##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12136593
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77832609200951	33.0	31.0	34.0	30.0	34.0
2	31.86119514760032	33.0	31.0	34.0	30.0	34.0
3	31.987698442223447	34.0	31.0	34.0	30.0	34.0
4	35.46530150594982	37.0	35.0	37.0	33.0	37.0
5	35.53806789104652	37.0	35.0	37.0	33.0	37.0
6	35.557631948274114	37.0	35.0	37.0	33.0	37.0
7	35.49081006506521	37.0	35.0	37.0	33.0	37.0
8	35.4762018467621	37.0	35.0	37.0	33.0	37.0
9	37.057603398251885	39.0	37.0	39.0	33.0	39.0
10-11	37.03689878205523	39.0	37.0	39.0	33.0	39.0
12-13	36.98004320487636	39.0	37.0	39.0	33.0	39.0
14-15	38.338564167060724	40.0	38.0	41.0	33.0	41.0
16-17	38.34724423897217	40.0	38.0	41.0	33.0	41.0
18-19	38.355520202415946	40.0	38.0	41.0	33.0	41.0
20-21	38.42147445633062	40.0	38.0	41.0	33.0	41.0
22-23	38.40385295609612	40.0	38.0	41.0	33.0	41.0
24-25	38.414548621405174	40.0	38.0	41.0	33.0	41.0
26-27	38.40148348052645	40.0	38.0	41.0	33.0	41.0
28-29	38.375960208254895	40.0	38.0	41.0	33.0	41.0
30-31	38.32631784036643	40.0	38.0	41.0	33.0	41.0
32-33	38.28774984976456	40.0	38.0	41.0	33.0	41.0
34-35	38.22677696016093	40.0	38.0	41.0	33.0	41.0
36-37	38.16471156065051	40.0	38.0	41.0	33.0	41.0
38-39	38.083269919149586	40.0	38.0	41.0	33.0	41.0
40-41	38.0092043843544	40.0	37.5	41.0	32.0	41.0
42-43	37.91141745070932	40.0	37.0	41.0	32.0	41.0
44-45	37.77058544200554	40.0	37.0	41.0	32.0	41.0
46-47	37.66057004726436	40.0	37.0	41.0	32.0	41.0
48-49	37.55203382310161	40.0	36.5	41.0	31.0	41.0
50-51	37.40958555587996	40.0	36.0	41.0	31.0	41.0
52-53	37.280218416560345	39.0	36.0	41.0	31.0	41.0
54-55	37.1287311508964	39.0	35.5	41.0	31.0	41.0
56-57	36.939837613014035	39.0	35.0	40.5	30.5	41.0
58-59	37.08699724358125	39.0	35.0	40.5	31.0	41.0
60-61	37.14564478058075	39.0	35.0	41.0	31.0	41.0
62-63	36.9750935885677	39.0	35.0	40.5	31.0	41.0
64-65	36.760522295044694	38.0	35.0	40.0	31.0	41.0
66-67	36.48104710881006	38.0	35.0	40.0	31.0	41.0
68-69	36.200877091420544	37.0	35.0	40.0	31.0	41.0
70-71	35.96446002593865	37.0	34.0	39.5	30.5	41.0
72-73	35.72911648287675	36.0	34.0	39.0	30.0	41.0
74-75	35.465798532263165	36.0	34.0	39.0	30.0	40.5
76-77	35.22929154189099	35.5	34.0	38.5	30.0	40.0
78-79	34.936645885322534	35.0	34.0	37.0	30.0	39.0
80-81	34.66213387308099	35.0	34.0	37.0	30.0	39.0
82-83	34.41058736467336	35.0	34.0	36.0	30.0	39.0
84-85	34.2008641186426	35.0	34.0	36.0	30.0	37.0
86-87	34.02413240244597	35.0	34.0	36.0	30.0	37.0
88-89	33.88977237211908	35.0	34.0	35.0	30.0	37.0
90-91	33.78686210214376	35.0	34.0	35.0	30.0	36.0
92-93	33.701518328738175	35.0	34.0	35.0	30.0	36.0
94-95	33.677596135846144	35.0	34.0	35.0	30.0	36.0
96-97	33.68123885311732	35.0	34.0	35.0	30.0	36.0
98-99	33.71558531073126	35.0	34.0	35.0	30.0	35.5
100	33.37569444490901	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	80.0
17	453.0
18	1675.0
19	4932.0
20	13010.0
21	26466.0
22	42996.0
23	61475.0
24	80970.0
25	101452.0
26	118051.0
27	126286.0
28	146843.0
29	180617.0
30	212241.0
31	248492.0
32	314509.0
33	408419.0
34	554373.0
35	777006.0
36	1208391.0
37	2197511.0
38	3158929.0
39	2035206.0
40	116202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3032636094825	20.315816802952856	14.30021588430954	24.080703703255107
2	14.284865612614677	20.861348815108162	41.84296202402108	23.010823548256088
3	17.84965517093636	26.045324252036796	30.416130787281077	25.688889789745772
4	12.496324133140165	17.91036413596468	39.025482686945175	30.567829043949978
5	13.457474932581864	38.02411735571769	35.402231816548515	13.116175895151933
6	30.05212418345082	37.49099932740597	19.199490334725734	13.257386154417471
7	26.783282590097567	32.64189546440257	23.16023945105517	17.414582494444694
8	24.51038149754066	36.587149011847806	21.31620639467818	17.586263095933354
9	25.60536552556389	17.483481566861474	21.364636681810126	35.54651622576451
10-11	24.12417198196544	26.865115656696386	29.012473976929183	19.998238384408992
12-13	24.710510509941415	24.530620465954144	28.847936204106254	21.910932819998187
14-15	22.141933312882774	26.022138678691135	26.20308740834635	25.632840600079742
16-17	22.012270177243494	28.997375784827707	26.87541491298085	22.11493912494796
18-19	22.089092762856826	28.671198076146332	27.87295964214977	21.366749518847072
20-21	23.114671054448422	27.778079584325827	27.521028336887003	21.58622102433875
22-23	22.854578987738	27.60600747703131	27.308408548329705	22.231004986900988
24-25	22.459793855282644	27.83022201089334	27.392915694636372	22.317068439187636
26-27	22.385755754686755	27.944230502685212	27.355489129525456	22.31452461310257
28-29	22.3961363847896	27.94405617547597	27.38108938229728	22.27871805743715
30-31	22.672034436627673	27.779817806696034	27.269796760478293	22.278350996198
32-33	22.430721158688197	27.945270664108474	27.253503736350286	22.370504440853047
34-35	22.5846653729485	27.88711909263154	27.293433841540033	22.234781692879928
36-37	22.529111675246032	27.732518746198963	27.36273685452762	22.375632724027387
38-39	22.37148174484599	27.863539673738245	27.483230293063198	22.281748288352563
40-41	22.620321296381505	27.661919167174165	27.420049146404335	22.29771039004
42-43	22.408766158103425	27.743361207303586	27.34900938558791	22.49886324900508
44-45	22.471058208172963	27.752752678260855	27.351425002721086	22.424764110845093
46-47	22.46428467577658	27.699650521153735	27.293943556493566	22.542121246576123
48-49	22.36102900518841	27.723898183667384	27.42378331176456	22.491289499379647
50-51	22.343728478962262	27.778195715206966	27.36816141631109	22.509914389519682
52-53	22.51296408596266	27.72719060499694	27.248303672796485	22.511541636243912
54-55	22.52358624201912	27.568776698397922	27.34257722740517	22.565059832177788
56-57	22.477426712747828	27.663171785370167	27.338614084622144	22.520787417259857
58-59	22.456366925786746	27.72108907003291	27.366771401064092	22.45577260311625
60-61	22.553381151460986	27.633777174401253	27.34983047256465	22.463011201573117
62-63	22.562133885439142	27.750905951408022	27.286571324280455	22.400388838872377
64-65	22.524691682352184	27.684037866422777	27.27933398846548	22.511936462759557
66-67	22.552521396894562	27.744736813033484	27.164634688161787	22.53810710191017
68-69	22.45865356009791	27.660945758498627	27.30831106307909	22.57208961832437
70-71	22.511683272624865	27.45846183732995	27.355111370021167	22.674743520024013
72-73	22.60415552490104	27.521097189612227	27.256116478137425	22.61863080734931
74-75	22.34836576538203	27.685833682336376	27.317401155849787	22.64839939643181
76-77	22.543981557721178	27.611800537628305	27.25119953867832	22.593018365972192
78-79	22.461706571895775	27.64234124995007	27.239520286786757	22.656431891367404
80-81	22.505517081770588	27.696155090883906	27.229838930082646	22.568488897262856
82-83	22.50221431596315	27.63537175894389	27.363081352786704	22.49933257230625
84-85	22.579238795934266	27.676378167799754	27.23873982472515	22.505643211540832
86-87	22.40587864931359	27.718604991524764	27.34005412501961	22.535462234142035
88-89	22.441976343892073	27.676241543372015	27.324453952949934	22.557328159785982
90-91	22.45660796775144	27.775934459325896	27.318397548267537	22.449060024655125
92-93	22.500703956931016	27.713328935806654	27.355684302996643	22.430282804265687
94-95	22.34365735015139	27.825114102653416	27.35826520282349	22.472963344371706
96-97	22.399853490461176	27.954175019830046	27.387701219419462	22.258270270289312
98-99	23.479498138785626	29.893933385506127	27.54724166161486	19.07932681409339
100	22.45869254435463	30.844840239037698	21.287827956010965	25.40863926059671
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4615.0
1	3484.0
2	2562.5
3	2730.5
4	2671.0
5	2855.0
6	3347.5
7	3908.0
8	4362.0
9	4903.0
10	5617.5
11	6244.5
12	6870.0
13	7650.0
14	8631.5
15	10070.0
16	11908.5
17	14136.0
18	16971.5
19	20670.0
20	25598.0
21	32206.5
22	41126.5
23	52208.5
24	65990.5
25	84214.0
26	107636.0
27	132721.0
28	158225.0
29	186761.5
30	214050.5
31	240610.0
32	270255.5
33	298757.0
34	326535.5
35	352088.0
36	379719.5
37	407551.5
38	424962.0
39	436775.0
40	452112.5
41	466716.0
42	477084.5
43	488404.0
44	498055.5
45	508091.5
46	513887.5
47	515043.0
48	516666.0
49	514154.5
50	508419.5
51	498661.5
52	482836.0
53	460432.0
54	435809.0
55	410419.0
56	382529.0
57	350750.0
58	317328.5
59	282001.5
60	239533.5
61	196644.0
62	158656.5
63	125254.0
64	97061.0
65	75391.0
66	58438.5
67	45303.5
68	35140.0
69	26783.0
70	20454.5
71	15403.0
72	11455.0
73	8299.0
74	6038.0
75	4517.5
76	3449.5
77	2667.5
78	2076.5
79	1635.0
80	1266.0
81	961.0
82	746.5
83	515.0
84	354.5
85	270.5
86	217.0
87	169.5
88	105.0
89	61.5
90	45.0
91	40.5
92	32.5
93	22.5
94	14.0
95	10.0
96	10.0
97	7.5
98	2.5
99	6.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.8126998244070639E-4
6	0.0
7	0.0
8	8.239544656395745E-6
9	0.0
10-11	5.3557040266572346E-5
12-13	1.029943082049468E-4
14-15	4.9437267938374464E-5
16-17	9.887453587674893E-5
18-19	9.805058141110936E-4
20-21	3.7920314745207225E-4
22-23	0.0
24-25	9.080529520347444E-5
26-27	1.776437563507643E-4
28-29	0.0
30-31	0.0
32-33	5.4035818931124946E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	3.9897369779196434E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.911423046409955E-4
76-77	1.5271504441717066E-5
78-79	4.7935011258027054E-4
80-81	4.176540486939723E-5
82-83	3.701320758148934E-5
84-85	4.6043875423048186E-4
86-87	1.3014194202035496E-4
88-89	2.7479104339478174E-5
90-91	0.0
92-93	6.902322908883284E-4
94-95	3.9660731754295904E-4
96-97	3.699129048872893E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4029.0
20-21	7568.0
22-23	8796.0
24-25	10228.0
26-27	14211.0
28-29	20761.0
30-31	33123.0
32-33	38764.0
34-35	50898.0
36-37	74577.0
38-39	84275.0
40-41	83354.0
42-43	90946.0
44-45	102453.0
46-47	104876.0
48-49	103645.0
50-51	101222.0
52-53	101667.0
54-55	109388.0
56-57	111596.0
58-59	110177.0
60-61	109710.0
62-63	107470.0
64-65	113366.0
66-67	118790.0
68-69	119269.0
70-71	117966.0
72-73	114491.0
74-75	116765.0
76-77	120844.0
78-79	123141.0
80-81	122401.0
82-83	117880.0
84-85	117173.0
86-87	121703.0
88-89	126874.0
90-91	131188.0
92-93	135274.0
94-95	157954.0
96-97	396246.0
98-99	707399.0
100-101	7474135.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.28182734162169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.5656753309091	75.48758783975427
2	7.0646788683385555	11.90848089210627
3	1.8289814517028653	4.624496967703486
4	0.6988774923534742	2.356106885739242
5	0.3205780870465997	1.3509453490984444
6	0.17603676738201152	0.890202026056075
7	0.10083495756844667	0.59489881386485
8	0.0643949701097139	0.43418606019646383
9	0.044340440394934226	0.3363384007455584
>10	0.13180809160013013	1.7297863002085725
>50	0.0031855864542061642	0.1797568301063549
>100	5.744678847827592E-4	0.08390113254076922
>500	1.6746363886607402E-5	0.00811563136072333
>1k	1.674189125811363E-5	0.015196870518944562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.239544656395745E-6	0.0	0.0	0.0
3	0.0	8.239544656395745E-6	0.0	0.0	0.0
4	0.0	4.119772328197872E-5	0.0	0.0	0.0
5	0.0	6.591635725116596E-5	0.0	2.4718633969187236E-5	0.0
6	4.943726793837447E-5	6.591635725116596E-5	0.0	3.295817862558298E-5	0.0
7	6.591635725116596E-5	7.415590190756171E-5	0.0	4.119772328197872E-5	0.0
8	6.591635725116596E-5	8.239544656395744E-5	0.0	4.119772328197872E-5	0.0
9	8.239544656395744E-5	1.8950952709710214E-4	0.0	4.943726793837447E-5	8.239544656395745E-6
10-11	9.063499122035319E-5	1.9774907175349788E-4	0.0	9.475476354855107E-5	8.239544656395745E-6
12-13	9.063499122035319E-5	1.9774907175349788E-4	0.0	1.029943082049468E-4	8.239544656395745E-6
14-15	9.063499122035319E-5	3.666597372096106E-4	0.0	1.1535362518954041E-4	1.647908931279149E-5
16-17	1.3183271450233191E-4	4.119772328197872E-4	0.0	1.2771294217413405E-4	2.4718633969187236E-5
18-19	1.3183271450233191E-4	4.53174956101766E-4	0.0	1.5655134847151916E-4	2.4718633969187236E-5
20-21	1.3595248683052978E-4	4.8613313472734894E-4	0.0	1.8538975476890427E-4	2.4718633969187236E-5
22-23	1.4419203148692555E-4	5.602890366349107E-4	0.0	3.1310269694303827E-4	2.8838406297385107E-5
24-25	1.5655134847151914E-4	6.46804255527066E-4	0.0	6.385647108706702E-4	4.943726793837447E-5
26-27	1.8950952709710214E-4	6.838822064808468E-4	0.0	0.0014913575828076299	4.943726793837447E-5
28-29	1.8950952709710214E-4	6.838822064808468E-4	0.0	0.006583396180460201	4.943726793837447E-5
30-31	1.936292994253E-4	7.456787914038149E-4	0.0	0.02282353869821621	4.943726793837447E-5
32-33	2.101083887380915E-4	7.580381083884085E-4	0.0	0.061425805413430276	4.943726793837447E-5
34-35	2.224677057226851E-4	8.69271961249751E-4	0.0	0.10446094715378526	5.7676812594770214E-5
36-37	2.4306656736367447E-4	0.0010175837650648746	0.0	0.151842448700389	6.591635725116596E-5
38-39	2.4718633969187233E-4	0.0010752605776596447	0.0	0.19754308313708796	7.415590190756171E-5
40-41	2.554258843482681E-4	0.0012400514707875596	0.0	0.23715057430038233	8.239544656395744E-5
42-43	2.554258843482681E-4	0.0013059678280387256	0.0	0.27239110679578693	8.651521889215532E-5
44-45	2.554258843482681E-4	0.0014748784934948383	0.0	0.2992355432863243	9.063499122035319E-5
46-47	2.554258843482681E-4	0.0015902321186843787	0.0	0.3210414982194756	9.063499122035319E-5
48-49	2.5954565667646594E-4	0.0016025914356689723	0.0	0.33584795996701877	9.063499122035319E-5
50-51	2.6366542900466383E-4	0.001837418458376251	0.0	0.3466458832392254	9.475476354855107E-5
52-53	2.6366542900466383E-4	0.0018456580030326469	0.0	0.35480715222138537	1.1123385286134255E-4
54-55	2.6366542900466383E-4	0.0018538975476890425	0.0	0.3616459742861938	1.2359316984593616E-4
56-57	2.7190497366105955E-4	0.0018621370923454382	0.0	0.36821289137734126	1.2359316984593616E-4
58-59	2.7190497366105955E-4	0.0018868557263146254	0.0	0.374495544177843	1.2359316984593616E-4
60-61	2.8014451831745533E-4	0.0019156941326120107	0.0	0.3803332615668994	1.2359316984593616E-4
62-63	3.131026969430383E-4	0.0019568918558939895	0.0	0.3851492754185627	1.2359316984593616E-4
64-65	3.2134224159943405E-4	0.0021299222936783	0.0	0.38914133480458646	1.2359316984593616E-4
66-67	3.2958178625582977E-4	0.0021793595616166747	0.0	0.3927626146810723	1.2359316984593616E-4
68-69	3.2958178625582977E-4	0.0022452759188678405	0.0	0.39638801432988646	1.2359316984593616E-4
70-71	3.3370155858402766E-4	0.0022988329591344127	0.0	0.39948196334836306	1.2359316984593616E-4
72-73	3.6253996488141277E-4	0.002319431820775402	0.0	0.4023452051164606	1.2771294217413405E-4
74-75	3.707795095378085E-4	0.0023647493163855785	0.0	0.4047429126114718	1.3595248683052978E-4
76-77	3.748992818660064E-4	0.0024512645352777343	0.0	0.40729305168262625	1.4007225915872766E-4
78-79	3.7901905419420427E-4	0.0024965820308879105	0.0	0.4092829017171459	1.4007225915872766E-4
80-81	3.7901905419420427E-4	0.0024965820308879105	0.0	0.41008625732114445	1.4007225915872766E-4
82-83	3.913783711787979E-4	0.002508941347872504	0.0	0.4106053486344974	1.4007225915872766E-4
84-85	4.078574604915894E-4	0.002546019298826285	0.0	0.41083605588487637	1.4007225915872766E-4
86-87	4.119772328197872E-4	0.0025872170221082637	0.0	0.41088549315281475	1.4007225915872766E-4
88	4.119772328197872E-4	0.0027190497366105957	0.0	0.41090609201445577	1.4007225915872766E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10135	0.0	37.26763	1
GTATCAA	17010	0.0	32.05204	1
TCAACGC	19050	0.0	27.476778	4
ATCAACG	19520	0.0	26.858099	3
CAACGCA	19855	0.0	26.341671	5
AACGCAG	20275	0.0	25.837305	6
TATCAAC	21225	0.0	25.055714	2
ACGCAGA	24020	0.0	21.756666	7
CGCAGAG	24930	0.0	20.895313	8
GTACATG	40425	0.0	20.893194	1
TACATGG	40740	0.0	20.310316	2
ACATGGG	41835	0.0	19.288244	3
CATGGGG	30310	0.0	17.352196	4
GCAGAGT	31450	0.0	16.390348	9
AGAGTAC	25975	0.0	15.605245	10-11
TATACCG	965	0.0	15.187682	5
CAGAGTA	29795	0.0	14.658573	10-11
GTGGTAT	6515	0.0	13.754612	1
ATGGGGG	17340	0.0	13.740861	5
GTATAAG	5085	0.0	13.25818	1
>>END_MODULE