FastQCFastQC Report
Fri 27 May 2016
SRR2140238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10602836
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88600.037.8656231
GTATCAA147450.031.8365611
TCAACGC158600.028.1238084
ATCAACG165400.026.9932773
CAACGCA166650.026.816325
AACGCAG172200.025.9520366
TATCAAC177800.025.5890332
ACGCAGA204650.021.8162147
GTACATG351100.021.532911
CGCAGAG214050.020.8978848
TACATGG354300.020.6183452
ACATGGG362600.019.5600553
CATGGGG265550.017.3574054
GCAGAGT269200.016.5218419
GTGGTAT58400.016.0909331
AGAGTAC226400.015.13775410-11
GTGTAGC55500.014.7865261
CAGAGTA259750.014.68384310-11
ATGGGGG142900.013.9554045
AGGACCG21900.013.7852955
Produced by FastQC (version 0.11.5)