FastQCFastQC Report
Fri 27 May 2016
SRR2140236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12809971
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110950.041.194671
GTATCAA195100.033.1396871
TCAACGC221100.027.9400524
ATCAACG228700.027.0663193
CAACGCA230950.026.6761175
AACGCAG235050.026.2640766
TATCAAC245200.025.7045922
ACGCAGA282100.021.8244427
GTACATG481350.021.5919861
TACATGG485150.020.9410652
ACATGGG495050.020.1091423
CGCAGAG315100.019.5652928
CATGGGG365400.018.0714154
GTGGTAT69800.017.1027071
GCAGAGT359700.016.8260359
TGGTATC66850.016.1715832
AGAGTAC304000.015.93403510-11
CAGAGTA341500.015.30880310-11
ATGGGGG195950.014.3784715
ATGGGGA186750.013.8575145
Produced by FastQC (version 0.11.5)