##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7280222
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.187911852138576	33.0	31.0	34.0	30.0	34.0
2	32.416116981048106	34.0	31.0	34.0	31.0	34.0
3	32.4622023888832	34.0	31.0	34.0	31.0	34.0
4	36.09124927783795	37.0	35.0	37.0	35.0	37.0
5	35.9484436051538	37.0	35.0	37.0	35.0	37.0
6	35.9699242687929	37.0	35.0	37.0	35.0	37.0
7	35.92670044951926	37.0	35.0	37.0	35.0	37.0
8	35.927136150518486	37.0	35.0	37.0	35.0	37.0
9	37.701532727985494	39.0	37.0	39.0	35.0	39.0
10-11	37.594534136459025	39.0	37.0	39.0	35.0	39.0
12-13	37.561757114000095	39.0	37.0	39.0	35.0	39.0
14-15	38.84389686193636	40.0	38.0	41.0	35.0	41.0
16-17	38.83106991792283	40.0	38.0	41.0	35.0	41.0
18-19	38.816983740331	40.0	38.0	41.0	35.0	41.0
20-21	38.8131759681127	40.0	38.0	41.0	34.5	41.0
22-23	38.74082512931865	40.0	38.0	41.0	34.0	41.0
24-25	38.68185667479541	40.0	38.0	41.0	34.0	41.0
26-27	38.51992257334929	40.0	38.0	41.0	34.0	41.0
28-29	38.395651953141396	40.0	38.0	41.0	34.0	41.0
30-31	38.276273329215485	40.0	38.0	41.0	34.0	41.0
32-33	38.187530230262595	40.0	38.0	41.0	34.0	41.0
34-35	38.23693222171181	40.0	38.0	41.0	34.0	41.0
36-37	38.197599222408805	40.0	38.0	41.0	33.5	41.0
38-39	38.13618936937158	40.0	38.0	41.0	33.0	41.0
40-41	38.06978405794169	40.0	37.5	41.0	33.0	41.0
42-43	38.044534994982946	40.0	37.0	41.0	33.0	41.0
44-45	38.082013909537366	40.0	37.0	41.0	33.0	41.0
46-47	38.07223628128148	40.0	37.0	41.0	33.0	41.0
48-49	37.99454628800042	40.0	37.0	41.0	33.0	41.0
50-51	37.911415073068156	40.0	37.0	41.0	33.0	41.0
52-53	37.689587822311296	40.0	37.0	41.0	32.5	41.0
54-55	37.57559486100228	40.0	36.0	41.0	32.0	41.0
56-57	37.51555977352831	39.0	36.0	41.0	32.0	41.0
58-59	37.36200140251724	39.0	36.0	41.0	32.0	41.0
60-61	37.170130650196086	39.0	35.0	41.0	31.5	41.0
62-63	36.92992578207359	39.0	35.0	40.0	31.0	41.0
64-65	36.64522757866624	38.0	35.0	40.0	31.0	41.0
66-67	36.36405580645624	37.5	35.0	40.0	31.0	41.0
68-69	36.08133671613188	37.0	35.0	40.0	30.5	41.0
70-71	35.78332284350358	36.5	34.0	39.0	30.0	41.0
72-73	35.469021474633706	36.0	34.0	39.0	30.0	40.5
74-75	35.09177831362657	36.0	34.0	38.5	30.0	40.0
76-77	33.7143464471183	34.5	32.0	36.5	28.0	39.0
78-79	34.39635595114579	35.0	33.5	37.0	29.0	39.0
80-81	34.31698107043073	35.0	34.0	37.0	29.5	39.0
82-83	34.029973491712965	35.0	34.0	36.0	29.0	38.0
84-85	33.86148311784055	35.0	33.5	36.0	29.0	37.0
86-87	33.63243699213497	35.0	33.0	35.5	29.0	37.0
88-89	33.527378760520975	35.0	33.0	35.0	29.0	36.5
90-91	33.36418722051717	35.0	33.0	35.0	29.0	36.0
92-93	33.26493387739914	35.0	33.0	35.0	29.0	36.0
94-95	33.24194945592629	35.0	33.0	35.0	29.0	36.0
96-97	33.26070519749018	35.0	33.0	35.0	29.0	35.0
98-99	33.34751692717174	35.0	33.0	35.0	29.5	35.0
100	32.10053040248606	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	37.0
21	197.0
22	928.0
23	3476.0
24	9110.0
25	20401.0
26	37371.0
27	60038.0
28	85440.0
29	115118.0
30	146455.0
31	182526.0
32	226628.0
33	286740.0
34	384976.0
35	536131.0
36	835687.0
37	1484628.0
38	1938125.0
39	891578.0
40	34625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.42567657964277	20.99043133574773	15.251526670477904	23.332365414131605
2	14.574844008877752	21.556183314190143	40.828040683374766	23.04093199355734
3	17.73079172585671	26.531553570756493	31.110218891676656	24.62743581171014
4	12.660465573714646	18.878215526944096	39.31623238961669	29.145086509724567
5	13.252123355578993	37.97848472203183	35.60694989795641	13.162442024432771
6	29.038839749666973	38.3315096709963	19.214208028271663	13.415442551065063
7	26.126552184809746	33.023498459250284	23.338148204821227	17.511801151118746
8	24.744108078022894	36.08639956309025	21.294721507118876	17.87477085176798
9	25.278583537699813	17.246589458398383	21.659394452531807	35.815432551369994
10-11	23.99494136305184	27.023104515219455	28.957626566882166	20.02432755484654
12-13	24.951237750716942	24.44485209379604	29.02204767931527	21.581862476171743
14-15	22.16874018402186	25.935362960085556	26.705071631057404	25.19082522483518
16-17	21.65491506761405	29.10438996811052	27.296211448564755	21.94448351571068
18-19	21.67508582847382	28.23936041475681	28.955465344581715	21.130088412187657
20-21	22.61317601072969	27.250450138104625	28.83524670645061	21.301127144715075
22-23	22.332391507867996	27.18228090468274	28.64896919534886	21.8363583921004
24-25	22.077421056099446	27.412017763919227	28.710693632305397	21.799867547675934
26-27	22.013593528823662	27.540822931343012	28.597870547876653	21.847712991956676
28-29	21.970025925375246	27.557027504224962	28.634218661951344	21.838727908448448
30-31	22.25714865557747	27.303724168544207	28.585129045067053	21.85399813081127
32-33	22.052374133281543	27.48500459340516	28.470114923098844	21.99250635021446
34-35	22.156336911150078	27.512650951149816	28.51023875085862	21.820773386841488
36-37	22.148023847084858	27.48898631262734	28.456778513992838	21.906211326294972
38-39	21.954008613216068	27.59896598950697	28.48088037953288	21.96614501774408
40-41	22.305728232053998	27.38178304561042	28.439071544730744	21.873417177604836
42-43	22.060222751100007	27.46306578983106	28.255477110380795	22.22123434868814
44-45	22.131232819665016	27.556597255770406	28.13738017026309	22.174789754301496
46-47	22.17660482392279	27.50380400492786	28.034912908298747	22.284678262850598
48-49	22.147424049496486	27.76054018749537	27.866491673510595	22.225544089497554
50-51	22.10221677632025	27.82420119877489	27.69905710996447	22.374524914940384
52-53	22.128100275743577	27.658570952924922	27.603164704821925	22.610164066509572
54-55	22.273249917199117	27.384785194796677	27.958596242258256	22.38336864574595
56-57	22.131150383233845	27.190933446222836	28.010889862867437	22.667026307675883
58-59	21.902254771425074	27.052632216647897	28.17271859116275	22.872394420764284
60-61	22.099281568916492	27.274482914851962	28.394396539695	22.23183897653655
62-63	21.633109073600128	27.509079608762843	28.386783259195557	22.471028058441473
64-65	21.463568563564493	27.830370750191424	28.043306137095815	22.66275454914827
66-67	21.569022808102822	28.352710676658898	27.550617747381445	22.527648767856835
68-69	21.61448622756865	28.379655239468406	27.41374462170798	22.59211391125496
70-71	21.61126403920192	27.853214502713524	27.385494890846505	23.15002656723805
72-73	21.821749655572916	27.351320692220305	27.411838258258868	23.41509139394791
74-75	21.918934202506865	27.39051445285437	27.56927126289943	23.121280081739336
76-77	22.25926013675044	27.291278247665325	26.977505369774352	23.471956245809885
78-79	22.428791146923018	27.316108439593823	26.562273305187666	23.692827108295493
80-81	22.277204734404307	27.712080104540902	26.716733108390827	23.29398205266396
82-83	22.265381529712077	27.495025376320875	26.81763355563626	23.42195953833079
84-85	22.38089280829833	27.874481831501676	26.757752648887966	22.986872711312028
86-87	22.80230217132709	27.744039112719694	26.829211925915526	22.624446790037695
88-89	22.53517065164872	27.843652537885365	27.177100755886517	22.444076054579394
90-91	22.500681432814265	27.4615388699897	27.08122856141568	22.956551135780355
92-93	22.492104424393702	26.99136961627147	27.336401342142093	23.18012461719274
94-95	21.671241535452726	27.491535452724808	27.998463093692923	22.838759918129547
96-97	21.74197535968288	27.37054585456956	28.25656962583337	22.630909159914193
98-99	22.953982153416618	29.270656648806387	28.611425245759133	19.163935952017866
100	22.068897788785154	30.50330819873755	22.058788972700434	25.36900503977686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	709.0
1	706.5
2	1171.0
3	1751.0
4	2220.5
5	3480.5
6	4757.0
7	5851.0
8	6893.5
9	7264.0
10	6830.5
11	6107.5
12	6472.5
13	8617.0
14	13787.0
15	21526.5
16	28419.0
17	32977.5
18	34881.0
19	33909.5
20	31108.5
21	28071.0
22	25919.5
23	25872.0
24	28969.0
25	35343.0
26	44923.5
27	56779.5
28	71298.0
29	89342.5
30	108701.5
31	128889.0
32	149888.0
33	170112.5
34	189800.5
35	207974.5
36	228205.0
37	247355.0
38	259874.5
39	269412.0
40	280087.0
41	289572.5
42	295205.0
43	300862.5
44	305732.0
45	311180.5
46	314623.0
47	315160.5
48	315538.5
49	311720.0
50	303778.0
51	294209.0
52	279512.5
53	260687.0
54	243135.5
55	223762.5
56	202672.5
57	180232.5
58	157059.5
59	135065.0
60	112878.5
61	91964.5
62	72495.0
63	55212.0
64	41691.5
65	31907.0
66	24885.5
67	19192.5
68	14441.0
69	10457.5
70	7337.5
71	5121.0
72	3498.5
73	2328.0
74	1529.5
75	1019.5
76	677.0
77	419.0
78	289.0
79	217.0
80	145.0
81	93.0
82	67.0
83	48.0
84	33.0
85	22.0
86	13.0
87	7.5
88	7.5
89	6.5
90	5.0
91	4.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0010713958997404201
18-19	5.49433794738677E-5
20-21	0.0
22-23	0.006841178537244757
24-25	0.008120444744804719
26-27	0.021339735391404765
28-29	0.016715489510463064
30-31	0.0021478136358629805
32-33	0.006471400995533284
34-35	0.033139721883758945
36-37	0.005580725965575052
38-39	0.0027442325031087336
40-41	0.007575208162016502
42-43	0.0074049665895970194
44-45	0.00812701779617111
46-47	0.005043172801218084
48-49	0.001112663668825913
50-51	0.0023471759256033253
52-53	9.197499751667507E-4
54-55	0.0052459298546973215
56-57	8.558123640245108E-4
58-59	0.0012707188116474087
60-61	7.665230366219404E-4
62-63	0.0012617971461303047
64-65	4.3216181594091783E-4
66-67	0.005331629254741645
68-69	0.004305623368693394
70-71	0.0016596908763113025
72-73	0.0015825472886889886
74-75	7.11759254549741E-4
76-77	8.086030839959903E-4
78-79	6.624453901692941E-4
80-81	9.270659810241182E-4
82-83	0.007323343198340266
84-85	7.802947088300609E-4
86-87	6.872969145007794E-5
88-89	4.530315214105311E-4
90-91	3.2744066974183607E-4
92-93	0.0023582432527915254
94-95	0.003266782729217114
96-97	6.59229428604417E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	450.0
22-23	947.0
24-25	2799.0
26-27	2870.0
28-29	6409.0
30-31	14731.0
32-33	20257.0
34-35	27993.0
36-37	40518.0
38-39	28757.0
40-41	50209.0
42-43	60296.0
44-45	66831.0
46-47	66335.0
48-49	60112.0
50-51	32869.0
52-53	9273.0
54-55	8857.0
56-57	9303.0
58-59	35698.0
60-61	62195.0
62-63	61513.0
64-65	66034.0
66-67	70675.0
68-69	70052.0
70-71	68684.0
72-73	66491.0
74-75	68707.0
76-77	68325.0
78-79	72751.0
80-81	74037.0
82-83	71699.0
84-85	74012.0
86-87	79320.0
88-89	86768.0
90-91	93476.0
92-93	103005.0
94-95	120989.0
96-97	295582.0
98-99	549341.0
100-101	4510914.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.89965085034021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.25829903152973	74.88740124240604
2	7.45224603397676	12.504816806029655
3	1.785782505512893	4.494795861215324
4	0.6664574642844522	2.2366219424027447
5	0.3057880956989758	1.2827757231667247
6	0.15847942187235087	0.7977820897232405
7	0.09842079662332032	0.5780229331176243
8	0.06873773787349112	0.46136577662624884
9	0.038522709468198635	0.2908837686771864
>10	0.16354076549569918	2.109565509544471
>50	0.0028710579016358044	0.1534092246487117
>100	7.43233586996386E-4	0.1328305137841439
>500	8.335965623633006E-5	0.04357165531805229
>1k	2.7786519254465415E-5	0.026156953339790047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.494337947386769E-5	0.0	0.0	0.0
3	0.0	5.494337947386769E-5	0.0	1.3735844868466923E-5	0.0
4	0.0	1.236226038162023E-4	0.0	1.3735844868466923E-5	1.3735844868466923E-5
5	0.0	1.236226038162023E-4	0.0	2.7471689736933846E-5	1.3735844868466923E-5
6	1.3735844868466923E-5	1.236226038162023E-4	0.0	2.7471689736933846E-5	1.3735844868466923E-5
7	1.3735844868466923E-5	1.236226038162023E-4	0.0	4.120753460540077E-5	1.3735844868466923E-5
8	1.3735844868466923E-5	1.236226038162023E-4	0.0	4.120753460540077E-5	1.3735844868466923E-5
9	1.3735844868466923E-5	1.3735844868466922E-4	0.0	9.615091407926846E-5	1.3735844868466923E-5
10-11	3.4339612171167305E-5	1.3735844868466922E-4	0.0	1.7169806085583653E-4	1.3735844868466923E-5
12-13	5.494337947386769E-5	1.4422637111890268E-4	0.0	2.1977351789547074E-4	2.0603767302700384E-5
14-15	6.181130190810115E-5	2.0603767302700384E-4	0.0	2.609810525008715E-4	2.7471689736933846E-5
16-17	6.867922434233461E-5	2.2664144032970423E-4	0.0	3.227923544089727E-4	2.7471689736933846E-5
18-19	7.554714677656807E-5	2.609810525008715E-4	0.0	4.8075457039634226E-4	2.7471689736933846E-5
20-21	8.241506921080154E-5	2.8845274223780537E-4	0.0	8.03546924805315E-4	2.7471689736933846E-5
22-23	8.241506921080154E-5	3.2966027684320614E-4	0.0	0.0017169806085583655	4.120753460540077E-5
24-25	1.3049052625043576E-4	3.2966027684320614E-4	0.0	0.0038048290285653376	4.120753460540077E-5
26-27	1.5109429355313615E-4	3.2966027684320614E-4	0.0	0.008241506921080154	5.494337947386769E-5
28-29	1.5109429355313615E-4	3.2966027684320614E-4	0.0	0.019566711015131133	6.867922434233461E-5
30-31	1.7169806085583653E-4	3.9833950118554076E-4	0.0	0.02176444619408584	6.867922434233461E-5
32-33	1.7856598329007E-4	3.9833950118554076E-4	0.0	0.026104973172521386	6.867922434233461E-5
34-35	1.7856598329007E-4	4.876224928305758E-4	0.0	0.03175040541346129	6.867922434233461E-5
36-37	1.7856598329007E-4	5.906413293440777E-4	0.0	0.04378987344067255	6.867922434233461E-5
38-39	1.7856598329007E-4	6.181130190810115E-4	0.0	0.19020024389366147	8.241506921080154E-5
40-41	1.7856598329007E-4	7.073960107260465E-4	0.0	0.343217555728383	8.241506921080154E-5
42-43	1.7856598329007E-4	7.486035453314473E-4	0.0	0.3713004905619636	8.241506921080154E-5
44-45	1.7856598329007E-4	8.172827696737819E-4	0.0	0.4085452339228117	8.241506921080154E-5
46-47	1.7856598329007E-4	8.653582267134162E-4	0.0	0.448592089636827	8.241506921080154E-5
48-49	1.7856598329007E-4	8.653582267134162E-4	0.0	0.4867708704487308	8.241506921080154E-5
50-51	1.9230182815853692E-4	9.271695286215173E-4	0.0	0.5220857276055593	8.241506921080154E-5
52-53	2.129055954612373E-4	9.340374510557508E-4	0.0	1.0164937278011577	8.241506921080154E-5
54-55	2.1977351789547077E-4	9.409053734899843E-4	0.0	1.688643560594718	8.241506921080154E-5
56-57	2.1977351789547077E-4	9.615091407926846E-4	0.0	2.42443843058632	8.241506921080154E-5
58-59	2.1977351789547077E-4	9.615091407926846E-4	0.0	3.1747383527590234	8.241506921080154E-5
60-61	2.2664144032970423E-4	9.615091407926846E-4	0.0	3.373393009169226	8.241506921080154E-5
62-63	2.335093627639377E-4	9.615091407926846E-4	0.0	3.381538365176227	9.615091407926846E-5
64-65	2.335093627639377E-4	0.0010988675894773538	0.0	3.3884818347572367	9.615091407926846E-5
66-67	2.609810525008715E-4	0.001146943046516988	0.0	3.3941890783000845	9.615091407926846E-5
68-69	2.609810525008715E-4	0.0011812826586881553	0.0	3.3999512652224064	9.615091407926846E-5
70-71	2.609810525008715E-4	0.0011950185035566223	0.0	3.404573377020646	9.615091407926846E-5
72-73	2.609810525008715E-4	0.0012018864259908558	0.0	3.4085293003427646	1.236226038162023E-4
74-75	2.609810525008715E-4	0.001236226038162023	0.0	3.4117572238868537	1.236226038162023E-4
76-77	2.609810525008715E-4	0.0012980373400701241	0.0	3.41506069457772	1.236226038162023E-4
78-79	2.609810525008715E-4	0.0013461127971097584	0.0	3.4172446939118064	1.236226038162023E-4
80-81	2.609810525008715E-4	0.0013461127971097584	0.0	3.4179177503103615	1.3049052625043576E-4
82-83	2.609810525008715E-4	0.0013529807195439918	0.0	3.4182680143545072	1.3735844868466922E-4
84-85	2.609810525008715E-4	0.0013941882541493927	0.0	3.418460316182666	1.3735844868466922E-4
86-87	2.609810525008715E-4	0.0014697354009259608	0.0	3.418556467096745	1.3735844868466922E-4
88	2.609810525008715E-4	0.0015521504701367624	0.0	3.418577070864048	1.3735844868466922E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9180	0.0	49.97462	1
ATCTCGT	7265	0.0	38.574707	94
GTATCAA	16625	0.0	36.441875	1
TATCTCG	8765	0.0	34.414524	94
TCAACGC	19545	0.0	30.31936	4
ATCAACG	19610	0.0	30.175255	3
CAACGCA	20000	0.0	29.629595	5
AACGCAG	20550	0.0	28.919811	6
TATCAAC	20780	0.0	28.743744	2
TATGCCG	4090	0.0	26.74208	86-87
ATGCCGT	4330	0.0	26.668182	88-89
CCGTCTT	4545	0.0	25.161722	90-91
GCCGTCT	4725	0.0	25.072014	90-91
TGCCGTC	4485	0.0	25.04552	88-89
ACGCAGA	23785	0.0	24.932497	7
CGCAGAG	24535	0.0	24.2052	8
TCTCGTA	4620	0.0	24.100328	82-83
TCGTATG	4790	0.0	23.83137	84-85
CTCGTAT	4560	0.0	23.643229	82-83
CGCTATC	13375	0.0	23.326893	94
>>END_MODULE