FastQCFastQC Report
Fri 27 May 2016
SRR2140234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12136593
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101350.037.267631
GTATCAA170100.032.052041
TCAACGC190500.027.4767784
ATCAACG195200.026.8580993
CAACGCA198550.026.3416715
AACGCAG202750.025.8373056
TATCAAC212250.025.0557142
ACGCAGA240200.021.7566667
CGCAGAG249300.020.8953138
GTACATG404250.020.8931941
TACATGG407400.020.3103162
ACATGGG418350.019.2882443
CATGGGG303100.017.3521964
GCAGAGT314500.016.3903489
AGAGTAC259750.015.60524510-11
TATACCG9650.015.1876825
CAGAGTA297950.014.65857310-11
GTGGTAT65150.013.7546121
ATGGGGG173400.013.7408615
GTATAAG50850.013.258181
Produced by FastQC (version 0.11.5)