FastQCFastQC Report
Fri 27 May 2016
SRR2140232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7280222
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.049.974621
ATCTCGT72650.038.57470794
GTATCAA166250.036.4418751
TATCTCG87650.034.41452494
TCAACGC195450.030.319364
ATCAACG196100.030.1752553
CAACGCA200000.029.6295955
AACGCAG205500.028.9198116
TATCAAC207800.028.7437442
TATGCCG40900.026.7420886-87
ATGCCGT43300.026.66818288-89
CCGTCTT45450.025.16172290-91
GCCGTCT47250.025.07201490-91
TGCCGTC44850.025.0455288-89
ACGCAGA237850.024.9324977
CGCAGAG245350.024.20528
TCTCGTA46200.024.10032882-83
TCGTATG47900.023.8313784-85
CTCGTAT45600.023.64322982-83
CGCTATC133750.023.32689394
Produced by FastQC (version 0.11.5)