##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10602836
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754033826421534	33.0	31.0	34.0	30.0	34.0
2	31.833386652401302	33.0	31.0	34.0	30.0	34.0
3	31.964462809761464	34.0	31.0	34.0	30.0	34.0
4	35.4480544639189	37.0	35.0	37.0	33.0	37.0
5	35.512117135453195	37.0	35.0	37.0	33.0	37.0
6	35.533573376028826	37.0	35.0	37.0	33.0	37.0
7	35.46648208083196	37.0	35.0	37.0	33.0	37.0
8	35.449722979776354	37.0	35.0	37.0	33.0	37.0
9	37.03552172267872	39.0	37.0	39.0	33.0	39.0
10-11	37.01267901342622	39.0	37.0	39.0	33.0	39.0
12-13	36.95562559866059	39.0	37.0	39.0	33.0	39.0
14-15	38.30776671449035	40.0	38.0	41.0	33.0	41.0
16-17	38.313642406616495	40.0	38.0	41.0	33.0	41.0
18-19	38.31541433820159	40.0	38.0	41.0	33.0	41.0
20-21	38.379954635131654	40.0	38.0	41.0	33.0	41.0
22-23	38.35951810098428	40.0	38.0	41.0	33.0	41.0
24-25	38.371365727802875	40.0	38.0	41.0	33.0	41.0
26-27	38.35412315696907	40.0	38.0	41.0	33.0	41.0
28-29	38.32644999823758	40.0	38.0	41.0	33.0	41.0
30-31	38.273784980116886	40.0	38.0	41.0	33.0	41.0
32-33	38.23288888159915	40.0	38.0	41.0	33.0	41.0
34-35	38.1696955147992	40.0	38.0	41.0	33.0	41.0
36-37	38.10470689655797	40.0	38.0	41.0	33.0	41.0
38-39	38.01938411690837	40.0	38.0	41.0	32.0	41.0
40-41	37.94271944471772	40.0	37.0	41.0	32.0	41.0
42-43	37.84470092493254	40.0	37.0	41.0	32.0	41.0
44-45	37.70338327364723	40.0	37.0	41.0	32.0	41.0
46-47	37.5909418763616	40.0	37.0	41.0	31.0	41.0
48-49	37.47889384115442	40.0	36.0	41.0	31.0	41.0
50-51	37.33114314600314	39.5	36.0	41.0	31.0	41.0
52-53	37.19874499231091	39.0	36.0	41.0	31.0	41.0
54-55	37.044901409353315	39.0	35.0	41.0	30.5	41.0
56-57	36.85042046810503	39.0	35.0	40.0	30.0	41.0
58-59	37.00443175076599	39.0	35.0	40.5	31.0	41.0
60-61	37.07061375445842	39.0	35.0	41.0	31.0	41.0
62-63	36.90325675961486	39.0	35.0	40.5	31.0	41.0
64-65	36.68993920903254	38.0	35.0	40.0	31.0	41.0
66-67	36.407190307324605	37.5	35.0	40.0	31.0	41.0
68-69	36.12343084783224	37.0	34.5	40.0	30.0	41.0
70-71	35.88699263028104	37.0	34.0	39.0	30.0	41.0
72-73	35.649623000379194	36.0	34.0	39.0	30.0	41.0
74-75	35.38500823567321	36.0	34.0	39.0	30.0	40.5
76-77	35.143977164271426	35.0	34.0	38.0	30.0	40.0
78-79	34.85284191615325	35.0	34.0	37.0	30.0	39.0
80-81	34.58375821014444	35.0	34.0	37.0	30.0	39.0
82-83	34.336168154668954	35.0	34.0	36.0	30.0	38.5
84-85	34.1323820534174	35.0	34.0	36.0	30.0	37.0
86-87	33.96023266222042	35.0	34.0	36.0	30.0	37.0
88-89	33.83365563273867	35.0	34.0	35.0	30.0	37.0
90-91	33.73937222414443	35.0	34.0	35.0	30.0	36.0
92-93	33.666245147979055	35.0	34.0	35.0	30.0	36.0
94-95	33.6505173799874	35.0	34.0	35.0	30.0	36.0
96-97	33.659356970423325	35.0	34.0	35.0	30.0	36.0
98-99	33.698089013208104	35.0	34.0	35.0	30.0	35.5
100	33.36111756875116	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	5.0
16	49.0
17	362.0
18	1493.0
19	4317.0
20	11220.0
21	23094.0
22	38125.0
23	54747.0
24	72937.0
25	91479.0
26	106770.0
27	118719.0
28	136685.0
29	166590.0
30	196913.0
31	227023.0
32	282749.0
33	366509.0
34	492778.0
35	684084.0
36	1059133.0
37	1905842.0
38	2742407.0
39	1722518.0
40	96287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.642254958956265	20.012334435805666	14.24158593040579	24.103824674832282
2	14.334334700640468	20.655068134600967	41.82790340244817	23.182693762310386
3	18.13396906261683	25.703472165371604	30.057128111761795	26.105430660249766
4	12.524498162567072	17.666377184368407	38.80120375341088	31.00792089965364
5	13.64149091628625	37.79331577951606	35.27316595525229	13.292027348945403
6	30.45492734208093	37.16526408594832	19.073198906405793	13.30660966556495
7	27.19167777375789	32.34164897014346	22.843256275962393	17.62341698013626
8	24.641581675238903	36.5512560132508	21.106687136665535	17.70047517484476
9	25.62337095471438	17.430676094584506	21.17070376265369	35.775249188047425
10-11	24.24373378410605	26.790011403087128	28.809839976221447	20.15641483658538
12-13	24.836647200772475	24.382682981071635	28.696427685545483	22.084242132610406
14-15	22.19306491920909	25.872548679798534	26.094168704447608	25.840217696544766
16-17	22.12289803601343	28.925162565211416	26.749105096737452	22.2028343020377
18-19	22.179648791280243	28.5600265177075	27.77299310700884	21.487331584003417
20-21	23.191121913506052	27.64449436520656	27.470359377115084	21.694024344172306
22-23	22.875075537608936	27.544703226609712	27.25131111664782	22.328910119133532
24-25	22.543643758778767	27.701417521425558	27.363076740758213	22.391861979037465
26-27	22.474797325348213	27.845478142106224	27.28298249913711	22.396742033408458
28-29	22.43259899232608	27.85509307169135	27.3461043371742	22.36620359880837
30-31	22.717152928334432	27.672308737902114	27.26766209118833	22.34287624257512
32-33	22.47664910352205	27.7973727380954	27.26858684023134	22.45739131815121
34-35	22.63744469944782	27.739127116729286	27.30156742995708	22.321860753865817
36-37	22.5813030195534	27.55001307331454	27.346502276744484	22.522181630387582
38-39	22.373340631206155	27.640098060445645	27.567319348485313	22.419241959862884
40-41	22.6217213465744	27.455416344505736	27.51585755521165	22.407004753708218
42-43	22.328007151762783	27.49718738105072	27.530186644298183	22.644618822888315
44-45	22.3604715773693	27.553806228255624	27.559324740768847	22.526397453606226
46-47	22.299166419143873	27.537291852635953	27.471054557033668	22.6924871711865
48-49	22.18951055288352	27.572171576210426	27.583275789349614	22.655042081556438
50-51	22.155053069218162	27.68379904923397	27.5384687232111	22.622679158336766
52-53	22.28376198868693	27.619143439239785	27.434709621383558	22.66238495068973
54-55	22.373095229198626	27.461091625926194	27.43243106772193	22.73338207715325
56-57	22.356578655196742	27.556235225269244	27.348809942811563	22.738376176722454
58-59	22.32232652274034	27.50907746643989	27.433257926395672	22.735338084424097
60-61	22.434597627400315	27.46742834760051	27.429098236177246	22.668875788821925
62-63	22.450918958831448	27.616012587651444	27.251467973317794	22.68160048019931
64-65	22.531201875347794	27.572604097146865	27.135306417113288	22.760887610392057
66-67	22.562512926698442	27.6114438820671	27.03477706100932	22.791266130225136
68-69	22.50033964297119	27.558574052873418	27.13645136816926	22.804634935986133
70-71	22.594348676762806	27.360761812942926	27.222254334629703	22.822635175664562
72-73	22.6616225739562	27.357494308771123	27.150880892943142	22.830002224329533
74-75	22.57194880438664	27.544786570820428	27.237856454130778	22.645408170662158
76-77	22.692002907030385	27.490640153303588	27.179148427895054	22.638208511770973
78-79	22.646888265494187	27.47242400738317	27.165999952437442	22.714687774685196
80-81	22.6478450108095	27.562245536318386	27.174291558332612	22.6156178945395
82-83	22.66178027415654	27.507856971073974	27.35600283048973	22.474359924279756
84-85	22.648377839203622	27.601936218780594	27.28940034420529	22.460285597810497
86-87	22.475954197758504	27.67464525505855	27.37471496231646	22.47468558486649
88-89	22.551590970943522	27.64278501005968	27.32576313798951	22.479860881007287
90-91	22.534766366558102	27.713726113227278	27.334378054680915	22.417129465533712
92-93	22.575214766655993	27.657659183076746	27.380976449398513	22.386149600868745
94-95	22.414264561893358	27.796228399331728	27.361984493941055	22.427522544833852
96-97	22.45928911140271	27.881813961528568	27.453415472149928	22.205481454918793
98-99	23.512191622195107	29.843873322862557	27.631103468770917	19.012831586171416
100	22.457313585479184	30.863434460226035	21.355714208087168	25.32353774620761
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3576.0
1	2662.0
2	1916.5
3	2084.0
4	2045.0
5	2153.0
6	2488.5
7	2913.0
8	3277.5
9	3688.5
10	4208.0
11	4660.0
12	5179.0
13	5845.5
14	6669.5
15	7771.0
16	9147.5
17	10744.5
18	12774.5
19	15594.5
20	19333.0
21	24231.0
22	30993.0
23	39888.0
24	51150.5
25	66084.0
26	84942.0
27	105337.5
28	126275.5
29	150183.5
30	173398.5
31	195496.0
32	220101.5
33	244011.5
34	267963.0
35	290424.5
36	314261.5
37	337734.0
38	353818.0
39	367949.5
40	384929.0
41	399778.5
42	410792.0
43	422068.5
44	431646.0
45	441440.5
46	449371.5
47	454370.5
48	456850.0
49	454262.0
50	448721.0
51	438729.0
52	421549.0
53	397826.5
54	373566.0
55	349784.0
56	324920.0
57	297209.5
58	267910.5
59	237242.0
60	201889.0
61	167311.0
62	135887.5
63	108176.5
64	84943.0
65	65962.0
66	51251.5
67	39647.5
68	30625.5
69	23970.5
70	18677.0
71	14099.0
72	10563.5
73	7747.0
74	5645.5
75	4190.0
76	3157.0
77	2419.0
78	1924.5
79	1576.0
80	1235.5
81	924.5
82	693.5
83	482.5
84	339.5
85	249.0
86	192.0
87	156.5
88	108.5
89	75.0
90	59.5
91	50.0
92	41.5
93	23.0
94	11.0
95	9.5
96	9.0
97	8.5
98	5.0
99	5.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.3204014473108892E-4
6	0.0
7	0.0
8	1.8862877818726992E-5
9	0.0
10-11	5.187291400149922E-5
12-13	1.697659003685429E-4
14-15	3.301003618277223E-5
16-17	1.3204014473108892E-4
18-19	0.0010327425605753027
20-21	5.190004410088293E-4
22-23	0.0
24-25	1.370296239618884E-4
26-27	1.8917873067314617E-4
28-29	0.0
30-31	0.0
32-33	7.128987527883846E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	5.476645120714968E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.9516265385299312E-4
76-77	5.642965721579006E-6
78-79	4.4589697607534055E-4
80-81	2.8975999758919683E-5
82-83	4.1130573706869454E-5
84-85	4.765878312948183E-4
86-87	1.2082012945514411E-4
88-89	3.064078084475652E-5
90-91	2.4882210724705585E-5
92-93	4.8016319483405056E-4
94-95	3.5328802596397207E-4
96-97	3.676550481762701E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3794.0
20-21	7077.0
22-23	8176.0
24-25	9141.0
26-27	11592.0
28-29	15841.0
30-31	21390.0
32-33	23260.0
34-35	28636.0
36-37	44692.0
38-39	52442.0
40-41	47970.0
42-43	56402.0
44-45	67094.0
46-47	73681.0
48-49	70339.0
50-51	69784.0
52-53	70623.0
54-55	77192.0
56-57	86369.0
58-59	89703.0
60-61	89448.0
62-63	89250.0
64-65	94005.0
66-67	99192.0
68-69	101177.0
70-71	101507.0
72-73	100942.0
74-75	104034.0
76-77	112065.0
78-79	118297.0
80-81	119453.0
82-83	116499.0
84-85	115531.0
86-87	117257.0
88-89	120045.0
90-91	124043.0
92-93	127103.0
94-95	145046.0
96-97	357012.0
98-99	635445.0
100-101	6680287.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.97144198765552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79606267370883	76.3010093019893
2	7.08502741148187	12.040499913513617
3	1.716014906418749	4.374367832121387
4	0.651749767247673	2.215204701526145
5	0.2893028913369375	1.2291241924048797
6	0.1530067869305962	0.7800724391634434
7	0.08666113186832242	0.5154604937393606
8	0.06113858848751237	0.41560272198990406
9	0.03720521332735762	0.2845242563295524
>10	0.12057661400693334	1.5906353396027881
>50	0.002560952866937512	0.1460236486820635
>100	6.745746430274948E-4	0.09215864569246922
>500	1.848767537248971E-5	0.015316513245151141
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.772575563745398E-5	0.0	9.431438909363494E-6	0.0
3	0.0	4.7157194546817474E-5	0.0	9.431438909363494E-6	0.0
4	0.0	8.488295018427145E-5	0.0	5.658863345618097E-5	0.0
5	0.0	8.488295018427145E-5	0.0	8.488295018427145E-5	0.0
6	0.0	8.488295018427145E-5	0.0	8.488295018427145E-5	0.0
7	0.0	8.488295018427145E-5	0.0	8.488295018427145E-5	0.0
8	9.431438909363494E-6	8.488295018427145E-5	0.0	9.431438909363495E-5	0.0
9	9.431438909363494E-6	1.6033446145917942E-4	0.0	1.0374582800299844E-4	0.0
10-11	1.886287781872699E-5	1.6033446145917942E-4	0.0	1.2260870582172544E-4	0.0
12-13	1.886287781872699E-5	1.6033446145917942E-4	0.0	1.3204014473108892E-4	0.0
14-15	1.886287781872699E-5	2.3578597273408736E-4	0.0	1.3675586418577067E-4	2.8294316728090485E-5
16-17	2.8294316728090485E-5	2.3578597273408736E-4	0.0	1.509030225498159E-4	2.8294316728090485E-5
18-19	2.8294316728090485E-5	2.499331310981326E-4	0.0	1.5561874200449767E-4	2.8294316728090485E-5
20-21	2.8294316728090485E-5	2.7351172837154135E-4	0.0	2.687960089168596E-4	2.8294316728090485E-5
22-23	2.8294316728090485E-5	3.018060450996318E-4	0.0	6.790636014741716E-4	3.301003618277223E-5
24-25	5.6588633456180964E-5	3.348160812824041E-4	0.0	0.0014288629947685695	4.7157194546817474E-5
26-27	6.602007236554446E-5	3.5367895910113105E-4	0.0	0.003532073871556629	4.7157194546817474E-5
28-29	6.602007236554446E-5	3.5839467855581283E-4	0.0	0.016986021475763655	4.7157194546817474E-5
30-31	6.602007236554446E-5	3.9612043419326676E-4	0.0	0.05904080757261548	4.7157194546817474E-5
32-33	6.602007236554446E-5	3.9612043419326676E-4	0.0	0.16113141804702064	5.658863345618097E-5
34-35	6.602007236554446E-5	4.4327762874008425E-4	0.0	0.2946428672479703	5.658863345618097E-5
36-37	6.602007236554446E-5	4.998662621962652E-4	0.0	0.4804374980429764	5.658863345618097E-5
38-39	6.602007236554446E-5	5.092977011056288E-4	0.0	0.6846422975890601	5.658863345618097E-5
40-41	6.602007236554446E-5	5.658863345618097E-4	0.0	0.8760344873767736	5.658863345618097E-5
42-43	6.602007236554446E-5	5.753177734711732E-4	0.0	1.1269060466463878	5.658863345618097E-5
44-45	7.073579182022621E-5	6.460535652913994E-4	0.0	1.3133891724817774	5.658863345618097E-5
46-47	7.545151127490796E-5	7.073579182022621E-4	0.0	1.4826835009048522	6.130435291086272E-5
48-49	7.545151127490796E-5	7.073579182022621E-4	0.0	1.6046461531612861	7.545151127490796E-5
50-51	7.545151127490796E-5	8.063880267505789E-4	0.0	1.7515172355773494	7.545151127490796E-5
52-53	7.545151127490796E-5	8.205351851146241E-4	0.0	1.8935311269550903	7.545151127490796E-5
54-55	7.545151127490796E-5	8.205351851146241E-4	0.0	2.0227937129273714	7.545151127490796E-5
56-57	7.545151127490796E-5	8.205351851146241E-4	0.0	2.126643286758373	7.545151127490796E-5
58-59	8.01672307295897E-5	8.205351851146241E-4	0.0	2.1746917522821256	7.545151127490796E-5
60-61	8.488295018427145E-5	8.205351851146241E-4	0.0	2.1928519879021047	8.488295018427145E-5
62-63	8.488295018427145E-5	8.205351851146241E-4	0.0	2.2040235272902455	8.488295018427145E-5
64-65	8.488295018427145E-5	8.865552574801685E-4	0.0	2.208446872138737	8.488295018427145E-5
66-67	8.488295018427145E-5	9.054181352988955E-4	0.0	2.2124410865168525	8.488295018427145E-5
68-69	8.488295018427145E-5	9.14849574208259E-4	0.0	2.2162655349945997	8.488295018427145E-5
70-71	8.488295018427145E-5	9.289967325723043E-4	0.0	2.2201560035447123	8.488295018427145E-5
72-73	9.431438909363495E-5	9.33712452026986E-4	0.0	2.2234475757240797	1.0374582800299844E-4
74-75	1.0374582800299844E-4	9.478596103910312E-4	0.0	2.2263571746276183	1.0374582800299844E-4
76-77	1.0374582800299844E-4	9.950168049378486E-4	0.0	2.2289791146444218	1.0374582800299844E-4
78-79	1.0374582800299844E-4	0.0010185954022112575	0.0	2.2306249007341057	1.0374582800299844E-4
80-81	1.0374582800299844E-4	0.0010185954022112575	0.0	2.2313699844079453	1.1789298636704368E-4
82-83	1.0374582800299844E-4	0.0010327425605753027	0.0	2.2319405864619615	1.3204014473108892E-4
84-85	1.0374582800299844E-4	0.0010374582800299844	0.0	2.2321244995206944	1.3204014473108892E-4
86-87	1.0374582800299844E-4	0.001046889718939348	0.0	2.232176372434696	1.3204014473108892E-4
88	1.0374582800299844E-4	0.001103478352395529	0.0	2.2321858038736053	1.3204014473108892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8860	0.0	37.865623	1
GTATCAA	14745	0.0	31.836561	1
TCAACGC	15860	0.0	28.123808	4
ATCAACG	16540	0.0	26.993277	3
CAACGCA	16665	0.0	26.81632	5
AACGCAG	17220	0.0	25.952036	6
TATCAAC	17780	0.0	25.589033	2
ACGCAGA	20465	0.0	21.816214	7
GTACATG	35110	0.0	21.53291	1
CGCAGAG	21405	0.0	20.897884	8
TACATGG	35430	0.0	20.618345	2
ACATGGG	36260	0.0	19.560055	3
CATGGGG	26555	0.0	17.357405	4
GCAGAGT	26920	0.0	16.521841	9
GTGGTAT	5840	0.0	16.090933	1
AGAGTAC	22640	0.0	15.137754	10-11
GTGTAGC	5550	0.0	14.786526	1
CAGAGTA	25975	0.0	14.683843	10-11
ATGGGGG	14290	0.0	13.955404	5
AGGACCG	2190	0.0	13.785295	5
>>END_MODULE