FastQCFastQC Report
Fri 27 May 2016
SRR2140233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9732443
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84800.043.12581
GTATCAA149900.033.81251
TCAACGC166900.028.9800324
ATCAACG173600.027.837293
CAACGCA175450.027.5197545
TATCAAC183550.026.9250742
AACGCAG181100.026.753976
ACGCAGA218150.022.1328957
GTACATG371050.021.4492841
TACATGG372300.020.8001162
CGCAGAG236450.020.4733778
ACATGGG379850.019.9208323
CATGGGG283150.017.7664664
GCAGAGT277700.017.0832849
AGAGTAC237100.015.64611910-11
CAGAGTA267350.015.56989210-11
ATGGGGA149650.014.0487315
TATACCG7800.014.0440395
GTATAAG40300.013.9046431
GTGGTAT50750.013.8641641
Produced by FastQC (version 0.11.5)