	4320.5
12	4809.5
13	5429.0
14	6141.0
15	7185.0
16	8615.5
17	10300.0
18	12453.0
19	15187.0
20	18518.0
21	23423.0
22	30101.5
23	38782.5
24	49935.0
25	63705.0
26	81076.5
27	100783.5
28	122722.0
29	148138.5
30	173027.0
31	196256.0
32	220317.0
33	243956.0
34	267373.0
35	288755.0
36	311386.0
37	335118.0
38	353084.5
39	366907.5
40	382692.0
41	397406.0
42	407343.0
43	415926.5
44	423447.0
45	432172.5
46	437480.5
47	438497.0
48	436754.5
49	429825.5
50	422184.0
51	410695.5
52	392641.5
53	370824.5
54	347214.5
55	322537.0
56	296393.5
57	268353.0
58	239358.0
59	208860.5
60	175685.0
61	144028.0
62	115869.0
63	91257.0
64	70400.0
65	54286.0
66	42153.0
67	32884.0
68	25882.0
69	20253.0
70	15721.5
71	11930.5
72	9032.5
73	6805.5
74	5079.5
75	3869.5
76	2967.5
77	2259.5
78	1780.5
79	1412.0
80	1068.5
81	807.5
82	634.0
83	448.0
84	320.0
85	242.0
86	191.0
87	147.0
88	91.5
89	57.0
90	41.5
91	33.5
92	23.5
93	11.5
94	7.0
95	7.5
96	7.5
97	7.0
98	5.5
99	8.5
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.7244383863433677E-4
6	0.0
7	9.580213257463153E-6
8	0.0
9	0.0
10-11	3.832085302985261E-5
12-13	1.437031988619473E-4
14-15	2.874063977238946E-5
16-17	1.1017245246082627E-4
18-19	9.771817522612417E-4
20-21	3.6902098540871856E-4
22-23	0.0
24-25	7.679017454358679E-5
26-27	1.4891085638921725E-4
28-29	0.0
30-31	0.0
32-33	6.274008034687736E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	7.524400502168458E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	2.1601363046008202E-4
76-77	2.7305394453728277E-5
78-79	3.9245884253716573E-4
80-81	4.47892871191497E-5
82-83	1.7015640436376307E-5
84-85	3.102995309880052E-4
86-87	6.990524053373817E-5
88-89	2.363973982102353E-5
90-91	2.4001387760240298E-5
92-93	6.036261100851838E-4
94-95	3.2894677634952123E-4
96-97	3.9399302975492555E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3502.0
20-21	6802.0
22-23	7851.0
24-25	8618.0
26-27	10828.0
28-29	14795.0
30-31	20498.0
32-33	22010.0
34-35	26817.0
36-37	40561.0
38-39	47746.0
40-41	40650.0
42-43	43340.0
44-45	51904.0
46-47	66322.0
48-49	41824.0
50-51	41985.0
52-53	17613.0
54-55	17893.0
56-57	17538.0
58-59	17785.0
60-61	19416.0
62-63	85342.0
64-65	90766.0
66-67	97177.0
68-69	98556.0
70-71	98441.0
72-73	98129.0
74-75	101217.0
76-77	108120.0
78-79	114435.0
80-81	115505.0
82-83	114226.0
84-85	116869.0
86-87	121607.0
88-89	125893.0
90-91	130994.0
92-93	140931.0
94-95	163825.0
96-97	374592.0
98-99	686154.0
100-101	6869104.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.34415805087038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61621703663054	73.85643998571169
2	7.746502738035391	12.912514970806436
3	2.0255660139736613	5.064572820332771
4	0.7410278301927755	2.4704136239872088
5	0.3367806821706991	1.4034351201657345
6	0.18016611810777386	0.9009476053791649
7	0.09867616876789717	0.5756857543952187
8	0.06907331172991395	0.460548560793199
9	0.03999584591892223	0.3000078093280422
>10	0.14233963158123036	1.8097146052366553
>50	0.0031022754127434464	0.17062696329007448
>100	5.326012308071627E-4	0.0657608665896614
>500	1.974624769438511E-5	0.00933131398422649
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.916042651492631E-5	9.580213257463155E-6
2	0.0	1.916042651492631E-5	0.0	1.916042651492631E-5	9.580213257463155E-6
3	0.0	1.916042651492631E-5	0.0	2.874063977238946E-5	9.580213257463155E-6
4	0.0	7.664170605970524E-5	0.0	2.874063977238946E-5	1.916042651492631E-5
5	0.0	7.664170605970524E-5	0.0	3.832085302985262E-5	1.916042651492631E-5
6	0.0	7.664170605970524E-5	0.0	3.832085302985262E-5	2.874063977238946E-5
7	0.0	7.664170605970524E-5	0.0	3.832085302985262E-5	2.874063977238946E-5
8	0.0	8.622191931716838E-5	0.0	4.7901066287315766E-5	3.832085302985262E-5
9	0.0	1.24542772347021E-4	0.0	6.706149280224208E-5	4.7901066287315766E-5
10-11	9.580213257463155E-6	1.2933287897575258E-4	0.0	7.664170605970524E-5	4.7901066287315766E-5
12-13	9.580213257463155E-6	1.437031988619473E-4	0.0	8.622191931716838E-5	5.748127954477892E-5
14-15	2.874063977238946E-5	2.2034490492165253E-4	0.0	1.005922392033631E-4	6.706149280224208E-5
16-17	5.748127954477892E-5	2.2992511817911568E-4	0.0	1.5328341211941047E-4	7.664170605970524E-5
18-19	8.143181268843681E-5	2.538756513227736E-4	0.0	1.9639437177799464E-4	1.1017245246082627E-4
20-21	9.580213257463153E-5	2.9219650435262617E-4	0.0	2.8740639772389465E-4	1.24542772347021E-4
22-23	1.053823458320947E-4	2.9698661098135774E-4	0.0	5.700226888190577E-4	1.3891309223321575E-4
24-25	1.3891309223321572E-4	3.353074640112104E-4	0.0	0.0013747606024459625	1.676537320056052E-4
26-27	1.7723394526306834E-4	3.544678905261367E-4	0.0	0.0033195438937109827	1.7244383863433677E-4
28-29	1.8202405189179992E-4	3.5925799715486826E-4	0.0	0.014993033747929835	1.7244383863433677E-4
30-31	1.8202405189179992E-4	4.550601297294998E-4	0.0	0.05397971159917614	1.7244383863433677E-4
32-33	2.0118447840672624E-4	4.6464034298696294E-4	0.0	0.15478271549420344	2.0118447840672624E-4
34-35	2.107646916641894E-4	5.31701835789205E-4	0.0	0.2891404163234954	2.2992511817911568E-4
36-37	2.2992511817911568E-4	5.939732219627155E-4	0.0	0.4783687885849076	2.2992511817911568E-4
38-39	2.49085544694042E-4	6.035534352201786E-4	0.0	0.6980526587917952	2.538756513227736E-4
40-41	2.49085544694042E-4	6.801951412798839E-4	0.0	0.9067384441791151	2.682459712089683E-4
42-43	2.49085544694042E-4	7.185159943097365E-4	0.0	1.2165098497525575	2.730360778376999E-4
44-45	2.682459712089683E-4	8.047379136269048E-4	0.0	1.4884777338120503	2.7782618446643145E-4
46-47	2.7782618446643145E-4	8.430587666567575E-4	0.0	1.7578637503986565	2.9219650435262617E-4
48-49	2.7782618446643145E-4	8.478488732854891E-4	0.0	1.9204016485247766	2.9698661098135774E-4
50-51	2.7782618446643145E-4	9.484411124888522E-4	0.0	2.3045538298291626	2.9698661098135774E-4
52-53	2.82616291095163E-4	9.628114323750471E-4	0.0	2.7822472133794194	2.9698661098135774E-4
54-55	2.874063977238946E-4	9.723916456325101E-4	0.0	3.3946910865025237	2.9698661098135774E-4
56-57	2.874063977238946E-4	9.771817522612417E-4	0.0	4.058556754285062	3.017767176100893E-4
58-59	2.874063977238946E-4	0.0010011322854048994	0.0	4.735427561564606	3.0656682423882095E-4
60-61	3.113569308675525E-4	0.0010490333516922153	0.0	5.402622353454112	3.0656682423882095E-4
62-63	3.161470374962841E-4	0.0010777739914646048	0.0	5.896650000608344	3.161470374962841E-4
64-65	3.161470374962841E-4	0.001178366230667968	0.0	5.90636912695804	3.161470374962841E-4
66-67	3.4009757063994196E-4	0.001197526657182894	0.0	5.910268273753827	3.2093714412501567E-4
68-69	3.544678905261367E-4	0.0012406376168414784	0.0	5.913880014151891	3.305173573824788E-4
70-71	3.544678905261367E-4	0.0012597980433564047	0.0	5.916993583460567	3.4488767726867354E-4
72-73	3.6404810378359983E-4	0.001269378256613868	0.0	5.919733524452202	3.544678905261367E-4
74-75	3.6404810378359983E-4	0.001288538683128794	0.0	5.922109417340051	3.544678905261367E-4
76-77	3.6404810378359983E-4	0.001322069429529915	0.0	5.924432619054986	3.544678905261367E-4
78-79	3.73628317041063E-4	0.0013508100693023046	0.0	5.9261474772280724	3.544678905261367E-4
80-81	3.8320853029852613E-4	0.0013508100693023046	0.0	5.927071967807418	3.6404810378359983E-4
82-83	3.8799863692725776E-4	0.0013699704958172309	0.0	5.927565348790177	4.023689568134525E-4
84-85	3.975788501847209E-4	0.0013795507090746941	0.0	5.927785693695099	4.0715906344218405E-4
86-87	4.119491700709156E-4	0.0013987111355896204	0.0	5.927852755187901	4.119491700709156E-4
88	4.119491700709156E-4	0.0014466122018769363	0.0	5.927862335401159	4.119491700709156E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8660	0.0	37.460392	1
GTATCAA	14200	0.0	30.858335	1
TCAACGC	14655	0.0	28.181517	4
ATCAACG	15240	0.0	27.099747	3
CAACGCA	15515	0.0	26.535437	5
AACGCAG	15835	0.0	26.108898	6
TATCAAC	16815	0.0	25.23291	2
ACGCAGA	18750	0.0	21.887703	7
GTACATG	31905	0.0	21.669752	1
CGCAGAG	19580	0.0	21.00424	8
TACATGG	32295	0.0	20.856728	2
ACATGGG	32830	0.0	20.014177	3
CATGGGG	24245	0.0	18.144999	4
GCAGAGT	25010	0.0	16.35712	9
GTGTAGA	14670	0.0	15.764324	94
AGAGTAC	21170	0.0	14.89311	10-11
ATGGGGG	14225	0.0	14.776201	5
CAGAGTA	24210	0.0	14.718158	10-11
GTGGTAT	5780	0.0	13.599421	1
GTATAGG	3460	0.0	13.053498	1
>>END_MODULE