##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8361391
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.638201107925703	31.0	31.0	34.0	29.0	34.0
2	31.717432542025602	33.0	31.0	34.0	28.0	34.0
3	31.851690227140438	34.0	31.0	34.0	29.0	34.0
4	35.33465149518782	37.0	35.0	37.0	32.0	37.0
5	35.399907025039255	37.0	35.0	37.0	33.0	37.0
6	35.41620216062136	37.0	35.0	37.0	32.0	37.0
7	35.34315964891488	37.0	35.0	37.0	32.0	37.0
8	35.33104312428399	37.0	35.0	37.0	32.0	37.0
9	36.890042458246484	39.0	37.0	39.0	33.0	39.0
10-11	36.86338828072985	39.0	37.0	39.0	32.0	39.0
12-13	36.797708180373334	39.0	37.0	39.0	32.0	39.0
14-15	38.12534296028018	40.0	38.0	41.0	32.5	41.0
16-17	38.135808264438296	40.0	38.0	41.0	32.5	41.0
18-19	38.13725419610206	40.0	38.0	41.0	32.0	41.0
20-21	38.204590373388385	40.0	38.0	41.0	32.0	41.0
22-23	38.18371547122027	40.0	38.0	41.0	32.0	41.0
24-25	38.1920335461116	40.0	38.0	41.0	32.0	41.0
26-27	38.17283891168916	40.0	38.0	41.0	32.0	41.0
28-29	38.14454618922237	40.0	38.0	41.0	32.0	41.0
30-31	38.0908275844875	40.0	38.0	41.0	32.0	41.0
32-33	38.052260558350824	40.0	38.0	41.0	32.0	41.0
34-35	37.98753904395802	40.0	37.5	41.0	32.0	41.0
36-37	37.919891842261535	40.0	37.0	41.0	32.0	41.0
38-39	37.82744246325987	40.0	37.0	41.0	32.0	41.0
40-41	37.74250419886303	40.0	37.0	41.0	32.0	41.0
42-43	37.64057132477845	40.0	37.0	41.0	31.0	41.0
44-45	37.50251458696597	40.0	36.0	41.0	31.0	41.0
46-47	37.39134052988935	40.0	36.0	41.0	31.0	41.0
48-49	37.27254204847972	39.5	36.0	41.0	31.0	41.0
50-51	37.11526908478515	39.0	35.5	41.0	31.0	41.0
52-53	36.97785002239711	39.0	35.0	41.0	30.0	41.0
54-55	36.81386713316296	39.0	35.0	40.0	30.0	41.0
56-57	36.616776072054094	38.5	35.0	40.0	30.0	41.0
58-59	36.76875339277332	39.0	35.0	40.0	30.5	41.0
60-61	36.83014968700637	39.0	35.0	40.0	31.0	41.0
62-63	36.65088729236733	38.0	35.0	40.0	31.0	41.0
64-65	36.418890525893474	38.0	35.0	40.0	30.5	41.0
66-67	36.111533043507244	37.0	34.5	40.0	30.0	41.0
68-69	35.81818809542128	37.0	34.0	39.5	30.0	41.0
70-71	35.561928366516085	36.0	34.0	39.0	30.0	41.0
72-73	35.30971131002934	36.0	34.0	39.0	30.0	41.0
74-75	35.037236501286316	35.0	34.0	38.5	30.0	40.0
76-77	34.784308641149735	35.0	34.0	37.0	30.0	39.5
78-79	34.4830931019777	35.0	34.0	37.0	29.0	39.0
80-81	34.20020713176863	35.0	34.0	36.5	29.0	39.0
82-83	33.948950967177	35.0	33.0	36.0	29.0	37.5
84-85	33.75035831180635	35.0	33.0	35.5	29.0	37.0
86-87	33.57970912680665	35.0	33.0	35.0	29.0	37.0
88-89	33.454918526955275	35.0	33.0	35.0	29.0	36.0
90-91	33.347715196647044	35.0	33.0	35.0	29.0	36.0
92-93	33.258408420337425	35.0	33.0	35.0	29.0	36.0
94-95	33.25535218796873	35.0	33.0	35.0	29.0	36.0
96-97	33.29403828115474	35.0	33.0	35.0	29.0	35.0
98-99	33.413133150872504	35.0	33.0	35.0	29.0	35.0
100	33.04868141757481	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	52.0
17	450.0
18	1483.0
19	4085.0
20	10610.0
21	21654.0
22	35304.0
23	50667.0
24	66437.0
25	81571.0
26	95038.0
27	104934.0
28	120861.0
29	146719.0
30	171126.0
31	199254.0
32	249670.0
33	322192.0
34	433194.0
35	606783.0
36	962005.0
37	1664140.0
38	2033310.0
39	927877.0
40	51972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00468558401347	19.632774020494914	14.095884285282198	24.266656110209414
2	14.717575102037447	20.426445791136906	41.17130750134756	23.684671605478083
3	18.51055643732006	25.248083721954877	29.55600330136457	26.685356539360495
4	12.900831930955029	17.356992395164873	37.66660355914465	32.07557211473546
5	14.161595929090124	37.40105394161294	34.625732565006665	13.811617564290271
6	31.31214650768036	36.27097453043399	18.563035743693842	13.853843218191805
7	27.880361054757515	31.94256792918786	22.210694368915412	17.966376647139214
8	25.379201305046173	36.073248586658444	20.40519578682492	18.14235432147047
9	25.911860837508975	17.298915933963617	20.749430328039917	36.03979290048749
10-11	24.88229973793225	26.550073178644094	27.913417480053397	20.654209603370255
12-13	25.370989874219934	24.140415568936255	27.87839280846028	22.610201748383535
14-15	22.653078334233705	25.425141683995328	25.457803721367345	26.463976260403626
16-17	22.625774475507015	28.535507486828287	26.009275138585004	22.82944289907969
18-19	22.763819840713992	28.15766831808214	26.963776737430678	22.114735103773185
20-21	23.890441353344766	27.14648589198757	26.722383851391058	22.240688903276613
22-23	23.640140351280657	27.00900947517274	26.508330692224458	22.84251948132215
24-25	23.22465007219008	27.23266366556556	26.689300312229214	22.853385950015152
26-27	23.014609352664603	27.237305422870918	26.736686467943365	23.01139875652111
28-29	23.089814976443545	27.237121164095402	26.70880519603039	22.964258663430666
30-31	23.43106169648031	26.942217517672006	26.617557807551997	23.009162978295688
32-33	23.174697871815958	27.0706322275932	26.629513200105208	23.12515670048563
34-35	23.340184221175612	27.068313409208255	26.667265197993235	22.924237171622895
36-37	23.340472310844696	26.781144210716313	26.686130073336845	23.192253405102147
38-39	22.984296789422938	26.96070314104896	26.91827581719659	23.136724252331515
40-41	23.17289069447151	26.663670672418178	27.041511920497896	23.12192671261242
42-43	22.7973658646654	26.758141502112576	26.99095556880747	23.453537064414558
44-45	22.701169167302858	26.65229895165805	27.179329969269983	23.467201911769106
46-47	22.67493220670315	26.60809893049128	27.24349883477733	23.47347002802824
48-49	22.603244889852235	26.69718626374885	27.25608088987263	23.443487956526283
50-51	22.441055160315056	26.668833584010095	27.34667331308347	23.543437942591382
52-53	22.60143303327638	26.577096690739676	27.337719943349644	23.4837503326343
54-55	22.504925693046417	26.513837695548638	27.34083880411149	23.640397807293457
56-57	22.442841315218082	26.70978300922404	27.22040125364524	23.626974421912642
58-59	22.31394747551283	26.63468760247762	27.297602847909396	23.753762074100152
60-61	22.350291579101572	26.476695083408337	27.256603220779517	23.916410116710573
62-63	22.383586609135673	26.458893976769932	27.30721263673408	23.85030677736032
64-65	22.596610721773345	26.431561550491047	27.100701184531538	23.871126543204067
66-67	22.394743687384707	26.709494453295417	26.719521520037397	24.176240339282472
68-69	22.46522165670682	26.774950627687943	26.6185985691635	24.141229146441738
70-71	22.59939570610394	26.731552604025698	26.415944111810553	24.253107578059808
72-73	22.737693433334293	26.67750579450539	25.957866716794037	24.62693405536628
74-75	22.719937285958352	26.59373783793236	25.916222042242993	24.770102833866297
76-77	23.040537820807792	26.437322284213938	26.01643433480688	24.50570556017139
78-79	23.084953847515997	26.21568178565931	26.219513103173593	24.4798512636511
80-81	23.266645648411792	26.075052182070767	26.226028282449125	24.432273887068312
82-83	23.35488430785755	26.11863331482927	26.201720738882607	24.324761638430576
84-85	23.322872302419224	26.00858028835936	26.29563616801103	24.37291124121038
86-87	23.003245158820356	26.311292711722057	26.519366007126006	24.166096122331577
88-89	23.194191577312733	26.369156683588717	26.230462133761414	24.20618960533714
90-91	23.162212236039547	26.660609387558125	26.46453040435152	23.712647972050803
92-93	22.95183533333736	26.632361111949898	26.823201959138316	23.592601595574425
94-95	22.656823388440543	26.73530056046534	27.089905842994167	23.517970208099946
96-97	22.764574010725052	26.61848893844857	27.313689014602794	23.303248036223582
98-99	24.277464331703225	28.709615516923026	27.27864515330956	19.73427499806419
100	23.541433390017207	29.912929054665067	21.018042648642336	25.52759490667539
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4089.0
1	2953.5
2	1931.0
3	2047.0
4	2008.5
5	2092.5
6	2342.5
7	2664.0
8	2938.5
9	3214.5
10	3643.0
11	3997.5
12	4338.5
13	4873.0
14	5541.0
15	6365.5
16	7410.5
17	8553.5
18	9913.0
19	11619.5
20	13784.5
21	16646.5
22	20485.0
23	25381.0
24	31474.0
25	39805.0
26	50263.5
27	61881.5
28	74417.0
29	88691.0
30	102242.0
31	115403.5
32	130299.0
33	145005.0
34	159451.0
35	173763.0
36	190487.5
37	207593.0
38	221823.5
39	235680.0
40	251833.0
41	265778.0
42	275854.5
43	287420.5
44	300378.5
45	317393.5
46	333766.0
47	350816.5
48	365266.0
49	371187.5
50	375527.0
51	377356.5
52	365229.0
53	344061.5
54	327910.5
55	312852.5
56	293773.5
57	269407.5
58	244753.5
59	215837.5
60	179900.0
61	151504.0
62	125357.5
63	100440.0
64	81966.0
65	63357.5
66	49231.0
67	37042.0
68	25800.5
69	19475.0
70	15119.0
71	11253.0
72	8554.5
73	6365.0
74	4738.0
75	3572.0
76	2767.0
77	2198.0
78	1803.0
79	1509.0
80	1242.5
81	1031.0
82	806.5
83	491.5
84	276.5
85	192.0
86	146.0
87	111.0
88	76.0
89	54.5
90	41.5
91	32.0
92	23.5
93	14.0
94	8.0
95	4.5
96	3.5
97	4.0
98	4.0
99	5.0
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.0331545313453228E-4
6	0.0
7	0.0
8	1.1959732537325427E-5
9	0.0
10-11	4.1859063880639E-5
12-13	1.25577191641917E-4
14-15	2.3919465074650855E-5
16-17	7.773826149261529E-5
18-19	9.448188704487088E-4
20-21	4.7870195178753295E-4
22-23	0.0
24-25	8.392445623996016E-5
26-27	1.2002214168469799E-4
28-29	0.0
30-31	0.0
32-33	4.22088672026429E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	2.4619391894865347E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.256077431488248E-4
76-77	6.686385709642496E-6
78-79	3.858379075996733E-4
80-81	2.7438967504579562E-5
82-83	2.0869241471940994E-5
84-85	3.9499387583155914E-4
86-87	7.158643998200031E-5
88-89	4.365351391142906E-5
90-91	2.2218576729818033E-5
92-93	4.982370711632009E-4
94-95	3.160190113954143E-4
96-97	3.6399434416092537E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3733.0
20-21	6897.0
22-23	7840.0
24-25	8655.0
26-27	10259.0
28-29	13190.0
30-31	15012.0
32-33	15558.0
34-35	17672.0
36-37	22227.0
38-39	24949.0
40-41	21435.0
42-43	16726.0
44-45	16585.0
46-47	16599.0
48-49	16404.0
50-51	16065.0
52-53	15664.0
54-55	15770.0
56-57	15169.0
58-59	15761.0
60-61	18238.0
62-63	45257.0
64-65	76883.0
66-67	80375.0
68-69	82165.0
70-71	81764.0
72-73	80906.0
74-75	83699.0
76-77	89609.0
78-79	96084.0
80-81	101244.0
82-83	99428.0
84-85	101946.0
86-87	110388.0
88-89	118875.0
90-91	126072.0
92-93	134059.0
94-95	150047.0
96-97	317717.0
98-99	558487.0
100-101	5495978.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.21053825122148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13257242015369	74.16769326796968
2	7.387523534318829	12.294396192684715
3	1.8823975946935394	4.699059511717622
4	0.6945133213171287	2.3116330915776726
5	0.33104672409947594	1.3773288049310504
6	0.17397877802151654	0.8686120658076104
7	0.10257575873491914	0.5974768867102038
8	0.07391300408219087	0.4920272682755066
9	0.04149778263503262	0.31077475463639315
>10	0.1731841107718631	2.3536986168230483
>50	0.005349497094509359	0.30239843641209285
>100	0.0013942840957860874	0.18396090828250014
>500	2.65949922716448E-5	0.015623664369277113
>1k	2.6594989298941836E-5	0.025316529802568863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3919465074650858E-5
2	0.0	2.3919465074650858E-5	0.0	0.0	2.3919465074650858E-5
3	0.0	2.3919465074650858E-5	0.0	0.0	2.3919465074650858E-5
4	0.0	3.587919761197628E-5	0.0	3.587919761197628E-5	3.587919761197628E-5
5	0.0	4.7838930149301716E-5	0.0	5.979866268662714E-5	3.587919761197628E-5
6	0.0	4.7838930149301716E-5	0.0	5.979866268662714E-5	3.587919761197628E-5
7	0.0	4.7838930149301716E-5	0.0	7.175839522395256E-5	3.587919761197628E-5
8	0.0	7.175839522395256E-5	0.0	7.175839522395256E-5	4.7838930149301716E-5
9	0.0	2.2723491820918313E-4	0.0	1.0763759283592885E-4	8.3718127761278E-5
10-11	1.1959732537325429E-5	2.2723491820918313E-4	0.0	1.734161217912187E-4	8.3718127761278E-5
12-13	1.1959732537325429E-5	2.2723491820918313E-4	0.0	1.9733558686586957E-4	8.3718127761278E-5
14-15	1.1959732537325429E-5	3.8271144119441373E-4	0.0	2.2723491820918313E-4	1.3753692417924242E-4
16-17	1.1959732537325429E-5	3.8271144119441373E-4	0.0	2.3919465074650857E-4	1.67436255522556E-4
18-19	1.1959732537325429E-5	4.1261077253772725E-4	0.0	3.169329122391239E-4	1.734161217912187E-4
20-21	2.3919465074650858E-5	4.4848997014970357E-4	0.0	4.4848997014970357E-4	1.8537585432854415E-4
22-23	2.3919465074650858E-5	4.963289002990054E-4	0.0	0.001046476597015975	2.1527518567185773E-4
24-25	2.3919465074650858E-5	5.621074292542951E-4	0.0	0.002469684768957701	2.3919465074650857E-4
26-27	2.3919465074650858E-5	5.979866268662715E-4	0.0	0.006242980384483874	2.3919465074650857E-4
28-29	2.3919465074650858E-5	5.979866268662715E-4	0.0	0.0282907473170433	2.3919465074650857E-4
30-31	2.3919465074650858E-5	6.458255570155731E-4	0.0	0.0924427526472569	2.4517451701517126E-4
32-33	2.3919465074650858E-5	6.757248883588867E-4	0.0	0.23835746946889577	2.9899331343313573E-4
34-35	2.3919465074650858E-5	7.654228823888275E-4	0.0	0.4368890295884979	3.2291277850778657E-4
36-37	2.3919465074650858E-5	8.491410101501055E-4	0.0	0.7301835304675981	3.34872511045112E-4
38-39	2.3919465074650858E-5	9.20899405374058E-4	0.0	1.1186715224775399	3.468322435824374E-4
40-41	2.3919465074650858E-5	0.0010524564632846377	0.0	1.5197172336516735	3.468322435824374E-4
42-43	2.989933134331357E-5	0.0011122551259712648	0.0	1.9841256078085572	3.6477184238842557E-4
44-45	4.1859063880639E-5	0.0012796913814938208	0.0	2.5529304872837546	3.8271144119441373E-4
46-47	4.7838930149301716E-5	0.0013992887068670751	0.0	3.1868800298897635	3.8271144119441373E-4
48-49	4.7838930149301716E-5	0.001405268573135738	0.0	3.8238254854963722	3.8271144119441373E-4
50-51	4.7838930149301716E-5	0.0015428054973149804	0.0	4.4572786992020825	3.9467117373173915E-4
52-53	4.7838930149301716E-5	0.0015547652298523056	0.0	5.070454186390757	4.0663090626906457E-4
54-55	4.7838930149301716E-5	0.0015667249623896311	0.0	5.693574191184219	4.245705050750527E-4
56-57	4.7838930149301716E-5	0.0016384833576135838	0.0	6.369376817804597	4.365302376123781E-4
58-59	4.7838930149301716E-5	0.001650443090150909	0.0	7.0637230097241	4.4251010388104083E-4
60-61	5.979866268662714E-5	0.001716221619106199	0.0	7.747801771260308	4.5446983641836625E-4
62-63	5.979866268662714E-5	0.0017580806829868378	0.0	8.394021999449613	4.7838930149301715E-4
64-65	5.979866268662714E-5	0.002009235066270672	0.0	8.55953871789993	4.7838930149301715E-4
66-67	5.979866268662714E-5	0.002033154531345323	0.0	8.563323973247993	4.7838930149301715E-4
68-69	5.979866268662714E-5	0.00209295319403195	0.0	8.566971691671878	4.8436916776167983E-4
70-71	5.979866268662714E-5	0.002134812257912589	0.0	8.569919765742327	5.142684991049935E-4
72-73	5.979866268662714E-5	0.002152751856718577	0.0	8.572760202219943	5.740671617916205E-4
74-75	5.979866268662714E-5	0.0021647115892559025	0.0	8.575122249396063	5.740671617916205E-4
76-77	5.979866268662714E-5	0.0022304901182111925	0.0	8.577257061653976	5.740671617916205E-4
78-79	5.979866268662714E-5	0.002260389449554506	0.0	8.579015142336964	5.740671617916205E-4
80-81	5.979866268662714E-5	0.0022663693158231687	0.0	8.579888202812187	6.039664931349341E-4
82-83	5.979866268662714E-5	0.002284308914629157	0.0	8.580402471311292	6.518054232842359E-4
84-85	5.979866268662714E-5	0.002302248513435145	0.0	8.580540008235472	6.577852895528985E-4
86-87	5.979866268662714E-5	0.002356067309853109	0.0	8.580605786764426	6.817047546275495E-4
88	5.979866268662714E-5	0.002427825705077062	0.0	8.580605786764426	7.056242197022003E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7815	0.0	44.75648	1
GTATCAA	13350	0.0	34.408207	1
TCAACGC	15250	0.0	28.741678	4
ATCAACG	15555	0.0	28.262653	3
CAACGCA	15825	0.0	27.669655	5
AACGCAG	16065	0.0	27.229008	6
TATCAAC	16635	0.0	26.981068	2
ACGCAGA	19175	0.0	22.767143	7
GTACATG	33230	0.0	22.040838	1
TACATGG	33280	0.0	21.586271	2
TCGGTGG	3740	0.0	21.331984	94
ACATGGG	34070	0.0	20.493948	3
TGGTATC	4330	0.0	19.536709	2
GTGGTAT	4675	0.0	19.501307	1
CGCAGAG	22405	0.0	19.445808	8
CATGGGG	25690	0.0	18.511757	4
TATATAC	9795	0.0	17.854612	3
GCAGAGT	24485	0.0	17.704382	9
ATACAAC	7545	0.0	17.427858	6
TATACAA	8885	0.0	17.068708	5
>>END_MODULE