FastQCFastQC Report
Fri 27 May 2016
SRR2140237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8361391
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78150.044.756481
GTATCAA133500.034.4082071
TCAACGC152500.028.7416784
ATCAACG155550.028.2626533
CAACGCA158250.027.6696555
AACGCAG160650.027.2290086
TATCAAC166350.026.9810682
ACGCAGA191750.022.7671437
GTACATG332300.022.0408381
TACATGG332800.021.5862712
TCGGTGG37400.021.33198494
ACATGGG340700.020.4939483
TGGTATC43300.019.5367092
GTGGTAT46750.019.5013071
CGCAGAG224050.019.4458088
CATGGGG256900.018.5117574
TATATAC97950.017.8546123
GCAGAGT244850.017.7043829
ATACAAC75450.017.4278586
TATACAA88850.017.0687085
Produced by FastQC (version 0.11.5)