FastQCFastQC Report
Fri 27 May 2016
SRR2140235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9903401
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87500.040.990561
GTATCAA153750.033.2790451
TCAACGC174450.028.0122574
ATCAACG180500.026.9807053
CAACGCA181100.026.9143985
AACGCAG184050.026.5057186
TATCAAC191000.026.1978322
ACGCAGA218150.022.266687
GTACATG364800.021.3597241
CGCAGAG230900.021.0552468
TACATGG365950.020.8927942
ACATGGG371700.019.9960293
CATGGGG278700.017.804034
GCAGAGT275100.017.4140019
AGAGTAC236500.015.934525510-11
CAGAGTA267950.015.37472610-11
ATGGGGG154450.014.8319135
GTGGTAT57500.014.3219751
GTATAGG28800.014.2245381
GAGTACA274450.013.3050112-13
Produced by FastQC (version 0.11.5)