FastQCFastQC Report
Fri 27 May 2016
SRR2140231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6835828
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT90550.062.5866294
TATCTCG116250.050.09742494
GGTATCA62400.044.903941
CCGTCTT49650.041.048190-91
GCCGTCT51900.040.51235690-91
TGCCGTC50050.039.95013488-89
TATGCCG50850.038.68745886-87
ATGCCGT53350.038.3753588-89
TCGTATG58200.034.9479984-85
CGTATGC56600.034.5147384-85
CTCGTAT56750.034.0612482-83
TCTCGTA58950.033.69959382-83
GTATCAA108700.031.5057351
GTATGCC68350.030.3240186-87
CTATCTC139900.029.16711692-93
TCAACGC121700.027.117014
ATCAACG122600.026.8840873
CAACGCA125000.026.4343285
ACGCTAT242050.026.08230894
CGCTATC144850.025.866892-93
Produced by FastQC (version 0.11.5)