##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9903401
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77268960430866	33.0	31.0	34.0	30.0	34.0
2	31.845027682914182	33.0	31.0	34.0	30.0	34.0
3	31.969527337123882	34.0	31.0	34.0	30.0	34.0
4	35.44034458465329	37.0	35.0	37.0	33.0	37.0
5	35.51089105651685	37.0	35.0	37.0	33.0	37.0
6	35.533049201986266	37.0	35.0	37.0	33.0	37.0
7	35.46404704807975	37.0	35.0	37.0	33.0	37.0
8	35.45046868242536	37.0	35.0	37.0	33.0	37.0
9	37.02393803906355	39.0	37.0	39.0	33.0	39.0
10-11	36.99971368421818	39.0	37.0	39.0	33.0	39.0
12-13	36.93930473985654	39.0	37.0	39.0	33.0	39.0
14-15	38.29753924939523	40.0	38.0	41.0	33.0	41.0
16-17	38.3131335386702	40.0	38.0	41.0	33.0	41.0
18-19	38.324962454817296	40.0	38.0	41.0	33.0	41.0
20-21	38.39590696408548	40.0	38.0	41.0	33.0	41.0
22-23	38.38207885469251	40.0	38.0	41.0	33.0	41.0
24-25	38.395405529547716	40.0	38.0	41.0	33.0	41.0
26-27	38.38196681023618	40.0	38.0	41.0	33.0	41.0
28-29	38.36058938051316	40.0	38.0	41.0	33.0	41.0
30-31	38.31202077246992	40.0	38.0	41.0	33.0	41.0
32-33	38.27374986321614	40.0	38.0	41.0	33.0	41.0
34-35	38.2120717032402	40.0	38.0	41.0	33.0	41.0
36-37	38.149987902781724	40.0	38.0	41.0	33.0	41.0
38-39	38.06490426486127	40.0	38.0	41.0	32.5	41.0
40-41	37.99098025086653	40.0	37.5	41.0	32.0	41.0
42-43	37.89359847920766	40.0	37.0	41.0	32.0	41.0
44-45	37.751750293185765	40.0	37.0	41.0	32.0	41.0
46-47	37.63983808272886	40.0	37.0	41.0	31.5	41.0
48-49	37.53179742045621	40.0	36.0	41.0	31.0	41.0
50-51	37.3828727100789	40.0	36.0	41.0	31.0	41.0
52-53	37.248804453874	39.0	36.0	41.0	31.0	41.0
54-55	37.09464915438221	39.0	35.5	41.0	31.0	41.0
56-57	36.90637369316872	39.0	35.0	40.5	30.0	41.0
58-59	37.0458489837777	39.0	35.0	40.5	31.0	41.0
60-61	37.09935618721275	39.0	35.0	41.0	31.0	41.0
62-63	36.92698750015094	39.0	35.0	40.5	31.0	41.0
64-65	36.707726566074406	38.0	35.0	40.0	31.0	41.0
66-67	36.42440655621144	37.5	35.0	40.0	31.0	41.0
68-69	36.14396064796991	37.0	35.0	40.0	30.5	41.0
70-71	35.90611362715478	36.5	34.0	39.0	30.0	41.0
72-73	35.67054163819948	36.0	34.0	39.0	30.0	41.0
74-75	35.40792485522102	36.0	34.0	39.0	30.0	40.5
76-77	35.17128284582162	35.0	34.0	37.5	30.0	40.0
78-79	34.88498143776667	35.0	34.0	37.0	30.0	39.0
80-81	34.61540857895959	35.0	34.0	37.0	30.0	39.0
82-83	34.370224197050455	35.0	34.0	36.0	30.0	38.5
84-85	34.16800114022875	35.0	34.0	36.0	30.0	37.0
86-87	33.99752170383587	35.0	34.0	36.0	30.0	37.0
88-89	33.86731265868345	35.0	34.0	35.0	30.0	36.5
90-91	33.77025452376331	35.0	34.0	35.0	30.0	36.0
92-93	33.689787202067926	35.0	34.0	35.0	30.0	36.0
94-95	33.66633696523618	35.0	34.0	35.0	30.0	36.0
96-97	33.67494962190899	35.0	34.0	35.0	30.0	36.0
98-99	33.7089228047697	35.0	34.0	35.0	30.0	35.0
100	33.374256496777065	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	4.0
16	62.0
17	418.0
18	1492.0
19	4344.0
20	11126.0
21	22592.0
22	36885.0
23	52863.0
24	69147.0
25	85682.0
26	98909.0
27	106036.0
28	122458.0
29	150029.0
30	175484.0
31	202308.0
32	255011.0
33	331787.0
34	451141.0
35	635242.0
36	990886.0
37	1798109.0
38	2548994.0
39	1652501.0
40	99889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49679488894775	20.095338964866716	14.340750212982389	24.067115933203148
2	14.440594700749774	20.72769748493472	41.65019673544472	23.181511078870784
3	18.01908253538355	25.856612289051007	30.177016966191715	25.94728820937373
4	12.63851680851861	17.706058756986614	38.605081224116844	31.050343210377928
5	13.663430601095545	37.89643014953272	35.085370818701975	13.354768430669758
6	30.40762461299911	37.10024465332667	19.05970484281107	13.432425890863147
7	27.19017436535186	32.46242376735023	22.823007974735145	17.524393892562767
8	24.829349028682167	36.356166936994676	21.0479511028585	17.766532931464653
9	25.83272150648045	17.418965464490434	21.119835498936173	35.628477530092944
10-11	24.372353150047115	26.776305449457027	28.644355910311404	20.206985490184458
12-13	24.93935663047132	24.465791565737433	28.503898311194618	22.090953492596626
14-15	22.371176154445987	25.81457020179186	25.965952593541765	25.84830105022039
16-17	22.237714108459	28.872395437845945	26.604101907710003	22.285788545985053
18-19	22.339451861888154	28.49533494215619	27.637293279911447	21.527919916044205
20-21	23.40942337434616	27.575782113520354	27.256596640376774	21.758197871756714
22-23	23.12447579709172	27.396188615182293	27.024924559265155	22.45441102846083
24-25	22.74540283345498	27.627311686270318	27.167694948450325	22.459590531824375
26-27	22.5916029805667	27.733973336585137	27.137046707337518	22.53737697551064
28-29	22.627051273677203	27.724412352613992	27.181584756362504	22.466951617346304
30-31	22.915764936164088	27.56082144709372	27.031844055734926	22.491569561007267
32-33	22.673774992054526	27.71675907754775	27.03045546826719	22.57901046213054
34-35	22.814923131964022	27.7141086625518	27.07587144921187	22.395096756272306
36-37	22.804137189190257	27.494620600599813	27.093300327887203	22.60794188232273
38-39	22.601113984883632	27.68415309412674	27.223304765181773	22.491428155807856
40-41	22.849372964082676	27.4593328026352	27.184928256585657	22.506365976696465
42-43	22.640125226124194	27.52860392526837	27.090576454415732	22.740694394191703
44-45	22.639997853593893	27.55239924510982	27.131458678076836	22.67614422321945
46-47	22.7142062210328	27.464470881272845	27.08659888553181	22.734724012162545
48-49	22.61161485845465	27.50961959331536	27.15430787570999	22.72445767252
50-51	22.581532592592353	27.5596556874883	27.155892207761195	22.702919512158154
52-53	22.74673969973988	27.494866925172644	27.053405984956186	22.704987390131294
54-55	22.79325583104024	27.353809632208886	27.092122352645337	22.760812184105532
56-57	22.745188793196792	27.48489300980166	27.07509258308634	22.694825613915206
58-59	22.64796333993171	27.515582688739453	27.17250533424264	22.663948637086197
60-61	22.732656988734153	27.47265693031766	27.131590532702837	22.663095548245348
62-63	22.758573526772498	27.546016898444904	27.064224111347052	22.631185463435553
64-65	22.732943282459775	27.4638766876212	27.06170248484993	22.741477545069092
66-67	22.8252756818489	27.547080313993067	26.901579332668696	22.726064671489336
68-69	22.696605247270256	27.482035901116888	27.070771187545617	22.75058766406724
70-71	22.695846953775394	27.273966479757703	27.135664047917267	22.894522518549635
72-73	22.77412703523765	27.368331036615682	27.01341230910166	22.84412961904501
74-75	22.556369672626445	27.494136024677296	27.1040416127103	22.845452689985965
76-77	22.73057444285712	27.398469599627322	27.04861408012912	22.82234187738644
78-79	22.598122036455212	27.468461090817662	27.027446087183087	22.905970785544035
80-81	22.676444654221463	27.496274896530608	27.00024036237355	22.82704008687438
82-83	22.717339821784172	27.431872890468412	27.15987122720731	22.690916060540108
84-85	22.746435027127088	27.477215626644757	27.05377705391518	22.722572292312975
86-87	22.561362661177185	27.540905665494787	27.153386371123588	22.744345302204437
88-89	22.660798889762326	27.479367806522646	27.065188464559302	22.79464483915572
90-91	22.62164858856363	27.55986027975399	27.120446937224795	22.698044194457584
92-93	22.70151002713867	27.532798061741698	27.140645284976898	22.62504662614273
94-95	22.514361831812916	27.633686798910084	27.17748453059004	22.674466838686957
96-97	22.596533050617364	27.724393134739145	27.198860702857125	22.480213111786366
98-99	23.672949367366815	29.71939536713233	27.32187566996342	19.285779595537438
100	22.602284662809833	30.73261516392965	21.066240519200676	25.59885965405984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3864.0
1	2992.0
2	2340.0
3	2543.0
4	2537.5
5	2705.0
6	3090.0
7	3552.0
8	3929.5
9	4369.0
10	4963.0
11	5513.5
12	6112.5
13	6892.0
14	7800.0
15	8931.5
16	10442.5
17	12300.5
18	14509.0
19	17241.0
20	20834.5
21	25785.5
22	32413.0
23	40696.0
24	51214.0
25	65123.0
26	83056.5
27	101893.0
28	120739.5
29	142895.5
30	164644.5
31	185351.0
32	208111.5
33	230472.5
34	252518.0
35	273621.0
36	296492.5
37	318463.5
38	333172.0
39	344706.5
40	359878.5
41	373466.5
42	381860.0
43	391398.0
44	401091.0
45	412727.5
46	421217.0
47	425489.0
48	429466.0
49	429158.5
50	425928.5
51	419793.5
52	407406.5
53	388372.5
54	367796.0
55	348843.5
56	327800.5
57	300551.5
58	271644.0
59	241813.5
60	207570.0
61	173630.0
62	141677.5
63	113088.5
64	89729.0
65	70544.5
66	54996.5
67	42495.5
68	32489.0
69	25115.5
70	19338.0
71	14291.0
72	10547.5
73	7753.0
74	5711.5
75	4209.0
76	3160.0
77	2431.5
78	1860.0
79	1438.5
80	1075.5
81	787.0
82	600.5
83	419.0
84	305.0
85	228.5
86	171.0
87	132.0
88	79.5
89	47.0
90	38.0
91	30.5
92	22.0
93	17.0
94	13.0
95	10.5
96	10.5
97	8.0
98	4.5
99	7.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.7165820105638456E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.5146311857916285E-5
12-13	1.0097541238610857E-4
14-15	3.5341394335138E-5
16-17	9.087787114749772E-5
18-19	9.491688764294206E-4
20-21	4.7483330446447867E-4
22-23	0.0
24-25	1.0624040990585583E-4
26-27	1.7724195116447454E-4
28-29	0.0
30-31	0.0
32-33	9.176672978858169E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	4.906722389586888E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	2.103497392312843E-4
76-77	1.877914405788133E-5
78-79	4.625567004221115E-4
80-81	3.208252755391838E-5
82-83	3.2485557733170776E-5
84-85	5.261876350904537E-4
86-87	1.132317041045094E-4
88-89	2.6997309920546243E-5
90-91	0.0
92-93	6.668275388104046E-4
94-95	4.1622190299334805E-4
96-97	3.313742153544836E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3539.0
20-21	6701.0
22-23	7820.0
24-25	9029.0
26-27	12588.0
28-29	18788.0
30-31	29484.0
32-33	34598.0
34-35	45270.0
36-37	65329.0
38-39	72776.0
40-41	70755.0
42-43	76020.0
44-45	85586.0
46-47	87306.0
48-49	85894.0
50-51	83269.0
52-53	83208.0
54-55	90177.0
56-57	91981.0
58-59	90222.0
60-61	89423.0
62-63	87337.0
64-65	91850.0
66-67	96470.0
68-69	96259.0
70-71	94782.0
72-73	92192.0
74-75	93392.0
76-77	95648.0
78-79	98501.0
80-81	97672.0
82-83	94121.0
84-85	94607.0
86-87	97274.0
88-89	102309.0
90-91	106232.0
92-93	108113.0
94-95	126094.0
96-97	321033.0
98-99	575461.0
100-101	6094291.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.50841743963046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.42339777518474	77.3196164327025
2	6.692694379312113	11.445634095641774
3	1.5699902423554397	4.027421430584266
4	0.5857126980189186	2.0033346352757566
5	0.26865005917385604	1.1485920702509753
6	0.14967378529708442	0.7679021107771631
7	0.08557151926912553	0.5121959633425438
8	0.05117059824265686	0.3500413500134979
9	0.03675712197696224	0.28287389968969523
>10	0.131974695615259	1.817712761456875
>50	0.003372444853173067	0.1970429239180337
>100	0.0010346807006542474	0.12763232634705432
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.058524743166514E-5	0.0	0.0	0.0
3	0.0	6.058524743166514E-5	0.0	0.0	0.0
4	0.0	1.2117049486333028E-4	0.0	1.0097541238610857E-5	1.0097541238610857E-5
5	0.0	1.2117049486333028E-4	0.0	2.0195082477221713E-5	1.0097541238610857E-5
6	0.0	1.3126803610194113E-4	0.0	2.0195082477221713E-5	2.0195082477221713E-5
7	0.0	1.615606598177737E-4	0.0	2.0195082477221713E-5	2.0195082477221713E-5
8	0.0	1.9185328353360628E-4	0.0	2.0195082477221713E-5	2.0195082477221713E-5
9	0.0	3.9380410830582344E-4	0.0	2.0195082477221713E-5	2.0195082477221713E-5
10-11	2.0195082477221713E-5	3.9380410830582344E-4	0.0	3.029262371583257E-5	2.0195082477221713E-5
12-13	4.0390164954443427E-5	3.9380410830582344E-4	0.0	3.029262371583257E-5	2.524385309652714E-5
14-15	4.0390164954443427E-5	5.3012091502707E-4	0.0	5.048770619305428E-5	4.0390164954443427E-5
16-17	4.543893557374885E-5	5.452672268849863E-4	0.0	7.0682788670276E-5	4.0390164954443427E-5
18-19	5.048770619305428E-5	5.705110799815135E-4	0.0	1.1107295362471943E-4	4.543893557374885E-5
20-21	5.048770619305428E-5	6.310963274131786E-4	0.0	1.5146311857916286E-4	5.5536476812359715E-5
22-23	5.048770619305428E-5	7.472180516572034E-4	0.0	2.5243853096527144E-4	9.087787114749772E-5
24-25	6.563401805097056E-5	8.784860877591445E-4	0.0	4.745844382147103E-4	1.1107295362471943E-4
26-27	7.0682788670276E-5	9.18876252713588E-4	0.0	0.0011814123249174703	1.1107295362471943E-4
28-29	7.0682788670276E-5	9.239250233328935E-4	0.0	0.005639476781764163	1.1107295362471943E-4
30-31	7.0682788670276E-5	0.001095583224389278	0.0	0.018816768098151332	1.1107295362471943E-4
32-33	7.0682788670276E-5	0.0011056807656278887	0.0	0.048978123777881966	1.1107295362471943E-4
34-35	9.592664176680314E-5	0.0013126803610194115	0.0	0.0808207200738413	1.2117049486333028E-4
36-37	1.2117049486333028E-4	0.0015398750388881557	0.0	0.1158743344836789	1.2117049486333028E-4
38-39	1.2117049486333028E-4	0.001580265203842599	0.0	0.147035346746032	1.2117049486333028E-4
40-41	1.2117049486333028E-4	0.0017872647992341216	0.0	0.1734555633968573	1.2117049486333028E-4
42-43	1.2117049486333028E-4	0.001837752505427176	0.0	0.19737663859112642	1.2117049486333028E-4
44-45	1.3126803610194113E-4	0.0021154348894889745	0.0	0.21434050787199266	1.2117049486333028E-4
46-47	1.4641434795985742E-4	0.0023426295673577187	0.0	0.2281337492039351	1.3126803610194113E-4
48-49	1.5146311857916286E-4	0.0023426295673577187	0.0	0.23803943715901232	1.41365577340552E-4
50-51	1.5146311857916286E-4	0.002605165639561601	0.0	0.24603668981999216	1.41365577340552E-4
52-53	1.565118891984683E-4	0.002640507033896739	0.0	0.25189326373838644	1.565118891984683E-4
54-55	1.615606598177737E-4	0.0026556533457546554	0.0	0.25713893641184477	1.7670697167569E-4
56-57	1.615606598177737E-4	0.0027061410519477096	0.0	0.26232402383787146	1.8680451291430087E-4
58-59	1.615606598177737E-4	0.002721287363805626	0.0	0.2674687210989437	1.9185328353360628E-4
60-61	1.615606598177737E-4	0.0027515799875214584	0.0	0.27197222449136416	1.9185328353360628E-4
62-63	1.615606598177737E-4	0.002807116464333818	0.0	0.27635455738892123	2.12048366010828E-4
64-65	1.615606598177737E-4	0.0031201402427307547	0.0	0.2799492820698667	2.12048366010828E-4
66-67	1.615606598177737E-4	0.0031201402427307547	0.0	0.28339759240285234	2.12048366010828E-4
68-69	1.7165820105638456E-4	0.0032009205726396418	0.0	0.2870629998724681	2.12048366010828E-4
70-71	1.7165820105638456E-4	0.0032463595082133905	0.0	0.29024372536263043	2.12048366010828E-4
72-73	1.7165820105638456E-4	0.003261505820071307	0.0	0.29300035412077124	2.322434484880497E-4
74-75	1.7165820105638456E-4	0.0033725787736960263	0.0	0.2953126910644131	2.4234098972666056E-4
76-77	1.7165820105638456E-4	0.0035644320572296327	0.0	0.297625028008055	2.4234098972666056E-4
78-79	1.7165820105638456E-4	0.0036452123871385193	0.0	0.29932646370676097	2.4234098972666056E-4
80-81	1.7165820105638456E-4	0.0036502611577578247	0.0	0.3001494133177077	2.4738976034596597E-4
82-83	1.7165820105638456E-4	0.0036603586989964357	0.0	0.3007704121038823	2.5243853096527144E-4
84-85	1.7165820105638456E-4	0.003685602552092963	0.0	0.301012753093609	2.5243853096527144E-4
86-87	1.7165820105638456E-4	0.0038118218175755983	0.0	0.30113392358847224	2.5748730158457685E-4
88	1.7165820105638456E-4	0.004018821412967121	0.0	0.3011490699003302	2.6253607220388226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8750	0.0	40.99056	1
GTATCAA	15375	0.0	33.279045	1
TCAACGC	17445	0.0	28.012257	4
ATCAACG	18050	0.0	26.980705	3
CAACGCA	18110	0.0	26.914398	5
AACGCAG	18405	0.0	26.505718	6
TATCAAC	19100	0.0	26.197832	2
ACGCAGA	21815	0.0	22.26668	7
GTACATG	36480	0.0	21.359724	1
CGCAGAG	23090	0.0	21.055246	8
TACATGG	36595	0.0	20.892794	2
ACATGGG	37170	0.0	19.996029	3
CATGGGG	27870	0.0	17.80403	4
GCAGAGT	27510	0.0	17.414001	9
AGAGTAC	23650	0.0	15.9345255	10-11
CAGAGTA	26795	0.0	15.374726	10-11
ATGGGGG	15445	0.0	14.831913	5
GTGGTAT	5750	0.0	14.321975	1
GTATAGG	2880	0.0	14.224538	1
GAGTACA	27445	0.0	13.30501	12-13
>>END_MODULE