##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140236_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12809971
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40583222241487	31.0	31.0	34.0	28.0	34.0
2	31.49350658170889	31.0	31.0	34.0	28.0	34.0
3	31.639723150036797	33.0	31.0	34.0	28.0	34.0
4	35.14127432450862	37.0	35.0	37.0	32.0	37.0
5	35.21550813815269	37.0	35.0	37.0	32.0	37.0
6	35.21961681255953	37.0	35.0	37.0	32.0	37.0
7	35.14657121393952	37.0	35.0	37.0	32.0	37.0
8	35.12566367246264	37.0	35.0	37.0	32.0	37.0
9	36.659479947300426	39.0	37.0	39.0	32.0	39.0
10-11	36.63411732157707	39.0	37.0	39.0	32.0	39.0
12-13	36.572358594722814	39.0	37.0	39.0	32.0	39.0
14-15	37.84420917112146	40.0	38.0	41.0	32.0	41.0
16-17	37.84147711965936	40.0	38.0	41.0	32.0	41.0
18-19	37.835737372083045	40.0	37.5	41.0	32.0	41.0
20-21	37.91308733119739	40.0	38.0	41.0	32.0	41.0
22-23	37.88219043505258	40.0	37.5	41.0	32.0	41.0
24-25	37.88991874620385	40.0	37.0	41.0	32.0	41.0
26-27	37.873003568336216	40.0	37.0	41.0	32.0	41.0
28-29	37.84697419027904	40.0	37.0	41.0	32.0	41.0
30-31	37.79418008467788	40.0	37.0	41.0	32.0	41.0
32-33	37.75897425767293	40.0	37.0	41.0	31.0	41.0
34-35	37.696021666854435	40.0	37.0	41.0	31.0	41.0
36-37	37.62459402005048	40.0	37.0	41.0	31.0	41.0
38-39	37.53207641059798	40.0	37.0	41.0	31.0	41.0
40-41	37.45391536441896	40.0	36.5	41.0	31.0	41.0
42-43	37.34394308840505	40.0	36.0	41.0	30.5	41.0
44-45	37.188936603813524	39.5	36.0	41.0	30.0	41.0
46-47	37.06859318348252	39.0	36.0	41.0	30.0	41.0
48-49	36.94581347354392	39.0	35.0	41.0	30.0	41.0
50-51	36.79144610715278	39.0	35.0	40.0	30.0	41.0
52-53	36.65199023720329	39.0	35.0	40.0	30.0	41.0
54-55	36.48850810575657	38.5	35.0	40.0	29.5	41.0
56-57	36.295068492877206	38.0	34.0	40.0	29.0	41.0
58-59	36.451438016758246	38.0	34.5	40.0	29.5	41.0
60-61	36.52108452449845	38.0	35.0	40.0	30.0	41.0
62-63	36.35032195391986	38.0	35.0	40.0	30.0	41.0
64-65	36.135283452819806	37.5	34.0	40.0	30.0	41.0
66-67	35.852509516596	37.0	34.0	40.0	29.0	41.0
68-69	35.58207927078648	36.5	34.0	39.5	29.0	41.0
70-71	35.36604071840759	36.0	34.0	39.0	29.0	41.0
72-73	35.153770106647414	36.0	34.0	39.0	29.0	40.5
74-75	34.91196603629123	35.0	34.0	38.5	29.0	40.0
76-77	34.701103651122466	35.0	34.0	37.0	29.0	39.5
78-79	34.434506044108176	35.0	34.0	37.0	29.0	39.0
80-81	34.18414651479692	35.0	33.5	36.5	29.0	39.0
82-83	33.95414437022464	35.0	33.0	36.0	29.0	38.0
84-85	33.76730097651195	35.0	33.0	36.0	29.0	37.0
86-87	33.613621835342556	35.0	33.0	35.0	29.0	37.0
88-89	33.49819298776828	35.0	33.0	35.0	29.0	36.0
90-91	33.412884038510455	35.0	33.0	35.0	29.0	36.0
92-93	33.34042405274691	35.0	33.0	35.0	29.0	36.0
94-95	33.33494550056072	35.0	33.0	35.0	29.0	36.0
96-97	33.36835293030379	35.0	33.0	35.0	29.0	35.5
98-99	33.431963715758286	35.0	33.0	35.0	29.0	35.0
100	33.07139398124193	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	5.0
16	88.0
17	530.0
18	2303.0
19	7565.0
20	19633.0
21	39933.0
22	65546.0
23	92002.0
24	119793.0
25	146734.0
26	168684.0
27	182361.0
28	205354.0
29	242282.0
30	279679.0
31	321601.0
32	394290.0
33	501733.0
34	666113.0
35	920346.0
36	1407548.0
37	2352997.0
38	2920445.0
39	1674055.0
40	78346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63808801752947	19.874221417050826	14.25975125158363	24.22793931383607
2	14.558830773309323	20.516541372341905	41.41192825495077	23.512699599398
3	18.347278069560033	25.423578242292667	29.772198547522084	26.456945140625223
4	12.799310786886247	17.4451136540434	38.026463916272725	31.72911164279763
5	13.966717910674614	37.56586772800806	34.82386703526547	13.643547326051861
6	31.028235739175365	36.52153467014094	18.741830094697328	13.708399495986367
7	27.65124136502729	32.102391176373466	22.385398062181405	17.860969396417836
8	25.14629232485202	36.19404825991759	20.615797010578014	18.043862404652376
9	25.889598032657528	17.309164868523123	20.873692844425644	35.92754425439371
10-11	24.69536320057018	26.59924390908876	28.184181575792444	20.521211314548612
12-13	25.179724416771187	24.2465715836182	28.11489372780609	22.458810271804527
14-15	22.548297246140983	25.57055268554699	25.652957253333035	26.22819281497899
16-17	22.450250445668697	28.634116380030424	26.234862955118377	22.680770219182506
18-19	22.55801437675812	28.267756932521102	27.25165540683484	21.92257328388594
20-21	23.6346014757974	27.27528401061154	27.008643947276028	22.081470566315026
22-23	23.409742943793745	27.141985495122583	26.76953580834184	22.67873575274183
24-25	23.013516269351523	27.365636790431086	26.91614480034038	22.704702139877007
26-27	22.824991764869335	27.448833923133947	26.911254783087347	22.814919528909375
28-29	22.882133697208968	27.409390119025723	26.92750090730102	22.78097527646429
30-31	23.172988678454438	27.17965004529303	26.865469374565855	22.78189190168668
32-33	22.938608036373967	27.327775462700153	26.84201708511497	22.89159941581091
34-35	23.116622568315453	27.359207624760817	26.8553806593985	22.668789147525235
36-37	23.135398580659242	27.188818345809796	26.811283409614706	22.864499663916256
38-39	22.884563978027746	27.412583383229048	26.937492682161906	22.765359956581296
40-41	23.09802777691868	27.176234274236176	26.966966420641608	22.758771528203535
42-43	22.862127865403707	27.29850767788207	26.835625607423513	23.003738849290706
44-45	22.84890582373048	27.255892369977687	26.906199527891662	22.989002278400168
46-47	22.91481146263664	27.20014832237053	26.849627710186773	23.035412504806054
48-49	22.90117255257878	27.297033572587488	26.868955700510572	22.93283817432316
50-51	22.84416042818188	27.343850254576292	26.874153968818128	22.937835348423704
52-53	23.066864719966354	27.239095244121568	26.75843675951054	22.93560327640153
54-55	23.047832954423477	27.16299872978039	26.799006152927284	22.990162162868852
56-57	22.991393074500117	27.312186079158003	26.78140250841321	22.915018337928675
58-59	22.865313236953632	27.318072451025376	26.924536167441616	22.89207814457938
60-61	23.00327683281725	27.235910552008093	26.83590918667955	22.924903428495107
62-63	22.980876589902515	27.31137811083606	26.88946106598662	22.818284233274802
64-65	23.032036126711308	27.19009591746809	26.844350586896194	22.933517368924406
66-67	23.00691492310217	27.29498866569499	26.700164046022163	22.997932365180677
68-69	22.913143841535057	27.246881342877582	26.8180044128227	23.021970402764662
70-71	22.918457943673808	27.062810700832486	26.879974704833188	23.138756650660525
72-73	22.924091642824415	27.114953062132557	26.791211326484703	23.16974396855832
74-75	22.749660034803824	27.220566548859903	26.880015308898898	23.149758107437375
76-77	22.916995392968918	27.234219915730908	26.77830707853514	23.070477612765036
78-79	22.77847538340072	27.228090988665492	26.83644435408542	23.156989273848367
80-81	22.8409760026946	27.24057040493789	26.854649682116005	23.063803910251508
82-83	22.937760133409974	27.16858344114264	27.00772407081326	22.88593235463412
84-85	22.908803412749172	27.222189983565737	26.901629899523456	22.967376704161634
86-87	22.735800499352614	27.375386576858734	26.932017272704023	22.956795651084626
88-89	22.885876398835826	27.224167673550504	26.8587743140348	23.03118161357887
90-91	22.82931361426822	27.304675257783227	26.91271799709623	22.953293130852327
92-93	22.923805992632115	27.301733193488886	26.962015818278275	22.81244499560072
94-95	22.642452476715036	27.468000388494474	27.008713227805437	22.880833906985053
96-97	22.703679959312623	27.59545285525517	27.01133091190158	22.68953627353062
98-99	23.89941715342441	29.544321660840467	27.088782463256333	19.46747872247879
100	22.751746731546206	30.593217236994196	20.843749485861558	25.81128654559804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5038.0
1	3800.5
2	2693.5
3	2837.0
4	2772.5
5	2823.0
6	3223.5
7	3747.0
8	4194.5
9	4714.0
10	5303.5
11	5836.5
12	6401.0
13	7135.0
14	8136.0
15	9550.0
16	11398.5
17	13715.0
18	16615.5
19	20180.0
20	24751.5
21	30747.5
22	38688.0
23	49055.5
24	62525.0
25	79750.5
26	100915.0
27	123042.0
28	145328.5
29	172018.0
30	198363.5
31	222724.5
32	249983.0
33	277423.5
34	304056.0
35	328999.0
36	357393.5
37	384868.0
38	405044.0
39	423608.0
40	443610.0
41	460728.0
42	472609.5
43	487504.0
44	502090.5
45	519687.5
46	538067.0
47	555798.0
48	569308.0
49	570088.0
50	565933.0
51	562299.5
52	547187.0
53	520950.5
54	500300.5
55	480268.0
56	455437.5
57	423449.5
58	388453.0
59	348057.0
60	296353.0
61	249070.5
62	206211.0
63	167572.5
64	136979.0
65	107186.5
66	82980.0
67	63599.0
68	46962.0
69	35716.5
70	27684.5
71	21139.5
72	15962.5
73	11784.5
74	8727.0
75	6486.0
76	4952.0
77	3905.5
78	3140.5
79	2578.0
80	2069.0
81	1608.0
82	1239.5
83	806.0
84	502.0
85	361.5
86	286.0
87	221.5
88	143.5
89	93.5
90	70.0
91	56.5
92	46.0
93	26.5
94	12.0
95	8.5
96	10.0
97	9.5
98	5.5
99	13.5
100	48.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.4051554058943616E-4
6	0.0
7	0.0
8	2.3419256764906026E-5
9	0.0
10-11	2.7322466225723694E-5
12-13	8.977381759880643E-5
14-15	1.1709628382453013E-5
16-17	5.464493245144739E-5
18-19	0.0010070280408909591
20-21	5.11700060954805E-4
22-23	0.0
24-25	1.3308405385301038E-4
26-27	1.0581715297617405E-4
28-29	0.0
30-31	0.0
32-33	9.079587598815026E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	5.0671586427294653E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.8657685717886035E-4
76-77	1.935462674529742E-5
78-79	4.5048732935833754E-4
80-81	2.9746074125531108E-5
82-83	2.007855433204096E-5
84-85	3.3028987306350414E-4
86-87	7.718281490665665E-5
88-89	2.0859948237081447E-5
90-91	5.290356732457719E-6
92-93	5.371166685617933E-4
94-95	3.6578182042514766E-4
96-97	2.4558825811281273E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	6433.0
20-21	12337.0
22-23	13675.0
24-25	15260.0
26-27	19243.0
28-29	26600.0
30-31	39729.0
32-33	46972.0
34-35	60151.0
36-37	84852.0
38-39	94354.0
40-41	91069.0
42-43	97955.0
44-45	110284.0
46-47	111920.0
48-49	111043.0
50-51	107648.0
52-53	106305.0
54-55	113135.0
56-57	116435.0
58-59	116204.0
60-61	115309.0
62-63	112032.0
64-65	118115.0
66-67	122150.0
68-69	123525.0
70-71	120995.0
72-73	115947.0
74-75	117065.0
76-77	120716.0
78-79	125391.0
80-81	125597.0
82-83	121381.0
84-85	121790.0
86-87	127309.0
88-89	134718.0
90-91	141618.0
92-93	145917.0
94-95	172817.0
96-97	429279.0
98-99	755909.0
100-101	7840787.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.67419823390237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.77140396598236	76.0132165515132
2	7.058160607579255	11.952881809057734
3	1.7497361105597546	4.44472506847662
4	0.6324017204427891	2.141924345609345
5	0.2904074975217261	1.2295011006883076
6	0.164136871519185	0.833889479790482
7	0.09117739944679391	0.540426123664667
8	0.06186171851221047	0.4190473133114222
9	0.040490663961716154	0.30856630562250126
>10	0.13583449208675347	1.7741110207499937
>50	0.0032760602343557444	0.18798342510637475
>100	0.0010966694915379707	0.14092512355177966
>500	1.6222661604140208E-5	0.012802332857610515
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.806418921635341E-6
2	0.0	5.4644932451447395E-5	0.0	0.0	7.806418921635341E-6
3	0.0	5.4644932451447395E-5	0.0	0.0	7.806418921635341E-6
4	0.0	1.0928986490289479E-4	0.0	7.806418921635341E-6	7.806418921635341E-6
5	0.0	1.5612837843270684E-4	0.0	1.5612837843270683E-5	7.806418921635341E-6
6	0.0	1.5612837843270684E-4	0.0	1.5612837843270683E-5	7.806418921635341E-6
7	0.0	1.717412162759775E-4	0.0	3.1225675686541366E-5	7.806418921635341E-6
8	0.0	1.9516047304088353E-4	0.0	3.1225675686541366E-5	7.806418921635341E-6
9	0.0	2.9664391902214297E-4	0.0	4.683851352981205E-5	7.806418921635341E-6
10-11	7.806418921635341E-6	2.9664391902214297E-4	0.0	6.63545608339004E-5	7.806418921635341E-6
12-13	7.806418921635341E-6	3.005471284829607E-4	0.0	7.806418921635342E-5	7.806418921635341E-6
14-15	7.806418921635341E-6	4.8009476368057356E-4	0.0	8.196739867717109E-5	7.806418921635341E-6
16-17	7.806418921635341E-6	4.918043920630266E-4	0.0	8.587060813798875E-5	7.806418921635341E-6
18-19	7.806418921635341E-6	5.386429055928385E-4	0.0	8.587060813798875E-5	1.5612837843270683E-5
20-21	7.806418921635341E-6	5.854814191226506E-4	0.0	1.1319307436371245E-4	1.9516047304088355E-5
22-23	7.806418921635341E-6	6.323199326524627E-4	0.0	1.873540541192482E-4	2.7322466225723698E-5
24-25	7.806418921635341E-6	6.791584461822747E-4	0.0	2.927407095613253E-4	3.903209460817671E-5
26-27	7.806418921635341E-6	7.103841218688161E-4	0.0	7.181905407904514E-4	4.683851352981205E-5
28-29	7.806418921635341E-6	7.103841218688161E-4	0.0	0.0031147611497325013	4.683851352981205E-5
30-31	7.806418921635341E-6	7.845451016243518E-4	0.0	0.010042957942683867	5.4644932451447395E-5
32-33	7.806418921635341E-6	7.962547300068048E-4	0.0	0.02693604848910275	6.63545608339004E-5
34-35	7.806418921635341E-6	9.406734800570587E-4	0.0	0.045987613867353797	7.025777029471808E-5
36-37	7.806418921635341E-6	0.001092898649028948	0.0	0.06630381911090977	7.025777029471808E-5
38-39	7.806418921635341E-6	0.0011436403720195775	0.0	0.08668637891529965	7.025777029471808E-5
40-41	7.806418921635341E-6	0.0013309944261388259	0.0	0.10411811236731136	7.025777029471808E-5
42-43	1.5612837843270683E-5	0.001385639358590273	0.0	0.12020323855534099	7.025777029471808E-5
44-45	1.5612837843270683E-5	0.0015573805748662507	0.0	0.13333363518153163	7.025777029471808E-5
46-47	2.3419256764906023E-5	0.0016939929059948693	0.0	0.1457185188007061	7.025777029471808E-5
48-49	3.1225675686541366E-5	0.0017017993249165047	0.0	0.1556599932974087	8.587060813798875E-5
50-51	3.1225675686541366E-5	0.001963314358791288	0.0	0.16493011576685068	8.587060813798875E-5
52-53	3.1225675686541366E-5	0.0019750239871737414	0.0	0.1731502748913327	9.367702705962409E-5
54-55	3.1225675686541366E-5	0.002002346453399465	0.0	0.18103085479272357	1.0148344598125944E-4
56-57	3.1225675686541366E-5	0.0020374753385468242	0.0	0.18851330732911104	1.0538665544207712E-4
58-59	3.5128885147359034E-5	0.002049184966929277	0.0	0.19590598604789972	1.1319307436371247E-4
60-61	3.903209460817671E-5	0.002099926689919907	0.0	0.2023970233812395	1.1709628382453013E-4
62-63	5.4644932451447395E-5	0.002146765203449719	0.0	0.20826745041030928	1.1709628382453013E-4
64-65	5.4644932451447395E-5	0.0023809577710987794	0.0	0.21248681983745318	1.1709628382453013E-4
66-67	5.4644932451447395E-5	0.002384860980559597	0.0	0.2161597399400826	1.2490270274616546E-4
68-69	5.4644932451447395E-5	0.002439505913011044	0.0	0.21961017710344544	1.327091216678008E-4
70-71	5.4644932451447395E-5	0.002478538007619221	0.0	0.22252197136121543	1.327091216678008E-4
72-73	5.4644932451447395E-5	0.0025136668927665798	0.0	0.22500050936883464	1.5612837843270684E-4
74-75	5.4644932451447395E-5	0.0025566021968355746	0.0	0.22714337136282353	1.5612837843270684E-4
76-77	5.4644932451447395E-5	0.002646376014434381	0.0	0.22922378200543936	1.5612837843270684E-4
78-79	5.4644932451447395E-5	0.0027088273658074638	0.0	0.23083190430329625	1.5612837843270684E-4
80-81	5.4644932451447395E-5	0.0027205369941899166	0.0	0.23164377187114632	1.6393479735434219E-4
82-83	5.4644932451447395E-5	0.002724440203650734	0.0	0.23218241477673915	1.873540541192482E-4
84-85	5.4644932451447395E-5	0.0027439562509548225	0.0	0.23243222018223147	1.9125726358006588E-4
86-87	5.4644932451447395E-5	0.0028064076023279053	0.0	0.23249467153360456	1.990636825017012E-4
88	5.4644932451447395E-5	0.0029664391902214297	0.0	0.23249857474306537	2.1077331088415423E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11095	0.0	41.19467	1
GTATCAA	19510	0.0	33.139687	1
TCAACGC	22110	0.0	27.940052	4
ATCAACG	22870	0.0	27.066319	3
CAACGCA	23095	0.0	26.676117	5
AACGCAG	23505	0.0	26.264076	6
TATCAAC	24520	0.0	25.704592	2
ACGCAGA	28210	0.0	21.824442	7
GTACATG	48135	0.0	21.591986	1
TACATGG	48515	0.0	20.941065	2
ACATGGG	49505	0.0	20.109142	3
CGCAGAG	31510	0.0	19.565292	8
CATGGGG	36540	0.0	18.071415	4
GTGGTAT	6980	0.0	17.102707	1
GCAGAGT	35970	0.0	16.826035	9
TGGTATC	6685	0.0	16.171583	2
AGAGTAC	30400	0.0	15.934035	10-11
CAGAGTA	34150	0.0	15.308803	10-11
ATGGGGG	19595	0.0	14.378471	5
ATGGGGA	18675	0.0	13.857514	5
>>END_MODULE